2003년 국내 중증급성호흡기증후군 진료 현황 및 문제점 분석

이진수,김은실,정문현,백제중,정선화,안주희,최영화,이선희,고철우,김성범,김민자,박승철,기현균,송재훈,최상호,김양수,이상오,조용균,박영훈,정숙인,김연숙,이흥범,손창희,장성희,정희진,김우주 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

목적 : 2002년 말 중국에서 SARS가 발생한 이후 국내에서도 2003년 10월까지 총 3명의 추정환자, 17명의 의심환자가 보고되었다. 향후 추가적인 SARS의 유행이 우려되는 상황에서, 그간의 SARS 환자 진료에 있어서의 실질적인 준비사항, 진료 현황 등에 대한 조사를 통해 문제점을 파악하여, 향후 더 나은 대비가 될 수 있도록 개선점을 제시하고자 하였다. 재료 및 방법 : SARS로 의심되는 환자를 진료 경험이 있는 병원의료진을 대상으로 2003년 10월에 설문조사를 실시하였다. 설문에는 SARS 환자 진료 시의 실질적인 조치, 진료 현황, 병실, 응급실 및 외래에서의 격리 시설과 준비사항, 보건당국의 관리와 지원에 관한 사항을 포함하였다. 결과 : 대상이 되는 22개 병원 중 17개(17/22, 77.2%) 병원이 설문에 응하였다. SARS 환자를 위한 격리실은 응급실, 외래, 일반병실 및 중환자실에서 각각 9개(9/17, 52.9%), 5개(5/17, 29.4%), 15개(15/16, 93.7%), 4개(4/16, 25%) 병원에서 음압처리가 되어있지 않은 일인실 혹은 다인실이 사용되었고, 1개(1/16, 6.3%) 병원에서만 일반병실에서 음압격리실이 운영되었다. 입원환자의 진찰 시 개인보호구의 착용은 거의 모든 의료기관에서 이루어졌다. 보건당국에서 SARS지정병원의 시설 등을 사전에 확인한 곳은 1곳(1/12, 8.3%)이였고, 14개 병원(14/15, 93.3%)에서는 보건당국에 의뢰한 검사결과를 통하 받지 못하였다. 결론 : 의료기관에서 SARS 환자용 격리실뿐만 아니라 기존의 격리실 설비 등이 미흡하였으며, 특히 중환자실 및 외래의 준비가 더욱 부족하였다. 보건당국의 의료기관에 대한 종합적인 지원이 부족하였고, 병원과의 원활한 연계가 잘 이루어지지 않았다. SARS 만이 아닌 격리를 필요로 하는 질환의 적절한 진료를 위해 향후 병원 시설의 정비와 정부차원에서의 보다 구체적이고 실질적인 대책마련이 필요하다. Background : There was an worldwide outbreak of the Severe Acute Respiratory Syndrome (SARS) originated from China in late 2002. During that period three cases of suspected SARS and 17 cases of probable SARS were reported in Korea. With the concerns about the reemergence of SARS-coV transmission, it is important to be prepared for any possibility. So, this study is aimed to analysis the past measures in managing SARS and propose the amendatory plans to improve the preparedness. Materials & Methods : Questionnaires were collected among clinicians with any experience in managing the probable or suspected SARS cases in Oct. 2003. 17 out of 22 hospitals responded to the questionnaire. The contents in the questionnaire were practical activities, personal equipments, response plans, isolation facilities in emergency centers, outpatient clinics, general wards and intensive care units, and relationship with the public health department. Results : The dedicated isolation rooms in emergency centers, outpatient clinics, general wards, and intensive care units were prepared in 9 (9/17, 52.9%), 5 (5/17, 29.4%), 15 (15/16, 93.7%), and 4 (4/16, 25.0%) hospitals, respectively. Except for one hospital that newly made negative pressure room for SARS, single or multi-bed rooms without airborne infection control were used in all the other hospitals. The personal precaution principles were kept quite well in general wards. Before the designation of SARS hospital by the public health department prior evalution to see if the hospital was suitable for managing SARS was conducted in only 1 (1/12, 8.3%) hospital. The results of laboratory diagnosis were reported back in 1 (1/15, 6.6%) hospital. Conclusions : The isolation facilities which can control airborne infection were almost deficient not only for SARS but also for other respiratory transmissible diseases. For the infection control of transmissible diseases including SARS, more investment is needed on medical facilities and comprehensive support from the public health department required.

한국 정상인에서 연령에 따른 뇌혈류분포와 혈류예비능의 변화 : Tc-99m HMPAO SPECT 에 의한 연구

문대혁,이희경,송호천,이재태,범희승,손혜경,정환정,민정준,김지열 대한핵의학회 1999 핵의학 분자영상 Vol.33 No.3

Purpose: The aim of this study was to evaluate the normal values of regional cerebral blood flow (rCBF) and cerebrovascular reserve (CVR) in normal children to aged volunteers using Tc-99m HMPAO. Materials and Methods: Thirty four right-handed normal volunteers (20 males, 14 females, mean age 40.3±24.9 years, range 4 to 82 years) were underwent rest/acetazolamide (ACZ) brain SPECT using Tc-99m HMPAO and the sequential injection and subtraction method. rCBF was estimated on the basis of a semiquantitative approach by means of right/left ratio, region/cerebellum and region to whole brain ratios in frontal, parietal, temporal, and occipital lobes, basal ganglia, thalami, and cerebellum. CVR was measured by means of % perfusion increase calculated as % mean count change compared to rest rCBF in each regions. Results: Mean values of right to left ratios range from 1.004 to 1.018. rCBF was highest in cerebellum and lowest in basal ganglia and thalami. Frontal and temporal rCBF decreased while occipital and thalami rCBF increased according to age. No sexual difference of rCBF was noted. Mean CVR was 29.9±12.9%. Mean CVR significantly increased to late teens, and declined thereafter. After 6th decade, CVR in both frontal lobes, left parietal lobe and right basal ganglia decreased significantly with advancing age. There was no sexual difference of CVR. Conclusion: Quantitative assessment of CVR was possible by ACZ Tc-99m HMPAO brain SPECT. It revealed that rCBF and CVR changed according to age in normal Korean volunteers. There was no sexual difference.

한우, 칡소 및 제주 흑우 Calpain-Calpastatin 유전자 다양성

이승환,김승창,조수현,최봉환,Aditi Sharma,임다정,당창권,장선식,김재환,고문석,양보석,강희설 충남대학교 농업과학연구소 2013 농업과학연구 Vol.40 No.2

The aim of study was to investigate genetic diversity for the calpain/calpastatin gene in three Hanwoo breeds [(Brown (n=62), Brindle (n=81) and Jeju Black (n=30)]. Random samples from three breeds of Hanwoo were selected and genotyped for the 7 SNPs of calpain/calpastatin using TaqMan method. Allele frequencies were investigated for CAPN1/CAST gene. Allele frequency of CAST2 SNP was 0.75, 0.59 and 0.22 for Brown, Brindle and Jeju black, respectively. The CAST3 revealed allele frequency of 0.59 and 0.57 in Brown and Jeju Black, while it showed very low allele frequency (0.07) in Brindle. In particular, favorable allele (G allele) for the CAPN1-2 SNP which was shown a strong association with tenderness in Taurine and Indicine cattle revealed 16% and 17% higher allele frequency in Brown Hanwoo (0.82) comparing Brindle (0.66) and Jeju Black Hanwoo (0.65). AMOVA demonstrated that among population variance occupied only 10% of total variance and among individual variance was 0%, while within individual variance was 90% of total variance. This result showed that population effect contributed very small portion of genetic to these three Hanwoo breeds, while within individual variance contributed large portion of genetic diversity within these Hanwoo breeds. In conclusion, three Hanwoo breeds (Brown, Brindle and Jeju black) showed a genetically homogeneous based on the 7 SNPs of CAPN1/CAST gene and it came from same ancestor to form modern Hanwoo breed.

소아 요로감염 환자에서 분리된 Extended-spectrum β-lactamase 생성 Escherichia coli의 위험인자 분석

문희원,이미애,이승주 대한임상미샐물학회 2003 Annals of clinical microbiology Vol.6 No.2

젊은 성인에서 신체 만족도에 따른 문제해결양식, 대인관계문제와 우울증상과 자살사고와의 관련성

차승민,김태성,강문희,권명진,김정란,왕성근,지익성 대한생물치료정신의학회 2011 생물치료정신의학 Vol.17 No.2

Objectives:The aim of this study was to evaluate that problem solving styles, interpersonal problems, depression and suicidal ideation according to body image satisfaction. Methods:One hundred and nineteen young healthy normal subjects filled out administered the Body Dysmorphic Disorder Examination-Self Report (Korean version of BDDE-SR K-BDDE-SR), Problem Solving Style Scale(Korean version PSS), Korean Inventory of Interpersonal Problem(KIIP), Korean version of the Depression Anxiety Stress Scale(KDASS-21), Korean version of Beck Scale for Suicidal Ideation(Korean version SSI) and Korean version of Reynolds Suicidal Ideation Questionnaire(Korean version SIQ) for a month. Results:Out of the 119 subjects, 39%(N=46) was male and 61%(N=73) was female. In high K-BDDE-SR group (higher than 67), helplessness was significantly higher than low K-BDDE-SR group(lower than 67), and problem-solving confidence, creative style, approach style were significantly lower than low K-BDDE-SR group. Also, Higher level of all KIIP factors in High K-BDDE-SR group was significantly differ than low K-BDDE-SR group. In High K-BDDE-SR group, K-DASS-21, SSI, and SIQ were significantly higher than low K-BDDE-SR group. Using multiple regression analysis contributing variance toward the total factor score of K-BDDE-SR of participants were K-DASS-21 and SIQ. Conclusion:Our results indicates that high tendency of dissatisfied body images have differences in helplessness, problem-solving confidence, approach style, interpersonal problems, depression and suicidal ideation compared to low tendency of dissatisfied body images. It is necessary to study body dysmorphic disorder patients group in the future.

Increased expression of P53 and Bax in the spinal cords of rats with experimental autoimmune encephalomylitis

문창종,김승준,위명복,김희석,정종태,박전홍,지영흔,Tanumab, Naoyuki,Matsumoto, Yoh,신태균 濟州大學校 農科大學 動物科學硏究所 2000 動物科學論叢 Vol.15 No.1

The expression of pro-apoptotic molecules, p53 and Bax, in the spinal cord of rats with experimental autoimmune encephalomyelitis (EAE) was examined. Apoptosis was confirmed by the terminal deoxynucleotidyl transferasemediated dUTP nick end-labeling (TUNEL) method. TUSEL (+) apoptotic cells were mainly either ED1 (+) macrophages or T-cells in the parenchyma of E N . Western blot analysis showed that both p53 and Bax expression significantly ( p < 0.01) increased in the spinal cords of EAE rats at the peak stage, and thereafter declined. An immunohistochemical study showed that inflammatory cells (notably T cells) in the parenchyma express p53 and Bax, while brain cells, includng neurons and glia, were devoid of these nuclear staining of these molecules. The nuclear expression of p53 largely matches apoptotic cells in the parenchyma of EAE. These finchngs suggest that pro-apoptotic molecules, p53 and Bax, may play an important role in eliminating T cells in the parenchyma in EAE.

간염 B형 형질전환동물 제조를 위한 간염 B형 바이러스 유전자의 조작

문애리,김승희 德成女子大學校 1996 德成女大論文集 Vol.27 No.-

Hepatitis B virus (HBV) infection is one of the serious problems in Southeast Asia including Korea because it causes acute and chronic hepatitis, which can easily be transformed to fatal conditions such as cirrhosis and hepatoma. Even though lots of informations on structural characteristics and hepatoma. Even though lots of informations on structural characteristics and gene expression mechanisms have been accumulated, the mechanism for HBV-induced hepatocellular injury which is believed to be the consequences of the ummunological response is not well understood. In order to perform immunopathological sutdies for prevention and treatment of HBV infection, we designed transgenic mice as a disease model which and mimic HBV infection. In this study, a promoter-HBV DNA fragment for the prepatation of HBV transgenic mice has been constructed. To add a proper enzyme site on 5' end of HBV gene, total HBV (subtype adr) gene was inferted into BamHI site of pBluescript SK vector and reeztracted by BamHI-PstI treatments. Promoter-HBV DNA was constructed by ligation of two fragments using identical PstI sites. For large scale production of promoter -HBV DNA, it was inserted to BamHI-SacI site of pBluescript SK vector, resulting in the construction of pBSKPH. The pBSKPH will be introduced to mouse embryo in the future to make HBV-transgenic mice which would make a useful animal model for studying HBV infection.

5-〔3-(N-Substituted Piperazinyl)Propoxy〕Indan類의 合成과 中樞神經抑制效果

黃熙喆,閔庚鎭,文勝煜,李萬佶,徐丙天 嶺南大學校附設 基礎科學硏究所 1985 基礎科學硏究 Vol.5 No.-

Five new indans, 5-[3-(N-substitued piperaziny) propxy]indans, were synthesized by condensation of 5-(3-bromopropoxy)indan, the intermediate, and N-substituted piperazines. The structure of the compunds were determined by means of IR, NMR spectoscopy along with elemental analysis. Each compound was tested for antimorphine and antiamphetamine effects as part of CNS depressant effects and for muscle relaxant action in mice. Thes compound exhibited less potent activity compared to chlordiazepoxide on CNS depressant effect. Muscle reaxant action of the compounds, the side effect of tranquilizer, however, was much weaker than that of chlordiazepoxide.

측정방법에 따른 콩 성분의 항산화효과 비교

문갑순,권태완,류승희 한국콩연구회 2003 韓國콩硏究會誌 Vol.20 No.1

콩 성분의 항산화효과를 측정을 위해 DPPH법, FRAP법, TEAC법을 이용하여 이들 방법간의차이를 비교하고, 콩의 항산화효과를 측정하는데 가장 적합한 실험방법을 확립하고자 하였다. 콩의 유효 성분 중 항산화효과와 관련있는 isoflavone 및 phenolic acid의 표준품을 이용하여 항산화효과를 측정하였고, 항산화효과의 비교를 위해 Trolox, ascorbic acid, glutathione의 활성을 측정하였다. DPPH법, FRAP법, TEAC법의 3가지 항산화효과 측정방법에 따른 genistein의 효과가 가장 높았고 DPPH나 FRAP의 경우는 gentisic acid, syringic acid, ferulic acid 등의 phenolic acids의 활성이 높게 나타났고 isoflavones의 효과는 매우 낮게 나타났다. 항산화효과를 측정하기 위해 다양하게 이용되고 있는 방법들간에는 차이가 있음을 알 수 있었으며 TEAC법의 경우 glutathione 및 콩의 주요 성분인 isoflavones과 대부분의 phenolic acids의 활성을 평가할 수 있어 유용한 방법으로 여겨진다. To establish the proper assay for measurement of antioxidative activities in soybean, the main components relating to their antioxidative effects were measured by DPPH (α, α-diphenyl-β-pycryl-hydrazyl radical), FRAP (ferric reducing/antioxidant power), and TEAC (Trolox equivalent antioxidant capacity) assay. The antioxidative activities of commercial isoflavones (genistein, daidzein, and glycitein) and phenolic acids, which were well known as main antioxidative ingredients in soybean, were measured by three methods and compared the activities with Trolox, ascorbic acid, and glutathione as reference antioxidnats. By the FRAP and DPPH assay, gentisic acid, syringic acid and ferulic acid showed the strong antioxidative activities, while isoflavones and glutathione showed very low or undetectable activities . In contrast genistein, p-coumaric acid, and ferulic acid showed remarkable antioxidative activities by the TEAC assay. Therefore. it is proposed that TEAC assay is suitable for detecting antioxidative activity of soybean.

김치식이가 흰쥐의 항산화시스템에 미치는 영향

류승희,권명자,송영옥,송영선,문갑순 인제대학교 2003 仁濟論叢 Vol.18 No.1

김치는 배추를 주재료로 하여 마늘, 고추, 생강 등의 양념류를 포함하는 여러 재료들로 만들어지며 이들 재료 중에는 항산화효과를 가진 것으로 알려진 성분들이 많이 함유되어 있어 김치도 항산화효과를 나타낼 것으로 여겨진다. In vitro 실험계 및 세포 모델계에서 김치 재료 및 김치의 항산화효과는 확인된바 있으나 In vivo 시스템에서의 김치의 항산화효과에 관한 자료는 부족하다. 따라서 본 실험에서는 김치 섭취의 생체 내 항산화효과를 규명하기 위하여 흰쥐에게 동결건조한 김치 3, 5, 10% 첨가 식이를 6주간 섭취시키면서 간조직의 산화와 항산화효소계의 활성을 측정하였다. 간의 MDA 함량은 대조군에 비해 김치 투여군에서 낮았고 특히 5% 김치섭취군에서 현저히 낮은 값을 나타내었다. 단백질 산화를 나타내는 카르보닐 함량도 대조군에 비해 김치섭취군에서 낮아 생체 내에서 김치식이가 과산화물 생성 억제효과를 나타내었다. 항산화 효소계의 활성을 측정한 결과 SOD 활성은 대조군에 비해 김치섭취군에서 현저하게 높았고 김치첨가 함량이 증가할수록 SOD 활성이 유의적으로 높아져 10% 김치섭취군에서는 대조군에 비해 70% 이상 높은 활성을 나타내었다. Catalase 활성은 각 군에서 유의적인 차이가 나타나지 않았으나 GSH-px의 활성은 김치 섭취가 높을수록 상대적으로 높은 증가율을 나타내어 SOD 활성과 동일한 경향을 나타내었다. 따라서 김치식이는 생체 내에서 치질 및 단백질 과산화를 현저하게 억제하였고 항산화효소계인 SOD와 GSH-px의 활성을 농도의존적으로 증진시켜 김치식이가 체내의 산화적 스트레스를 효과적으로 억제하는데 기여함을 알 수 있었다. Kimchi is a Kruean traditional fermented food containing various ingredients such as Chinese cabbage, garlic, ginger, red pepper and fish sauce. Some of their components were known as its antioxidative activities and antioxidative effects of kimchi have been studied using in vitro and cell model system. To investigate the antioxidative effect of dietary kimchi, 4 groups of male S.D. rats (n=36) were fed 0, 3%, 5%, and 10% freeze-dried kimchi for 6 weeks and hepatic lipid peroxidation, protein oxidation and the activities of antioxidative enzymes were measured. Hepatic MDA content fed kimchi diet was lower than control diet. Protein carbonyl values were decreased in kimchi diet group, but not significant. SOD activities were significantly increased in kimchi added groups at dose-dependent manner. At 10% kimchi added group, SOD activity increased by 70% compared with control diet group. Catalase activities were not different between experimental groups and control group, whereas GSH-px activities were significantly increased in kimchi added group. As a result kimchi diet inhibited lipid and protein oxidation and induced antioxidative enzymes, SOD and GSH-px in rat hepatic tissue. It suggests that dietary kimchi is expected to prevent the oxidative stress in animal and human.