Ribosomal DNA의 ITS영역 염기서열 분석에 의한 기주특이적 독소 생성 Alternaria spp.의 계통분류학적 유연관계

高昇柱,洪承範,姜熙玩,劉勝憲,柳震彰 충남대학교 생물공학연구소 1998 생물공학연구지 Vol.6 No.-

기주특이적 독소 생성 Alternaria spp.와 비병원성 A. alternata의 계통분류학적 유연관계를 규명하기 위하여 기주특이적 독소 생성 Alternaria 6균, 비병원성인 A. alternata 그리고 비교균으로서 소형포자를 형성하는 A. brassicicola, 대형포자를 형성하는 A. solani의 ribosmal DNA의 5.8S를 포함한 ITS영역의 염기 서열을 분석하였던 바 다음과 같은 결과를 얻었다. 1. Alternaria spp.의 rDNA ITS영역의 염기서열 크기는 548∼562bp였으며 5.8S rDNA에서는 비교종간에 서로 일치하였고 ITS I에서는 종간 뚜렷한 차이를 보였다. 2. 기주특이적 독소 생성 Alternaria spp.의 rDNA의 ITS영역의 염기서열은 비병원성인 A. alternata의 염기서열과는 서로 일치하였으나 A. brassicicola와는 3%, A. solani와는 7%의 염기서열 차를 보였다. 3. 기주특이적 독소 생성 Alternaria spp.는 비병원성 A. alternata와 진화적으로 매우 가까운 유연관계를 나타내었다. The regions of internal transcribed spacer(ITS) including 5.8S ribosomal DNA(rDXA) from six host-specific toxin(HST) producing Alternaria spp. nonpathogenic A. alternata. A. solani and A. brassicicola were sequenced to assess their relationship in taxonomy. There was no difference in the sequences of ITS including 5.8S rDNA among the HST-producing Alternaria spp. and nonpathogenic A. alternata. However, the sequences of HST-producing Alternaria spp. were different from those of A. solani and A. brassicicola by about 7% and 3%, respectively. These results suggest that HST-producing Alternaria spp. are evolutionarily very close to non pathogenic A. alternata although they have host-specific pathogenecity.

여말선초 시학사의 구도를 위하여 -방법으로서의 전고(典故)-

김승룡 ( Kim Seung-ryong ),류재민 ( Ryu Jae-min ) 한국한문학회 2017 韓國漢文學硏究 Vol.0 No.65

이 글은 여말선초 시학사의 구도를 준비하기 위한 방법을 모색하고자 하였다. 기존 시학사의 구도는 하나의 線을 이루지만, 그것이 개별 작품을 통해 구체적으로 검증해 낼 수 있는지는 확실하지 않다. 더구나 여말선초는 일찍이 하나의 흐름으로 파악되어 본 적이 없는 바, 전철을 밟지 않고 새롭게 준비될 방법이 필요하다. 이에 여말선초 시인으로 꼽히는 유방선을 중심에 놓고, 시학전통의 계승과 단절을 읽는 방법으로 `典故`를 주목하였다. 유방선은 고려말의 이색ㆍ이숭인ㆍ권근ㆍ변계량(`선배`)의 계보를 이어 받아, 서거정ㆍ 이승소ㆍ성간(`후배`) 등 조선초를 대표하는 문인들을 길러내었다. 유방선은 창작에 있어 표절 혐의를 받을 정도로 앞선 이들의 시문을 적극 차용했는데, 이는 그가 선배들의 시적 성취를 계승해 자신의 창작의 근거로 삼았음을 보여준다. 실제 작품을 통해 검증한 결과, 유방선이 선배의 시적 성취를 차용하여 후배에까지 전수된 시적 표현이 있는 반면, 후배에게 선택받지 못한 표현도 있음을 확인할 수 있었다. 특정한 시적 표현이 계승되고 단절되는 사이에서 유방선이 연결고리 역할을 했던 것이다. 한시에 있어 전고를 관류하는 핵심원리는 `번역`이라고 할 수 있다. 고려들은 전고 를 번역함으로써 전고 출발지의 서사를 전유하였고, 이는 `또 하나의 전통`(`한국적 전고`)이 되어 그들의 창작을 풍요롭게 했다. 그 창작은 다시 `또 하나의 전고`가 되었고, 조선인들은 이 전고를 번역하여 그들의 시공간에서 창작하였다. 이처럼 전고의 계승과 단절은 새로운 전고의 창신과 파괴로 이어지면서 한반도의 시학사를 구성하고 있었던 것이다. 차후 `전고와 번역`을 화두로 하여 한국한시사의 맥락을 찾고, 그 시적 전통의 계승과 단절을 추적할 수 있기를 기대한다. The purpose of this study is to find a way to newly construct history of poetics in late Goryeo and early Joseon. In the event of understanding composition of the poetry history through the existing way, the big stream can be comprehended but it cannot be confirmed that it applies to individual works. Particularly, a new way is required for this since it has never been figured out as a single stream in late Goryeo and early Joseon. This study will try to grasp the meaning of the succession and discontinuity of the poetic tradition through the `authentic precedent` based on a model of the poet, Yu Bang-Sun in late Goryeo and early Joseon. Yu Bang-Sun inherited the genealogy of Lee Saek, Lee Sung-In, Kwon Geun, and Byeon Gye-Ryang in late Goryeo, and brought up the literary persons who are representing Seo Geo-Jeong, Lee Seung-So, Seong Gan in early Joseon. Yu Bang-Sun has actively borrowed the works of his predecessors, enough to be accused of plagiarism when creating poetry. This shows the basis of his own creation by succeeding in the poetic achievements of his predecessors. As a result of verifying through actual works, the poetic tradition borrowed by Yu Bang-Sun continued to the next generations and stopped at him. Yu Bang-Sun played the role of connecting link while the tradition of the poetry history was succeeded and discontinued. The core principle of the authentic precedent in terms of Chinese poem is "translation". Authentic precedent translated by Goryeo`s authors has become a `new tradition` (`Korean authentic precedent`) and enriched their creative work. The creations that were born in such way became `authentic precedent` again. The authors of Joseon made their own creations through translating this new authentic precedent. Thus, the history of Korean poetics was made up through succession and discontinuity of authentic precedent. It is desired that we will find out the context of history of Korean poetics and keep track of the succession and disconnection of the poetic tradition based on the keyword of `authentic precedent and translation` in the future.

金井山 林道開設에 따르는 問題点에 관한 硏究

이경재,류창희,김승환,박승범 東亞大學校生命資源科學大學附設 農業資源硏究所 1993 農業生命資援硏究 Vol.2 No.1

Mt. Gum-Jung is a very important mountain of Pusan which is connected to the large branch of Mt. Baek-Du, range including Mt. Baek-Du, Mt. Un-Ju, Mt. Ka-Zi, Chui-Seo and Mt. Gum-Jung. It is also attached to Nak-Dong river linked to Mt. Baek-Yang and Mt. Seung-Hak. Regarding the Korean peninsula as one natural ecosystem, the large branch of Mt. Gum-Jung. Therefore, it is very important to primarily preserve it from ecological point of view. In case of constructing the forest road, the width of the road is 4 meters approximately, but the actual damaged width of the forest which includes the shoulder of both sides of the road, drainage ditches and the areas cutting the lower plants of trees, will be around 20∼28 meters. Since the forest road paved with concrete pavements and the gravel divides the ecosystem of the forests and thus destroys the ecological system, it should be paved with the natural materials, such as soils, herbals and so forth. The forests of Mt. Gum-Jung which have almost been in the 2nd transitional stage, have some areas showing active ecosystem restoration, such as Carpinus spp., so they should be preserved very thoroughly. The marshy ecosystem of about 27,720㎡ which is at the height of 600meters above sea level, west to North Gate of Mt. Gum-Jung Castle, has the high watertable formed naturally. The existence of the marshy at the near of the mountain top is a remarkably rare phenomenon, the preservation of as the marshy ecosystem is absolutely necessary. At present, this area is completely destroyed by the thoughtless use. Furthermore, the construction of the drainage due to the forest roads may cause the change of the waterable height of the marshy ecosystem. The construction of the forest road near to the to of the mountain, having th egood landscape and the historic relics, results in many problems with the landscape. Putting together, the construction of the forest roads should consider the totality of Mt. Gum-Jung instead of only considering forest preservation. Also the scientific method of a sustainable use should be sought, which fully incorporates the problems such as the width and size of the forest roads, the pavement methods and materials, and the choice of sites without ecological and landscape problems.

간경변증 환자의 정신과적 특성 및 아임상적 간성뇌병증 진단을 위한 연구 : 인지기능 및 유발전위를 중심으로 Cognitive Functions and Evoked Potentials

유승호,이소영,김진세,변관수,정인과 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.5

연구목적 : 본 연구는 명백한 간성뇌병증이 없는 간경변증 환자에서 불안, 우울기분 및 삶의 질 등 정신과적 특징과 신경심리학적인 결손의 양상을 밝히기 위해서 고안되었다. 그리고 아임상적 간성뇌병증의 평가에서 체성감각 유발전위 검사의 역할을 연구하고자 하였다. 방 법 : 간경변증으로 진단된 41명의 환자군과 정상 대조군 31명을 대상으로 하였다. 이들에서 우울기분 및 불안을 평가하기 위해 Beck Depression Inventory와 State & Trait Anxiety Inventory를 시행하였고 삶의 질의 평가를 위해 Health-related Quality of Life Questionnaire를 시행하였다. 또한 어휘검사, 숫자외우기 검사, 토막짜기 검사, 숫자기호 바꿔쓰기 검사 및 선로잇기 검사 A와 B 등 간단하지만 포괄적인 인지기능검사를 시행하였다. 또한 41명의 환자군 중에서 인지기능검사 결과에 의거하여 분류된 13명의 아임상적 간성뇌병증군(SHE군)과 11명의 아임상적 간성뇌병증이 아닌 간경변증 환자군(non-SHE군) 그리고 8명의 정상 대조군에서 체성감각 유발전위를 측정하였다. 결 과 : 간경변증 환자는 정상인에 비해서 우울기분과 특성불안이 유의하게 증가하였다. 또한 건강과 관련된 일상생활의 삶의 질의 수준이 정상인보다 저하되었고, 특히 주의력과 각성이 요구되는 행동에서 수행이 저하되었다. 또한, 간경변증 환자는 숫자기호 바꿔쓰기 검사와 선로잇기 검사에서 정상 대조군보다 저하된 수행을 나타내었다. 또한 이러한 인지기능검사를 통해서 진단한 아임상적 간성뇌병증군에서 6개월 추적조사를 하였을 때, 명백한 간성뇌병증의 빈도가 아임상적 간성뇌병증이 아닌 군보다 유의하게 높았다. 체성감각 유발전위 소견은 N65 잠복기와 N20-N65 IPL의 경우 SHE군, non-SHE군 및 정상 대조군 사이의 유의한 차이가 있었다. 특히 N65는 SHE군만이 정상 대조군과 유의한 차이가 있었고 N95는 숫자기호 바꿔쓰기 검사 및 선로잇기 검사 A와 유의한 상관관계가 있었다. 결 론 : 간경변증 환자에게 우울 및 불안 등 정서적인 어려움에 대한 실제적인 관리 및 삶의 질을 높일 수 있는 치료적 접근이 고려되어야 하겠다. 또한 숫자기호 바꿔쓰기 검사와 선로잇기 검사가 아임상적 간성뇌병증의 조기 진단에 민감하고 유용한 도구로 생각되어 간경변증 환자에서 정례적으로 적용시키는 것이 간경변증 환자의 관리에 도움을 줄 수 있을 것으로 생각된다. 마지막으로, 본 연구에서의 체성감각 유발전위 소견은 간경변증 환자에서 아임상적 간성뇌병증 환자의 진단 및 연구에 중요한 의미를 지닌다고 생각된다. Objectives : This study was designed to elucidate the psychiatric characteristics and nature of the neuropsychological deficits associated with subclinical hepatic encephalopathy(SHE) in patients with non-alcoholic liver cirrhosis. And also the authors prospectively studied the role of somatosensory evoked potentials(SEP) in the assessment of SHE. Methods : Beck Depression Inventory(BDI) for measuring depression, State Trait Anxiety Inventory(STAI) for measuring anxiety, and Health-related Quality of Questionnaire(HQLQ) for measuring quality of life were administered the 41 non-encephalopathic liver cirrhosis patients(NELC) group and 31 carefully matched normal controls. And a prospective study to compare the performance of NELC group and normal controls on a short but comprehensive cognitive tests was conducted. After several cognitive, NELC group was divided into two groups, SHE group and non-SHE group by results of digit symbol substitution test(DSST) and/or trail making test(TMT) A and B. And then median nerve evoked cortical responses in 13 SHE patients group, 11 non-SHE patients group, and 8 normal controls were recorded for latencies of N13, P16, N20, P25, N30, P45, N65, and P95. And also responses for N13-N20 interpeak latency(IPL) and N20-N65 IPL were recorded. Results : There were significant differences between the NELC group and normal controls on BDI, trait anxiety scales in STAI, and almost all HQLQ except for the cognitive function, social interaction and life satisfaction(p<0.05). The NELC group exhibited poor performance in DSST, TMT A and TMT B compared with normal controls(p<0.05). As 17 NELC patients had abnormal cognitive test results, 41.5% of cirrhotic patients had SHE. In SEP assessment, NELC group with SHE and without SHE had higher N20-N65 IPL and only NELC group with SHE had higher N65 latency compared with normal controls(p<0.05). Conclusion : This study suggests that the patients with liver cirrhosis exhibit relatively selective deficits in complex attentional and fine motor skills, with preservation of general intellectual ability, memory, language and visuospatial perception. DSST, TMT A and TMT B seem to be useful screening tests for the detection of SHE. And also it is expected that late components and N20-N65 IPLs of SEP are helpful in the assessment of SHE.

흑연-메소페이스핏치 복합재료의 열이방성

유승곤,김영일,이보성,주혁종 忠南大學校 産業技術硏究所 1986 산업기술연구논문집 Vol.1 No.1

In the development of thermal anisotropic materials with natural graphite powder and pitches as raw materials, extruding and hot-press method were investigated. Pitches should be reformed to mesophase pitch precursor by thermal pretreatment for using as a binder, and the heating rate was recommended within 0.2℃/min in the carbonization process. The tensile strength of axial direction of all specimens was about three times larger than that of radial direction and it could be increased about 25-60% by adding 1% chopped carbon fiber. The thermal expansion coefficient of axial direction was about 1/3-1/5 than that of radial direction and the thermal expansion was repressed by adding carbon fiber. The thermal conductivity of carbon fiber added specimens was increased with increasing the temperature, while that of without carbon fiber was decreased. The thermal conductivity of axial direction was about 8.0-4.58 times larger than that of radial direction by adding carbon fiber in the range of 0-400℃. As a general, properties of hot-pressed specimens were better than those of extruded specimens.

Attrition Reactor에서의 힘의 소모량 측정에 관한 연구

劉承坤 충남대학교 공업교육연구소 1982 論文集 Vol.5 No.1

The milling effect of Attrition reactor was very successful using stainless steel balls as a milling media. But Attrition reactor need large amount of power consumption to agitat the heavy milling media. Power measurements were studied with a dynamometer which was attached to the torque table. Power consumptions were measured by changing agitation speeds, kinds of milling media, heights of milling media, positions of stirrer, stirrer types, and so on.

植物體의 水溶性 成分의 抽出 메카니즘

유승곤,송봉준,이보성 충남대학교 공업교육연구소 1985 論文集 Vol.8 No.1

Water soluble components of Persicaria hydropiper have an insecticidal effect. The extraction mechanism of water solubles from Persicaria hydropiper was studied and it turned out to be a diffusion model. The diffusivity was logarithmically increased with respect to extraction temperature (20…70℃) showing the values in 0.7…2.65×10^-7 cm²/min. Some variables which have an effect on extracted quantity of water solubles were also observed. As a result, optimum operatin conditions and empirical equation were presented for the industrial scale extraction.

Lady Macbeth의 양성적 특성

류승희 대불대학교 1999 論文集 Vol.5 No.1

This paper aims mainly to examine the masculinity of Lady Macbeth and its destructive influence upon Macbeth's tragic end, although she has both masculinity and feminity. Masculinity means such qualitied found in Elizabethan literature as ambition for power, aggression, boldness, and prowess. But Lady Macbeth demands brutal masculinity of Macbeth. She scolds him for his mildness and urges him to murder Duncan. She also exercises this kind of masculinity herself to accomplish her strong ambition for power, thus leading and mastering him. From an Elizabethan patriarchal point of view such strong, aggressive and domineering women as Lady Macbeth were the opposite of ideal women, because the most important feminine quality in those days was submissiveness. She is such an unusual woman as Elizabeth 1 and the female ones who are shrewd, astute and intelligent in Shakespearean plays. Her masculinity appears in two ways. She manipulates Macbeth, demands of him brutal masculinity and thus leads him to the murder of Duncan. She also exercises cruel masculinity herself and thoroughly supervises the process of Duncan's murder. Macbeth's first crime, the murder of Duncan, leads to his ensuing crimes, beginning with killing two chamberlains first and then Banquo, and finally brings about his own tragic end. And the murder of Duncan results from Lady Macbeth's instigating Macbeth to do. So Macbeth's destruction results from Lady Macbeth's manipulation and especially from the influence of her masculinity. Lady Macbeth leads Macbeth to tragic end by her brutal masculinity, which is not the only one but one of its causes.

생리 활성 세라믹 분말이 첨가된 포장용 LDPE 필름의 새로운 에틸렌 평형 용해 모델

류동완,정재철,심승우,박찬영 전남대학교 촉매연구소 2000 觸媒硏究 論文集 Vol.21 No.-

This work investigates a new model that predicts the equilibrium solubility of gaseous component with polymer film. The new model regards the gas concentration in fluid phase as depressing factor in desorption from the surface of the film. The final mathematical form describes actual observation well with its second order form. The over all figure of the equilibrium solubility is sigmoid that one sees frequently in experiments. The only disadvantage of this model is the bulk concentration appears in denominator that induces the expression becomes infinite if the concentration is none. Insertion of one constant added to the concentration term in the denominator defeats this shortcoming. The following is the mathmatical form of the net rate of dissolution and the equilibrium solubility, respectively. □ Also, this paper presents the logical method obtaining the parameters from the experimental equilibrium data for the ethylene dissolution in LDPE film that had incorporated a ceramic powder with selective gas permeability. Finally, the dependency of the equilibrium parameters, KA, Cmax, the others to the weight fraction of the powder and appears to be linear and presented to follow : The parameters were calculated by soluble equilibrium constant, KA, maximum soluble concentration, Cmax and soluble rate constant, k+ for ethylene gas permeated through LDPE packaging films containing the ceramic powders 0∼6wt% at 20℃, the above model being applied. The values showed 1.45×106∼3.70×106[(㎤/mol)2], 2.30×10-3~2.92×10-3 [mo1/㎠sec]and 0.273∼0.405[㎤/mol sec], respectively.

현역군인 발생을 중심으로 본 2001년도 국내 삼일열 말라리아 발생 현황

류승호,이원자,김영아,채종일,박재원 대한감염학회 2002 감염 Vol.34 No.5