서울의 PPNG 발생 빈도(1991-1992)

김재홍,윤기범,박평원,김영진,전경민,김영태,김중환,곽호,구상완,송민석,유옥,지혜구,김동원,문상은,박영립,정승호,성범진,성순제,엄주용,황정열,이기홍,이주협,전태진 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1

The prevalence of PPNG among pretreated gonorrhea cases isolated at the STD clinic of Choong-Ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1991, 123 strains of N.gonorrhoeae were isolated, among which 58(47.1%) were PPNG. In 1992, 98 starains of N.gonorrhoeae were isolated, among which 51(52.0%) were PPNG. In all, 109(49.3%) strains were found to be PPNG among 221 strains isolated between 1991-1992. The prevalence of PPNG in Seoul showed increased tendency till 1989, thereafter, it has been stationary or slightly decreasing.

Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

전경대원에서 발생한 결핵의 임상 양상

문창기,박상준,조민구,김영중,김소연,김윤권,정준오,안석진,김은실,서승오,김지훈,최원제,이윤영,박형기,최규영,김현근 대한감염학회 2006 감염과 화학요법 Vol.38 No.6

Background: The purpose of this study was to document the incidence and clinical characteristics of patients with tuberculosis (TB) in combat and auxiliary police, living in a group, in Korea where the incidence rate of active TB in a general population is higher than in Western countries. Materials and Methods:We retrospectively reviewed the medical records of all tuberculosis patients diagnosed at National Police Hospital from January 2002 through December 2004. Results:In 2002-2004, a total of 156 cases of tuberculosis were identified with the mean (Standard deviation) age of 20.6 (±1.0) years. Of these, 134 (85.9%) patients were registered as new cases, 11 (7.1%) as relapse, 2 (1.3%) as failure, 5 (3.1%) as treatment-after-default cases whereas 4 (2.6 %) patients were not included in any categories. Average annual new TB rate and smear-positive TB rate were 86.5/10^(5) and 17.4/10^(5) person-years, respectively. In 12 of 31 smear-positive cases, time from onset of symptoms to diagnosis was more than 30 days. Two multidrug-resistance TB cases were identified and two suspected outbreak episodes of TB had occurred during 3 years. Conclusion:There was no statistically significant difference in the incidence rate of new cases of TB between the general population aged 20 to 29 years and combat and auxiliary police in Korea. 배경 : 국내 결핵의 발생은 감소 추세에 있으나 여전히 후진국형 발생양상을 보이고 있다. 특히 집단생활을 하는 젊은이들에서 발생하는 결핵의 유행이 간헐적으로 알려지고 있어 우려를 낳고 있다. 이에 저자들은 최근 전경대원의 결핵 발병 양상에 대해 알아보고자 본 연구를 시행하였다. 재료 및 방법 : 2002년 1월부터 2004년 12월까지 3년간 경찰병원에서 결핵 진료를 받은 전경대원의 의무기록을 후향적으로 분석하여 연도별 결핵 신환발생률, 도말양성결핵 신환율, 진단 지연, 집단발병, 다제내성결핵을 조사하였다. 결과 : 연도별 결핵 신환자 수(10만명당 발생추정치)는 2002년 50명(83.6명), 2003년 42명(83.7명), 2004년 42명(93.5명)이었고 도말양성 폐결핵 신환자 수는 2002년 10명(16.7명), 2003년 11명(21.9명), 2004년 6명(13.4명)이었다. 3년간 도말양성 폐결핵 환자는 31명이 있었고 그 중 12명은 호흡기 증상 발생 30일이 지나서 진단을 받았다. 다제내성 결핵은 2명이 있었으며 결핵의 집단발병이 의심되는 사례는 2차례 있었다. 결론 : 전경대원과 20-29세 연령군 일반인의 결핵 신환발생률, 도말양성 폐결핵 발생률의 통계적으로 유의한 차이는 없었다.

초등학교 열린학습공간 이용실태 조사연구

김승제 대한건축학회 2005 대한건축학회논문집 Vol.21 No.2

Since 1990, the plan to improve the backward educational environment which was partly influenced by economic development in Korea has started to construct modernized school buildings. Issuing open school theme from 1993, constructing modernized school buildings has spread over the whole country, But it is still in the situation that we do not clearly point out the advantages and disadvantages of open education. Behavioral studies to accommodate open education have been continually done by some researchers. But field surveys in these researches have been performed in the condition of not open architectural spaces but standard planning classroom. Moreover, we cannot yet see a research in which field survey is done in open planning space. Thus, the behavioral research in which case study is performed in open space classroom should be performed to evaluate the modernized school buildings that have been constructed so far. This research is aimed to suggest behavioral patterns of open education in open space in Jang-an elementary school in Seoul that was constructed in 1996 as a first example of modernized school. This research concludes 1) the present architectural condition of the school building in terms of field survey, 2) analysis of actual use conditions in open planning classroom, and 3) an attitude analysis of teacher.

순수 에탄올 경화요법을 통한 경부 및 견부 임파관종 치험 1 예

김동익,허승,이병붕,도영수,이순정,박제훈 대한혈관외과학회 1998 Vascular Specialist International Vol.14 No.2

Cystic lymphangioma is congenital anomaly originated from the lymphatic system. It occurs at head and neck area mainly. But its unusual location is possible. Treatment of cystic lymphangioma was surgical removal, but recently nonsurgical approach were studied, including sclerotherapy. Bleomycin, OK-432, and absolute ethanol were used as sclerotherapeutic agents. A 31 year-old female patient was treated by sclerotherapy with absolute ethanol for a cystic lymphangioma in neck and shoulder region. She had detected a mass in neck and shoulder region 8 years ago and had been done excisional surgery twice, 7 years and 4 years ago respectively. She had a 5 ×4×12 cm sized mass, that was recurred, in her neck and shoulder region. She had sclerotherapy twice in a 3-month interval. Its size decreased 2 cm in diameter from 12 cm.

정신병적 우울증의 신경심리학적 양상 : 비정신병적 우울증 및 정신분열병과의 비교 Comparison with Nonpsychotic Depression and Schizophrenia

김진세,유승호,정인과,제용진,김해열 대한생물치료정신의학회 1997 생물치료정신의학 Vol.3 No.1

본 연구는 24명의 비정신병적 우울증 환자, 25명의 정신병적 우울증 환자, 그리고 49명의 정신분열병 환자 총 98명을 대상으로 신경심리학적 양상을 비교하여, 정신병적 우울증이 비정신병적 우울증과는 다른 신경심리학적 이상이 있는지에 대하여 알아보았다. 신경심리학적 검사로써 숫자상징검사, 선로잇기검사 A형과 B형, 수지력검사, 시각지속수행검사, 공간기억검사 및 위스콘신 카드 분류검사 등을 시행하였다. 그 결과는 다음과 같다. 1) 숫자상징검사, 선로잇기검사 A형에서 비정신병적 우울증 환자군이 정신분열병 환자군보다 통계적으로 유의하게 높은 수행능력을 보였다. 2) 시각지속수행검사에서 비정신병적 우울증 환자군이 정신분열병 환자군보다 통계적으로 유의하게 높은 수행능력을 보였으며, 정신병적 우울증 환자군은 정신분열병 환자군과 유사하게 낮은 수행능력을 보였다. 3) 공간기억검사에서 정확한 반응의 비율은 정신분열병 환자군이 다른 두 군보다 통계적으로 유의하게 낮은 수행결과를 보였고, 정신병적 우울증 환자군은 비정신병적 우울증 환자군과 서로 비슷했다. 4) 위스콘신카드 분류검사에서 비정신병적 우울증 환자군은 정신병적 우울증 환자군과 정신분열병 환자군에 비해서 통계적으로 유의하게 높은 수행능력을 보였고, 정신병적 우울증 환자군과 정신분열병 환자군 사이에는 통계적으로 유의한 차이를 보이지 않았다. 결론적으로 정신병적 우울증의 신경심리학적 양상은 비정신병적 우울증과 다르며, 특히 전두엽의 기능부전을 의심할 수 있는 소견을 보였다. Objectives : A lot of studies have suggested evidences that psychotic depression is different from nonpsychotic depression in many aspects. Hence, the authors compared clinical variables and neuropsychological features of psychotic depression to those of non psychotic depression and schizophrenia in order to find out whether psychotic depression is classified by the different diagnostic category to nonpsychoticdepression. Methods : We examined the neuropsychological features of 24 nonpsychotic depression patients, 25 psychotic depression patients, and 49 schizophrenia patients. The neuropsychological tests that we made comprise digit symbol test, trail-making test A, trail-making test B, finger tapping test, continuous visual performance test, spatial memory test, and Wisconsin card sorting test. Results : 1) Mean score of digit symbol test and trail making test A in nonpsychotic depression group is statistically higher than in schizophrenia group. 2) Nonpsychotic depression group showed statistically higher accuracy than schizophrenia group and psychotic depression group showed similar result with schizophrenia group in continuous visual performance test. 3) Schizophrenia group showed statistically lower rates of accurate responses than the other two groups in spatial memory test. 4) Nonpsychotic depression group showed better performances than the other two groups and psychotic depression group showed no significant difference with schizophrenia in correct total score, error total score, and completed categories score for Wisconsin card sorting test. Conclusions : Above results show that frontal dysfunction might be appeared in psychotic depression. Therefore, we suggest that psychotic depression might be distinguished from nonpsychotic depression.

한국 마을숲 관리 개선방안 연구

김학범,장동수,이승제 한국전통조경학회 2003 한국전통조경학회지 Vol.21 No.4

This study was carried out to survey the state of growth, sustainable management, and improved plans of the village groves. The purpose of this study was to propose better methods of village grove management on 24 village groves of Kangwon and Kyungsang provinces. The main study field was classified into two parts. The one is cultural situation (historical backgrounds, user behaviour, development, land owners, managers, access, and parking) and the other is natural situation(growing condition, land condition, soil condition, and damage by blight and noxious insects) of Korean village groves. The data have been collected during the site survey. And then they were analyzed by frequency and cross tabulation analysis with SPSS 10 version. And they stand on roadsides, entrances of villages, riversides, and edge ways of the farmland. These groves are influenced by human actives, and vehicles. It diagnosed the current condition of the village groves with a field survey of the area. It analyzed natural condition(vegetation structure, land and soil, surrounding environment) of village groves and proposes better methods of facility, user, soil, plant, and tree management in order to improve natural conditions for them. The possibility of their sustenance can be determined from continuous management and program series, which involve recorded history, culture, importance of resources and ecological value etc.

진달래 꽃에 의한 Grayanotoxin 중독 3 례

김아진,김준식,신동운,백광제,한승백,이용주 대한응급의학회 2000 대한응급의학회지 Vol.11 No.3

Traditionally, the Rhododendron species has been used in gastrointestinal disorder or hypertension. Grayanotoxin exists in honey, flowers, pollen, and the nectar of the Rhododenron species. We experienced 3 cases of Grayanotoxin intoxication. The symptoms of intoxication were nausea, vomiting, hypotension, bradycardia, diplopia, dizziness, and chest discomfort. Generally, the treatment for Grayanotoxin intoxication is fluid resuscitation and injection of atropine sulfate. The patients who were intoxicated with Grayanotoxin were discharged without complication after supportive care.

초등학교 통폐합 과정에 관한 실태 조사연구

김승제 대한건축학회 2004 대한건축학회논문집 Vol.20 No.1

The opinion on merits and defects caused the merger and abolition of a school facilities have collected by a people and government since the unified-closed system was enforced. There is a problem that the data occurring in the middle of the unified-closed is not analyzed. Therefore, in this study the target is elementary schools in kyung-gi province which did the merger and abolition. First, this study is for analysing which condition influenced to make them. Second, it is for understanding the problems occurred in the process of the merger and abolition and presenting the direction of improvement. Third, it is for setting up the planned guide by classification of school facility for the merger and abolition which considers the previous problems sufficiently. The range of the study treats of general trend and analysis about the merger and abolition of elementary school. The core subject is to analyze the standard and classification of the merger and abolition of the object school by the policy of the merger and abolition and to compare the carried process of the case school.

수지상세포의 방사선 저항성에 대한 연구

김은실,김종순,이명호,범희승,민정준,정환정,김성민,허영준,송호천,이제중 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.3

목적 : 림프구와 비교되는 수지상 세포의 방사선 민감성을 보기 위하여 본 연구를 시행하였다. 대상 및 방법 : 말초혈액에서 분리한 T 림프구에 0 Gy, 10 Gy, 30 Gy의 방사선을 조사하고 4시간 후에 유세포 분석기를 이용하여 선량별 세포고사 빈도를 관찰하였다. 또한 조혈모세포에서 미성숙 및 성숙 수지상 세포를 단계적으로 분리 배양하여 각각 0 Gy, 10 Gy, 30 Gy, 100 Gy의 방사선을 조사하고 4시간, 24시간 그리고 48 시간 후에 선량별 세포고사 빈도를 관찰하였다. 사이토스핀(cytospin)슬라이드에 림프구와 미성숙 및 성숙 수지상세포를 3×104개 씩 분주하고 May Grunwald-Giemsa 염색한 후, 광학 현미경 하에서 각각의 세포군 당 100개의 세포에서 세포 면적당 핵의 면적 비를 측정하였다. 결과 : 림프구에서는 방사선조사 선량별로 세포고사 빈도가 유의하게 증가하였으나, 수지상 세포에서는 그 분화정도나 방사선조사 선량에 따른 세포고사의 빈도차이가 없었다. 또한 수지상 세포는 방사선선량과 관계없이 용량에 의존적으로 강력한 T-세포 자극능을 보였다. 림프구의 세포에 대한 핵의 면적 비는 미성숙 및 성숙 수지상세포의 세포에 대한 핵의 면적 비보다 현저히 큰 반면, 두 가지 수지상세포간에 유의한 차이는 없었다. 결론 : 수지상세포는 그 분화도와 상관없이 림프구에 비하여 방사선 저항성을 나타내었고, 이는 세포의 형태적 차이에 따른 표적의 크기와 관련이 있을 것으로 생각되며, 향후 분자 생물학적인 연구의 기초 자료로 활용할 수 있을 것으로 생각된다. Purpose : To evaluate radiation sensitivity of dendritic cells in comparison with lymphocytes. Materials and methods : T lymphocytes captured from peripheral blood were irradiated by 0 Gy, 10 Gy, 30Gy. Apoptosis was measured by flowcytometry for staining of Annexin V 4 hours after irradiation. Immature and mature dendritic cells processed from blood hematopoietic stern cell were irradiated by 0 Gy, 10 Gy, 30 Gy, 100 Gy respectively and apoptosis was measured by flowcytometry with time difference as 4h, 24h and 48h after irradiation. Morphometric analysis by percent nucleus was measured in three cell groups, also. Results : Lymphocytes showed radiation sensitivity by increasing apoptotic fraction according to radiation dose. However, both mature and immature dendritic cells showed consistent fraction of apoptosis in spite of increasing radiation dose. Percent nucleus ratio is significantly higher in lymphocytes than that of mature or immature dendritic cells. Stimulation of T-cell by dendritic cells was not changed after irradiation. Conclusion : Dendritic cells showed radioresistance which was associated with small size of nucleus in comparison with lymphocytes and this result would be used as a basal data of radio-labelling for the cellular trafficking studies in nuclear medicine fields.