신경세포성장인자로서 척수운동신경세포의 손상에 미치는 한약재의 약류별 효능 및 기전에 관한 연구

鄭遇悅,朴承澤,成彊慶,李星根 대한동의병리학회 1998 동의생리병리학회지 Vol.12 No.2

산소자유기가 흰쥐의 배양 운동신경세포에 미치는 세포독성에 대한 기전을 규명하기 위하여 여러 농도의 산소자유기를 배양 신경세포에 처리한 후 산소자유기의 신경독성 효과를 분석하였으며 또한 산소자유기에 의하여 유발된 신경독성에 대한 한약재중 보혈약류와 보기약류의 대표적인 처방인 사군자탕과 사물탕의 신경세포독성의 방어효과를 MTT assay법에 의하여 조사하여 다음과 같은 결론을 얻었다. Xanthine oxidase(XO)와 hypoxanthine(HX)은 처리한 농도에 비례하여 배양 신경세포의 생존율을 현저하게 감소시켰으며, 또한 사물탕과 사군자탕이 XO/HX의 독성효과를 유의있게 방어하였다. 이상의 결과로부터 산소자유기는 배양 소의 희소돌기아교세포에 독성을 나타냈으며 사군자탕과 사물탕등의 한약추출물이 산소자유기의 독성을 방어하는데 효과적인 것으로 나타났다. In order to elucidate the neuroprotective effect of Sagunjatang and Samultang on oxidant-induced neurotoxicity in cultured rat spinal motor neurons, the colorimetric assay such as MTT assay was performed and light microscopic study was also carried out after cultured spinal motor neurons from neonatal rat were treated with the medium containing various concentrations of xanthine oxidase(XO) and hypoxanthine(HX) for 3 hours. In addition, protective effect of herb extracts on the neurotoxicity induced by oxygen radicals was examined in these cultures. The results were as follows : Cell viability of cultured rat spinal motor neurons which were exposed to various concentrations of 30mU/ml XO and 0.1mM HX for 3 hours, was decreased in a dose-dependent manner. MTT50 value was 30mU/ml XO and 0.1mM HX after motor neurons were incubated with various concentrations of XO/HX for 3 hours. Cultured spinal motor neurons showed degenerative changes such as the decrease of cell number and loss of cell processes when neurons were cultured in the medium containing 30mU/ml X0 and 0.1mM HX for 3 hours. Oxygen radicals were toxic on cultured rat spinal motor neurons by the decrease of cell viability. Herb extracts, Sagunjatang and Samultang prevented the oxidant-induced neurotoxicity significantly after spinal motor neurons were preincubated with herb extractions for 2 hours before exposure of oxygen radicals. These results suggest that oxygen radicals have neurotoxic effect on cultured spinal motor neurons derived from neonatal rat, and selective herb extracts such as Sagunjatang and Samultang are very effective in protecting the neurotoxicity induced by oxygen radicals.

알코올의 진정 효과와 일부 인지 기능에 미치는 영향

호정일,왕성근 대한생물치료정신의학회 2003 생물치료정신의학 Vol.9 No.1

Objectives : The purpose of this study was to evalute the effect of alcohol on sedation and some cognitive function(eg,daytime sleepiness, fatigue, and mood measured by multiple sleep latency test(MSLT) and visual analogue scale(VAS), vigilance, attention and short-term memory measured by Vienna Test System). Methods : 15 healthy, normal young college students participated in this study. Participating subjects were in good physical and psychological health and were asymptomatic as to sleep/wake complaints. Subjects visited our laboratory by 08 : 00 at the first day, and they had baseline MSLT and Test(at 9;00, 11;00, 14:00, and 16:00).Each time, the subjects completed VAS for sleepiness, fatigue and mood before they underwent an Vienna Test to evaluate their vigilance, attention and short-term memory. On the second day, subjects had 0.5g/kg of alcohol, the third day, subject had 1.0g/kg of alcohol, and repeated the same process as those of the first. Results : 0.5g/kg of alcohol reduced sleep latency after 1 hour, and 1.0g/kg of alcohol reduced sleep latency until 5 hours after administration significantly. 0.5g/kg of alcohol induced daytime sleepiness 1 hours after administration, and 1.0g/kg of alcohol induced daytime sleepiness and fatigue until 3 hours after administration, but there were no effects on mood. Vigilance was decreased significantly 1 hour after 0.5g/kg of alcohol administration, and attention and short-term memory were not changed significantly after alcohol administration. After alcohol adminstration(0.5-1.0g/kg), there were significant positive correlation between daytime sleepiness and fatigue, and fatigue and mood. Conclusion : It can be concluded that alcohol decreases sleep latency and induces daytime sleepiness and fatigue, and decreases vigilance in normal subjects.

손상정도계수와 외상치를 이용한 외상환자의 분석

송근정,장문준,김성중,조광현,김인병,최성욱,이한식 대한응급의학회 1993 대한응급의학회지 Vol.4 No.2

Trauma is one of the most common causes of death. Recently, injury severity score(ISS) is widely used to evaluate and classify the patients with multiple injury. Trauma score(TS) was developed as a means of providing rapid accurate assessment of the injured patient. Authors conducted a review of trauma patients who visited the emergency department of YongDong Severance Hospital from September 1992 to August 1993. ISS and TS were collected from comprehensive initial evaluation. The results were as follows : 1) The subjects were 10.2%(2,666 patients) of total 26,202 emergency patients. 2) Male to female ratio was 2.1 : 1. The age distribution was highest under 10 year(24.9%). The mean ISS was 3.5, and the mean ISS increased with ago. 3. Analysis of injury mechanism showed high incidence of traffic accident(35.3%), blunt trauma 25.7%, fall 24.2%, stabbing 10.7%, and others 4.3% in order. 4. The 8.0% of patients were admitted after initial management and their mean Iss was 12.6 mean TS was 15.5. 5. Among admitted traffic accident patients, the mean ISS of passenger T.A., pedestrian T.A. and driver T.A. were 19.0 ±9.9, 14.4 ±10.6, and 11.3 ±7.7 respectively. 6. Among 42 mortality cases, 25 patients were D.O.A. and 17 were expired. The mean ISS of D.O.A. Patients was 30.0; the mean Iss and mean Iss and mean TS of expired patients were 28.3 and 11.8 respectively. 7. The most common cause of death was head trauma(66.7%). 8. By comparing the ISS and TS of admitted and expired patients, mortality occurred above 16 point of ISS. The mortality increased with increasing ISS, but it was influenced by the trauma site and the severity of the particular injury site. Mortality also occurred below 15 point of TS, the mortality increased inversely with TS, and the mortality with TS, and the mortality was high below 13 point (above 40%).

국내에서 분리된 Extended-Spectrum β-Lactamase 생성 Escherichia coli와 Klebsiella pneumoniae의 빈도 및 유형

홍성근,김선주,정성훈,장철훈,조성란,안지영,신종희,이혜수,송원근,어영,염종화,용동은,이경원,정윤섭 대한임상미샐물학회 2003 Annals of clinical microbiology Vol.6 No.2

정상인에서 부분적 수면박탈이 수면구조에 미치는 영향

왕성근,이정규,이충숙,최하석,유남재,정인형,이선우 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.2

To identify the effects of partial sleep deprivation on the sleep structure, the authors performed polysomnographic study for 9 healthy young adults. The subjects were college students, ranging in age from 20 to 25 years, without personal past or present histories of medical, neurological, or psychiatric illness and suffering from current sleep disturbance or parasomomnias. Sleep records were analyzed according to the criteria of Rechtschaffen and Kales' manual and tried paired t-test. The results were summarized as follows. 1. As for sleep parameters, total sleep time(TST), REM latency and REM duration were not changed significantly during deprivation period and recovery period compared with baseline. Sleep efficiency increased significantly during deprivation period and recovery period compaired with baseline, and sleep latency and WASO were shortened significantly during deprivation period and recovery period compared with baseline. 2. As for sleep structure, 1) The absolute value(minutes) of stage 1 decreased significantly during deprivation period and first recovery night compared with baseline, and the relative value(percentage) of stage 1 decreased significantly during deprivation period and recovery period compared with baseline. 2) The absolute value(minutes) of stage 2 decreased significantly during deprivation period compared with baseline, and the relative value(percentage) of stage 2 decreased significantly during deprivation period and first recovery night compared with baseline. 3) The absolute value(minutes) of slow wave sleep were not changed significantly during deprivation period and recovery period compared with baseline, and the relative value(percentage) of slow wave sleep increased significantly during recovery period compared with baseline. 4) The absolute value(minutes) of REM sleep decreased significantly during deprivation period compared with baseline, and the relative value(percentage) of REM sleep decreased significantly in the first deprivation night but, were not changed significantly during recovery period compared with baseline.

충수염의 임상적 고찰과 초음파의 유용성

박정배,양혁준,김형수,황성연,박철완,이근 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

Appendicitis is the most common acute abdomen in Emergency Medical Center & outpatient department. But sometimes, it is difficult for us to diagnose because of clinically variable symptoms. We reviewed the ultrasonographic findings and analyse 521 cases of appendectomy patients about its distribution of sex and age, seasonal prevalence, clinical symptom and signs, laboratory findings, duration from onset of symptom to arrival at hospital, duration from arrival at hospital to operation, vital signs, operative results, and associated diseases, which were treated at the department of General Surgery & Emergency Medical Center in Chung Ang Gil Hospital from July, 1994 to June, 1995. The results were as follows : 1. The most common age group was second decade. And male to female ratio was 1.43:1. Seasonal variation was not significant. 2. Of 521 cases, 141 cases(27.1%) visited the hospital within 24 hours from the onset of symptom, and 259 cases(49.7%) visited between 24 to 48 hours. 3. In duration from arrival to operation, the cases within 6 hours were 263 (50.5%), the cases between 6 and 12 hours were 131(25..1%). 4. In systolic blood pressure, cases below 90mmHg were 19(3.6%), cases above 160mmHg were 23(4.4%). Below 60mmHg diastolic pressure were 55 cases(10.6%), cases above 110mmHg were 9(1.7%). In pulse rate, above 90 rate/min were 171(32.8%). 5. On admission leukocytosis was found in 413 cases(79.3%) and 342 cases of them were pathologic appendicitis. 6. Ultrasoundgraphy was performed in the 87 cases, but 23 cases were suggested appendicitis. Among 23 cases, 22(95.7%) revealed pathologic appendicitis at ooperation and labortatory examination. Of the 64 cases which were normal on ultrasonography, only 2 cases(3.1%) were proved to be appendicitis. 7. The results of 496 operations without ultrasonography were appendicitis in 390 cases(78.6%), mesenteric lymphadenopathy 36(7.3%), pelvic inflammatory disease 28(5.6%), fecalith 15(3.0%), terminal ileitis 8(1.6%), diverticulitis 7(1.4%), urinary tract stones 5(1.0%), cecal cancer 2 cases, right ovarian tumor 2 cases, tubo-ovarian abscess 2 cases, grater omentum internal hernia 1 case. Our results showed that the sensitivity of ultrasonohraphy was 95.8% and the specificity 98.4% in the diagnosis of appendicitis, It suggests that the ultrasonography is very helpful in the diagnosis & differential diagnosis of acute appeendiditis.

국내 카드뮴 취급 사업장의 카드뮴 폭로 실태 평가

강성규,홍정표,김기웅,장재연,정호근,정규철 大韓産業醫學會 1994 대한직업환경의학회지 Vol.6 No.2

Twenty-four factories registered at the Ministry of Labor as factories using or producing cadmium in 1991 were investigated to know the present state of occupational exposure to cadmium in 1992. Nine factories among 24 stopped using or changed to other substances and 1 factory didn't produce any more, but delivered it to other factories through imports. Therefore, only 14 factories were producing or using cadmium. But, alloying materials were supplied to about 20 and more factories, however, they couldn't be investigated because of limited information. Three factories had less than 50 workers were 3,6 had 50-300 and 5 had more than 300. About 167 workers were exposed to cadmium. According to the classification by process, there were 4 alloy processes, 2 refinary, 2 elctroplating, 2 dry cell making, 1 industrial battery making, 1 duplicator drum making, 1 recycling of battery and 1 copper refinary. 8 factories used metal cadmium, 3 cadmium oxide, 2 cadmium sulfide, and 1 cadmium hyroxide. One factory was importing cadmium stearates only and supplying them to the PVC making factories. The amount of metal cadmium producing in 2 refinary factories was about 50-65 ton per month. The other cadmium compounds were imported. According to exposure level of cadmium air of the 10 factories examined, each factory could be divided into high, moderate and low exposure groups. 1 battery recycling factory and 3 alloy factories were in the high exposure group and their mean air concentrations were 0.06679, 0.05481, 0.02492, and 0.003㎎/㎥, respectively. Refinary and industrial battery making factories were in the middle exposure group and their mean air concentrations were 0.00773 and 0.00613 ㎎/㎥, respectively. Finally, low exposure group were copper smelting, dry cell case making, duplicator drum making and electroplating factory and their mean air concentrations were 0.00096, 0.00071, 0.00057 and 0.00054 ㎎/㎥ respectively.

의료용 조합자극기(HAPPY DREAM HM-206DB) 적용이 동결견 환자의 통증과 관절가동범위에 미치는 영향

홍성찬,김영빈,박병근,김유성,이성진,정동혁 원광대학교 체력과학연구소 2008 體力科學硏究 Vol.30 No.1

The purpose of this study is to find out the effectiveness of the HAPPY DREAM HM-206DB applied to the patients with Frozen Shoulder by measuring, assessing and analyzing the changes in pain and range of motion(ROM). Surveyed from Mar. 2007 to Dec. 2007 were 10 patients suffering from Frozen Shoulder. After HAPPY DREAM HM-206DB, the pain were measured and analyzed by means of MPQWL, VAS-I and VAS-U, while ROM was measured and analyzed by using Goniometer. The major findings from this study are as follows; 1. There was significant decrease in the MPQWL after HAPPY DREAM HM-206DB applied(p<.001). 2. There was significant decrease in the VAS-I(intensity of pain) after HAPPY DREAM HM-206DB applied(p<.001). 3. There was significant decrease in the VAS-U(unpleasantness of pain) after HAPPY DREAM HM-206DB applied(p<.001). 4. There appeared, however, significant increase in ROM(abduction, external and internal rotation) after HAPPY DREAM HM-206DB applied(p<.001). From the above outcomes, it was revealed that HAPPY DREAM HM-206DB application has proven to reduce the pains, exercising huge influence to increase ROM. It is, therefore, suggested to continue and expand the study on the cure of Frozen Shoulder and to motivate patients, rehabilitation specialists, and health management specialists etc. to consider HAPPY DREAM HM-206DB application as safest and most efficient pain remedy.

Frozen Shoulder에 있어서 마사지의 치료적 접근

홍성찬,김영빈,박병근,정동혁,이정윤,이동헌 圓光大學校 附設 體力科學硏究所 2003 體力科學硏究 Vol.26 No.1

Painful stiffness of the shoulder is an ill-defined clinical entity that is difficult to assess and delicate to treat. The nomenclature used is broad and includes terms such as frozen shoulder, adhesive capsulitis, focal algodystrophy, stiff shoulder, contracted shoulder, and others. Apart from its idiopathic form, the disease can be initiated by trauma, infection, tumour, radiation, systemic and local metabolic disturbances. Pathoanatomically, the common denominator is an inflammatory vascular proliferation followed by thickening, scarring, and retraction of the joint capsule. The inflammatory process often starts at the rotator interval and may extend to the subacromial space. Clinical diagnosis is based on history and physical examination. Generally the onset of pain precedes the perception of a reduced range of motion by weeks or months. In early stages of the disease, the inflammatory type of pain dominates, i.e., the patient's main complaint ist pain at night. In the later stage, range of motion gradually decreases. Patients do not often complain about reduced motion, probably because of its slow onset. The purpose of this study is to find out the effectiveness of the massage applied to the patients with Frozen Shoulder by measuring, assessing and analyzing the changes in intensity and unpleasantness of pain and range of motion(ROM) before and after massage. Surveyed from Jan. 2001 to December 2002 were 104 patients suffering from Frozen Shoulder. In the therapeutic massage program, such methods as Effleurage, Petrissage and Deep transverse friction were selected as they are among traditional massage treatments frequently used for joints with pain and restricted ROM. Effleurage and Petrissage were applied for 20 minutes in total before and after Deep Transverse Friction. After massage program, the intensity and unpleasantness of pain were measured and analyzed by means of VAS(visual analogue scale)-I and VAS-U, while ROM was measured and analyzed by using Goniometer. The major findings from this study are as follows; 1. The surveyed patients range from 28 to 75 in age, with highest numbers of 44(42.31%) registered in the fifties and next ones of 24(23.08%) in the forties. Divided by sex, 59 are women and 45 are men totalling 104 with average age of 51.4. 2. There was significant decrease in the intensity of pain and unpleasantness of pain after massage(p<.05). 3. There appeared, however, significant increase in ROM after massage(p<.05). 4. From the analysis into chronological changes in ROM before and after massage with ANOVA, it became evident that the longer the period of treatment was, the higher the ROM increases drastically, while significant difference was shown in Abduction and in External and Internal Rotation(p<.05). Summed up, it can be generally concluded that massage is an effective treatment to rid the patients with Frozen Shoulder of pains safely and promptly. This study was conducted to determine more accurate impact of massage. From the above outcomes, it was revealed that massage has proven to reduce the pains, exercising huge influence to increase ROM. It is, therefore, suggested to continue and expand the study on the cure of Frozen Shoulder and to motivate patients, orthopedists, physiatrists, physiotherapists, and sports massagists etc. to consider massage as safest and most efficient pain remedy.

한국인 성인에서 Fm θ의 출현 양상에 관한 연구

도정수,신석철,왕성근 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.2

The distinctive theta rhythm that appears at the frontal midline during the performance of mental tasks has been designated as frontal midline theta(Fmθ). Author tried to confirm the appearance pattern of Fmθ in Koean young adults, and investigated the relations among the appearance rates of Fmθ, sex, kinds of mental tasks, characteristics of personality trait, impulsiveness, temperament, and anxiety level. The subjects were 42 healthy male and female students(28 male, 14 female) aged 23 to 25(average age 23.9±0.6). The results were summarized as follows. 1. The individual variation of the appearance and appearance rates of Fmθ were large, and the appearance rates of Fmθ under Uchida-Kraepelin test were ranged 28.6~31.0%, and under reaction time were ranged 4.8%~21.4%. 2. The appearance rates of Fmθ tend to increase under continuous mental task than simlpe concentration. 3. There were no sexual difference in the apearance rate of Fmθ. 4. In the aspect of the anxiety level, the appearance rate of Fmθ seems to bo increased when the person's level of anxiety is low, the excitement strength is high, and less impulsive. 5. In the aspect of the personality, the appearance rate of Fmθ seems to be increased when the person's personality is introvert, the excitement strength is high, and the motility of temperament is high.