암모니아 및 아질산성 질소 산화세균의 분리 및 특성

이용석,유주순,정수열,박춘수,최용락 한국농화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.1

본 연구는 폐수 중의 질소 제거를 위한 생물학적 처리용 미생물 개발을 위한 목적으로 질소의 산화 능력이 뛰어난 균주를 분리하였다. 분리된 세균 중에서 질소 산화능과 생육 속도가 뛰어난 CH-N 균주를 선별하였으며, 생리, 생화학적인 특성 조사에 의해 Bacillus sp로 추정되어 Bacillus sp. CH-N이라 명명하였다. 분리 균주는 0.5% glucose가 포함된 초기 pH가 7.0인 암모니아 및 아질산성 질소 함유 배지에서 30시간 배양 후 가가 85%와 90%의 암모니아성과 아질산성 질소가 단시간에 질소의 감소율을 나타내었다. 폐수 및 생활하수에 분리 균주를 이용한 결과, 수질 속의 암모니아성 질소가 단시간에 크게 감소시키는 효과를 확인하였다. 균주를 고정시킨 담체의 질소산화 효과를 시험하고자 Bacillus sp. CH-N을 고정시킨 세라믹 담체를 이용한 결과, 배양 2일 후에는 암모니아성 질소가 전부 제거되었다. In order to improve the system for biological nitrogen oxidizing process in sewage and wastewater, a bacterium having high abilities to oxidize of nitrogen was isolated from wastewater and polluted soils. The strain was identified to Bacillus sp. CH-N, based on the physiological and biochemical properties. Characteristics and oxidizing ability of both ammonia and nitrite were examined for the strain, Bacillus sp. CH-N. The strain showed the oxidizing rate about 80% to 90% on the sewage and wastewater after 48 h culture. The nitrogen oxidizing rate was increased in proportion to the initial concentration of glucose. The microorganism, Bacillus sp. CH-N cell immobilized on ceramic carrier were evaluated for the oxidation of ammonia in culture media.

Airborne Nicotine Concentrations in the Workplaces of Tobacco Farmers

Yoo, Seok-Ju,Park, Sung-Jun,Kim, Byoung-Seok,Lee, Kwan,Lim, Hyun-Sul,Kim, Jik-Su,Kim, In-Shik The Korean Society for Preventive Medicine 2014 Journal of Preventive Medicine and Public Health Vol.47 No.3

Objectives: Nicotine is a natural alkaloid and insecticide in tobacco leaves. Green tobacco sickness (GTS) is known as a disease of acute nicotine intoxication among tobacco farmers. Until now, GTS has been recognized globally as a disease that results from nicotine absorption through the skin. However, we assumed that GTS might also result from nicotine inhalation as well as absorption. We aimed to measure the airborne nicotine concentrations in various work environments of Korean tobacco farmers. Methods: We measured the nicotine concentrations in the tobacco fields, private curing barns, and joint curing barns of farmers from July to October 2010. All sampling and analyses of airborne nicotine were conducted according to the National Institute for Occupational Safety and Health manual of analytic methods. Results: The airborne nicotine concentrations (geometric mean [geometric standard deviation]) in the tobacco field were $83.4mg/m^3$ (1.2) in the upper region and $93.3mg/m^3$(1.2) in the lower region. In addition, the nicotine concentration by personal sampling was $150.1mg/m^3$. Similarly, the nicotine concentrations in the private curing barn, workers in curing barns, the front yard of the curing barn, and in the joint curing barn were $323.7mg/m^3$(2.0), $121.0mg/m^3$(1.5), $73.7mg/m^3$(1.7), and $610.3mg/m^3$(1.0), respectively. Conclusions: The nicotine concentration in the workplaces of tobacco farmers was very high. Future studies should measure the environmental concentration of nicotine that is inhaled by tobacco farmers.

Epidemiological Investigation of an Outbreak of Salmonellosis in Gyeongju, Korea

Yoo, Seok-Ju,Lim, Hyun-Sul,Lee, Kwan The Korean Society for Preventive Medicine 2014 Journal of Preventive Medicine and Public Health Vol.47 No.3

Objectives: A salmonellosis outbreak occurred within a community of Gyeongju residents who ingested catered food from a wedding in June 2009. We aimed to epidemiologically investigate the probable vehicle of the infection. Methods: We conducted a retrospective cohort study on 34 local residents who ingested the wedding food. Results: Among the 34 residents, 31 (91.2%) reported symptoms of infection after eating the food. Among all of the wedding foods, pan-fried foods were highly associated with the diarrheal attack rate. On bacteriological examination, Salmonella species were detected in the pan-fried foods among the leftover foods and in 17 of the 31 stool specimens from the cases. There were five different types of pan-fried foods, but the onset of symptoms was independent of the ingredients used. We found that the pan-fried food was prepared at a food store in Seoul and that eggs were a common ingredient. Conclusions: The major cause of the salmonellosis in this population was presumed to be the pan-fried food prepared with contaminated eggs. These food items might have been partially undercooked because of their irregular shape, which allowed the Salmonella species to survive and multiply before ingestion.

Analysis of the Change of Health Status Among the Republic of Korea Air Force Soldiers During Military Service

Yoo, Seok-Ju,Park, Won-Ju,Lee, Kwan,Lim, Hyun-Sul,Kim, Hyun-Jin,Lee, Suk-Ho The Korean Society for Preventive Medicine 2014 Journal of Preventive Medicine and Public Health Vol.47 No.4

Objectives: The aim of this study was to investigate whether the health status of Republic of Korea Air Force (ROKAF) soldiers changed after one year of military service. Methods: We selected 483 ROKAF soldiers from the 11 749 recruits who participated in the 2011 physical examination. The selected soldiers underwent another physical examination in 2012 for advancement to senior airman. Data from 2011 and 2012 were merged. To collect data on lifestyle, a questionnaire was sent to all included subjects via the military intranet e-mail service. Results: The percentage of recruits with an abnormal alanine transaminase level (normal range <40 IU/L) decreased from recruitment (13.7%) to the following year (2.7%). Moreover, the percentage of obese soldiers (body mass index ${\geq}25kg/m^2$) decreased from recruitment (20.5%) to the following year (10.4%). There was a significant change in mean duration of exercise carried out each day before ($0.8{\pm}1.3$ hours) and after ($1.0{\pm}0.7$ hours) joining the ROKAF service. Conclusions: These ROKAF soldiers were generally in good health before and after joining the armed service. After one year of military service, the health status of most soldiers improved, especially with respect to body mass index and alanine transaminase level.

Current Status of Ultrasonography Examination Operated by National Cancer Surveillance Program for Hepatocellular Carcinoma: Multi-Center Large-Scale Research

( Jeong-ju Yoo ),( Sun Hong Yoo ),( Jung Hyun Kwon ),( Han-ah Lee ),( Yeon Seok Seo ),( Young Kul Jung ),( Hyung Joon Yim ),( Do Seon Song ),( Seong Hee Kang ),( Moon Young Kim ),( Young-hwan Ahn ),( 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1

Aims: Abdominal ultrasonography (USG) is recommended as a surveillance test for the high-risk group of hepatocellular carcinoma (HCC). However, there is lack of information about how USG examination is actually performed in clinical practice, and how the quality control related to USG examination is managed. We investigated the incidence of HCC by a reference point of 6 months after USG was conducted. Methods: We collected data of surveillance USG from high-risk group of HCC (liver cirrhosis, or chronic hepatitis B or C over 40 years) group during 2017 in 8 tertiary hospitals. To determine the accuracy of the ultrasound examination itself, patients who had a prior history of HCC or who had CT or MRI scan within 6 months of ultrasound were excluded. If ultrasound was performed more than twice during 2017, the results of the first test were used. Results: In 2017, 45 experienced hepatologists or radiologists performed 8,512 ultrasounds. Mean age was 53.5 ± 11.0 years and, 4817 (56.6%) were males. Chronic hepatitis B was the most common indication (6319, 74.2%), and 2429 patients (28.5%) had liver cirrhosis. Doctors had 15.0 ± 8.3 years of experience, and proportion of hepatologists was higher than that of radiologists (61.4% vs. 38.6%). The time taken for the ultrasound scan was 4.1 ± 3.3 minutes excluding the preparation time. The detection rate of HCC through surveillance USG was 0.3% (29 patients). During 27 months of follow-up, a total of 60 patients (0.7%) developed new HCC. Of these, 15 patients (0.2%) were diagnosed with HCC within 6 months of the surveillance USG, and 45 patients (0.5%) were diagnosed after 6 months of the surveillance test. Conclusions: This is the first study on the current status of USG examination as a surveillance modality for HCC. It is necessary to develop quality indicator and quality assessment of USG to increase the detection rate of HCC.

Long-term prognosis and the need for histologic assessment of chronic hepatitis B in the serological immune-tolerant phase

Jeong-Ju Yoo,Soo Young Park,Ji Eun Moon,Yu Rim Lee,Han Ah Lee,Jieun Lee,Young Seok Kim,Yeon Seok Seo,Sang Gyune Kim 대한간학회 2023 Clinical and Molecular Hepatology(대한간학회지) Vol.29 No.2

Background/Aims: The histologic status of the immune-tolerant (IT) phase of chronic hepatitis B relative to long-term outcomes is unclear. This study aimed to discover how the serological criteria currently in use correspond to histologic criteria in determining the IT phase and indication for liver biopsy. Methods: Patients in the serological IT phase determined by positive hepatitis B e antigen, hepatitis B virus (HBV) DNA ≥106 IU/mL, and normal or minimally elevated alanine aminotransferase (ALT) ≤60 IU/L, who underwent liver biopsy at three different hospitals were included. The distribution of the histologic IT phase, defined as fibrosis of stage 1 or less and inflammation of grade 1 or less, was compared with that of the serological IT phase. The risk factors for the incidence of liver-related events, such as hepatocellular carcinoma, liver cirrhosis, liver transplantation, and death, were also analyzed. Results: Eighty-two (31.7%) out of 259 clinically suspected IT phase patients belonged to the histologic IT phase. Age over 35, high AST, and low albumin were useful for ruling out the histologic IT phase. Risk factors predicting liver-related events were age and significant fibrosis stage. There was no significant difference in the proportion of histologic IT phase and clinical prognosis between normal ALT and mildly elevated ALT groups. However, even in patients with normal ALT, age was an important factor in predicting the presence of the histologic IT phase. Conclusions: A significant number of patients who belonged to the serological IT phase were not in the histologic IT phase. Patients over 35 years and those with high AST, low albumin, and low HBV DNA levels were more likely to experience poor long-term clinical outcomes. Therefore, additional histologic assessment should be considered.

이중공기막하우스와 일반 플라스틱하우스의 에너지절감 효과 비교

유주열(Ju Youl Yoo),심상연(Sang Youn Sim),이승주(Seung Ju Lee),고석남(Seok Nam Ko),신동창(Dong Chang Shin),김영식(Young Shik Kim) 한국원예학회 2013 한국원예학회 학술발표요지 Vol.2013 No.10

Relationship between Timing of Endoscopy and Mortality in Cirrhotic Patient with Variceal Bleeding

( Jeong-ju Yoo ),( Young Chang ),( Eun Ju Cho ),( Sang Gyune Kim ),( Young Seok Kim ),( Yun Bin Lee ),( Jeong-hoon Lee ),( Su Jong Yu ),( Yoon Jun Kim ),( Jung-hwan Yoon ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: The optimal timing of emergency endoscopy in patients with variceal bleeding is remains unclear. Most guidelines recommend performing endoscopic evaluation and treatment within 12 hours after patient’s arrival, but there are few related studies. The aim of this study was to examine the association between timing of endoscopy and mortality of esophageal variceal bleeding in cirrhotic patients. Methods: This retrospective study included 274 of consecutive patients admitted to two tertiary hospitals in Korea due to esophageal variceal bleeding. Using descriptive statistics and Kaplan-Meier survival analyses, we analyzed the association between the timing of endoscopy and patients’ mortality. We adjusted confounding factors and balanced the baseline characteristics of the subjected patients using Cox proportional hazards model and inverse probability weighting (IPW) method. Results: A total of 173 patients received endoscopy within 12 hours after admission and 101 patients, after 12 hours. Endoscopy was performed after a median of 7.6 hours (interquartile range [IQR], 2.9-16.5) after admission. The median overall survival (OS) was 8.2 months (IQR, 1.4-25.9) months, and in-hospital mortality was 8.0%. Performing endoscopy within 12 hours was associated with longer OS than performing after 12 hours (33.2 vs. 23.5 months, log-rank P=0.01). Early endoscopy was independently associated with longer OS after adjusting presence of hepatocellular carcinoma, MELD score, and Glasgow-Blatchford score (adjusted hazard ratio [aHR] 1.61, 95% confidence interval [CI]=1.05-2.46, P=0.03). Also, after balancing baseline characteristics using IPW, endoscopy within 12 hours group consistently showed longer OS than endoscopy after 12 hours group (aHR 2.17, 95% CI=1.56-3.03; P=0.03). However, outcomes were not significantly different between the urgent (within 6 hours) and early endoscopy groups. Conclusions: Timing of endoscopy is associated with mortality in patients with esophageal variceal bleeding. It is important to perform emergency endoscopy within 12 hours, especially for high-risk patients.

무선전력전송과 IH 기술융합을 위한 고효율 중전력 시스템

유주승(Ju-Seung Yoo),이종주(Jong-Ju Lee),송두익(Doo-Ik Song),민병덕(Byung-Duk Min),조정구(Jung-Gu Cho),이성훈(Seong-Hun Lee),염정석(Jung-Seok Yeom),장원호(Won-Ho Jang) 대한전기학회 2015 대한전기학회 학술대회 논문집 Vol.2015 No.7

소 사육업자의 인수공통감염병 관련 요인

유석주 ( Seok-ju Yoo ),임현술 ( Hyun-sul Lim ),이관 ( Kwan Lee ) 대한보건협회 2016 대한보건연구 Vol.42 No.3

연구목적 : 이번 연구는 국내 소 사육업자들의 인수공통감염병과 관련된 요인을 파악하고 인적 특성과의 관련성을 분석하여맞춤형 인수공통감염병 예방을 위한 자료로 활용하고자 진행하였다. 연구방법 : 2015년 2월부터 7월까지 각종 단체 행사에 참여한 전국의 소 사육업자 중 무작위로 추출하여 선정한 후, 연구참여에 대하여 자발적 동의를 한 407명을 연구대상으로 하였다. 소 사육업자들의 단체 행사 일정을 미리 파악한 후, 설문요원이 직접 행사장을 방문하여 일 대 일 면접조사 방식으로 설문조사를 실시하였다. 소 사육업자들의 인수공통감염병과 관련된요인들을 작업과 관련된 것과 작업 이외의 식습관 혹은 인지도와 관련된 것으로 분류하여 이를 각각 소 사육업자들의 인적특성에 따라서 단변량 분석을 실시하였다. 단변량 분석에서 인수공통감염병 관련 요인에 대하여 유의한 차이가 있었던 인적특성 변수들은 서로 보정하여 이분형 로지스틱 회귀 분석을 실시하였고, 최종적으로 인수공통감염병 관련 요인 노출 유무에영향을 주는 변수들을 선정하여 교차비(odds ratio, OR)와 95% 신뢰구간(confidence interval, CI)을 제시하였다. p 값은 0.05 미만인 경우를 유의하다고 판단하였다. 연구결과 : 소 사육 작업 관련하여 농장이 HACCP(Hazard Analysis Critical Control Point) 인증을 받은 경우는 소 사육두수가 50두 미만인 경우에 비하여 50-99두, 100두 이상 사육하는 대상자에서 각각 2.0배(95% CI, 1.1-3.8), 5.4배(95% CI, 3.0-9.5) 유의하게 많았다. 최근 1년간 소 인공수정 작업에 참여한 경우는 사육 두수가 50두 미만인 경우에 비하여 50-99두, 100두 이상인 경우가 각각 1.8배(95% CI, 1.0-3.3)와 2.4배(95% CI, 1.4-4.2) 유의하게 많았다. 작업 중 소 분비물을 맨 손으로 접촉한 경우는 남성이 여성에 비하여 5.2배(95% CI, 1.7-15.7) 유의하게 많았으며, 소 사육 두수가 50두 미만인 경우보다50-99두, 100두 이상인 경우가 각각 2.6배(95% CI, 1.5-4.7)와 4.2배(95% CI, 2.4-7.4) 유의하게 많았다. 작업 이외 인수공통감염병과 관련된 요인 중에서 최근 1년 이내 소 생고기를 섭취한 경우가 남성이 여성에 비하여 2.4배(95% CI, 1.1-5.0) 유의하게 많았고, 소 사육 두수가 50두 미만인 경우에 비하여 100두 이상 사육하는 대상자에서 2.0배(95% CI, 1.0-3.8) 유의하게많았다. 최근 1년 이내 소 부산물을 생으로 섭취하는 경우는 남성이 여성에 비하여 4.0배(95% CI, 1.8-9.0) 유의하게 많았다. 소의 질병이 사람에게도 감염이 가능하다는 것을 인지하고 있는 경우는 교육 수준이 중학교 졸업에 비하여 고등학교 졸업에서2.5배(95% CI, 1.4-4.6) 유의하게 많았으며, 소 사육 두수가 50두 미만인 경우보다 50-99두, 100두 이상인 경우에서 각각3.3배(95% CI, 1.8-6.0)와 5.4배(95% CI, 2.8-10.4)로 유의하게 많았다. 결론 : 소 사육 두수가 적은 소규모 농장의 사육업자들에게는 인수공통감염병 인지율을 높이기 위한 교육과 HACCP 인증장려를, 소 사육 두수가 많은 사육업자들과 남성들은 보호구 착용 강화 및 식습관 개선에 중점을 두는 특성에 따른 맞춤형예방 대책 마련이 필요하다. Objective: We surveyed zoonoses related factors according to the personal characteristics of cattle farmers to establish customized policy to prevent zoonoses. Methods: We visited several group events of cattle farmers and conducted a questionnaire survey about the personal characteristics such as gender, age, educational background, working duration and the number of breeding cattle, and the zoonoses related factors linked to the breeding of cattle, eating habits and awareness among 407 cattle farmers. We selected the significant variables about personal characteristics which influenced the zoonoses related factors. Results: Among cattle farmers, the subjects who raise more cattle tended to obtain more HACCP (Hazard Analysis Critical Control Point) certification, participate in the work on artificial insemination of cattle more often, have more experiences of bare-hand contact with secretions of cattle during work, have more experiences of eating raw meat, and have high awareness rate about zoonoses. Male had more experiences of eating raw meat or residual product of cattle than female. Also, male had more experiences of bare-hand contact with secretions of cattle during work than female. Conclusion: For cattle farmers who raise cattle on a small scale, more education about zoonoses and encouragement of HACCP certification are necessary. For cattle farmers who raise cattle on a large scale and male farmers, it is necessary to pay attention to protective equipment wearing and improvement of eating habit to prevent zoonoses.