ORiginal Article : Gastroprotective Effects of PMK-S005 against Ethanol-Induced Acute Gastric Damage in Rats

( Yoon Jeong Choi ),( Nayoung Kim ),( Ju Yup Lee ),( Ryoung Hee Nam ),( Ji Hyung Seo ),( Seonmin Lee ),( Hee Jin Kim ),( Yoon Jin Choi ),( Hye Seung Lee ),( Dong Ho Lee ) 대한간학회 2016 Gut and Liver Vol.10 No.3

Background/Aims: This study aimed to examine the gastroprotective effects of PMK-S005, which is a synthetic S-allyl-Lcysteine (SAC; a sulfur-containing amino acid), against acute ethanol-induced gastric damage in rats. Methods: Sprague- Dawley rats were divided into six groups, including a nonethanol group, groups treated with absolute ethanol 1 hour after pretreatment with various doses of PMK-S005 (1, 5, and 10 mg/kg) or rebamipide (50 mg/kg), and an absolute ethanolonly group. Ethanol-induced gross ulcer and mucus levels were measured. Myeloperoxidase, tumor necrosis factor a, interleukin 1β, PGE2, LTB4, cPLA2, COX-1, and COX-2 levels were estimated by enzyme-linked immunosorbent assay or Western blot analysis. Furthermore, the protein expression levels of antioxidant enzymes, including heme oxygenase-1 (HO-1), NAD(P)H:quinine oxidoreductase 1 (NQO-1), GCLC, and GCLM, were assessed. Results: PMK-S005 significantly attenuated the ethanol-induced gastric damage; it reduced mucosal inflammatory cytokine production and increased mucus levels. The expression levels of cPLA2, COX-1, and COX-2 were decreased by PMK-S005. PMK-S005 did not affect PGE2 synthesis, but LTB4 production was significantly suppressed. In addition, long-term administration of PMKS005 significantly increased the expression of HO-1, NQO-1, GCLC, and GCLM. Conclusions: These results strongly suggest that PMK-S005 prevents gastric mucosal damage and that these gastroprotective activities are due to anti-inflammatory effects and enhancement of the gastric defense system, including antioxidant enzymes. (Gut Liver 2016;10:348- 355)

Vascular Protective Role of Samul-Tang in HUVECs: Involvement of Nrf2/HO-1 and NO

Choi, Eun Sik,Lee, Yun Jung,Seo, Chang Seob,Yoon, Jung Joo,Han, Byung Hyuk,Park, Min Cheol,Kang, Dae Gill,Lee, Ho Sub Hindawi Publishing Corporation 2016 Evidence-based Complementary and Alternative Medic Vol.2016 No.-

<P>Samul-Tang (Si-Wu-Tang, SMT), composed of four medicinal herbs, is a well-known herbal formula treating hematological disorder or gynecologic disease. However, vascular protective effects of SMT and its molecular mechanisms on the vascular endothelium, known as the central spot of vascular inflammatory process, are not reported. The aim of this study was to investigate vascular protective effects of SMT water extract in human umbilical vein endothelial cells (HUVECs). Water extract of SMT was prepared and identified by HPLC-PDA analysis. Expression of cell adhesion molecules (CAMs) and heme oxygenase-1 (HO-1) and translocation of nuclear factor-kappa B (NF-<I>κ</I>B) and nuclear factor-erythroid 2-related factor 2 (Nrf2) were determined by western blot. Nuclear localization of NF-<I>κ</I>B and Nrf2 was visualized by immunofluorescence and DNA binding activity of NF-<I>κ</I>B was measured. ROS production, HL-60 monocyte adhesion, and intracellular nitric oxide (NO) were also measured using a fluorescent indicator. SMT suppressed NF-<I>κ</I>B translocation and activation as well as expression of CAMs, monocyte adhesion, and ROS production induced by TNF-<I>α</I> in HUVECs. SMT treated HUVECs showed upregulation of HO-1 and NO which are responsible for vascular protective action. Our study suggests that SMT, a traditionally used herbal formula, protects the vascular endothelium from inflammation and might be used as a promising vascular protective drug.</P>

MICROWAVE GENERATION OF SPACE-CHARGE LIMITTED CURRENTS

서윤호 광운대학교 기초과학연구소 1997 기초과학연구소논문집 Vol.26 No.-

Dynamics of an intense relativistic electron beam above the space charge limitted current is investigated in order to identify the microwave generation characteristics of an oscillating virtual cathode in a TM resonant cavity. The coupling between the beam and the TM resonant modes is defined by modeling the electron beam with a one dimensional discrete sheets. The governing equations for the interaction are obtained. These equations form a basis of the numerical code that can predict the beam dynamics near the nonlinear saturation regime.

임신중 산전 초음파로 진단된 자궁근종의 임상적 고찰

서정호,김윤숙,김대원,이동운,최규연,이정재,이임순 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.1

Objectives: To study the clinical manifestations; time, mode of delivery, size of myomas and outcome of pregnancy in cases of myomas that were detected prior to pregnancy or during the antenatal care and after delivery. Methods: We statistically analyzed 76 patients with uterine myomas in pregnancy admitted to Soonchunhyang Seoul Hospital during the period of Jan. 1999 and Dec. 2001. Results: The results are as follows. 1. The mean age of patients in the detected group and undetected group were 32.9 years and 32.3 years, respectively. There was no difference in the proportion of primarity between the detected group(98.2%) and the undetected group(79.6%) 2. The most common type of uterine myoma in both groups was intramural myoma (detected group 94.1% vs undetected group 81.3%). The uterine myomas were most commonly located in the anterior portion and fundus(detected group 52.9% and 23.5% respectively vs undetected group 47.4% and 23.7% respectively) and the proportion was not significantly different between the two groups. Uterine myomas with a diameter of 3cm or more in the detected group were detected by clinical examination and ultrasonogram. Rate of uterine myomas with a diameter of 3cm or more in the detected group is 76.5% and less than 3cm is 54.2% which detected incidentally at delivery. 3. The proportion of term infants were detected group 88.2% vs undetected group 91.5%. 4. The most common indications for cesarean section in both groups were cephalopelvic disproportion and repeated cesarean section (detected group 41.2% and 45.8% respectively vs undetected group 11.8% and 18.6% respectively) and there was no significant difference between the two groups. 5. Secondary pathologic changes in the all myomectomy specimens were degeneration in both groups but there was no necrosis or sarcomatous change. 6. Antenatal complications were preterm labor, premature repture of membranes, spontaneous abortion, malpresentation, and placenta previa. Intrauterine growth retardation and placental abruption were not seen in both groups. Conclusion: Myoma detected by ultrasonogram before or during pregnancy is not a significant implication of pregnancy outcome.

개에서 발생한 자궁축농증 1례

서수원,손정민,윤기영,최호정,박성준,이영원,정성목,신상태,조종기 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2006 動物醫科學硏究誌 Vol.14 No.-

A 12-year-old, female miniature pinscher dog with history of fever and vaginal discharge 3 days ago was referred to the Veterinary Medical Teaching Hospital of Chungnam National University. A diagnosis of pyometra was made by survey of abdominal radiographs and ultrasonography. The uterus appeared as convoluted and tubular appearance filled with anechoic to hypoechoic fluid. Ovariohysterectomy was the choice of cervical patency. Medical therapy for pyometra may be appropriate in bitches that were of breeding age and that were not systemically ill. The surgery was fine. After 1 week, the dog was getting better.

초등학교 교육과정과 교과서에 농업·농촌의 기능 및 가치 반영 방안

서우석,강대구,정남용,김재호,이윤조 한국농업교육학회 2006 농업교육과 인적자원개발 Vol.38 No.4

이 연구는 농업ㆍ농촌의 기능과 가치를 재정립하고 이를 초등학교 교육과정 및 교과서에 반영시킬 수 있는 방안을 제시하는데 그 목적이 있었다. 이를 위하여 먼저 선행연구 고찰을 통하여 농업ㆍ농촌의 기능과 가치를 재개념화하고, 이를 토대로 내용 분석 틀을 개발하여 초등학교 교육과정 및 교과서를 분석 하고, 그 결과를 토대로 교육과정 및 교과서에 반영시키기 위한 방안을 제시하였다. 그 주요 결과는 다음과 같다. 첫째, 농업ㆍ농촌의 기능과 가치는 크게 자연환경적 기능(수자원 함양 기능, 환경정화 기능, 생태계 보전기능, 자연재해 방지 기능), 사회문화적 기능(전통문화 보전 기능, 정서의 순화 기능, 자연학습의장 제공 기능), 사회경제적 기능(농산물 공급 기능, 직업 기회 제공 기능, 국토의 균형발전 기능, 타 산업에의 기여 기능)으로 분류하였다. 둘째, 농업ㆍ농촌의 기능과 가치를 바탕으로 초등학교 교육과정 및 교과서를 분석한 결과, 전 학년에 걸쳐서 자연학습의 장 제공 기능과 전통문화 보전 기능이 높게 나타났고, 그 다음으로는 농산물 공급기능과 정서순화기능이 높게 나타났다. 반면에 수자원함양기능과 환경정화기능은 상대적으로 낮게 나타났다. 셋째, 내용분석을 토대로 교과의 성격을 고려하여 각 교과별로 농업ㆍ농촌의 기능과 가치를 반영시킬 수 있는 방안을 제시하였다. 마지막으로, 각 교과별로 농업ㆍ농촌의 기능과 가치 반영 방안을 체계적으로 이행하기 위한 행ㆍ재정적 지원 방안을 교육과정 개발 측면, 교과서 개발 측면, 교육과정 운영측면, 정책적 지원 측면으로 구분 하여 제시하였다. The purpose of the study is to suggest the strategies for incorporating the roles and values of agriculture & rural areas as a part of the national curriculum and elementary school textbooks. For this purpose, this study was conducted as follows; First, we reclassified the roles and values of agriculture & rural areas after critically reviewing literature: environmental conservation functions, socio-cultural functions and socio-economic functions. Second, based on the new classification, we develop a framework for analyzing the national curriculum and elementary school textbooks. The functions of rural areas as places for learning about nature and purifying emotion and the importance of agriculture in providing agricultural product were frequently presented but the functions of sustaining water resources and environmental purification were relatively less presented in the national curriculum and elementary school textbooks. Third, we suggested strategies for incorporating the roles and values of agriculture & rural areas in the national curriculum and elementary school textbook based on the analysis. Finally, we suggested necessary administrative and financial assistance to systematically and effectively implement the suggested strategies for developing a new curriculum and textbooks as well as implementing the curriculum.

사례기반 추론을 이용한 공정설계

서윤호,고범석,김용태 울산대학교 2002 공학연구논문집 Vol.33 No.1

This paper introduces a method to automate process design using Case Based Reasoning technique. Particularly, the function-behavior-structure model is used to represent a process plan as a case. Specifically, a case retrieval algorithm to use function and behavior, case adaptation knowledge definition by using neural network technique, and a machine selection method are presented.

영재교육기관의 교수·학습실태 분석 : 중학생 대상 과학영재교육

서혜애,이윤호 경북대학교 사범대학부속 중등교육연구소 2003 중등교육연구 Vol.51 No.2

The research aimed to investigate the current status of teaching and learning for the scientifically middle school students through survey questionnaire of 79 practicing science teachers at special classes in middle schools (SCMS) and gifted education institutions affiliated with university (GEIU). The most frequently used teaching methods appeared as hands-on activity at laboratory and discussion and presentation. For the level of teaching and learning for the gifted, it that teachers at GEIU perceived their teaching higher than teachers at SCMS. Teachers with master degrees perceived higher than those with or degrees. Participation in inservice training programs for gifted education positively influenced on their teaching level far teachers at SCMS. However, the apposite appeared for teachers at GEIU.

골아세포 MC₃T₃-E₁의 증식과 기능에 Insulin Like Growth Factor Ⅰ, Ⅱ 및 부갑상선 호르몬이 미치는 영향

윤현구,정호연,양인명,김성운,김진우,김광원,김영설,최영길,서광식 대한내과학회 1992 대한내과학회지 Vol.42 No.1

MC₃T₃-E₁골아세포군을 이용하여 IGF-Ⅰ과 Ⅱ를 투여하고 DNA합성과 골아세포기능을 측정하기 위해 PTH에 대한 C-AMP반응정도, alkaline phosphatase 활성도를 측정하여 다음과 같은 결과를 얻었다. 1) IGF-Ⅰ과 Ⅱ는 모두 DNA합성이 어느 농도까지는 증식효과를 나타내지만 그 이후에는 억제되는 양상을 보였다. 2) PTH에 대한 골아세포의 C-AMP반응으로 증가되었지만 IGF-Ⅰ과 Ⅱ의 투여시 농도변화에 따른 의미있는 변화는 없었다. 3) 본 실험의 조건에서 alkaline phosphatase 활성도는 증가되지 않았다. 이상의 결과로 MC₃T₃-E₁ 골아세포에 IGF-Ⅰ과 Ⅱ는 세포의 증식을 자극하여 PTH는 C-AMP생성을 촉진하는 효과가 있었다. The effect of insulin-like growth factor (IGF) Ⅰ and Ⅱ on cloned MC₃T₃-E₁ cells that had osteoblastic activity was examined by [³H] thymidine incorporation into DNA, cyclic AMP responsiveness to parathyroid hormone (PTH), and alkaline phosphatase activity. IGF Ⅰ and Ⅱ stimulated the growth and proliferation of osteoblastic cells at 20ng/㎖ and 50ng/㎖, respectively. We next examined the effect of IGF-Ⅰ and Ⅱ on the expression of osteoblastic activity. Adenylate cyclase response of PTH to IGF-Ⅰ and Ⅱ-treated cells showed maximal stimulation at 10 and 50㎚PTH, respectively, but IGF Ⅰ and Ⅱ induced limited activity of osteoblastic function. In conclusion, IGF-Ⅰ and Ⅱ were potent stimulators to the growth of osteoblastic cells but did not have an immediate effect on expression of osteoblastic function in this experiment.

새마을 主産團地의 營農實態 比較硏究

徐大振,李宗玉,鄭鉉承,崔元凱,姜鎬允 진주산업대학교 1981 論文集 Vol.19 No.-

The purpose of this study were to find the actual condition of the co-operative group farming in Korea, and to improve the low income Saemaul villages by the adoption of good points in co-operative Group farming. There were so many co-operative farmings in Korea, but all of them were not so successful, Eight co-operative group farmings were chosen to investigate for the above purposes. (Table 1). The tendency of agricultural income was not always true. First of all, Co-op Poultry farm did not follow the order of agricultural income. Because there was an excellent leader who had eliminated the circulation expenses in process of the purchase of feeds and the sale of eggs in common, Second, Seachiodong flower were devived into two parts the cultural flower and the sale flower. The cultural flower meant mainly producing flowers and the sale flower mainly selling flowers to downtown shops in Seoul. The income per householder of the cultual flower was less than that of the sale flower. Consequently, sucessful Co-operative Group farmings had an able leader, and performed production and sale more reasonablly.