한방 이화주의 미백 및 피부 주름 개선 효과

이상진(Sang-Jin Lee),권이영(Yi-Young Kwon),조성원(Sung-Won Cho),권희숙(Hee-Suk Kwon),신우창(Woo-Chang Shin) 한국식품영양과학회 2013 한국식품영양과학회지 Vol.42 No.4

본 연구에서는 한방이화주의 피부 생리기능 활성을 알아보기 위해 70% EtOH 추출물의 피부 미백, 주름 개선 및 항염증 효과를 조사하였다. HEE은 tyrosinase 활성 억제 및 tyrosine을 기질로 melanin이 형성되는 pathway에 관여하는 주요한 인자인 TRP-1과 TRP-2를 저해하는 작용 기전을 통해 피부 색소침착의 주요 원인 물질인 melanin 생합성을 농도 의존적으로 저해하는 것을 확인하였다. HEE은 피부진피 내 피부 탄력을 유지하는 elastin을 분해하는 효소인 elastase의 활성을 저해하였고, 피부의 keratinocyte가 생성분비하는 MMP-2와 MMP-9의 단백질 발현과 proteolytic 활성을 억제하여 노화에 따른 피부 주름 생성 억제할 수 있는 가능성을 확인하였다. 또한 세포 독성 없이 LPS에 의해 유도된 염증 반응을 50% 저해하는 HEE의 농도(IC50)는 24.9 μg/mL이며, 50 μg/mL 농도로 처리하였을 때 염증 반응 저해 효과가 70%로 높은 효과를 가지는 것을 확인하였다. 이상의 결과를 종합하면 HEE의 피부 미백, 주름 개선 및 항염증에 우수한 효과를 나타내고 있으므로 기능성 화장품의 주요한 소재로 이용 가치가 높을 것으로 사료된다. Extrinsic skin aging is characterized by the loss of skin tone and resilience, irregular pigmentation, and deep wrinkles. The aim of this study was to investigate the effects of Ehwa Makgeolli containing oriental herbs (Glycyrrhiza uralensis Fisch., Lycium chinense MILL., Morus alba L., and Saururus chinensis Baill) on skin whitening and wrinkling in human skin cells. We prepared Makgeolli extracts (HEE) with 70% ethanol. HEE significantly inhibited in vitro mushroom tyrosinase activity and reduced the cellular and secreted melanin content of mouse melanoma melanocytes (B16F1 cells). HEE down-regulated the protein expression of tyrosinase related protein (TRP)-1/-2, a key player in melanogenesis. Treatment with HEE in human keratinoctyes (HaCaT cells) inhibited the proteolytic activities of matrix metalloproteinase (MMP)-2/-9 in a dose-dependent manner and dramatically reduced the expression of MMP-2/-9. In addition, HEE attenuated lipopolysaccharide (LPS)- induced nitric oxide production in murine macrophages (RAW264.7 cells). These results indicate that HEE may be a great cosmeceutical ingredient for its whitening, anti-wrinkle, and anti-inflammatory effects.

T Cell-Specific Knockout of STAT3 Ameliorates Dextran Sulfate Sodium-Induced Colitis by Reducing the Inflammatory Response

Kwon, Sun-Ho,Seo, Eun-Bi,Lee, Song-Hee,Cho, Chung-Hyun,Kim, Sung Joon,Kim, Sang Jeong,Kim, Hang-Rae,Ye, Sang-Kyu 한국조명·전기설비학회 2018 한국조명·전기설비학회 학술대회논문집 Vol. No.

<P>Signal transducer and activator of transcription 3 (STAT3) has a crucial role in various autoimmune disorders including, inflammatory bowel disease (IBD). Our previous study demonstrated that STAT3 activation by IL-6 in colonic epithelial cells exacerbates experimental ulcerative colitis. Activated T lymphocytes are also found in ulcerative colitis patients with intestinal inflammation, but the role of <I>STAT3</I> in T cells remains elusive. To determine the <I>STAT3</I> function of T cells in intestinal inflammation, we generated T cell-specific STAT3 knockout (KO) mice and used dextran sulfate sodium (DSS) to induce colitis. In this study, we demonstrated that T cell-specific <I>STAT3</I> deletion alleviated DSS-induced colitis in mice, resulting in reduced histological scores and myeloperoxidase (MPO) activity. Importantly, the population of T cells in the spleen and lymph nodes was significantly decreased in the control and DSS-induced groups of STAT3 KO mice. In addition, <I>STAT3</I> deficiency in T cells markedly reduced the production of interferon (IFN)-γ, IL-6, and IL-17A, whereas IL-10 secretion was increased. Collectively, the results suggest that <I>STAT3</I> in T cells may be a therapeutic target in ulcerative colitis by balancing the immune response through T cell homeostasis.</P>

Development of New Preventive and Therapeutic Vaccines for Tuberculosis

Kwon, Bo-Eun,Ahn, Jae-Hee,Min, Seunghwan,Kim, Hyeongseop,Seo, Jungheun,Yeo, Sang-Gu,Ko, Hyun-Jeong 한국조명·전기설비학회 2018 한국조명·전기설비학회 학술대회논문집 Vol. No.

<P>Tuberculosis (TB) is a contagious disease that has been responsible for the death of one billion people in the last 200 years. Until now, the only vaccine approved for the prevention of TB is Bacillus Calmette-Guérin (BCG), which is prepared by attenuating <I>Mycobacterium bovis</I>. However, one of the limitations of BCG is that its preventive effect against pulmonary TB varies from person to person. Therefore, there arises a need for a new TB vaccine to replace or supplement BCG. In this review, we have summarized the findings of current clinical trials on preventive and therapeutic TB vaccine candidates. In addition, we have discussed a novel vaccination approach using the cell-based vaccine presenting early secretory antigenic target-6 (ESAT-6), which is a potent immunogenic antigen. The role of ESAT-6 in hosts has also been described.</P>

Impact of Prior TACE History on the Outcome of HCC Patients Undergoing Molecular Targeted versus Immune- Based Therapy: A Real-World Analysis

( Kwon Yong Tak ),( Ji Hoon Kim ),( Ahlim Lee ),( Ji Won Han ),( Soon Kyu Lee ),( Hyun Yang ),( Hee Chul Nam ),( Pil Soo Sung ),( Hee Yeon Kim ),( Seok Hwan Kim ),( Hae Lim Lee ),( Myeong Jun Song ),( 대한간학회 2023 춘·추계 학술대회 (The Liver Week) Vol.2023 No.1

Microbiological survey of Korean animal facilities from 2014 to 2019

Sang woon Kim,Hi jo SHIN,Se hee PARK,Teak chang LEE,Hae jin LEE,Ok sung MOON,Ki hoan NAM,Won kee YOON,Hyoung chin KIM,Hyo jung KWON,Young Suk WON 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

To survey the mouse microbiological contamination rate in Korean animal facilities, we tested for common contaminants in these facilities using serological, culture, and parasitological methods. A total of 21,292 experimentally housed mice from 206 animal facilities, including hospitals, universities, companies and research institutes, were tested over a 6-year period from 2014 to 2019. The most commonly found contaminants were various species of non-pathogenic protozoa (47.2%). The most common pathogenic bacteria were Staphylococcus aureus (21.2%), Pasteurella pneumotropica (12.5%) and Pseudomonas aeruginosa (5.8%). Mouse hepatitis virus (6.1%) was detected, but no other viral or bacterial pathogens were found. These results establish that the main pathogens that currently contaminate animal facilities in Korea are opportunistic pathogens, whereas important such as those in categories B or C, were decreased.

The Effect of Living Conditions on Stress and Behavior of Horses

( Sang Kook Park ),( Hee Jun Jung ),( You Lim Choi ),( Oh Sub Kwon ),( Young Hun Jung ),( Chung Il Cho ),( Min Jung Yoon ) 한국동물자원과학회(구 한국축산학회) 2013 한국축산학회지 Vol.55 No.4

Providing an adequate environment for horses is important to minimize the level of stress for domesticated horses. The objectives of this study were 1) to evaluate the effect of living conditions on stress level of horses, 2) to observe the effect of one month confinement on self-maintenance behavior and stereotypic behavior of horses. The experiment was conducted at National Institute of Animal Science, Equine Field Station(Seonghwan-eup, Korea). Horses were staying in the paddock prior to the experiment. On day 1, five horses were randomly selected and housed in metal fence panels stall. Six horses remained in the same paddock. The ratio of neutrophil to lymphocyte (on day 15) and cortisol (on day 1 and 29) from stalled horses were significantly higher than horses in the paddock. Duration or frequency of self-maintenance behaviors such as feeding, drinking, resting, walking was not significantly different between day 1 and day 29. However, the frequency of urination significantly decreased(p<0.05) on day 29 compared with day 1. The frequency of stereotypic behaviors was not different between day 1 and 29. Our data indicate that horses may be more stabled when they are staying in the paddock rather than staying in the stall, but the stress level of horses in the stall during one month confinement was not effective for horses to adapt stereotypic behavior. In conclusion, providing an adequate environment and stress-less horse management techniques can minimize the stress level of horses.

Grasp Behavior Analysis Using Muscle and Postural Hand Synergies for Smartphones

Sung Hee Ahn,Sanghyun Kwon,Youngjin Na,Myunghwan Yun 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.22 No.4

Smartphones are currently among the most common handheld devices. Previous studies on such handheld touchscreen devices focused on thumb operations or reach zones by measuring individual muscle or joint angles. However, they were limited to thumb operations and did not consider grasping. In this study, we investigated the grasp types of touchscreen devices and other objects included in an existing grasp taxonomy. To this end, principal component analysis and latent profile analysis clustering were used for extracting and grouping muscle and postural synergies. Fourteen healthy subjects performed up to 15 hand grasps, including that with a smartphone. Electromyography (EMG) data were measured on six muscles in the forearm and the hand, and joint angles were measured for 22 joints in the hand. The first two muscle synergies from the EMG data and the first three postural synergies from the kinematic data were found to account for over 60% of the overall grasping. In terms of the synergies, the grasp for handheld touchscreen devices showed unique characteristics in terms of muscle and postural synergies compared to other objects. The obtained results may aid in understanding of grasping behaviors for handheld touchscreen devices in various applications.

Interim Results of an Automated Electronic Medical Record-Based Alert System for the Micro-Elimination of Hepatitis C - A Single Center-Based Experience

( Hee Yeon Kim ),( Sung Won Lee ),( Jung Hyun Kwon ),( Chang Wook Kim ),( Se Hyun Cho ),( Hee Chul Nam ),( Hyun Yang ),( Do Seon Song ),( U Im Chang ),( Jin Mo Yang ),( Hae Lim Lee ),( Soon Woo Nam ) 대한간학회 2023 춘·추계 학술대회 (The Liver Week) Vol.2023 No.1

Blockade of cytokine-induced endothelial cell adhesion molecule expression by licorice isoliquiritigenin through NF-kappaB signal disruption.

Kwon, Hyang-Mi,Choi, Yean-Jung,Choi, Jung-Suk,Kang, Sang-Wook,Bae, Ji-Young,Kang, Il-Jun,Jun, Jong-Gab,Lee, Sang-Soo,Lim, Soon Sung,Kang, Young-Hee The Society 2007 Experimental biology and medicine Vol.232 No.2

<P>Numerous polyphenolic compounds have been found to inhibit adhesion and migration of leukocytes to sites of inflammation that are partly regulated by the expression of cell adhesion molecules (CAM) such as vascular cell adhesion molecule-1 (VCAM-1), E-selectin, and platelet endothelial cell adhesion molecule-1 (PECAM-1). Licorice root extracts have been used in traditional Chinese, Tibetan, and Indian medicine for the treatment of pulmonary diseases and inflammatory processes. Expression of CAM proteins was examined in human umbilical vein endothelial cells (HUVEC) treated with a licorice component (isoliquiritigenin, 18beta-glycyrrhetinic acid, glycyrrhizin, formononetin, or ononin) and exposed to TNF-alpha. The involvement of NF-kappaB in the transcriptional control of CAM proteins was assessed by degradation of IkappaBalpha and nuclear translocation of NF-kappaB using Western blotting techniques and immunocytochemical staining. At nontoxic > or =10 microM, isoliquiritigenin blocked the induction of VCAM-1 and E-selectin on activated HUVEC and markedly interfered with THP-1 monocyte adhesion to TNF-alpha-activated endothelial cells. Isoliquiritigenin abolished TNF-alpha-induced mRNA accumulation of VCAM-1 and E-selectin. Additionally, immunocytochemical staining revealed that isoliquiritigenin attenuated PECAM-1 expression induced by TNF-alpha. In contrast, other components recognized in licorice, 18beta-glycyrrhetinic acid, glycyrrhizin, formononetin, and ononin did not down-regulate the expression of VCAM-1 and/or PECAM-1 activated by TNF-alpha, implying that these components are inactive in modulating adhesion of leukocytes to stimulated endothelial cells. Isoliquiritigenin downregulated CAM proteins in TNF-alpha-activated HUVEC at the transcriptional levels by blocking degradation of IkappaBalpha and nuclear translocation of NF-kappaB. These results demonstrate that the induction blockade of VCAM-1 and E-selectin by isoliquiritigenin was directly mediated by its interference with the CAM mRNA transcription through NF-kappaB-dependent mechanisms under inflammatory conditions.</P>

Fms-like tyrosine kinase 3 is a key factor of male fertility

Kwon, Woo-Sung,Kim, Ye-Ji,Ryu, Do-Yeal,Kwon, Ki-Jin,Song, Won-Hee,Rahman, Md Saidur,Pang, Myung-Geol Elsevier 2019 Theriogenology Vol.126 No.-

<P><B>Abstract</B></P> <P>Fms-like tyrosine kinase 3 (FLT3) is a type III kinase that is highly expressed in seminal plasma of infertile men. FLT3 activation can be blocked by inhibition of its phosphorylation using the nontoxic and selective inhibitor, quizartinib. We investigated the function of FLT3 and the corresponding effects of quizartinib in mouse spermatozoa. Spermatozoa were treated with different concentrations (0.1, 1, 10, 20, and 30 μM) of quizartinib for 90 min at 37 °C in 5% CO<SUB>2</SUB> in air. FLT3 was detected in capacitated and non-capacitated spermatozoa. While the level of FLT3 was unaffected, the levels of phospho-FLT3 were significantly altered in spermatozoa by quizartinib. Exposure of spermatozoa to higher concentrations of quizartinib significantly altered sperm viability, motility, motion kinematics, levels of intracellular ATP, and capacitation status. Fertilization and early embryonic development were suppressed by quizartinib. This may have occurred as a consequence of decreased protein kinase A (PKA) activity and tyrosine phosphorylation. The inhibition of FLT3 by quizartinib may affect the fertilization and embryonic development by reducing tyrosine phosphorylation through a PKA-dependent pathway. Our data implicate FLT3 as a biomarker for diagnosis and prognosis of male fertility. In addition, quizartinib has potential for development as a new contraceptive agent.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Inhibition of FLT3 in spermaotozoa using quizartinib significantly altered various sperm functions. </LI> <LI> The inhibition of FLT3 significantly decreased protein kinase A (PKA) activity and tyrosine phosphorylation. </LI> <LI> In addition, fertilization and early embryonic development were suppressed by inhibition of FLT3. </LI> <LI> We suggest that FLT3 can be used as a biomarker for the diagnosis and prognosis of male fertility. </LI> </UL> </P>