Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions

Lee, Sang Choel,Han, Seung Hyeok,Li, Jin Ji,Lee, Sun Ha,Jung, Dong-Sub,Kwak, Seung-Jae,Kim, Seung Hye,Kim, Dong Ki,Yoo, Tae-Hyun,Kim, Jin Hyun,Chang, Se-Ho,Han, Dae Suk,Kang, Shin-Wook International Society of Nephrology 2009 Kidney international Vol.76 No.8

Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose–treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose–treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions.

Gliotoxin ameliorates TNBS-induced colitis through regulation of NF-kB, and up-regulation of HO-1

김상욱 ( Kim Sang Ug ),( Jay Min Oh ),( Yeon Hwa Kim ),( Jung Moo Hur ),( Kyo Sang Yoo ),( Yong Sung Kim ),( Joo Jin Yeom ),( Tae Hyeon Kim ),( Suck Chei Choi ),( Chang Duk Jun ),( Yong Ho Nah ) 대한소화기학회 2003 대한소화기학회 추계학술대회 Vol.2003 No.-

<Background & aims> During inflammation in colon, cells of the gut mucosa produce or express various inflammatory mediators, including IL-8, TNF-a and IL-1b, and intercellular adhesion molecule 1 (ICAM-1). These cytokines and chemokines have recently been

대호 간척기 토양의 염농도별 밭작물의 염해 평가

이승헌,류순호,설수일,안열,정영상,이상모 江原大學校 附設 環境硏究所 2001 環境硏究 Vol.18 No.-

This study was carried out to obtain the basic data for selecting the applicable crops in reclaimed land during desalinization period. A pot experiment was conducted with 5 different electrical conductivities of the saturated extracts (ECe 1, 3, 9, 14, and 16 dS·m^(-1)) of soils taken from the Dae-Ho reclaimed tidal lands. Eight crops (Chinese cabbage, radish, tomato, red pepper, buckwheat, soybean, sesame, and green perilla) were grown for 37days. Plant height and number of leaves were surveyed on 2 and 4 weeks after seeding, and on harvest time (5 weeks). After harvest, dry weights of harvested crops were measured and soil chemical properties were analyzed. Emergence rates of crops were comparatively high except sesame. For sesame, there was no emergence at ECe over 3 dS·m^(-1). Growth and dry weight decreased significantly as increasing ECe. The ECe which decreased 50% of dry weight index were 14.2 dS·m^(-1) for radish, 11.4 dS·m^(-1) for Chinese cabbage, 10.2 dS·m^(-1) for tomato for red pepper, 8.9 dS·m^(-1) for buckwheat and green perilla, 8.6 dS·m^(-1) for soybean, and 8.9 dS·m^(-1) for tomato. At higher ECe that start the growth inhibition, increasing 1 dS·m^(-1) in ECe, 7.7, 6.5, 5.9, 5.6, 5.2, and 4.9% of dry weight decreased for buckwheat, green perilla, Chinese cabbage, radish, soybean, and tomato (red pepper), respectively. The critical value of ECe for crop survival except sesame was 15.4~23.1 dS·m^(-1).

간세포암의 십이지장침윤에 의한 상부위장관 출혈 1 예

유태현,한광협,박성하,김형길,문영명,한재호,조상호 대한소화기내시경학회 1999 Clinical Endoscopy Vol.19 No.2

Recent advances in both the diagnosis and treatment of hepatocellular carcinoma have improved the prognosis and changed the clinical significance of the recently increasing distant metastases. Distant metastases found after successful treament of the primary lesions are of great clinical significance for the treatment of hepatocellular carcinoma. The duodenum is a rare site of hematogenous metastases or direct invasion from hepatocellular carcinoma. A 23 year old man was admitted with upper gastrointestinal bleeding. He had been diagnosed with hepatocellular carcinoma and treated by a left lobectomy and chemoembolization. The patient was admittted for 12 months after the treatment of the primary tumor. Endoscopic examination revealed a mass in the duodenal bulb that protruded into the lumen. He died due to massive hematemesis. We report on a rare case of hepatocellular carcinoma with duodenal invasion in a 23-year-old male patient.

Z-plasty를 이용한 설유착증의 치료증례

유정호,이상호,유종만 大韓小兒齒科學會 1991 大韓小兒齒科學會誌 Vol.18 No.2

Ankyloglossaia, or "tongue-tie", is a congenital condition which occurs as a result of fusion between the tongue and the floor of the mouth. The condition can vary in degree from a partial to a complete ankyloglossia. Limitation of movement of the tongue tip results in malocdlusion whth an anterior "open bite" deformity, early prognathism, and swallowing, speech and respiration difficulties. A method of Z-plasty on tongue-tie have been concermed since 1966s. A report of children who have been treated with frenectomy, myotomy & Z-plasty due to ankyloglossia were presented. Generally, lingual frenectomy was used for Tx. of ankyloglossia but extension and relapse were found out fault. In this treatment, we used Z-plasty. as a result we finded out that it is effective for correction of movement limitation of tongue, prevention of relapse, lengthing of mucosal tissue. Further, periodic check up is needed for evalation of relapse, improvement of speech, and other function of tongue.

협착관내에서 멱법칙 비뉴턴 유체와 점탄성 유체의 유동특성에 관한 연구

유상신,서상호 한국항공대학교 1993 論文集 Vol.31 No.-

비뉴턴 유체의 구성방정식 중에서 수정멱법칙모델을 이용하여 원형단단면을 갖는 직선관, 급격축소-급격확대 협착관, 그리고 점차축소-점차확대관 내에서의 유동특성을 수치모사하였다. 점차축소-점차확대관은 직선으로 연결되는 경우와 코사인곡선으로 이루어지는 경우로 구분하였다. 비뉴턴 유체중에서 저항감소현상을 나타내는 점탄성유체를 이용하여 급격축소-급격확대 협착관에서의 유동특성을 실험적으로 결정하였다. 완전히 발달된 층류유동에서 멱법칙 지수 n가 작아질수록 속도 형상은 관의 중앙부분에서 평탄해지고 압력손실은 증가한다. 급격축소-급격확대 협착관이 점차축소-점차확대협착관에서 곡선의 영향은 별로 크지 않다. 점탄성 유체의 압력손실계수는 층류영역에서 Reynolds수의 함수로 나타나며, 물보다 크게 나타난다. 협착비율과 협착길이비가 클수록 압력손실계수는 크게 나타난다. The modified power law model for a non-Newtonian fluid is employed to simulate the interanal flows of smooth straight tube, sudden contraction-sudden sxpansion tube, and gradual contraction-gradual expansion tube. Geometries of the gradual contraction-gradual expansion tubes are made of straight line or cosine curve. Experimental investigations using the drag reducing polymer solutions that are known as the viscoelastic fluids are conducted for the internal flows with sudden cotraction-sudden expansion tubes. The velocity profile in the contral region becomes flat and the pressure loss increases as the power law index n decreases in the fully developed laminar flow. The pressure loss of the sudden contraction-sudden expansion tube is larger than that of the gradual contraction-gradual expansion tube. The effect of index n on the sudden contraction-sudden expansion is greater than that on the gradual contraction-gradual expansion tube. The curvature effect of the gradual contraction-gradual expansion tube is not pronounced. The pressure loss coefficients of viscoelastic fluid is larger than that of water for the same Reynolds number. The loss coefficient of the stenosed tube increases as the percent contraction ratio and the length to diameter ratio increase.

정기적인 수영훈련이 어린이들의 신체작업능력 및 심폐기능에 미치는 영향

유승희,심성섭,박찬호 경희대학교 체육과학연구소 1995 體育學論文集 Vol.23 No.-

The following results have been won after the periodical experiment by way of multistage loading by using the cycle ergometer for the concrete items about Physical Fitness, Physical work Capacity and cardiovascular function to investigate the effects of the swimming to the boys and girls each numbered 20(in the 4th, 5th, and 6th grade in the elementary School) who have been the objects of the experiments and have been participating in the swimming training periodically and other boys and girls each numbered 20 who have not been participating and have not experienced such physical exercises. 1.In the comparison about the physicla Fitness, In case of the boys participating group showed higher than the ordinary group by Back strength 1.69kg, grip strength 2.43kg, Arm Flexion strength 0.75kg, Leg Extension strength 2.4kg and Trunk Flexion 2.11cm while the ordinary group showed higher in the jump step test by 1.7beats than the participating group but not so significant difference. 2.In the comparison about the physical Fitness, in case of the girls participating group showed higher than the ordinary group by Back strength 0.52kg, grip strenght 2.14kg, Arm Flexition strength 2.5kg, each Leg Extension strength 8.88kg, Trunk Flexion 1.34cm and 1.3beats in the Jump step test and the Leg Extension strength has been higher( p<.05) by notable difference. 3.In the Physical work capacity, the participating group showed higher than the ordinary group by 0.38 minutes in the boys and 2.01minutes in the girls group the not able difference appeared(p<.05) and the maximum exercise hour showed higher in the participating group by 1.03 minutes in the boys(p <.05) and in the girls by 2.24 minutes(p<.01). 4.In the comparison about the cardiovascular function participating group showed lower by respiration of single ventilation and Heart rate in one time also in the respiration rate, Q2 pulse, Oxygen uptake, ventilation of carbon dioxide, oxygen uptake per weight, rate of gas exchange the participating group showed lower but not notable difference in the boys group. In the maximum value the participating group showed higher by the notable differences(p<.01) in ventilation of carbon dioxide and oxygen uptake per weight but in one time ventilation it showed lower. And in the oxygen uptake per weight, oxygen uptake and rate of gas exchange there appeared notable differences(p<.05) In the participating group by higher and there have been no notable differences in the respiration rate and Heart rate. 5.In the comparison about the cardiovascular function, in case of girls, when they are in the stable state, the Heart rate and rate of gas exchange showed little lower in the participating group but in the respiration of one time ventilation, respiration rate, Q2 pulse, oxygen uptake, ventilation of carbon dioxide and oxygen uptake per weight they showed higher but no notable differences. In the maximum value there showed higher by the notable differences(p<.05) in the respiration of one time ventilation of carbon dioxide, O2 pulse of the participating group and in the respiration rate and rate of gas exchange it showed little higher and in the Heart rate it showed little lower but no notable difference.

십이지장 궤양 환자와 만성 위염 환자의 염증 분포의 차이 및 H. pylori 집락도와 염증도의 상관관계

유광하,진춘조,박형석,이재동,김예리,윤성욱,이수인,윤호상 건국대학교 의과학연구소 1998 건국의과학학술지 Vol.8 No.-

An inflammatory difference between the gastric antrum, body and fundus was studied in 39 H.pylroi positive duodenal ulcer and 29 H. pylori chronic gastritis patients. Also we studied correlation of H. pylori colony count and inflammatory severity. Patients with newly diagnosed duodenal ulcer and chronic gastritis were endoscoped and two biopsy secimens each taken from the antrum, body and fundus. Although intestinal metaplasia in antrum and inflammatory activity in fundus were more severe in chronic gastritis patients. another inflammatory difference was equally likely to be found between two groups. There was no definite histological inflammatory difference between duodenal ulcer and chronic gastritis patients. A positive correlation was found between the number of H. pylori and the severity of inflammation.

경동맥분지관 모형내 유동현상의 연구

유상신,서상호,정태섭 한국항공대학교 1996 論文集 Vol.34 No.-

경동맥분지부내 혈액유동을 촬영하는 자기공명혈관촬영술은 경동맥외벽을 따라 발생되는 신호소실로 인하여 때로는 실제 혈류현상을 잘 나타내지 못한다. 본 연구에서는 경동맥분지부에서 신호소실의 원인이 되는 유동의 역류 현상을 실험 및 수치해석적 방법으로 연구하고자 한다. 실험은 생체용 수지로 만든 경공맥분지관의 보형을 이용하고 유동형태는 4w% 설탕수용액으로 채워진 재순환장치에 설치된 모형에서 자기공명혈관촬영술과 색혈류영상장치를 이용하여 관찰하였다. 수치해석을 위한 경동맥의 이상화된 기하학적 형상은 제작된 모형과 동일하게 하였다. 정상상태의 운동량방정식을 유한체적법으로 수치해를 구하였다. 자기공명촬영술과 색혈류영상장치로 관찰한 모형내 유동현상은 수치 해석결과와 잘 일치한다. Magnetic resonance angiograms for blood flows in the carotid artery bifurcation sometimes fail to exhibit the real flow phenomena due to the signal dropout phenomenon along the posterior wall of carotid sinus where the reversal flow prevails. The objective of this study is to investigate the flow reversal phenomenon in the carotid artery. A phantom of the carotid bifurcation made of bioacrylic resin is used to observe the flow phenomena in the carotid artery. Flow patterns are observed by using magnetic resonance angiogram and color Doppler imaging systems for flows in the phantom of an automatic closed-type circulatory system filled with sugar 4 weight percent solution. For numerical analysis the idealized geometric shape of the carotid artery is constructed to portray the phantom. Steady momentum equation is solved by the finite volume method. Results of numerical analysis are compared with those of magnetic resonance angiograms and color Doppler images. The flow phenomena observed in the phantom on magnetic resonance angiogram and color Doppler imaging are identical to those of the numerical results.

국균세포벽을 모델로 한 새로운 고정화담체의 개발

유은정,김종덕,송상호,임현수,최명락,정규화 여수대학교 1997 論文集 Vol.11 No.2

The purpose of this investigation was to develop a new carrier using chitin and glucan as the model for Aspergillus oryzae cell wall. In case of the ratio of chitin and glucan(3/1), the activity of the immobilized protease had higher than that of model(chitin/glucan=1/3) and carriers were formed gel. In case of soluble chitin and glucan treated with calcium carbonate, there were a 1.8-fold increase in activity of immobilized protease compared with untreated chitin. The scanning electron microscope image showed that a pore size of this carrier had a homogenized pore size and a smooth surface.