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소의 시상하부내에서 Vasopressin과 Oxytion분비세포의 분포에 관한 면역조직화학적 연구
유명철,김종중,장인엽,문정석,정주현,김흥중,조사선 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.1
This study was done to observe the distributions of Oxytocinergic and Vasopressinergic neurons in the hypothalamus of the cow. For the immunohistochemical staining, sections were reacted with the following sequence of solutions: monoclonal anti-vasopressin and anti-oxytocin, biotinylated anti-mouse IgG, avidin-biotin peroxidase complex, and Ni-DAB. The results observed under light microscope were summarized as follows. Our immunohistochemical investigations reveal both vasopressinergic and oxytocinergic neurons in the supraoptic nucleus(SON), paraventricular nucleus(PVN), accessory supraoptic nucleus (ASN), periventricular nucleus(PN), suprachiasmatic nucleus(SCN), and arcuate nucleus(AN), but these two types of neurons were mainly distributed in the PVN and SON and the ratio of the number of vasopressinergic neurons to that of oxytocinergic neurons was 1:1.30 in the PVN, and 1:1.00 in the SON. These two types of neurons were round, oval and spindle-shaped and cell size was 30-55um.
골프장의 잔디 토양에서 살균제 Tolclofosmethyl의 분해
정근욱,우선희,김흥태,사동민,김영기,홍순달,김재정,이재구 한국환경농학회 2004 한국환경농학회지 Vol.23 No.3
Tolclofosmethyl (TCFM) is heavily and annually applied to the turf soils of most golf courses in Gyeongju to control the fungi known to cause the disease brown patch. The soil samples used for the experiment was collected three weeks after the annual application at the end of May in the year 2002. The preliminary results obtained from this study demonstrated that the repeated field annual applications of TCFM to the turf soils of a golf course located in Gyeongju city in the southern area of Korea showed the enhanced degradation of the parent compound TCFM, especially in the surface (0~15 cm) soil rather than the shallow subsurface (15~30 cm) and deep subsurface (30~45 cm) soils, compared to the corresponding surface (0~15 cm) and shallow and deep subsurface (15~30 cm and 30~45 cm) soils of the untreated plot. It appears that microorganisms in the soil may be involved in the enhanced degradation of TCFM.
Kim, Jin-Woo,Seo, Chun-Ok,Cho, Eun-Young,Kin, Heung- Kee,Kim, Sa-Jin,Hur, Soo-Young,Kim, Young-Wook,Park, Tae-Chul,Lee, Joon-Mo,Namkoong, Sung-Eun 가톨릭대학교 2000 Bulletin of The Catholic Research Institutes of Me Vol.28 No.-
Purpose : Cancer of the uterine cervix remains the leading cause of cancer death in Korean women. Conventional examinations still have limitations with regards to sensitivity and specificity in diagnosis and to monitoring for the disease. Thus, an additional specific tumor marker is needed of early detection of recurrent uterine cervical carcinoma and for estimation of patient's prognosis. Materials and Methods : Monoclonal antibodies against human cervical carcinoma were generated using hybridoma technology. These murine monoclonal antibodies were produced by fusion of spleen cells obtained from mice immunized with GUMC-6, a human cell line-of squamous cell carcinoma derived from uterine cervix, and P3-X63-Ag8 mouse myeloma cells. Results : We obtained 415 hybridomas secreting specific monoclonal antibodies to cervical carcinoma antigen continuously. Among them, one hybridoma designated CC5 that was highly reactive with cervical carcinoma was selected, and examined on the staining pattern and the reactivity with antigenic determinants of cervical carcinoma. Immunohistochemica staining revealed that CC5 monoclonal antibody reacted with all of the seven cervical carcinoma tissues, but also reacted with one of the ten (10%) normal cervical tissues. Western blot analysis showed that CC5 monoclonal antibody detected single 19.5-kDa sized protein b and in cervical cancer patient's sera. The detection rate was 88% (7/8). However, CC5 monoclonal antibody did not show any reactivity to 15 sera of normal healthy women tested. Sodium dodecyl sulfate polyacrylamide gel electrophortic(SDS-PAGE) analysis of CC5 monoclonal antibody immunoprecipitates of extracts of L-□ methionine-labeled human cervical carcinoma cells showed a major band in apparent molecular weight, 51,000 daltons. The isotype and subclass of CC5 monoclonal antibody was lgG2b in hemagglutination assay. Conclusions : We have developed a new monoclonal antibody, CC5, against squamous cell carcinoma of the human uterine cervix. Further investigation is needed to determine this monoclonal antibody as an immunodiagnostic devise for cervical cancer. (Journal of Korean Cancer Association 31(3):562-574, 1999)
Kim, Sa-Rin,Kim, Jae-Geun,Ju, Eun-Jeong,Lee, Yang-Woo,Lee, Bo-Ah,Kim, Heung-Tae,Nam, Jong-Min The Ecological Society of Korea 2006 Journal of Ecology and Environment Vol.29 No.5
The composition changes of litters were investigated to figure out the effects of the decomposition of Humulus japonicus on nutrient circulation and decomposition process in the riverine wetlands: Tan stream and Amsa-dong. Litterbags (mesh size 1 mm and 5 mm) were installed to monitor mass and nutrient changes of 5 types of litters: H. japanicus only, Miscanthus sacchariflarus only, Phragmites communis only, mixed litters including H. japonicus, and mixed litters without H. japonicus for 7 months. It was shown that k (decay rate) of the H. japanicus ($2.68{\sim}3.12$) was higher than that of M. sacchariflorus ($1.83{\sim}2.16$) and P. communis ($0.02{\sim}1.18$). The mass and organic remainings of the mixed litters including H. japonicus at Tan stream were $47.0{\sim}55.1%\;and\;47.0{\sim}54.9%$ and those of the litterbags without H. japanicus were $49.2{\sim}65.4%\;and\;47.1{\sim}57.5%$, respectively. This result indicated that the nutrient circulation was faster at H. japanicus community than others. Ca, Na, Mg, K, P, C, N and H contents reduced to around $40{\sim}80%$ of original. However, Na concentration increased up to $407{\sim}584%$ at 100 days and decreased to $248{\sim}498%$ at the end of the experiment. Decomposition rates were similar between 1 mm and 5mm mesh size litterbags and this implies that plant litters in studied areas decomposed mainly by microbes rather than small animals. This study revealed that the fast growth of H. japonicus was resulted from fast decomposition in part: positive feedback of nutrient cycling.
Development of On-Line Health Monitoring System for Pipes in Nuclear Power Plants
Eom, Heung Seop,Lim, Sa Hoe,Kim, Jae Hee,Kim, Young H.,Kim, Hak Joon,Song, Sung Jin Trans Tech Publications, Ltd. 2006 Key Engineering Materials Vol.321 No.-
<P>This study was aimed at developing an effective method and a system for on-line health monitoring of pipes in nuclear power plants by using ultrasonic guided waves. For this purpose we developed a multi-channel ultrasonic guided wave system for a long-range inspection of pipes and a few techniques which can effectively find defects in pipes. To validate the developed system we performed a series of experiments and analyzed the results.</P>