Fisheries resources management of crucian carp based on assessment of fish stock and potential yield in the mid-upper system of Seomjin River

Ryu, Hui Seong,Jang, Sung Hyun,Lee, Jung Ho,Lee, Jung Joon The Ecological Society of Korea 2014 Journal of Ecology and Environment Vol.37 No.4

This study was undertaken to suggest an effective fisheries resources management system by using stock assessment and potential yield analyses of crucian carp population in the mid-upper system of the Seomjin River. Fieldwork was conducted seasonally from 2008 to 2009 in the mid-upper system of the Seomjin River. The stock assessment was carried out by the swept area method and the potential yield was estimated by improved fisheries resource potential estimation system based on the Allowable Biological Catch. Also, the yield-per-recruit analysis was used to review the efficient management implication of the resource, Carassius auratus. As a result, the age at first capture ($t_c$) was estimated as 1.468 year, converted body length (BL) was 10.8 cm. Meaning the current fishing intensities, the instantaneous coefficient of fishing mortality (F) was $0.067year^{-1}$, and the yield-per-recruit analysis showed that the current yield per recruit was estimated to be 15.999 g with F and $t_c$. The instantaneous rate of fishing mortality that provides for Allowable Biological Catch ($F_{ABC}$) based on the current $t_c$ and F was estimated as $0.618year^{-1}$. Therefore, the optimum fishing intensities could be achieved at the higher fishing intensity for Carassius auratus. The calculated annual stock of C. auratus was estimated as 7,608 kg, and the potential yield was estimated as 343 kg with $t_c$ and F at the fixed current level. Using yield-per-recruit analysis, if F and $t_c$ were set at $0.618year^{-1}$ and 2 year, the yield per recruit and total allowable catch would be predicted to increase to 62 g and 2,531 kg by about 3.9 times and 7.3 times, respectively.

Genomics approach to develop molecular markers for targeted breeding of radish

Ji-Young Lee,Kook Hui Ryu,Jung-Hun Lee,Khushboo Rastogi,Goh Choi 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Radish is one of the most widely consumed vegetable crops in Korea. Root is the major part of radish supplied to the market, thus the size, shape, and quality of radish roots are main targets of breeding programs. Despite of the importance of this crop, the molecular breeding of radish is still in the rudimentary stage. In Golden Seed Project, we aim to establish the molecular breeding program of radish using genome-wide approaches. To this end, we selected inbred lines that have distinctive root traits such as yield, shape, disease resistance, and texture. Single nucleotide variation (SNV) among these lines will be identified based on the low coverage genome sequencing data. These SNVs can be used for finding genomic regions associated with root traits from segregating mapping populations which are also in the middle of development. Korean radish roots are harvested after being grown for only nine weeks. During that period, root biomass reaches to more than two kilograms. While investigating the root growth of radish inbred lines, we found that cytokinin contributes as a key growth regulator that promotes radial growth of radish roots. A difference in growth rates of two distinctive inbred lines was explained by the difference in response to cytokinin. Genes responsive to cytokinin are highly enriched in the cambium, the meristematic cell population that drives radial growth. For comprehensive understanding of genes that affect yields of radish roots, we turned to developing a tissue specific transcriptome data using laser capture microdissection. We expect that the compendium of genomics-based data will help establishing molecular breeding of radish at a fast track.

Distribution and current vegetation of Cakile edentula, an invasive alien species in Korea

Ryu, Tae-Bok,Choi, Dong-Hui,Kim, Deokki,Lee, Jung-Hyo,Lee, Do-Hun,Kim, Nam-Young The Ecological Society of Korea 2018 Journal of Ecology and Environment Vol.42 No.9

Background: Cakile edentula (Bigelow) Hook. is a successful invader that has been propagating globally. In Korea, Cakile edentula was found in 2008 for the first time, in the east coast of Korean peninsula. Based on site surveys conducted thereafter, Cakile edentula has propagated in the east coast from Goseong to Jeongdongjin, Gangneung and in the west coast of city of Hwaseong of Gyeonggi-do Province, and Taeangun County of Chungcheongnam-do Province. Results: Cakile edentula has infiltrated into four different vegetation communities (syntaxa) (Calystegio soldanellaeSalsoletum komarovii Ohba, Miyawakiet Tx.1967; Elymo-caricetum Kobomugi Miyawaki 1967; Carex pumila; and Calystegia soldanella) belonging to phytosociological classes of saltwort (barilla) and coastal glehnia. Cakile edentula competes with dominant species such as the saltwort (barilla) and beach morning glory, causing qualitative changes in species composition. It also affects local ecosystems through its competition with indigenous species, causing destruction of the traditional landscape. Conclusions: However, competition of Cakile edentula with indigenous species and the resulting replacement of such species are limitedly reported. Potential competitors with Cakile edentula have not been found yet. In Northeast Asia, qualitative changes realized in local ecosystems due to invasion of Cakile edentula are significantly noticeable. These necessitate proper controls for this invasive alien species to protect and preserve coastal dune areas.

Alteration of Gene Expression pattern in Human Placenta choriocarcinoma Cells by Exposed to Valproic acid.

Hui-Seong Kim(김희성),Youn-Jung Kim(김연정),Jae-Chun Ryu(류재천) 환경독성보건학회 2007 한국독성학회 심포지움 및 학술발표회 Vol.- No.-

[P5-20] Establishment of Tannin Enhancement Conditions for Development of High Quality Kiwi Wine

Hui-Hun Kim,Hyoun-Kook Cho,lg-Jo Kim,Eun-Jung Kim,Young-Hun Kim,Hyo-Hyung Lee,Jeong-Ok Lee,Chung-Ho Ryu. 한국식품영양과학회 2008 한국식품영양과학회 학술대회발표집 Vol.2008 No.10

A Systematic Review of Chuna Manual Therapy for Adolescent Idiopathic Scoliosis

Jung, Ga Hyeon,Lee, Hyun,Kong, Hae Jin,Ryu, Hwa Yeon,Ku, Yong Ho,Kang, Jae Hui Korean AcupunctureMoxibustion Medicine Society 2019 Korean Journal of Acupuncture Vol.36 No.3

This study analyzed randomized controlled trials (RCTs) and case studies investigating Chuna manual therapy and variations of this term, for adolescent idiopathic scoliosis. On June $15^{th}$, 2019, 6 online databases were used to retrieve studies. A total of 527 articles were retrieved, and 14 RCTs and 20 case studies were selected for review. Typically, the frequency of Chuna therapy was 1-2 times/week. The most common period of treatment was 12 months in RCTs and 3-6 months in case studies. Cobb's angle was the most frequent evaluation index used (11 RCTs and 20 case studies). In control groups, brace treatment was used in 8 RCTs. In 6 RCTs and 20 case studies, Cobb's angle significantly decreased after Chuna therapy, and in 4 RCTs, Chuna therapy was as effective as brace treatment, with no significant difference between groups. Adverse events were not reported except for minor reactions in only 3 case studies. This review suggested that Chuna therapy for adolescent idiopathic scoliosis was more advantageous than and as effective as brace treatment in most cases, although the risk of bias in 13 RCTs was unclear.

Allergenicity Change of Soybean Proteins by Thermal Treatment Methods

Hui-Gyeong Seol(설희경),Yu-Jin Ko(고유진),Eun-Jung Kim(김은정),Gyeong-Lan Lee(이경란),Do-Gyeong Kim(김도경),Jeong-Ok Lee(이정옥),Kang-Mo Ahn(안강모),Chung-Ho Ryu(류충호) 한국생명과학회 2012 생명과학회지 Vol.22 No.4

콩은 우리나라에서 과민성 알레르기를 일으키는 대표적인 식품 중의 하나로, 식품으로 섭취 시에 가열 및 발효가공을 통한 형태로 섭취한다. 이에 본 연구에서는 콩에 알레르기 반응을 일으키는 7명의 환아 혈청과 1명의 정상인 혈청을 이용하여 열처리 방법에 따른 품종별 콩(대풍, 대원, 태광)의 알레르기성에 미치는 영향을 조사하였다. 단백질을 추출하여 SDS-PAGE, immunoblotting 및 ELISA 방법을 통하여 반응성을 조사한 결과, SDS-PAGE상에서 열처리하지 않은 세가지 품종의 경우 9-76 kDa 위치에서 다양한 단백질 밴드를 보였는데 특히 9, 21, 34,52, 72 그리고 76 kDa의 단백질들은 각각 LTP, Kunits trypsin inhibitor, Gly m Bd 30K, β-conglycinin의 β-subunit, α-subunit와 α’-subunit로 주요한 콩 알레르겐으로 알려져 있다. 반면에 볶은 콩, 발효한 콩에서는 35kDa 이하로 완전히 분해되어 열처리 방법을 통해 단백의 항원성이 줄어드는 것을 확인할 수 있었다. IgE immunoblotting 을 통한 세 가지 품종의 볶은 콩과 콩 알레르기 환아 혈청과의 반응에서는 공통적으로 38-40kDa과 10-15 kDa에서 단백질 밴드를 보였으나 발효한 콩에서는 대부분 반응성이 약하거나 나타나지 않았다. ELISA 결과, immunoblotting 분석과 동일하게 대부분의 환아 혈청과 반응시에 볶은 콩과 발효한 콩에서 비교적 낮은 수치를 보였다. 결론적으로 콩에 존재하는 알레르겐 단백질은 열처리와 발효 미생물이 분비하는 단백질 분해효소에 의해 대부분의 환아에서 콩 단백질과의 반응성이 약화되는 것으로 사료된다. Soybean is one of the most common food materials causing food hypersensitivity reactions in Korea. In this study, we have investigated the effect of roasting and fermentation on the allergenicity of soybean. Three kinds of soybean (Daepung, Daewon, and Taegwang) were prepared as raw, roasted, and fermented by Bacillus subtilis GSK 3580, and then their proteins were extracted. The proteins were separated using SDS-PAGE, and the detection of IgE specific to soybean proteins was performed by immunoblotting using 7 sera of soybean allergy patients and non-allergic control individuals. Serum specific IgE to soybean was measured by ELISA. The SDS-PAGE of raw soybean proteins showed various-sized bands ranging from 9 to 76 kDa, which are known as major allergens. In particular, 9, 21, 34, 52, 72, and 76 kDa proteins are known as LTP, Kunits trypsin inhibitor, Gly m Bd 30K, β-subunit, α-subunit, and α’-subunit of β-conglycinin, respectively; these are major allergens in soybean. In contrast, only peptides of less than 35 kDa were found in roasted and fermented soybeans. IgE immunoblot analysis of three roasted species of soybeans commonly detected at 38-40 kDa and 10-15 kDa. The protein bands in fermented soybean showed very weak signals or were not detected. In addition, the reactivity of most patients’ sera to soybean was decreased after roasting and fermentation. With these results, it may be concluded that the allergenicity of soybeans is reduced by the roasting and fermentation processes. It is supposed that allergenic proteins in soybean were degraded by heat treatment methods and proteolytic enzymes were secreted from fermenting microorganisms.

Systemic Approaches Identify a Garlic-Derived Chemical, Z-ajoene, as a Glioblastoma Multiforme Cancer Stem Cell-Specific Targeting Agent

Jung, Yuchae,Park, Heejoo,Zhao, Hui-Yuan,Jeon, Raok,Ryu, Jae-Ha,Kim, Woo-Young Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.7

Glioblastoma multiforme (GBM) is one of the most common brain malignancies and has a very poor prognosis. Recent evidence suggests that the presence of cancer stem cells (CSC) in GBM and the rare CSC subpopulation that is resistant to chemotherapy may be responsible for the treatment failure and unfavorable prognosis of GBM. A garlic-derived compound, Z-ajoene, has shown a range of biological activities, including anti-proliferative effects on several cancers. Here, we demonstrated for the first time that Z-ajoene specifically inhibits the growth of the GBM CSC population. CSC sphere-forming inhibition was achieved at a concentration that did not exhibit a cytotoxic effect in regular cell culture conditions. The specificity of this inhibitory effect on the CSC population was confirmed by detecting CSC cell surface marker CD133 expression and biochemical marker ALDH activity. In addition, stem cell-related mRNA profiling and real-time PCR revealed the differential expression of CSC-specific genes, including Notch, Wnt, and Hedgehog, upon treatment with Z-ajoene. A proteomic approach, i.e., reverse-phase protein array (RPPA) and Western blot analysis, showed decreased SMAD4, p-AKT, 14.3.3 and FOXO3A expression. The protein interaction map (http://string-db.org/) of the identified molecules suggested that the AKT, ERK/p38 and $TGF{\beta}$ signaling pathways are key mediators of Z-ajoene's action, which affects the transcriptional network that includes FOXO3A. These biological and bioinformatic analyses collectively demonstrate that Z-ajoene is a potential candidate for the treatment of GBM by specifically targeting GBM CSCs. We also show how this systemic approach strengthens the identification of new therapeutic agents that target CSCs.

6-Arylamino-5,8-quinazolinediones as potent inhibitors of endothelium-dependent vasorelaxation

Ryu, Chung-Kyu,Shin, Keun-Hwa,Seo, Ji-Hui,Kim, Hwa-Jung 梨花女子大學校 藥學硏究所 2002 藥學硏究論文集 Vol.- No.11

6-(Substituted-phenyl)amino-5,8-quinazolinediones (3) were synthesised by regioselective substitution of 5,8-quinaaolinedione(5) with appropriate aflamines in the presence of Ce(Ⅲ) ions. All synthesised 5,8-quinazolinediones 3 showed a potent andefficacious inhibitory effect on the acetylcholine (ACh)-induced vasorelaxation of rat aorta with the endothelium. The quinones3, at a low concentration of 0.1 μM, reduced the maximal response with increase of EC_50 values for ACh. The results indicatethat quinones 3 are potent inhibitors of endofhelium-dependent vasorelaxation.

7-(SUBSTITUTED-PHENYL)AMINO-5,8-QUINAZOLINEDIONES : POTENT INHIBITORS OF ENDOTHELIUM-DEPENDENT VASORELAXATION

Ryu, Chung-Kyu,Shin, Keun-Hwa,Seo, ji-Hui,Kim, Hwa-Jung 梨花女子大學校 藥學硏究所 2001 藥學硏究論文集 Vol.- No.10

7-(Substituted-phenyl)amino-5, 8-quinazolinediones(4) were synthesized by the substitution of 7-methoxy-5, 8-quinazolinedione(5) with arylamines. All synthesized5, 8-quinazolinediones 4 showed a potent and efficacious inhibitory efffect on the acetylchooline(ACh)-induced vasorelaxation of rat aorta with the endothelium. The quinones, at a low concentratiin of 0.1㎛, reduced the maximal response with an increase of EC_50 values for ACh. The results indicate that quinones 4 are potent inhibitors of endothelium-dependent vasorelaxation.