Effects of Black Raspberry Extract on Choline-Induced Inflammation in High-Fat Diet-Fed Female SD Rats

Juhee Ryu,Taehwan Lim,Kiuk Lee,Keum Taek Hwang 한국식품영양과학회 2018 한국식품영양과학회 학술대회발표집 Vol.2018 No.10

Urinary Angiotensinogen in addition to Imaging Classification in the Prediction of Renal Outcome in Autosomal Dominant Polycystic Kidney Disease

박혜인,Kim Juhee,조아진,Kim Do Hyoung,이영기,Ryu Hyunjin,김현숙,Oh Kook-Hwan,오윤규,Hwang Young-Hwan,Lee Kyu-Beck,Kim Soo Wan,Kim Yeong Hoon,Lee Joongyub,안규리,KNOW-CKD Investigators Group 대한의학회 2020 Journal of Korean medical science Vol.35 No.22

Background: Intrarenal renin-angiotensin system (RAS) is known to play the major role in the development of hypertension and renal progression in autosomal dominant polycystic kidney disease (ADPKD). Urinary angiotensinogen to creatinine ratio (AGT/Cr) was suggested as a novel biomarker to reflect intrarenal RAS activity. This study was performed to evaluate urinary AGT/Cr as a predictive biomarker for renal function decline in addition to imaging classification in a prospective ADPKD cohort. Methods: From 2011 to 2016, a total of 364 ADPKD patients were enrolled in the prospective cohort called the KoreaN Cohort Study for Outcomes in Patients With Chronic Kidney Disease (KNOW-CKD). Among them, a total of 207 subjects in chronic kidney disease stage 1–4 with baseline urinary AGT and total kidney volume and subsequent renal function follow-up data over more than 1 year were included in the analysis. Patients were defined as slow progressors (SP) if they are classified as 1A or 1B by imaging classification whereas rapid progressors (RP) if they are classified as 1C–1E. Patients were divided according to AGT/Cr quartiles and annual estimated glomerular filtration rate (eGFR) slope was compared among highest quartile (hAGT group) and the rest of quartiles (lAGT group). Patients were divided into 4 groups to evaluate the predictive value of urinary AGT/Cr in addition to imaging classification: SP/lAGT, SP/hAGT, RP/lAGT, and RP/hAGT. The Cox regression model was used to evaluate the hazard ratio (HR) between groups. Results: The mean age was 45.9 years and 88.9% had hypertension. Baseline eGFR was 79.0 ± 28.4 mL/min/1.73 m2 and median height-adjusted total kidney volume was 788.2 (471.2;1,205.2) mL/m. The patients in the hAGT group showed lower eGFR (72.4 ± 24.8 vs. 81.1 ± 29.2 mL/min/1.73 m2 , P = 0.039), lower plasma hemoglobin (13.0 ± 1.4 vs. 13.7 ± 1.6 g/dL, P = 0.007), higher urinary protein to creatinine ratio (0.14 [0.09, 0.38] vs. 0.07 [0.04, 0.12] g/g, P = 0.007) compared to the lAGT group. The hAGT group was an independent risk factor for faster eGFR decline after adjusting for gender, RP, baseline eGFR, and other known risk factors. During median follow-up duration of 4.6 years, a total of 29 renal events (14.0%) occurred. The SP/hAGT group showed significantly higher risk of developing renal outcome compared to SP/lAGT group (HR, 13.4; 95% confidence interval, 1.282–139.324; P = 0.03). Conclusion: Urinary AGT/Cr can be a useful predictive marker in the patients with relatively small ADPKD. Various biomarkers should be considered to define RP when implementing novel treatment in the patients with ADPKD.

Effects of Consumption of Black Raspberry Extract on Hepatic Cholesterol and Bile Acid Metabolism in Female SD Rats Fed High-Fat Diet and High-Choline Water

Taehwan Lim,Juhee Ryu,Kiuk Lee,Keum Taek Hwang 한국식품영양과학회 2018 한국식품영양과학회 학술대회발표집 Vol.2018 No.10

과학 커뮤니케이션에 있어 전문가의 역할과 기능, 대중 참여에 대한 탐색적 연구: 뇌과학자들의 인터뷰 중심 분석

김수진(Soojin Kim),엄주희(Juhee Eom),엄주희(Young-Joon Ryu) 한국헬스커뮤니케이션학회 2024 헬스커뮤니케이션연구 Vol.23 No.2

본 연구의 목적은 과학기술의 발전과 더불어 중요성이 커진 위험과 과학 커뮤니케이션에 대한 전문가 의견을 통해 뇌과학 커뮤니케이션에 대한 대중 참여의 의미를 고찰하는데 있다. 이를 위해 뇌과학자 10명을 대상으로 초점 집단을 구성하여 온라인 인터뷰를 진행했다. 인터뷰는 첫째, 뇌과학자들이 연구수행과 관련하여 경험한 커뮤니케이션의 문제, 둘째, 뇌과학자가 바라본 우리나라 과학 커뮤니케이션의 현재, 셋째, 대중 관심 제고와 참여 도모를 위한 뇌과학자들의 방법론적 제안에 대한 질문으로 구성했다. 연구 결과, 뇌과학자들은 대중에 대한 전문지식 전달의 문제, 연구결과의 순기능과 의도하지 않은 효과의 문제를 지적했고, ‘언론속의 과학보도’ 즉, 미디어를 통한 과학기술에 대한 이해는 연구결과의 긍정적 측면 뿐만 아니라 부정적 측면에 대한 고려 또한 필요함을 나타냈다. 마지막으로, 신기술의 사회적 적용에 대한 대중의 관심 제고와 참여 도모를 위해서는 과학자, 인문학자, 사회과학자, 법학자 등 사회적 영향에 대한 논의가 가능한 전문가 집단의 협업이 필요함을 제안했다. 이 연구는 탐색적 연구로서 향후 과학 커뮤니케이션 분야에서 다루어야 할 연구주제에 대한 인사이트를 제공하고, 위험과 과학 커뮤니케이션의 통합적 관점에서 대중 참여의 중요성을 고찰한 것에 의미가 있다. In an era of rapid scientific and technological advancement, public engagement in risk and science communication has become increasingly crucial. This study delves into the multifaceted concept of public engagement, exploring its significance in forging social consensus and agreement. Through online interviews with a focus group of 10 neuroscientists, a field at the forefront of scientific communication, the study examines the various dimensions of communication challenges faced by neuroscientists. Further, we sought to find out about the current state of science communication in Korea as perceived by neuroscientists. Additionally, the study explores the methodological discussions necessary for engaging the public prior to the societal application of brain-based technologies. The findings reveal internal conflicts among neuroscientists regarding the legitimacy of research methods and the scientific basis of research results. They also highlight the polarization between patient treatment and researcher-driven research. Moreover, neuroscientists expressed concerns about the potential misuse of research results in social applications, emphasizing the need to consider both the positive and negative aspects of scientific breakthroughs. The study concludes by advocating for collaborative efforts between scientists, humanities scholars, social scientists, lawyers, and other experts to foster public interest and participation in the social application of new technologies. This exploratory study contributes to a comprehensive understanding of public engagement from the integrated perspective of risk and science communication.

망막 혈관 질환에서 원형 RNA의 작용 기전 및 RNA 치료제로서의 가능성

김현종(Hyunjong Kim),류주희(Juhee Ryu) 대한약학회 2023 약학회지 Vol.67 No.6

Disruption of Microtubules Sensitizes the DNA Damage-induced Apoptosis Through Inhibiting Nuclear Factor κB (NF-κB) DNA-binding Activity

Lee, Hyunji,Jeon, Juhee,Ryu, Young Sue,Jeong, Jae Eun,Shin, Sanghee,Zhang, Tiejun,Kang, Seong Wook,Hong, Jang Hee,Hur, Gang Min The Korean Academy of Medical Sciences 2010 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.25 No.11

<P>The massive reorganization of microtubule network involves in transcriptional regulation of several genes by controlling transcriptional factor, nuclear factor-kappa B (NF-κB) activity. The exact molecular mechanism by which microtubule rearrangement leads to NF-κB activation largely remains to be identified. However microtubule disrupting agents may possibly act in synergy or antagonism against apoptotic cell death in response to conventional chemotherapy targeting DNA damage such as adriamycin or comptothecin in cancer cells. Interestingly pretreatment of microtubule disrupting agents (colchicine, vinblastine and nocodazole) was observed to lead to paradoxical suppression of DNA damage-induced NF-κB binding activity, even though these could enhance NF-κB signaling in the absence of other stimuli. Moreover this suppressed NF-κB binding activity subsequently resulted in synergic apoptotic response, as evident by the combination with Adr and low doses of microtubule disrupting agents was able to potentiate the cytotoxic action through caspase-dependent pathway. Taken together, these results suggested that inhibition of microtubule network chemosensitizes the cancer cells to die by apoptosis through suppressing NF-κB DNA binding activity. Therefore, our study provided a possible anti-cancer mechanism of microtubule disrupting agent to overcome resistance against to chemotherapy such as DNA damaging agent.</P>

Alcoholic and Nonalcoholic Fatty Liver Disease and Incident Hospitalization for Liver and Cardiovascular Diseases

Chang, Yoosoo,Cho, Juhee,Cho, Yong Kyun,Cho, Ara,Hong, Yun Soo,Zhao, Di,Ahn, Jiin,Sohn, Chong Il,Shin, Hocheol,Guallar, Eliseo,Ryu, Seungho Elsevier 2020 Clinical gastroenterology and hepatology Vol.18 No.1

<P><B>Background & Aims</B></P> <P>We compared the associations of nonalcoholic fatty liver disease (NAFLD) and alcohol-associated fatty liver disease (AFLD) with risk of incident hospitalization for liver and cardiovascular diseases.</P> <P><B>Methods</B></P> <P>We collected data from the Kangbuk Samsung Health Study on 218,030 men and women in Korea who underwent a health examination from 2011 through 2016. Fatty liver disease (FLD) was detected by ultrasound during the initial examination. The Fibrosis-4 index was used to identify individuals with liver fibrosis. Participants were followed up for as long as 5.9 years and data on hospitalizations for liver and cardiovascular diseases were collected.</P> <P><B>Results</B></P> <P>The prevalence of NAFLD was 22.0% and the prevalence of AFLD was 6.4%. Over a median follow-up period of 4.2 years, we observed 51 and 1097 incident cases of liver disease– or cardiovascular disease–related hospitalizations, respectively. After adjustment for potential confounders, the multivariable-adjusted hazard ratios for liver disease–related hospitalization, comparing NAFLD and AFLD with the reference category (no excessive alcohol intake and no FLD), were 1.73 (95% CI, 0.76–3.96) and 5.00 (95% CI, 2.12–11.83), respectively. The corresponding hazard ratios for cardiovascular disease hospitalization were 1.20 (95% CI, 1.02–1.40) and 1.08 (95% CI, 0.86–1.34), respectively. Among participants with FLD, the risk of liver disease–related hospitalization increased with high Fibrosis-4 index scores, whereas the risk of incident cardiovascular disease did not.</P> <P><B>Conclusions</B></P> <P>In a large cohort study, we found an increased risk of liver disease–related hospitalizations for patients with NAFLD or AFLD, especially among those with Fibrosis-4 index scores. An increased risk of cardiovascular disease–associated hospitalization was observed in patients with NAFLD but not AFLD.</P>

Regulation of MDM2 E3 ligase-dependent vascular calcification by MSX1/2

권덕화,Choe Nakwon,신세라,Ryu Juhee,김낙성,Eom Gwang Hyeon,Nam Kwang-Il,Kim Hyung Seok,Ahn Youngkeun,김영국,Park Woo Jin,Mendrysa Susan M.,Kook Hyun 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

Vascular calcification increases morbidity and mortality in patients with cardiovascular and renal diseases. Previously, we reported that histone deacetylase 1 prevents vascular calcification, whereas its E3 ligase, mouse double minute 2 homolog (MDM2), induces vascular calcification. In the present study, we identified the upstream regulator of MDM2. By utilizing cellular models and transgenic mice, we confirmed that E3 ligase activity is required for vascular calcification. By promoter analysis, we found that both msh homeobox 1 (Msx1) and msh homeobox 2 (Msx2) bound to the MDM2 promoter region, which resulted in transcriptional activation of MDM2. The expression levels of both Msx1 and Msx2 were increased in mouse models of vascular calcification and in calcified human coronary arteries. Msx1 and Msx2 potentiated vascular calcification in cellular and mouse models in an MDM2-dependent manner. Our results establish a novel role for MSX1/MSX2 in the transcriptional activation of MDM2 and the resultant increase in MDM2 E3 ligase activity during vascular calcification.

Cloning of the Setd1b gene of Mus musculus, a novel histone methyl transferase target in the epigenetic therapy of cancers

Morishita, Masayo,Cho, Minju,Ryu, Juhee,Mevius, Damiaan E.H.F.,Di Luccio, Eric 경북대학교 농업과학기술연구소 2010 慶北大農學誌 Vol.28 No.-

The epigenetic therapy of cancers is emerging as an effective and valuable approach to both chemotherapy and the chemoprevention of cancer. The utilization of epigenetic targets that include histone methyltransferase (HMTase), Histone deacetylatase, and DNA methyltransferase, are emerging as key therapeutic targets. SET containing proteins such as the HMTase Setd1b has been found significantly amplified in cancerous cells. In order to shed some light on the histone methyl transferase family, we cloned the Setd1b gene from Mus musculus and build a collection of vectors for recombinant protein expression in E.coli that will pave the way for further structural biology studies. We prospect the role of the Setd1b pathway in cancer therapy and detail its unique value for designing novel anti-cancer epigenetic-drugs.

Correlation between gut microbiota and personality in adults: A cross-sectional study

Kim, Han-Na,Yun, Yeojun,Ryu, Seungho,Chang, Yoosoo,Kwon, Min-Jung,Cho, Juhee,Shin, Hocheol,Kim, Hyung-Lae Elsevier 2018 Brain, behavior, and immunity Vol.69 No.-

<P><B>Abstract</B></P> <P>Personality affects fundamental behavior patterns and has been related with health outcomes and mental disorders. Recent evidence has emerged supporting a relationship between the microbiota and behavior, referred to as brain-gut relationships. Here, we first report correlations between personality traits and gut microbiota. This research was performed using the Revised NEO Personality Inventory and the sequencing data of the 16S rRNA gene in 672 adults. The diversity and the composition of the human gut microbiota exhibited significant difference when stratified by personality traits. We found that personality traits were significantly correlated with diversity of gut microbiota, while their differences were extremely subtle. High neuroticism and low conscientiousness groups were correlated with high abundance of Gammaproteobacteria and Proteobacteria, respectively when covariates, including age, sex, BMI and nutrient intake, were controlled. Additionally, high conscientiousness group also showed increased abundance of some universal butyrate-producing bacteria including Lachnospiraceae. This study was of observational and cross-sectional design and our findings must be further validated through metagenomic or metatranscriptomic methodologies, or metabolomics-based analyses. Our findings will contribute to elucidating potential links between the gut microbiota and personality, and provide useful insights toward developing and testing personality- and microbiota-based interventions for promoting health.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Personality traits were correlated with gut microbiota composition. </LI> <LI> Gammaproteobacteria was increased in high neuroticism group. </LI> <LI> Low conscientiousness group showed increased abundance of Proteobacteria. </LI> <LI> The low conscientiousness group showed decreased abundance of Lachnospiraceae. </LI> </UL> </P>