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        An integrated strategy for production of four anthocyanin compounds from Ribes nigrum L. by deep eutectic solvents and flash chromatography

        Ping Kou,Yu-Fei Kang,Li-Tao Wang,Li-Jun Niu,Ying Xiao,Na Guo,Qi Cui,Yanyan Liu,Yu-Jie Fu 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.80 No.-

        This study explored an integrated strategy for extraction and preparative separation of four anthocyaninsfrom Ribes nigrum L. using DES-based microwave assisted extraction andflash chromatography. Theextraction yield of anthocyanins reached 2.03 mg/g after optimization. An efficient preparativeseparation process of four main anthocyanins by macroporous resin andflash chromatography wasestablished afterwards. The yields of D3G, D3R, C3G and C3R reached 0.32 mg/g, 0.47 mg/g, 0.18 mg/g and0.43 mg/g with the purities of more than 95%, respectively. The developed procedure represents anefficient and convenient alternative for the systematic utilization of natural products from plantmaterials.

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        Effect of a Mangosteen Dietary Supplement on Human Immune Function: A Randomized, Double-Blind, Placebo-Controlled Trial

        Yu-Ping Tang,Peng-Gao Li,Miwako Kondo,Hong-Ping Ji,Yan Kou,Boxin Ou 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.4

        The effect of a mangosteen product containing multivitamins and essential minerals was tested on immune function and well-being in healthy adults. A randomized, double blinded, placebo-controlled study was conducted in 59 healthy human subjects (40–60 years old). Changes from baseline immune function were measured after a 30-day consumption of the mangosteen product and the placebo. The subjects' self-appraisal of their health status was also surveyed. A xanthone-rich mangosteen product intake increased mean values for peripheral T-helper cell frequency (P=.020) and reduced the serum C-reactive protein concentration (P=.014). Increases in peripheral CD4/CD8 double-positive (DP) T-cell frequency and serum complement C3, C4, and interleukin (IL)-1α concentrations were significantly higher in the experimental group than in the placebo group (DP, P=.038; C3, P=.017; C4, P=.031; IL-1α, P=.006). At the end of study, serum IL-1α and IL-1β concentrations in the study group were significantly higher than that in the placebo group (IL-1α, P=.033; IL-1β, P=.04). Furthermore, more participants in the experimental group reported greatly improved overall health status compared with participants receiving placebo (P=.001). The results indicated that the intake of an antioxidant-rich product significantly enhanced immune responses and improved the subject's self-appraisal on his or her overall health status.

      • KCI등재

        Effect of a Mangosteen Dietary Supplement on Human Immune Function: A Randomized, Double-Blind, Placebo-Controlled Trial

        Tang, Yu-Ping,Li, Peng-Gao,Kondo, Miwako,Ji, Hong-Ping,Kou, Yan,Ou, Boxin The Korean Society of Food Science and Nutrition 2009 Journal of medicinal food Vol.12 No.4

        The effect of a mangosteen product containing multivitamins and essential minerals was tested on immune function and well-being in healthy adults. A randomized, double blinded, placebo-controlled study was conducted in 59 healthy human subjects (40.60 years old). Changes from baseline immune function were measured after a 30-day consumption of the mangosteen product and the placebo. The subjects' self-appraisal of their health status was also surveyed. A xanthone-rich mangosteen product intake increased mean values for peripheral T-helper cell frequency (P=.020) and reduced the serum C-reactive protein concentration (P=.014). Increases in peripheral CD4/CD8 double-positive (DP) T-cell frequency and serum complement C3, C4, and interleukin (IL)-$1{\alpha}$ concentrations were significantly higher in the experimental group than in the placebo group (DP, P=.038; C3, P=.017; C4, P=.031; IL-$1{\alpha}$, P=.006). At the end of study, serum IL-$1{\alpha}$ and IL-$1{\beta}$ concentrations in the study group were significantly higher than that in the placebo group (IL-$1{\alpha}$, P=.033; IL-$1{\beta}$, P=.04). Furthermore, more participants in the experimental group reported greatly improved overall health status compared with participants receiving placebo (P=.001). The results indicated that the intake of an antioxidant-rich product significantly enhanced immune responses and improved the subject's self-appraisal on his or her overall health status.

      • KCI등재

        Release Mechanism and pH Responsiveness of Starch-Based Polymers

        Zongliang Kou,Detian Dou,Jiayi Zhu,Yinyin Mai,Herui Yi,Lihong Lan,Ping Lan 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.11

        Amphiphilic polymer carriers (PEG–St–R) were prepared from cassava starch and their pH response was investigated. First, hydrophobic tapioca starch polymer (St–R) was prepared with octyl acyl as the hydrophobic group. The hydrophilic group polyethylene glycol (mPEG) was then introduced into the polymer by esterification to produce amphiphilic tapioca starch polymer (PEG–St–R). Its self-assembly behavior was characterized using fluorescent probes. The morphology of PEG–St–R was investigated by transmission electron microscopy (TEM). Loading of the anti-cancer drug curcumin was used to assess the delivery and slow-release performance of the amphiphilic tapioca starch polymer. Cumulative drug release was explored at various pH conditions, with the greatest release from drug-loaded micelles being observed under acidic conditions and stable in a neutral environment. These results provide a theoretical basis for the preparation of pH-responsive nanomicelle carriers, and a platform for the preparation of novel amphiphilic starch-based polymers.

      • KCI등재

        An integrated strategy for extraction and pre-concentration of four astragalosides from Radix Astragali by a formulated surfactant aqueous system

        Suixin Wang,Jiao Jiao,Xinyu Wang,Qingyan Gai,Ping Kou,Wenjing Xu,Meng Luo,Chunjian Zhao,Yu-Jie Fu 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.70 No.-

        Integrated and environmentally friendly methods for extraction and enrichment of natural products havegained increasing interests. The proposed preparation process provides the possibility that extractionand pre-concentration for astragalosides from Radix Astragali roots can be conducted with the surfactantaqueous system in one single procedure. The formulated surfactant system composing of 2% TX-114 assurfactant and 0.03% (w/v) of Gemini 16-5-16 as co-surfactant showed higher extraction efficiency withultrasonic-assisted method and four astragalosides could be enriched by the cloud point phenomenon ofsurfactant. This work represents a valuable alternative for the eco-friendly extraction and enrichment ofsecondary metabolites from plants.

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