포도당과 이소프로테레놀에 의한 시상하부의 소마토스타틴 분비가 갑상선자극호르몬방출호르몬에 의한 갑상선자극호르몬에 미치는 효과

박철영,양인명,오승준,김덕윤,우정택,김성운,김진우,김영설,최영길 대한내분비학회 2000 Endocrinology and metabolism Vol.15 No.4·5

단기배양을 통한 말초혈액 CD34 양성세포의 체외증폭

박상준,김철희,배광봉,김현수,김종숙,윤환중,조덕연,김삼용 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.1

Background: It is suggested that clinical practice in the areas of bone marrow transplantation and gene therapy might rely on the ex vivo expansion of hematopoietic stem/progenitor cells. However, the condition for ex vivo expansion of hematopoietic progenitor cells is not well established. The authors pursued a series of experiments to define the proper conditions for the expansion of hematopoietic cells in the short-term liquid suspension culture of mobilized peripheral blood CD34+ cells. Methods: 1.0ml cultures were initiated with 9×10^3 PB CD34+ cells, which were isolated from PB mononuclear cells (MNCs) by high-gradient cell sorting, in 12 well plates with the various combinations of hematopoietic growth factors(HGF). The following recombinant human HGFs were used: stem cell factor(SCF) 100ng/ml, granulocyte colony-stimulating factor(G-CSF) 100ng/ml, GM-CSF(granulocyte, macrophage colony-stimulating factor) 100ng/ml, interleukin-1 beta(IL-1B) 1ng/ml, interleukin-3(IL-3) 20ng/ml, interleukin-6 (IL-6) 100ng/ml. At the end of culture, colony-forming cells were evaluated by semisolid clonogenic assay. Results: 1) Using the high-gradient magnetic sorting system, CD34^+ cells were isolated with a yield of 40 3% 2) In 7 day culture of PB CD34^+ cells(9×10^3 cells), nucleated cells expanded mean 10×10^3(range, 9 to 20×10^3) with the addition of SCF alone, 35×10^3(range, 10 to 60×10^3) with SCF plus G-CSF plus GM-CSF, and 130×10^3(range, 40 to 300×10^3) with the combination of SCF, G-CSF, IL-1, IL-3, IL-6, GM-CSF. In 14 day culture, nucleated cells expanded 10×10^3 to 1,860×10^3 with combination of human hematopoietic growth factors. 3) In 10 day culture without medium change of PB CD34^+ cells, CFU-GM numbers expanded 16. 5 fold(range, 7 to 59 fold) with the addition of SCF plus G-CSF plus Il-1 plus IL-3, 31.3 fold(range, 20.5 to 101.1 fold) with the combination of SCF, G-CSF, IL-1, IL-3, GM-CSF. In 14 day culture with or without medium change of PB CD34^+ cells was inferior to 10 day culture for CFU-GM expansion. 4) There was no significant difference for CFU-GM expansion between five growth factors(SCF,G-CSF,IL-1,IL-3,GM-CSF) and six growth factors(five growth factors plus IL-6). Conclusion: The authors could confirm that short-term suspension culture of peripheral blood CD34+ cells could expand hematopoietic progenitor cells. Ten-day culture with medium change of CD34+ cells with the addition of five growth factors, i.e. SCF, G-CSF, IL-1B, IL-3, and GM-CSF, might be the most efficient in this system.

삼변수 kappa 분포를 적용한 매년 최대연속강우량 계열의 빈도해석

박상덕,이원환 연세대학교 산업기술연구소 1991 논문집 Vol.23 No.2

The total depths of a rainfall event may be important in planning of flood control. This study is to analyse the applicability of three parameter kappa distribution for annual maximum series of continuous rainfall at Seoul, Incheon, Busan, and Kwangju meteorological station and to compare to three parameter lognormal distribution, extremal type 1 distribution, Pearson type 3 distribution, and log-Pearson type 3 distribution. As results of this study annual maximum series of continuous rainfall are random data and are fitted by three parameter kappa distribution. For large return period the probable continuous rainfall depths by three parameter kappa distribution are more than those by other distribution, for small return period the rainfall depths are less. The probable continuous rainfall depths by extremal type 1 distribution are computed less than by other distribution. The type of best fitted probable distribution for a rainfall data must be chosen by considering the X² value and the probable rainfall depths for large return period.

短時間 確率計劃降雨波型의 推定

朴相德,金弘泰,孫廣翼 강릉대학교동해안지역연구소 1993 東海岸硏究 Vol.4 No.3

未計測 中小規模 河川流域에서 降雨量은 水工計劃의 중요한 資料이다. 短時間 計劃降雨波型을 推定하기 위하여 江陵地方의 每年最大 120分 降雨事象의 時刻別 강우량 時系列에 5乘根 變數變換 正規分布를 적용할 수 있음을 확인하고, 再現期間別 無次元 確率降雨波型을 提示하였다. 강릉지방의 재현기간에 따른 無次元 確率計劃降雨波型은 평균적으로 볼 때 1四分位에 集中된 單一 尖端降雨波型을 나타내고 있으나, 재현기간이 증가함에 따라 1사분위와 3사분위에 2개의 尖端降雨를 갖고 降雨持續期間의 後半部에 강우량이 집중되는 傾向을 나타낸다. The rainfall depths are important data for hydraulic engineering projects in an ungaged small watershed. To estimate probability rainfall patterns for short duration, annual maximum rainfall series for duration of 120 mimutes of Kangnung site were fitted in the 5-root transformed normal distribution. Dimensionless probability rainfall patterns are sugessted in each return period. These show the simple peak in first quartile for lower return period and the trend of concentration at the first part of rainfall duration. However, for upper return period that is changed into double peak in first and third quartile and into trend of concentration at the later part of rainfall duration.

도시유역 배수 관망체계의 계량형태학적 분석

박상덕,김문모,이원한 연세대학교 산업기술연구소 1991 논문집 Vol.23 No.2

The objective of this study is to analyse the validity of urban drainage systems. Morphometric theory is applied to storm sewer systems of Seoul city for analysing this drainage network morphometrically. As the result, Horton's stream order method is applicable to the urban drainage systems and the validity of the drainage systems is judged and also applying this result to ungaged basin. Same as the rainfall intensity, duration and bifurcation ratio in case of bad drainage systems, the peak flow per unit drainage area is smaller than that of good drainage systems. So, if the bifurcation ratio is large, the flood control is advantageous. Because the shape of the basin effects on the runoff hydrograph, successive study considered to morphometric characteristics of the basin is needed.

정상과 갑상선 종양조직에서 사람 IGF-I 유전자의 발현

김성운,장현하,박상미,김덕윤,우정택,양인명,김진우,김영설,김광원,고석환,홍성화,최영길 경희대학교 유전공학연구소 1993 遺傳工學論文集 Vol.5 No.-

Many of the growth-promoting properties of growth hormone(GH) are mediated by insulin-like growth factor-I(IGF-I), a highly conserved circulating 70-amino acid peptide. Recent studies have shown that multiple mechanisms influence IGF-I gene expression, including transcription from two promoters, alternative RNA splicing, and variable polyadenylation. In thyroid tissue, thyroid stimulating hormone(TSH) and IGF-I are the most possible candidates for follicular cell proliferation and hypertrophy. Actually IGF-I had autocrine and paracrine effect for tissue growing. We prepared thyroid tumor tissue mRNAs using single step method for detecting IGF-I levels according to different tissues, i.e., thyroid adenoma or papillary thyroid carcinoma. We used Northern blot analysis for IGF-I mRNA and RNase protection assay (RPA) for IGF-I transcription start sites. For Northern blot, we used whole human IGF-I cDNA as a DNA probe and for RPA, we used IGF-I exon 1 containing noncoding promoter 1 as a riboprobe. We got good RNA bands from Northern blot analysis around 1 kb (IGF-IA) and 7.5 kb (IGF-IB) region. To clarify the amount of both IGF-IA and IB mRNAs, we measured autoradiographied signal of IGF-I mRNAs bands using densitometer. In IGF-IA signals, there's no change among liver and thyroid tissues, but in case of IGF-IB mRNA bands, the signal was markedly increased in thyroid carcinoma tissues than that of normal thyroid tissue (85% vs 14%). In the study of RPA, all thyroid tissues used the same transcription start sites as those of liver's. We concluded that that this different regulation of IGF-I mRNA was originated from tissue specificity. That meant some tissue specific transcription factor/s were related to tissue IGF-I expression.

토양에서 Napropamide의 흡착과 이동

라덕관,박상숙,김유봉,허동균 순천대학교 공업기술연구소 1998 工業技術硏究所論文集 Vol.12 No.-

This study was to investigate the adsorption and movement of Napropamide in soil. In order to find out the adsorption and movement characteristics of Napropamide in soils of sand loam (soil A), silt clay(soil B), and loam (soil C), batch and column tests were performed with respect to shaking time variation, adsorption isotherm, and breakthrough curve. The shaking time to reach the adsorption equlibrium of Napropamide was 12 hours. Freundlich isotherm was fit for the adsorption of Napropamide in soils. soil B had the highest adsorption coefficient of Napropamide, soil Ca and B followed at adsorption isotherm. The slope of breakthrough curve of soil A had the steep, soil C and soil B followed at the adsorption curve. The moving velocity of Napropamide in soils was the lowest of soil B because of its highest adsorption of Napropamide.

사이토카인과 Lipopolysaccharide 자극에 의한 RAW 264.7 세포주의 nitric oxide 생성

김영덕,전창덕,이병순,이복수,박석돈,백상기,정헌택 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-

Macrophages have been implicated as a major class of effector in the host response to neoplasia. Cells of the monocyte-macrophage lineage are known to exhibit tumoricidal activity following stim-ulation by γ-interferon, tumor necrosis factor, BCG and bacterial products such as lipopoly-saccharide (LPS). While the mechanism involved remain obscure, the generation of reactive nitrogen intermediate (RNI) by activated macrophages is considered a maior participant in mediating the tumorstatic effect. But much of what is known about the induction and release of RNI has been elucidated by using freshly isolated cells from blood and other tissues of experimental animals. In this study, we used a murine macrophage cell line, RAW 264.7, and found that these cells showed above 99% positive of pan macrophage marker by immunohistochemical staining. These cells could produce nitric oxide (NO), when incubated with γ-IFN or poly I:C. Incubation of RAW 264.7 cells with γ-IFN for 48 hous in the presence of LPS agumented NO release in a dose dependent manner. Whereas, treatment of anti-TNE-α antibody or antisense TNF-α oligodeoxynucleotide inhibited the release of NO_2 by γ-IFN plus LPS activated macrophages. The production of NO was also inhibited reversibly or irreversibly by N^GMMA,NAA,arginase or DPI. Thease data suggest that RAW 264.7 cell line may be useful for the in vitro evalulation of biological response modifiers as well as the study of signal pathway of NO release by macrophages.

염증성 근섬유모세포종의 증례보고

은상아,박혁,차인호,김현실,정호걸,김기덕,박창서 대한구강악안면방사선학회 2006 Imaging Science in Dentistry Vol.36 No.3

The inflammatory myofibroblastic tumor (inflammatory pseudotumor) is a rarely occurring soft tissue lesion of unknown etiology. It can be of any location, but commonly it is found in lungs. It has been considered as a nonneoplastic reactive inflammatory lesion, but nowadays, confusion and dispute about its character is increasing due to its high recurrence rate and metastasis. We present a patient who had been diagnosed with an inflammatory pseudotumor in the right maxilla area, 1 year before visiting our hospital. After that, her pain and swelling did not resolved and she visit our hospital. On radiographic examination, aggressively infiltrative growth of the lesion with destruction of adjacent bony structure was noted. We found unusual aggressiveness of the inflammatory myofibroblastic tumor of the head and neck region. Because the typical behavior of the inflammatory myofibroblastic tumor is not defined yet, we recommend the surgical excision of the lesion and close follow-up.

구내 표준 방사선사진을 이용한 구내방사선촬영기의 선질 평가

이상섭,권혁락,심우현,오승현,이지연,전국진,김기덕,박창서 대한구강악안면방사선학회 2000 Imaging Science in Dentistry Vol.30 No.3

Purpose : This study was to evaluate the beam quality of intraoral X-ray equipments used at Yonsei University Dental Hospital (YUDH) using the half value layer (HVL) and the characteristic curve of intraoral standard X-ray film. Materials and Methods : The study was done using the intraoral X-ray equipments used at each clinical department at YUDH. Aluminum filter was used to determine the HVL. Intraoral standard film was used to get the characteristic curve of each intraoral X-ray equipment. Results : Most of the HVLs of intraoral X-ray equipments were higher than the least recommended thickness, but the REX 601 model used at the operative dentistry department and the X-707 model used at the pediatric dentistry department had HVLs lower than the recommended thickness. The slopes of the characteristic curves of films taken using the PANPAS 601 model and REX 601 model at operative dentistry department, the X-705 model of prosthodontic dentistry department, and the REX601 model at the student clinic were relatively low. Conclusion : HVL and the characteristic curve of X-ray film can be used to evaluate the beam quality of intraoral X-ray equipment. In order to get the best X-ray films with the least radiation exposure to patients and best diagnostic information in clinical dentistry, X-ray equipment should be managed in the planned and organized fashion. (Korean J Oral Maxillofac Radiol 2000; 30: 183-188)