Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

응급의료센터로 내원한 헬기 후송 환자의 현황 및 분석

박진,조남수,조수형 대한응급의학회 1996 대한응급의학회지 Vol.7 No.2

Our hospital is only emergency medical center that possess the landing zone of helicopter in chon-nam, Korea. An analysis of the present condition of helicopter-transport patient, was performed in order to pursuit and extend the availability of air transport in the future. The result were as follows; 1) The investigated in the present condition by yearly: 2 cases in 1991, 5 cases in 1992, 4 cases in 1993, 9 cases in 1994, 5 cases in 1995. 2) In the proportion of male to female, showed a ratio of three to one [male;72%(18), female;28%(7)] and showed third decade(7 cases), fifth decade(6 cases), sixth decade(4 cases) in comparison of age. 3) The geographical distribution of occurrence showed island areas(64%), inland areas(36%), and 6 cases of inland areas occurred of mountainous region. 4) One cause of events was traumatic causes(52%), which included traffic accident(5 cases) & fall down(4 cases). The other cause was disease(48%). 5) The expended time of helicopter from start to arrive at accident spot within Chon-nam area is from ten minutes to forty-five minutes, as mean expended time is thirty minutes. 6) The expended time from occurrence of patient to admitted hospital was from minimum twenty-five minutes to maximum eight hours and forty minutes, as expended time is one hundred and eighty-six minutes. 7) After arrived at landing zone, via emergency department to admitted patient; 12 cases, performed emergency operation; 4 cases, D.O.A. state; 2 cases, hopeless discharge; 2 cases, transfer to other hospital; 4 cases. Up to now, the present condition of helicopter-transport system was looked upon as petty affairs, and determination of transport was swayed by geographic element rather than severity of injury. And so, it is considered that epochal turning point is necessary for development of helicopter-transport system.

Escherichia coli 패혈증 환자에 합병된 대칭적 하지 말단 괴사증 1예

남해성,유진홍,권순석,민준기,조현선,박민경,심병주,남유정,이지인,김진수,길욱현,조근종,신완식 대한감염학회 2005 감염과 화학요법 Vol.37 No.6

We have encountered a rare case of symmetrical peripheral gangrene complicating Escherichia coli sepsis in a 47-years-old male. He was successfully treated with antibiotics, anticoagulants, and vasodilator. To our knowledge, this is the first report on symmetrical peripheral gangrene complicating E. coli sepsis in Korea.

랫드에서 법제유황과 비법제유황의 독성비교

김근수, 정유리, 이남진, 홍성희, 유종훈, 정재황, 강종구 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.2

본문참고

비산회재로부터 합성된 제올라이트를 이용한 Polypropylene 촉매열분해에 관한 연구

남윤미,이진홍,김성수,정수현 한국공업화학회 2003 응용화학 Vol.7 No.1

Zeolites were synthesized from fly ash and used as catalysts for pyrolysis of polypropylene(PP) Zeolite synthesis was carried out by fusion methods employing three different NaOH/Fly ash rations in weight The catalytic performance of the fly ash-derived zeolite pyrolysis products. The properties of the fly ash-derived zeolites were characterized by XRD, SEM, BET, NH_3-TPD. It was found that the optimum NaOH/fly ash weight ratio was 1.2 in terms of the simulated boiling point distribution. The catalytic perfomance of the fly ash-derived zeolite was significantly improved by ion exchanging of zeolite from Na from(FAZ) to H from(HFAZ) The boiling point distributions of liquid pyrolysis products obtained over HFAZ were significantly lower than that obtained zeolite.

LED 전광판 제어 ASIC의 설계

남상길,조경연,이수범,김종진 釜慶大學校 1997 釜慶大學校 論文集 Vol.2 No.2

The wide spread of multimedia system demands a wide viewing angle display device which can imform a message to many peoples in open area. This paper is about the design and simulating of a large viewing LED pannel control ASIC(Application Specific Integrated Circuit). This LED pannel control ASIC runs on 16bit micropricessor MC68EC000 and has following functions : 16 line interlaced LED pannel contriller, memory controller, 16 channel priority interrupt cotroller, 2 channel direct memory access controller, 2 channel 12 bit clcok and timer, 2channel infrared remocon receiver, 2 channel RS-23C with 16 byte FIFO, IBMPC/AT compatible keyboard interface, battery battery backuped real time clock and timer,2controller, battery backuped 256 byte SRAM and watch dog timer. The 0.6 micronCMOS Sea of Gate is used to desing the ASIC in amiunt of about 39,000 gates,and it is simulated at 48MHz

성견에서 교정적 고정원으로서의 티타늄 미니스크류에 대한 연구 : An experimental investigation in dogs

윤병수,최병호,이원유,김경남,심형보,박진형 대한치과교정학회 2001 대한치과교정학회지 Vol.31 No.5

교정치료에서 원하는 치아이동을 위해서는 안정된 고정원이 필요한데 티타늄 미니스크류가 매식과 제거가 쉽고, 구강 내 여러 부위에서 적용이 가능하고, 환자가 느끼는 불편감이나 비용적인 부담이 적고, 제거 후에 치유가 빠르게 진행될 수 있는 등의 장점이 있어 최근에 교정적 고정원으로 사용되기 시작하였다. 티타늄 미니스크류를 교정적 고정원으로 사용한 임상 예들이 여러 편 발표되었는데 미니스크류의 이완이 가장 큰 실패의 원인으로 보고되고 있다. 그러나 지금까지 보고된 논문들에서 교정적 고정원으로 안정성을 줄 수 있는 스크류의 식립 길이에 관한 연구가 없는 상태이다. 교정적 고정원으로 미니스크류를 효과적으로 사용하기 위해서는 식립 부위에 따른 골구조와 골밀도 차이를 고려한 식립 길이에 관한 기준이 필요하다. 이에 본 연구에서는 성견의 상악골과 하악골에서 직경 2mm 티타늄 미니스크류를 다양한 길이로 식립하고 교정력을 적용한 후 그 안정성을 평가하여 교정적 고정원으로 사용될 수 있는 미니스크류의 식립 길이를 결정하고자 하였다. 미니스크류가 상악에서는 6mm이상, 하악에서는 4mm 이상이 골 내에 식립될 때 8주 동안 200g의 교정력에 동요도나 위치변화를 보이지 않았다. 식립 부위로는 부착치은 부위 치근 사이에 식립될 때 구강청결이 유지되고 미니스크류 주변 치은조직에 자극을 주지 않아 정상적인 조직으로 유지 될 수 있었다. 또한 교정력 적용 8주 후 치근단 방사선 사진검사에서 스크류 주변 치근 흡수나 치조골 흡수, 치주 인대 손상이 관찰되지 않았다. 따라서 상 ㆍ 하악 골밀도와 골구조의 차이를 고려하여 미니스크류의 골내 식립 길이를 적절히 조절함으로써 교정적 고정원으로 티타늄 미니스크류가 효과적으로 사용될 수 있다고 생각된다. Titanium miniscrews are being used increasingly as an anchorage for tooth movement, because they are easy to place and to remove, increase the number of sites available, give minimum strain to patients regarding surgical procedures, and offer uneventful healing after removal. The use of titanium miniscrews as an orthodontic anchorage has been reported in clinical case reports, but clinicians hove experienced screw loosening when using such screws. To our knowledge, there are no published reports evaluating the stability of miniscrews. Information about the length of miniscrews used in relation to the location is of some importance, as stability will vary depending on bone quality. The purpose of this study was to evaluate a variety of lengths of miniscrews (diameter: 2mm) which were inserted in maxilla or mandible and to demonstrate in a dog moedl which miniscrew provides fundamental stability in the jaws. 10 mm long miniscrews in the maxilla and 8mm long miniscrews in the mandible showed no clinical mobility and retained their position throughout an 8 weeks force (200g) application. The mucosal condition around the screws was healthy in cases in which miniscrews were inserted in the alveolar bone between the roots and the head of the screws emerged into the attached gingiva. When the force application was terminated, radiographic analysis revealed neither root resorption nor periodontal pathology around the miniscrews that remained stable during the entire treatment period. This study suggests that if titanium miniscrews with adequate length are properly used depending on the location, they provide sufficient stability for orthodontic anchorage.

비강에 발생한 반전성 유두종 1예

도남용,김왕수,박성웅,신태호,전호종,박영진 대한이비인후과학회 1981 대한이비인후과학회지 두경부외과학 Vol.24 No.4

Rowe Cell 을 利用한 粘土試料 샘플링의 攪亂程道 推定

최명진,김민욱,전남수,김필규 忠南大學校 産業技術硏究所 1999 산업기술연구논문집 Vol.14 No.1

Recently many studies which related to effective sampling method have been progressed and valuable data accumulated in foreign country. But, domestic technique is insufficient to evaluate the degree of disturbance so it is difficult to seek soli parameter. Thus the grasp of the degree of disturbance is effential to increase accuracy of reasonable parametern from laboratory tests. Also, the degree of disturbance of it was determined. Oedometer tests and Rowe cell consolidation rests reappeared in -situ state were conducted. And its result was compared and analyzed to deduces the degree of disturbance. From these tests, It can be inferred that the compression index generally is not affected and swelling index(C )and preconsolidation load (P )are increased because of the disturbance of soil. Also, it can be considered that consolidation curves given by two tests is little affected from desturbance. Conclusively, the results of two tests indicated that void ratio(e) and swilling index is independent of the degree of disturbance and it is reasonable to evaluate the degree of disturbance through it.

경부 관통상으로 인한 사지 마비 1례

박 진,이경운,김춘호,김성중,조남수,안태훈 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.3

A case of quadriplegia resulted from cervical penetrating wound was admitted to chosun university hospital intensive care unit, and undergone steroid therapy and antibiotics therapy. On the 6th hospital day, the septic complication was developed and it proved to be bacterial meningitis by CSF examination. Broad spectrum antibiotics was administered immediately after and the diagnosis and the patient was monitored carefully. The bacterial meningitis was improved and no other serious complication was developed. The patient was discharged with permanent neurologic deficit, quadriplegia.