Physicochemical Characteristics of Wine Made from Domestic Grapes

Mi-Ran Yang,Eun-Jung Bae,Chan-Woo Kim,BoRa Lim,Youn-Young Her,Young-Sik Park,Seok-Tae Jeong 한국식품영양과학회 2020 한국식품영양과학회 학술대회발표집 Vol.2020 No.10

The Essential Oil of Artemisia iwayomogi Kitamura Induces Apoptosis on Human Oral Epidermoid Carcinoma Cells

Mi-Ran Jeong,Jeong-Dan Cha,Kyung-Yeol Lee,Bong-Seop Kil,Jong-Hyun Han,Young-Eun Lee 한국식품과학회 2007 Food Science and Biotechnology Vol.16 No.4

The aerial part of Artemisia iwayomogi Kitamura has traditionally been used for inflammation, infectious disease, cancer, pyretic, diuretic, liver protective effect, and choleretic purposes in Korea. We investigated that the essential oil induces apoptosis in KB cell as evidenced by Hoechst-33258 dye staining, flow cytometry (cell cycles), and DNA fragmentation for nuclear condensation and Western blotting for activation of caspases-3, -8, -9, Bax, Bcl-2, cytochrome c, and poly (ADPribose) polymerase (PARP) cleavage. In the present study, we found that the essential oil could induce apoptosis in KB cells, as characterized by DNA fragmentation, activation of caspase-3, -8, and -9, and PARP cleavage. The efficacious induction of apoptosis was observed as a dose-dependent. The essential oil-induced apoptotic cell death was accompanied by up-regulation of Bax and down-regulation of Bcl-2. The essential oil also caused the loss of mitochondrial membrane potential and cytochrome c release from mitochondria to cytosol. These findings indicate that mitochondrial pathways might be involved in the essential oil-induced apoptosis and enhance our understanding of the anticancer function of the essential oil in herbal medicine.

Induction of Apoptosis in Human Oral Epidermoid Carcinoma Cells by Sophoraflavanone G from Sophora flavescens

Jeong-Dan Cha,Mi-Ran Jeong,Young-Eun Lee,Kyung-Yeol Lee 한국식품과학회 2007 Food Science and Biotechnology Vol.16 No.4

Sophora flavescens AITON (Leguminosae) is a typical traditional Korean medical herb considered to exhibit antibacterial, anti-inflammatory, and antipyretic effects, and is also used for the treatment of skin and mucosal ulcers, sores, diarrhea, gastrointestinal hemorrhage, arrhythmia, and eczema. In this study, the compound sophoraflavanone G was isolated from the dried roots of S. flavescens by bioassay-guided fractionation. We then investigated the effects of various concentrations of sophoraflavanone G on cell viability and the induction of apoptosis in KB cells after an incubation of 24 hr. The results were determined by the following methods: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-terazolium bromide (MTT) assay, Hoechst-33258 dye staining, flow cytometry (cell cycle), and Western blotting for caspase-3 and poly (ADP-ribose) polymerase (PARP). We found sophoraflavanone G induced the apoptosis of KB cells in a dose-dependent manner that was verified by DNA fragmentation, apoptotic bodies, the sub-G1 ratio, caspase-3 activity, and cleavage of PARP. These results suggest that sophoraflavanone G has potent anti-proliferative effects on human oral epidermoid carcinoma cells, with the induction of apoptosis.

Induction of Apoptosis in Human Oral Epidermoid Carcinoma Cells by Essential Oil of Chrysanthemum boreale Makino

Jeong-Dan Cha,Mi-Ran Jeong,Young-Eun Lee 한국식품과학회 2005 Food Science and Biotechnology Vol.14 No.3

Tyrosinase Inhibitory Activity of Rosmarinic Acid on Human Skin Melanoma Cells Treated with Hydrogen Peroxide

Jeong-Ran Kang,Sun-Mi Yoo 한국실험동물학회 2009 Laboratory Animal Research Vol.25 No.2

The purpose of this study was to evaluate the tyrosinase inhibitory activity of rosmarinic acid on the cultured human skin melanoma cells damaged by reactive oxygen species (ROS). The cytotoxicity effect was analyzed by MTT assay for cell viability, tyrosinase inhibitory activity after treating the cells with or without hydrogen peroxide (H₂O₂) for 1-10 hours. Light microscopic study was also carried out for morphological changes of the cells and tyrosinase activities. H₂O₂ decreased the cell viability in the cultured human melanoma cells in a dose- and time-dependent manner after cells had been treated with 20-80 ?M of H₂O₂ for 7 hours. IC?? value of H₂O₂ by the MTT assay was 80 ?M. Rosmarinic acid increases cell viability, inhibitory activity of tyrosinase. In light microscopic study, the number of human skin melanoma cells treated with H₂O₂ was decreased compared with control, and H2O2 activated tyrosinase activity, which was observed through darkened cell color (mid-activity, +++). However, colors of cells treated with Rosmarinic acid changed from white to brown in cell staining due to tyrosinase activity (moderate-activity, ++). The treatment of the cells with H₂O₂ shows highly toxic effects on cultured human skin melanoma cells. Rosmarinic acid increases cell viability and tyrosinase inhibitory activity in the cells treated with H₂O₂. It is considered that Rosmarinic acid shows tyrosinase inhibitory effect on ROS such as H₂O₂.

Mitigation of Rayleigh crosstalk using noise suppression technique in 10-Gb/s REAM-SOA.

Jeong, Jong Sool,Kim, Hyun-Soo,Choi, Byung-Seok,Kim, Dong Churl,Kim, Ki-Soo,Park, Mi-Ran,Kwon, O-Kyun Optical Society of America 2012 Optics express Vol.20 No.24

<P>We demonstrate a mitigation of Rayleigh back-scattering (RBS) impact in 10-Gb/s reflective electroabsorption modulator monolithically integrated with semiconductor optical amplifier (REAM-SOA). The technique is based on the intensity-noise suppression of the centralized incoherent seed-light, which enables smooth evolution of deployed DWDM applications. We exhibit the power penalty of less than 1 dB at the large RBS crosstalk value of about 8 dB when the optical power of seed-light is lowered about -10 dBm.</P>

Antimicrobial and Anti-inflammation Activities of Extracts Pinus koraiensis Leaf extracts

Mi Kyoung Lee,Hye Ran Kim,Jeong Hwan Ko,Hyo-Jung Lee,Min Ho Lee 한국식품영양과학회 2015 한국식품영양과학회 학술대회발표집 Vol.2015 No.8

Treatment Modality Based Survival in Gastric Carcinoma Patients with Stand-Alone Peritoneal Metastasis: a Case-Control Study

Jeong, Oh,Jung, Mi Ran,Kang, Ji Hoon The Korean Gastric Cancer Association 2021 Journal of gastric cancer Vol.21 No.2

Purpose: To date, there are no promising treatments for gastric carcinoma with peritoneal metastasis. Some researchers have suggested a survival benefit of gastrectomy in select patients. This study investigated the survival of gastric carcinoma patients with stand-alone peritoneal metastasis according to the type of treatment modality. Materials and Methods: We reviewed the data of 132 patients with gastric carcinoma and stand-alone peritoneal metastasis. We performed gastrectomy when the primary tumor was deemed resectable and systemic chemotherapy was administered. We analyzed patient survival according to the type of treatment, and the prognostic value of gastrectomy was evaluated in univariate and multivariate models. Results: Among all patients, 70 underwent gastrectomy plus chemotherapy, 20 underwent gastrectomy alone, 36 underwent chemotherapy alone, and 6 received supportive care. The median patient survival was 13 months. Patients who underwent gastrectomy had significantly longer survival than those who did not undergo gastrectomy (14 vs. 8 months, P<0.001). Patients who received chemotherapy showed significantly longer survival than those who did not (13 vs. 7 months, P=0.032). Patients who underwent gastrectomy plus chemotherapy showed better survival than those who underwent other treatments. In multivariate analysis, gastrectomy was found to be an independent prognostic factor (hazard ratio, 0.52; 95% confidence interval, 0.33-0.82) in addition to chemotherapy. Conclusions: Our study showed that patients who underwent gastrectomy plus chemotherapy had the best survival. Although the survival benefit of gastrectomy remains uncertain, it is a favorable prognostic indicator in patients with stand-alone peritoneal metastasis.

Comparison of Biological Activities on Extracts and Fractions in Compositae Plants

Jeong-Ran Kang,Eun-Mi Yu,Kap-Hoon Han 중소기업융합학회 2019 융합정보논문지 Vol.9 No.9

본 연구는 우리나라 전역에 자생하는 국화류3종 학슬, 희렴, 한련초를 대상으로 MeOH 80% 추출물 및 헥산, 클로 로포름, 에틸아세테이트, 부탄올 및 물분획을 이용한 항균활성, 항산화 및 tyrosinase 저해활성효과에 대한 연구를 통해 기능성 화장품소재로서의 이용가능성을 확인하고자 하였다. 연구결과 항균활성의 경우 MeOH 80% 추출물에서는 한련초 와 용매분획 중에서는 학슬의 헥산분획, 희렴의 클로로포름분획, 에틸아세테이트 분획, 한련초의 클로로포름분획을 중심 으로 항균력을 나타내었다. 항산화 활성의 경우 3종 모두 용매분획 중 에틸아세테이트, 부탄올 분획에서 90%이상의 효과 를 나타내었다. Tyrosinase 저해활성의 경우 희렴과 학슬의 에틸아세테이트 분획물에서 우수한 활성을 나타내었다. 이상 의 국화류3종 MeOH 80%추출물 및 분획물에서 항산화 및 미백작용이 우수하므로 기능성화장품소재 개발 활용이 가능할 것으로 생각된다. This study was conducted to investigate the anti-oxidative, anti-oxidative and tyrosinase inhibitory effects of MeOH 80% extract and hexane, chloroform, ethyl acetate, butanol and aqueous fraction on three kinds of compositae plants in Korea. In the antimicrobial effect, the extract and chloroform fraction of Eclipta prostrata and hexane fraction of Carpesium abrotanoides L. and chloroform fraction of Siegesbeckia glabrescens exhibited significant inhibition. The antioxidant activity of ethyl acetate and butanol fractions was more than 90% in all three plants. In case of tyrosinase activity, showed a potent inhibition ethlyacetate fraction of Siegesbeckia glabrescens and Carpesium abrotanoides L, which were higher than control group. In MeOH 80% extracts, there was not found to have antimicrobial, anti-oxidant and tyrosinase inhibitory activity, however there was ethylacetate fraction of Siegesbeckia glabrescens to show effectss commonly in the three assay system.

TAZ as a novel enhancer of MyoD‐mediated myogenic differentiation

Jeong, Hana,Bae, Sujung,An, Su Yeon,Byun, Mi Ran,Hwang, Jun‐,Ha,Yaffe, Michael B.,Hong, Jeong‐,Ho,Hwang, Eun Sook Federation of American Society for Experimental Bi 2010 The FASEB Journal Vol.24 No.9

<P>Myoblast differentiation is indispensable for skeletal muscle formation and is governed by the precisely coordinated regulation of a series of transcription factors, including MyoD and myogenin, and transcriptional coregulators. TAZ (transcriptional coactivator with PDZ-binding motif) has been characterized as a modulator of mesenchymal stem cell differentiation into osteoblasts and adipocytes through its regulation of lineage-specific master transcription factors. In this study, we investigated whether TAZ affects myoblast differentiation, which is one of the differentiated lineages of mesenchymal stem cells. Ectopic overexpression of TAZ in myoblasts increases myogenic gene expression in a MyoD-dependent manner and hastens myofiber formation, whereas TAZ knockdown delays myogenic differentiation. In addition, enforced coexpression of TAZ and MyoD in fibroblasts accelerates MyoD-induced myogenic differentiation. TAZ physically interacts with MyoD through the WW domain and activates MyoD-dependent gene transcription. TAZ additionally enhances the interaction of MyoD with the myogenin gene promoter. These results strongly suggest that TAZ functions as a novel transcriptional modulator of myogenic differentiation by promoting MyoD-mediated myogenic gene expression.</P>