http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression
Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11
<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>
Mi-Na Han,Jeong-Ho Kim,Sang-Kab Lee,Kyong-Ok No,Mun-Hui Chae,Woo-Kyoung Cho,Jong-Hwa Lee,Rae-Hoon Jang,Chang-Seop Kim,Hyeon-Seop Byeon 한국동물위생학회 2020 韓國家畜衛生學會誌 Vol.43 No.2
This case describes outbreaks of acute aspergillosis in a red-crowned crane. A six-month-old, male, crane had showed clinical signs (i.e. anorexia, performance loss, ruffled feathers and drooped wings and open mouth breathing, etc.) before death. In necropsy examination, spherical to oval nodules dissemi-nated from the respiratory tract to other organs. Those nodules were formed predominantly in air sacs, lung, peritoneum, serosa of esophagus and trachea. The nodules varied in size from 1 mm to over 1cm and the color was white to yellow. Microscopically, most of lung architecture were replaced by multiple foci which were characterized by well demarcated eosinophilic and karyorrhetic debris and surrounded by numerous Inflammatory cell. Most within necrotic center of the nodules, large numbers of fungal hyphae were present. Microbiology result indicated fungal growths on sabroud dextrose agar and bacte-rial growths on blood agar. Bacteria identified as E. rhusiopathiae using MALDI-TOF (microflex, BRUKER, USA) and fungi identified as A. fumigatus, A. terreus by sequencing the ITS1 and ITS4 regions. To con-firm the route of infection, we checked the existence of the same pathogens in cohabitant (i.e. mother crane). The young age and weakened immunity (i.e. bacterial infection, etc.) causes fatal aspergillosis in birds.
( Mi Ok Woo ),( Jin Hyoung Lee ),( Seong Hyu Shin ),( Kong Sik Shin ),( Hee Jong Koh ),( Seok Cheol Suh ) 한국육종학회 2015 Plant Breeding and Biotechnology Vol.3 No.1
Most Bt transgenic rice events have an underlying grain cost depending on the insect pressure in the field. This study was conducted (1) to determine if the cry1Ac/bar genetically modified (GM) rice (designated Agb0101) based on a Korean japonica rice cultivar has any underlying grain cost and (2) to develop cry1Ac/bar GM rice lines with yield performance similar to those of Korean elite cultivars via crossbreeding. A three-year field experiment showed that Agb0101 had a high underlying grain cost equivalent to 18% grain yield loss, and this cost was mainly due to the grain number per panicle. Moreover, it was found that the cry1Ac/bar transgene was inherited as a single dominant gene. BC1F4-F5 crossbred progeny derived from an anther culture of Agb0101-showed phenotypic resemblance to non-transgenic Korean elite cultivars, resulting in a decrease in the grain yield cost of Agb0101 from 27% to 10%. These results suggest that Bt-GM rice-oriented cross-fertilization with a non-GM elite cultivar followed by two- or threefold selection is a feasible method to minimize or remove the underlying grain cost of the Bt transgenic insect-resistant rice line.
Improve Storability of the Fresh-cut Vegetables with Propolis
Woo Soon-ok,Sang-mi Han,In-pyo Hong,Joo-hong Yeo 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10
Propolis is a health food, known that high antioxidant and antimicrobial effects, fresh cut vegetables that rapidly increasing consumption has recently faced the problem storability fall down after washing. To improve storability of fresh cut vegetables are being carried out various studies. In this study, using the characteristics of propolis we were performed to improve the storability of fresh cut vegetables. There was prepared in 20% solution of propolis extracts, by using this solution, propolis solution prepared diluting 0.001 to 1%, were dipped in fresh vegetables (cabbage lettuce, sesame leaf, and lettuce). Vegetables were measured the sensory evaluation and hardness after each treatment by placing in a certain period of time at room temperature and refrigerator. When cabbage lettuce was stored for 3 days at refrigerater, sensory evaluation that the cleavage site is not generated browning in 0.1% propolis solution showed the best results, the hardness was the most excellent. During refrigerated storage after treatment in a 0.01% solution of propolis sesame leaves showed good sensory evaluation include freshness, morphological change of leaves, and high hardness. After treatment propolis of 1% solution at room temperature, Sesame leaves showed the most heavily defensive fragility phenomenon after three days. It was found that the appearance of the side effect, when the concentration of the propolis is rather high. If lettuce is chilled to handle after propolis treatment, after 10 days in the solution from 0.01 to 0.1% showed excellent sensory evaluation, and high hardness. These results confirmed that the storage stability is excellent compared to non-treated as if diluted to 0.1-0.01% propolis solution is to improve the shelf life of fresh vegetables using.
Characterization of 4 different alleles in GE locus affectig the embryo size rice
Mi-Ok Woo,Hanso Ju,Hee-Jong Koh 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10
The giant embryo(ge) mutation was genetically mapped to chromosome 7 by Koh et al. The ge mutations were analyzed at the morphologic and genetic level by Hong et al. And this publication linked the GE gene as being required for proper endosperm development. Researchers in Dupont cloned the GE gene, sequenced and characterized the ge alleles of the mutants. the three giant embryo mutant lines we obtained by treating MNU to Hwacheongbyeo, a japonica type Korean rice cultivar, differed in their embryo and endosperm size and their embryo/endosperm ratios. We named these alleles affecting the embryo size in rice GE, ge-m, ge, ge-s (GE: the wild type, ge-m: embryo slightly larger than in the wild type, ge: large embryo, ge-s: embryo even larger than in ge). The ORFs and introns of the four different alleles in the GE locus were sequenced and compared with the corresponding sequences in the patent that dupont presented. Each mutant alleles sequence showed a few SNPs in the ORF region. Two SNPs were shared among the three mutant lines and each mutant line hab one or two additional SNPs. Further studies are in progress regarding the GE RNA expression level in each mutant line and the F1 seed phenotypes and allelism relationship among the mutant lines.
Mi-Ok Woo,Hyung-Ho Park,Jung-Hyun Nam,Nam-Chon Paek 韓國作物學會 2001 Korean journal of crop science Vol.46 No.1
Barley Yellow Dwarf Virus (BYDV) has been a major disease causing a severe loss of yield in winter cereals worldwide. It has been recently reported that BYDV occurs frequently in wheat field and also causes serious yield reduction in Korea. This study was performed to investigate the regional distributions of BYDV strains in Korea and to identify the resistant cultivars or lines of wheat to the predominant BYDV strains, providing basic information for the breeding of BYDV-resistant wheat varieties. Using RT-PCR and EcoRI digestion methods, the regional distribution of BYDV strains in Korea from 1999 to 2000 showed that PAV strain was mainly detected about 65% (Vic-PAV 52.6% ; CN-PAV 47.4%) and MAV strain about 3%. Using ELISA test for the examination of BYDV resistance with 17 cultivars and 4 lines among Korean wheat, three cultivars, Gurumil, Topdongmil, and Olgurumil, were susceptible to BYDV and the others were resistant. In plant growth and yield component responses to BYDV infection, Gurumil showed significant difference between the uninfected and the infected, suggesting the most susceptible to BYDV among Korean wheat, but Eun-pamil and Seohae118 did no difference, an indication that they have the highest resistance.
Woo-Jin Jeon,Eun-Kyoung Lee,Young-Jeong Lee,Ok-Mi Jeong,Yong-Joo Kim,Jun-Hun Kwon,Kang-Seuk Choi 대한수의학회 2008 Journal of Veterinary Science Vol.9 No.3
Despite the intensive vaccination policy that has been put in place to control Newcastle disease virus (NDV), the recent emergence of NDV genotype VII strains in Korea has led to significant economic losses in the poultry industry. We assessed the ability of inactivated, oil-emulsion vaccines derived from La Sota or Ulster 2C NDV strains to protect chickens from challenge with Kr-005/00, which is a recently isolated Korean epizootic genotype VII strain. Six-week-old SPF chickens were vaccinated once and challenged three weeks later via the eye drop/intranasal route. All vaccinated birds were fully protected from disease, regardless of the vaccine strains used. All vaccinated and challenged groups showed significant sero-conversion 14 days after challenge. However, some vaccinated birds, despite being protected from disease, shed the challenge virus from their oro-pharynx and cloaca, albeit at significantly lower titers than the unvaccinated challenged control birds. The virological, serological, and epidemiological significance of our observations with regard to NDV disease eradication is discussed.