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Candidate Genes with Ovulation by Differential Display PCR in Small Tail Han Sheep
Liu, Shufang,Li, Hongbin,Song, Xuemei,Wang, Aihua,Wei, Caihong,Du, Lixin Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.9
To find the candidate genes concerned with ovulation rate of sheep, Differential Display Reverse Transcription Polymerase Chain Reaction was employed to find the differently expressed cDNA controlling ovulation in the Small Tail Han sheep of polyembryony and in Tan sheep of single birth. Twenty-four primer pairs of three anchored primers and eight arbitrary primers were assembled to amplify the specialized bands from these sheep. Positive cross tests were applied to optimize the ascertainable PCR conditions in which different special bands can be identified by silver strain in one PCR tube. After eliminating the false positive PCR products by Northern hybridization, 24 differential display bands were acquired from the ovary in the Small Tail Han sheep. These EST bands were sequenced and 18 different ESTs were found in which five ESTs had several copies and 13 ESTs had only one copy. Comparing these ESTs with homologous sequences by BLAST in the GenBank, there were six ESTs with known open reading frame (ORF) and function, three ESTs with known ORF and no function, and 9 ESTs without homologous sequence. These ESTs partly represent several genes such as NOS2, tensin, TCRA, CDKN1A, ESR1 and ACTB which express especially in Small Tail Han sheep.
( Aihua Zhang ),( Shouyi Yang ),( Jianjun Li ),( Chunlei Li ),( Zhoufeng Liu ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.8
We investigate the channel state information (CSI) in multi-input multi-output (MIMO) cooperative networks that employ the amplify-and-forward transmission scheme. Least squares and expectation conditional maximization have been proposed in the system. However, neither of these two approaches takes advantage of channel sparsity, and they cause estimation performance loss. Unlike linear channel estimation methods, several compressed channel estimation methods are proposed in this study to exploit the sparsity of the MIMO cooperative channels based on the theory of compressed sensing. First, the channel estimation problem is formulated as a compressed sensing problem by using sparse decomposition theory. Second, the lower bound is derived for the estimation, and the MIMO relay channel is reconstructed via compressive sampling matching pursuit algorithms. Finally, based on this model, we propose a novel algorithm so called sparsity adaptive expectation maximization (SAEM) by using Kalman filter and expectation maximization algorithm so that it can exploit channel sparsity alternatively and also track the true support set of time-varying channel. Kalman filter is used to provide soft information of transmitted signals to the EM-based algorithm. Various numerical simulation results indicate that the proposed sparse channel estimation technique outperforms the previous estimation schemes.
The Association of SERPINE2 Gene with COPD in a Chinese Han Population
Aihua Wang,Wei Xiao,Yingqiu Yin,Ping Chen,Qiji Liu,Qinfeng Yu 연세대학교의과대학 2011 Yonsei medical journal Vol.52 No.6
Purpose: Polymorphisms of several candidate genes have been studied and associated with the development of chronic obstructive pulmonary disease (COPD). One such candidate is the SERPINE2 (Serpin peptidase inhibitor, clade E member 2) gene. Materials and Methods: To assess whether the SERPINE2 gene is associated with COPD in a Chinese Han population. Samples were collected from a Chinese Han population and analyzed for the association of single nucleotide polymor phisms (SNPs) or haplotypes of SERPINE2 gene with COPD in a case-control study. Three SNPs including rs840088 G/A in intron 1, rs1438831 A/G in 5’ upstream sequence and rs3795879 G/A in intron 3 were detected using the polymerase chain reaction (PCR)-based restriction fragment length polymorphism technique in 409 COPD subjects and 411 controls. Genotyping of the SREPINE2 polymorphisms at positions rs840088, rs1438831and rs3795879 was performed. Results: We found that none of the rs840088G/A, rs1438831G/A and rs3795879 G/A polymorphisms were associated with the disease. The p-values were 0.630, 0.208 and 0.398 respectively. Conclusion: Our data suggested that there was no significant association between SERPINE2 polymorphism and COPD susceptibility in the Chinese Han population.
Liu, Shuangli,Xu, Yonghua,Gao, Yugang,Zhao, Yan,Zhang, Aihua,Zang, Liansheng,Wu, Chunsheng,Zhang, Lianxue The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.1
Background: The lepidopteran Asiatic corn borer (ACB), Ostrinia furnacalis (Guenee), has caused huge economic losses throughout the Asian-Western Pacific region. Usually, chemical pesticides are used for the control, but excessive use of pesticides has caused great harm. Therefore, the inartificial ecotypic pesticides to ACB are extremely essential. In our previous study, we found that panaxadiol saponins (PDS) can effectively reduce the harm of ACB by causing antifeedant activity. Therefore, it is necessary to reveal the biological molecular changes in ACB and the functionary mechanism of PDS. Methods: We analyzed the global transcription of ACB with different PDS concentration treatment (5 mg/mL, 10 mg/mL, and 25 mg/mL) by high-throughput sequencing and de novo transcriptome assembly method. Results: PDS treatment could cause the changes of many gene expressions which regulate its signal pathways. The genes in peroxisome proliferator-activated receptor (PPAR) signaling pathway were significantly downregulated, and then, the downstream fatty acid degradation pathway had also been greatly affected. Conclusion: Through this experiment, we hypothesized that the occurrence of antifeedant action of ACB is because the PDS brought about the downregulation of FATP and FABP, the key regulators in the PPAR, and the downregulation of FATP and FABP exerts further effects on the expression of SCD-1, ACBP, LPL, SCP-X, and ACO, which leads to the disorder of PPAR signaling pathway and the fatty acid degradation pathway. Not only that, PDS treatment leads to enzyme activity decrease by inhibiting the expression of genes associated with catalytic activity, such as cytochrome P450 and other similar genes.
A Stator-Separated Axial Flux-Switching Hybrid Excitation Synchronous Machine
Liu, Xiping,Zheng, Aihua,Wang, Chen Journal of International Conference on Electrical 2012 Journal of international Conference on Electrical Vol.1 No.4
In this paper, a stator-separated axial flux-switching hybrid excitation synchronous machine (SSAFHESM) is presented, of which the structure and operational principle are introduced. The magnetic field distribution under different excited currents is analyzed, and some characteristics including flux-linkage, EMF and field control ability are studied by finite element analysis (FEA). Tests are carried out on a 12/10-pole prototype machine to validate the analysis results, and an excellent agreement is obtained.
Liu, Xiping,Wang, Chen,Zheng, Aihua Journal of International Conference on Electrical 2012 Journal of international Conference on Electrical Vol.1 No.3
The operation principle of an axial flux-switching hybrid excitation synchronous machine (AFHESM) is analyzed and its topology structures are proposed in this paper. After some comprehensive analysis of the operation principle to axial flux electrical machine, flux-switching electrical machine and hybrid excitation electrical machine, the operation principle of AFHESM is given. Combined with some typical topological structures of hybrid excitation electrical machine, some possible topological structures are proposed and some comprehensive comparisons are carried out. The analysis results show that the stator-separated AFHESM has some advantages such as less AM turns, less impact on the demagnetization of PM, less magnetic flux-leakage and higher efficiency compared to other topologies.
Shuangli Liu,Yonghua Xu,Yugang Gao,Yan Zhao,Aihua Zhang,Liansheng Zang,Chunsheng Wu,Lianxue Zhang 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.1
Background: The lepidopteran Asiatic corn borer (ACB), Ostrinia furnacalis (Guenee), has caused hugeeconomic losses throughout the Asian-Western Pacific region. Usually, chemical pesticides are used forthe control, but excessive use of pesticides has caused great harm. Therefore, the inartificial ecotypicpesticides to ACB are extremely essential. In our previous study, we found that panaxadiol saponins(PDS) can effectively reduce the harm of ACB by causing antifeedant activity. Therefore, it is necessary toreveal the biological molecular changes in ACB and the functionary mechanism of PDS. Methods: We analyzed the global transcription of ACB with different PDS concentration treatment (5 mg/mL, 10 mg/mL, and 25 mg/mL) by high-throughput sequencing and de novo transcriptome assemblymethod. Results: PDS treatment could cause the changes of many gene expressions which regulate its signalpathways. The genes in peroxisome proliferatoreactivated receptor (PPAR) signaling pathway weresignificantly downregulated, and then, the downstream fatty acid degradation pathway had also beengreatly affected. Conclusion: Through this experiment, we hypothesized that the occurrence of antifeedant action of ACBis because the PDS brought about the downregulation of FATP and FABP, the key regulators in the PPAR,and the downregulation of FATP and FABP exerts further effects on the expression of SCD-1, ACBP, LPL,SCP-X, and ACO, which leads to the disorder of PPAR signaling pathway and the fatty acid degradationpathway. Not only that, PDS treatment leads to enzyme activity decrease by inhibiting the expression ofgenes associated with catalytic activity, such as cytochrome P450 and other similar genes.
Suppression and stability analysis of frequency coupling effect in grid‑connected inverters
Xiaohuan Wang,Aihua Yang,Yang Liu,Xiaojun Zhao 전력전자학회 2023 JOURNAL OF POWER ELECTRONICS Vol.23 No.6
With the proposal of the “dual carbon target” in China, the rapid development of renewable energy, mainly photovoltaic and wind power, has been promoted. However, a large amount of renewable energy connected to a grid results in its proportion in the power system going from a low state to a high state. Under a high proportion, the asymmetry of the control structure or parameters in the three-phase grid-connected inverter controller lead to a strong coupling relationship between the sub/super synchronous frequency components, or frequency coupling effect (FCE). This phenomenon can deteriorate the power quality of the inverter system, amplify the harm of frequency oscillation, and even cause system disassembly. To solve the above problems, a unified impedance model considering the FCE induced by the phase-locked loop (PLL), the current loop (CL), and the power outer loop (POL) is established. Based on the established output impedance model, a parameter optimization theoretical analysis method is designed considering the critical stability of the PLL bandwidth and the CL asymmetry degree. Meanwhile, an improved control strategy for the PLL and the POL is proposed. Experimental results show that the proposed parameter optimization and structure improvement strategies can effectively suppress the infl uence of frequency coupling and enhance system stability. Finally, simulation and experimental results verify the correctness of the theoretical analysis and the eff ectiveness of the proposed strategy.
Energy spread minimization in a cascaded laser wakefield accelerator via velocity bunching
Zhang, Zhijun,Li, Wentao,Liu, Jiansheng,Wang, Wentao,Yu, Changhai,Tian, Ye,Nakajima, Kazuhisa,Deng, Aihua,Qi, Rong,Wang, Cheng,Qin, Zhiyong,Fang, Ming,Liu, Jiaqi,Xia, Changquan,Li, Ruxin,Xu, Zhizhan American Institute of Physics 2016 Physics of plasmas Vol.23 No.5