Effects of Replacing Sucrose with Various Sugar Alcohols on Quality Properties of Semi-dried Jerky

Sung Jin Jang,Hyun Wook Kim,Ko Eun Hwang,Dong Heon Song,Yong Jae Kim,Youn Kyung Ham,Yun Bin Lim,Tae Jun Jeong,Si Young Kim 한국축산식품학회 2015 한국축산식품학회지 Vol.35 No.5

The objective of this study was to investigate the effects of replacing sucrose with sugar alcohols (sorbitol, glycerol and xylitol) on the quality properties of semi-dried jerky. Total 7 treatments of jerkies were prepared as follows: control with sucrose, and treatments with 2.5 and 5.0% of sucrose replaced by each sugar alcohol, respectively. Drying yield, pH, water activity, moisture content, shear force, myofibrillar fragmentation index (MFI), 2-thiobarbituric acid reactive substance (TBARS) value, sugar content, and sensory evaluation were evaluated. Xylitol slightly decreased the pH when compared to the other sugar alcohols (p>0.05). The water activity of the semidried jerky was significantly reduced by treatment with glycerol and xylitol (p<0.05). The moisture content of semi-dried jerky containing various sugar alcohols was significantly higher than that of the control (p<0.05), while replacing sucrose with glycerol yielded the highest moisture content. The shear force of semi-dried jerky containing sugar alcohols was not significantly different for the sorbitol and glycerol treatments, but that replacing sucrose with 5.0% xylitol demonstrated the lowest shear force (p<0.05). The TBARS values of semi-dried jerkies with sugar alcohols were lower than the control (p<0.05). The sugar content of the semi-dried jerkies containing sorbitol and glycerol were lower than the control and xylitol treatment (p<0.05). In comparison with the control, the 5.0% xylitol treatment was found to be significantly different in the sensory evaluation (p<0.05). In conclusion, semi-dried jerky made by replacement with sugar alcohols improved the quality characteristics, while xylitol has applicability in manufacturing meat products.

Prognostic factors associated with the success rates of posterior orthodontic miniscrew implants

Sung-Bin Hong,Budi Kusnoto,Eun-Jeong Kim,Ellen A BeGole,Hyeon-Shik Hwang,Hoi-Jeong Lim 대한치과교정학회 2016 대한치과교정학회지 Vol.46 No.2

Objective: To systematically review previous studies and to assess, via a subgroup meta-analysis, the combined odds ratio (OR) of prognostic factors affecting the success of miniscrew implants (MIs) inserted into the buccal posterior region. Methods: Three electronic searches that were limited to articles on clinical human studies using MIs that were published in English prior to March 2015 were conducted. The outcome measure was the success of MIs. Patient factors included age, sex, and jaw of insertion (maxilla vs. mandible), while the MI factors included length and diameter. A meta-analysis was performed on 17 individual studies. The quality of each study was assessed for non-randomized studies and quantified using the Newcastle-Ottawa Scale. The meta-analysis outcome was a combined OR. Subgroup and sensitivity analyses based on the study design, study quality, and sample size of miniscrews implanted were performed. Results: Significantly higher success rates were revealed for MIs inserted in the maxilla, for patients ≥ 20 years of age, and for long MIs (≥ 8 mm) and MIs with a large diameter (> 1.4 mm). All subgroups acquired homogeneity, and the combined OR of the prospective studies (OR, 3.67; 95% confidence interval [CI], 2.10−6.44) was significantly higher in the maxilla than that in the retrospective studies (OR, 2.10; 95% CI, 1.60−2.74). Conclusions: When a treatment plan is made, these risk factors, i.e. jaw of insertion, age, MI length, and MI diameter, should be taken into account, while sex is not critical to the success of MIs.

Increased prevalence of transfusion-transmitted diseases among people with tattoos: a systematic review and meta-analysis

( Sung Ha Lim ),( Solam Lee ),( Young Bin Lee ),( Chung Hyeok Lee ),( Jong Won Lee ),( Sang Hoon Lee ),( Ju Yeong Lee ),( Joung Soo Kim ),( Mi Youn Park ),( Sang Baek Koh ),( Eung Ho Choi ) 대한피부과학회 2020 대한피부과학회 학술발표대회집 Vol.72 No.1

Background: Whether having a tattoo increases the risk of transfusion transmitted diseases (TTDs) is controversial. Objectives: To identify prevalence and risk of TTDs in people with tattoos as compared with the general population. Methods: A systematic review of the studies published before September 2019 was performed by using the Medline, Embase, and Web of Science databases. Observational studies on hepatitis C virus (HCV), hepatitis B virus (HBV), human immunodeficiency virus (HIV), and syphilis infections in people with and without tattoos were included. Studies that reported disease status without serological confirmation were excluded. Results: A total of 115 studies were quantitatively analyzed. HCV (odd ratio [OR], 2.94; 95% confidence interval [CI], 2.52-3.44), HBV (OR, 1.53; 95% CI, 1.34-1.76), and HIV infections (OR, 3.42; 95% CI, 2.16-5.41) were more prevalent in the tattooed population. In subgroup analyses, after the adjustment of possible publication bias, the prevalence of HCV infection was significantly elevated in the community-dwelling, hospital patient, intravenous (IV) drug user, and prisoner groups. IV drug users and prisoners showed high prevalence rates of HBV infection. The prevalence of HIV infection was significantly increased in the prisoner group. Conclusion: Having a tattoo is associated with an increased prevalence of TTDs. Our approach clarifies in depth and supports a guideline for TTD screening in the tattooed population.

Protein Kinase $C\alpha$ Is Involved in the Cell Condensation During Chondrogenesis in Vitro

Lim, Young-Bin,Kang, Shin-Sung,Lee, young-Sup,Sonn, Jong-Kyung The Korean Society for Integrative Biology 2000 Korean journal of biological sciences Vol.4 No.4

In order to investigate the role of protein kinase C (PKC) in chondrogenic differentiation, we examined the localization of PKC isoforms in a limb bud micromass culture system. PKC$\alpha$ is specifically localized in the regions which would become cartilage nodules, while PKC$\lambda/l$ and $\zeta$ display widespread distribution in the whole culture. Distribution of PKC$\alpha$ change along with promotion or inhibition of chondrogenesis by lysophosphatidylcholine or phorbol 12-myristate 13-acetate. On the other hand, localization of PKC$\lambda/l$ or $\zeta$ a was not changed by the modulation of chondrogenesis. Peanut agglutinin binding protein which is associated with cell aggregation during chondrogenesis was present in the cell condensation regions and its expression in those regions was influenced by PKC activity. Expression of fibronectin and N-cadherin in the cell condensing area were also affected by modulation of PKC activity. These results suggest involvement of PKC$\alpha$ in the cell condensation, possibly through regulating expression of fibronectin and N-cadherin.

Characterization of Echinostoma cinetorchis endoribonuclease, RNase H

Sung-Bin Lim,Seok Ho Cha,Seung Jegal,Hojong Jun,Seo Hye Park,Bo-Young Jeon,Jhang Ho Pak,Young Yil Bakh,Tong-Soo Kim,Hyeong-Woo Lee 대한기생충학열대의학회 2017 The Korean Journal of Parasitology Vol.55 No.4

Echinostoma cinetorchis is an oriental intestinal fluke causing significant pathological damage to the small intestine. The aim of this study was to determine a full-length cDNA sequence of E. cinetorchis endoribonuclease (RNase H; EcRNH) and to elucidate its molecular biological characters. EcRNH consisted of 308 amino acids and showed low similarity to endoribonucleases of other parasites (<40%). EcRNH had an active site centered on a putative DDEED motif instead of DEDD conserved in other species. A recombinant EcRNH produced as a soluble form in Escherichia coli showed enzymatic activity to cleave the 3-O-P bond of RNA in a DNA-RNA duplex, producing 3-hydroxyl and 5-phosphate. These findings may contribute to develop antisense oligonucleotides which could damage echinostomes and other flukes.

Development and Validation of HPLC method for Determination of L-Carnitine L-Tartrate in Functional Food

Sung Cheol Shin,Ji Yeon Kim,Sang Jun Cha,Jung Soo Park,Jin Yi Seol,Ju Young Lee,Jung Eun Lee,Sung Bin Park,Sook Jin Jeong,Dong Gil Lim,Chang Hee Lee 한국식품영양과학회 2015 한국식품영양과학회 학술대회발표집 Vol.2015 No.8

Differential Effect of MyD88 Signal in Donor T Cells on Graft-versus-Leukemia Effect and Graft-versus-Host Disease after Experimental Allogeneic Stem Cell Transplantation

Lim, Ji-Young,Ryu, Da-Bin,Lee, Sung-Eun,Park, Gyeongsin,Choi, Eun Young,Min, Chang-Ki Korean Society for Molecular and Cellular Biology 2015 Molecules and cells Vol.38 No.11

Despite the presence of toll like receptor (TLR) expression in conventional $TCR{\alpha}{\beta}$ T cells, the direct role of TLR signaling via myeloid differentiation factor 88 (MyD88) within T lymphocytes on graft-versus-host disease (GVHD) and graft-versus-leukemia (GVL) effect after allogeneic stem cell transplantation (allo-SCT) remains unknown. In the allo-SCT model of C57BL/6 ($H-2^b$) ${\rightarrow}$ B6D2F1 ($H-2^{b/d}$), recipients received transplants of wild type (WT) T-cell-depleted (TCD) bone marrow (BM) and splenic T cells from either WT or MyD88 deficient (MyD88KO) donors. Host-type ($H-2^d$) P815 mastocytoma or L1210 leukemia cells were injected either subcutaneously or intravenously to generate a GVHD/GVL model. Allogeneic recipients of MyD88KO T cells demonstrated a greater tumor growth without attenuation of GVHD severity. Moreover, GVHD-induced GVL effect, caused by increasing the conditioning intensity was also not observed in the recipients of MyD88KO T cells. In vitro, the absence of MyD88 in T cells resulted in defective cytolytic activity to tumor targets with reduced ability to produce IFN-${\gamma}$ or granzyme B, which are known to critical for the GVL effect. However, donor T cell expansion with effector and memory T-cell differentiation were more enhanced in GVHD hosts of MyD88KO T cells. Recipients of MyD88KO T cells experienced greater expansion of Foxp3- and IL4-expressing T cells with reduced INF-${\gamma}$ producing T cells in the spleen and tumor-draining lymph nodes early after transplantation. Taken together, these results highlight a differential role for MyD88 deficiency on donor T-cells, with decreased GVL effect without attenuation of the GVHD severity after experimental allo-SCT.

A Preliminary Study on the Potential Protective Role of the Antioxidative Stress Markers of Cognitive Impairment: Glutathione and Glutathione Reductase

Sang-a Park,Gihwan Byeon,Jin Hyeong Jhoo,Hyung-Chun Kim,Myoung-Nam Lim,Jae-Won Jang,Jong Bin Bae,Ji Won Han,Tae Hui Kim,Kyung Phil Kwak,Bong Jo Kim,Shin Gyeom Kim,Jeong Lan Kim,Seok Woo Moon,Joon Hyuk 대한정신약물학회 2023 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.21 No.4

Objective: To investigate the relationship between reduced glutathione (GSH), a key molecule of the antioxidant defense system in the blood, and glutathione reductase (GR), which reduces oxidized glutathione (glutathione disulfide [GSSG]) to GSH and maintains the redox balance, with the prevalence of Alzheimer’s dementia and cognitive decline. Methods: In all, 20 participants with Alzheimer’s dementia who completed the third follow-up clinical evaluation over 6 years were selected, and 20 participants with normal cognition were selected after age and sex matching. The GSH and GR concentrations were the independent variables. Clinical diagnosis and neurocognitive test scores were the dependent variables indicating cognitive status. Results: The higher the level of GR, the greater the possibility of having normal cognition than of developing Alzheimer’s dementia. Additionally, the higher the level of GR, the higher the neurocognitive test scores. However, this association was not significant for GSH. After 6 years, the conversion rate from normal cognition to cognitive impairment was significantly higher in the lower 50th percentile of the GR group than in the upper 50th percentile. Conclusion: The higher the GR, the lower the prevalence of Alzheimer’s dementia and incidence of cognitive impairment and the higher the cognitive test scores. Therefore, GR is a potential protective biomarker against Alzheimer’s dementia and cognitive decline.

Seedling Conditions for Kimchi Cabbage, Head Lettuce, Cabbage and Broccoli for a Riding-type Transplanter

Lim, Jung-Hyun,Park, Suh-young,Chae, Won-Byung,Kim, Sung-Kyeom,Choi, Seung-Kook,Yang, Eun-Young,Lee, Min-Ji,Park, Gyeong-Bin,Jang, Yoon-ah,Seo, Myeong-Hoon,Jang, Suk-Woo Korean Society for Agricultural Machinery 2017 바이오시스템공학 Vol.42 No.2

Purpose: We have studied the necessary qualities of seedlings of some leafy vegetables for a riding-type two-row automatic transplanter. When seedlings are planted using a transplanter, long roots may be rounded in the bottom of the tray, and this can interfere with the separation of the seedlings. Uprightness related to leaf spread angle is an important quality for seedlings in mechanical planting. Methods: To select cultivars suitable for the transplanter, we compared varieties of Kimchi cabbage (Chukwang, Daetong, Whipalam and Namdo), head lettuce (Abi and Sensation), cabbage (YR Onnuri, YR Hogel, Harutama, and Ogane), and broccoli (Nicegreen and Earlyyou). To compare the effect of bed soil on root formation and growth, we used five types of soil: Chologi, Burger, Wonjomix, Bio, and Baroker with 2.6-3 L per tray. Growth increment and the degree of root formation were measured according to the RDA guidelines 25 days after sowing for Kimchi cabbage and head lettuce and 44 days after sowing for cabbage and broccoli. Conclusions: According to the plug tray, the optimum seedling age in both 128- and 200-hole trays was 28 days for Kimchi cabbage and 44 days for cabbage and broccoli. Head lettuce took 35 days in 128-hole trays and 31 days in 200-hole trays. Burger soil was most effective for root formation and growth of the four kinds of leafy vegetables; it appeared that smaller soil volume led to faster root formation.

Protective Effect of Morin on the Imipenem-induced Nephrotoxicity in Rabbits

Lim, Sung-Chul,Im, Young-Bin,Bae, Chun-Sik,Han, Song-Iy,Kim, Se-Eun,Han, Hyo-Kyung 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.8

The present study investigated the protective effect of morin, a natural flavonoid, on the imipenem-induced nephrotoxicity in rabbits. Nephrotoxicity of imipenem was examined after the intravenous administrations of imipenem (200 mg/kg) to rabbits in the presence and the absence of morin (12, 25, 50 mg/kg, p.o.). Cytotoxicity of imipenem was also examined in the presence and the absence of morin ($100\;{\mu}M$) by using MDCK cells overexpressing human organic anion transporter 1 and 3 (MDCK/hOAT1 or MDCK/hOAT3). Intravenous dosing of imipenem alone induced severe proximal tubular necrosis in rabbits, however, the concurrent use of morin (25 or 50 mg/kg, p.o.) significantly suppressed the histopathological damage in the kidney induced by imipenem. While imipenem was not cytotoxic in MDCK/hOAT1 cells over the tested concentrations up to 10 mM, it showed significant cellular toxicity with $CC_{50}$ of 0.77 mM in MDCK/hOAT3 cells, implying that OAT3 may involve more actively in the imipeneminduced nephrotoxicity. In addition, the cellular toxicity of imipenem decreased by approximately 20 folds in the presence of morin in MDCK/hOAT3 cells. In conclusion, the present study suggests that morin might be beneficial to reduce the nephrotoxicity of imipenem, at least in part, via the inhibition of OAT3-mediated renal excretion of imipenem.