Dammarane-type triterpene oligoglycosides from the leaves and stems of Panax notoginseng and their antiinflammatory activities

Li, Juan,Wang, Ru-Feng,Zhou, Yue,Hu, Hai-Jun,Yang, Ying-Bo,Yang, Li,Wang, Zheng-Tao The Korean Society of Ginseng 2019 Journal of Ginseng Research Vol.43 No.3

Background: Inflammation is widespread in the clinical pathology and closely associated to the progress of many diseases. Triterpenoid saponins as a key group of active ingredients in Panax notoginseng (Burk.) F.H. Chen were demonstrated to show antiinflammatory effects. However, the chemical structures of saponins in the leaves and stems of Panax notoginseng (PNLS) are still not fully clear. Herein, the isolation, purification and further evaluation of the antiinflammatory activity of dammarane-type triterpenoid saponins from PNLS were conducted. Methods: Silica gel and reversed-phase C8 column chromatography were used. Furthermore, preparative HPLC was used as a final purification technique to obtain minor saponins with high purities. MS, NMR experiments, and chemical methods were used in the structural identifications. The antiinflammatory activities of the isolated saponins were assessed by measuring the nitric oxide production in RAW 264.7 cells stimulated by lipopolysaccharides. Real-time reverse transcription polymerase chain reaction was used to measure the gene expressions of inflammation-related gene. Results: Eight new minor dammarane-type triterpene oligoglycosides, namely notoginsenosides LK1-LK8 (1-8) were obtained from PNLS, along with seven known ones. Among the isolated saponins, gypenoside IX significantly suppressed the nitric oxide production and inflammatory cytokines including tumor necrosis $factor-{\alpha}$, interleukin 10, interferon-inducible protein 10 and $interleukin-1{\beta}$. Conclusion: The eight saponins may enrich and expand the chemical library of saponins in Panax genus. Moreover, it is reported for the first time that gypenoside IX showed moderate antiinflammatory activity.

Curdione Inhibits Proliferation of MCF-7 Cells by Inducing Apoptosis

Li, Juan,Bian, Wei-He,Wan, Juan,Zhou, Jing,Lin, Yan,Wang, Ji-Rong,Wang, Zhao-Xia,Shen, Qun,Wang, Ke-Ming Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Background: Curdione, one of the major components of Curcuma zedoaria, has been reported to possess various biological activities. It thus might be a candidate anti-flammatory and cancer chemopreventive agent. However, the precise molecular mechanisms of action of curdione on cancer cells are still unclear. In this study, we investigated the effect of curdione on breast cancer. Materials and Methods: Xenograft nude mice were used to detect the effect of curdione on breast cancer in vivo; we also tested the effect of curdione on breast cancer in vitro by MTT, Flow cytometry, JC-I assay, and western blot. Results: Firstly, we found that curdione significantly suppressed tumor growth in a xenograft nude mouse breast tumor model in a dose-dependent manner. In addition, curdione treatment inhibited cell proliferation and induced cell apoptosis. Moreover, after curdione treatment, increase of impaired mitochondrial membrane potential occurred in a concentration dependent manner. Furthermore, the expression of apoptosis-related proteins including cleaved caspase-3, caspase-9 and Bax was increased in curdione treatment groups, while the expression of the anti-apoptotic Bcl-2 was decreased. Inhibitors of caspase-3 were used to confirm that curdione induced apoptosis. Conclusions: Overall, our observations first suggested that curdione inhibited the proliferation of breast cancer cells by inducing apoptosis. These results might provide some molecular basis for the anti-cancer activity of curdione.

Comprehensive transcriptome and metabolome analysis reveals regulatory network for lignin biosynthesis in chicory sprouting

Li Juan,Guo Xiaozhu,Wang Gui,Zhou Zhenjie,Sun Xuemei 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.5

Chicory is consumed worldwide and is an important commercial crop. However, excess lignin deposition may reduce its quality. The comprehension of the molecular mechanism underlying the biosynthesis of lignin in chicory is currently inadequate. To address this, an integrative analysis of the metabolome and transcriptome profiles was performed in chicory sprout at three different stages. A total of 706 metabolites were identified, with cinnamic acid, ferulic acid, coniferaldehyde, and sinapaldehyde enriched during the growth of chicory sprouts. This suggested that these four metabolites may affect the growth of chicory sprouts. Transcriptome analysis demonstrated that the expression of most of the differentially expressed genes (DEGs) involved in lignin biosynthesis was up-regulated during chicory growth. Importantly, the metabolite and gene expression profiles were closely correlated during sprout development, especially in association with lignin biosynthesis. The results will serve as a reference for lignin biosynthesis in chicory and may also assist biologists in improving chicory quality.

Selection of Salt-tolerant Strawberry Cultivars by Assessing Germination under Excess NaCl and Seawater Conditions

Lijuan Zhang,Sayaka Kitanishi,Yuichi Uno,Michio Kanechi,Noboru Inagaki 한국원예학회 2009 Horticulture, Environment, and Biotechnology Vol.50 No.5

A Study on the Destination Preferences of Chinese Tourists in Seoul and Jeju Island

Lijuan Che,Haengnam Sung,Byung-Hyuk Ahn 한국인터넷전자상거래학회 2015 인터넷전자상거래연구 Vol.15 No.5

This study aims to find out the behavior of Chinese tourists in Korea. Understanding the behavior of Chinese tourists may help Korean government and the related industry set up their tourism strategies and policies for Chinese tourists. We want to know when Chinese tourists travel to two major places(Seoul and Jeju Island) and what causes the different level of tourists’ satisfaction. Results of the study were as follows: First, Tourists visiting Seoul have higher intention of experiencing different lifestyle and shopping while those visiting Jeju Island show preferences to outdoor activity. Second, both destination image and travel purpose are significantly influence the overall destination attribute satisfaction. Third, tourists’ satisfaction of overall destination attributes positively influences their loyalties to the destination. Fourth, different travel purposes(e.g. different lifestyle, shopping/beauty, outdoor activity) have different significance influences on destination attribute satisfaction(e.g. food, event/entertainment, environment, shopping). The result of this research may help as to know more about the Chinese tourists’ behavior and make some suggestions to attract more Chinese tourists.

A Ultra-Low Specific On-Resistance and Extended Gate SJ LDMOS Structure

Lijuan Wu,Jiaqi Chen,Hang Yang,QiLin Ding,Xing Chen,Shaolian Su 한국전기전자재료학회 2021 Transactions on Electrical and Electronic Material Vol.22 No.3

A new structure of extended gate (EG) SJ LDMOS is proposed in this paper to overcome the substrate assisted depletion (SAD) eff ect in the structure of Super-Junction lateral double diff used metal oxide semiconductor (SJ LDMOS). Different from other surface SJ structures, the SJ layer of the structure is located in the body of the drift region. Gate oxide and silicon layer form the EG Structure-Oxide-Semiconductor structure that is similar to Metal–Insulator–Semiconductor capacitor. The the N-drift of the EG structure can obtain the charge compensation to overcome the SAD eff ect, and a nearly rectangular electric field is achieved. In the on-state, the EG structure has two conductive channels and the accumulation layer is formed on the drift region. By accumulating high concentration electrons in the channels, the specifi con-resistance ( R ON,sp ) is greatly reduced. When the drift length is 24 μm, 431.4 V breakdown voltage ( V B ) and 9.8 mΩ cm 2 R ON,sp are achieved, and the fi gure of merit is 18.9 MW cm −2 .

Screening a Panel of Acid-producing Strains by Developing a High-throughput Method

Lijuan Zhu,Hui Zhang,Shiyuan Wang,Anqi Zhao,Lingbo Qu,Wenlong Xiong,Md. Asraful Alam,Wenlong Ma,Yongkun Lv,Jingliang Xu 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.5

Organic acids are natural cellular metabolites, which are widely used in food, pharmaceutical, and chemical industries. Among them, L-lactic acid is of special interest, because it is widely used in food and pharmaceutical industries and its monopolymer (poly (lactic acid)) is a green, renewable, biodegradable, and biocompatible alternative to the petroleum-based polymers. Currently, organic acids are predominantly produced by microbial fermentation. Their productions have been substantially improved by genetic modifications, metabolic engineering, and fermentation optimizations. However, the commonly used microbial producers still suffer from low acidic tolerance. Screening higher tolerant acid-producing microorganisms from the nature is relatively less explored. The traditional fermented foods are good resources for the screening of acid-producing and probiotic microorganisms. However, they are relatively less explored, especially those foods in developing countries. To speed up the acid-producing microorganism screening, we developed and validated a high-throughput method in this study. By using this method, we screened 1,296 colonies in 4 days and obtained a panel of acid-producing microorganisms. Among them, a Lacticaseibacillus rhamnosus showed the potential for organic acid production and probiotics applications.

A novel multiemissive Ln/covalent-organic frameworks for ratiometric detection of 2,6-dipicolinic acid

Lijuan Kuang,Yue Du,Shiqi Wang,Li Yang,Yonghai Song 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.106 No.-

As one of the specific biomarkers of anthrax, timely, sensitive and accurate detection of 2,6-dipicolinicacid (DPA) plays an important role in preventing biological weapons attacks and disease outbreaks. Here, multiemission Eu3+/covalent- organic framework (COFDTA-TFP) and Tb3+/COFDTA-TFP were constructedby the coordination between Eu3+ or Tb3+ with fluorescent COFDTA-TFP which was prepared byamine-aldehyde dehydration condensation between 2,5-diaminoterephthalic acid and 2,4,6-triformylphloroglucinol for the first time. The Eu3+/COFDTA-TFP and Tb3+/COFDTA-TFP can emit nonoverlappingfluorescence of COFDTA-TFP and Eu3+ or Tb3+, which was used to ratiometric detection ofDPA. Due to the strong coordination between the nitrogen atoms in the pyridine ring and the oxygenatoms in the carboxyl group of DPA with Eu3+ or Tb3+, DPA replaced coordinated H2O to sensitize fluorescenceof Eu3+ or Tb3+ by ‘‘antenna” effect but fluorescence of COFDTA-TFP with two-dimensional lamellarstructure was kept constant as a reference. The linear range and detection limit of ratiometric fluorescencesensor based on Eu3+/COFDTA-TFP for detection of DPA are 0.01–12 mM and 4.2 nM, respectively. The linear range and detection limit of ratiometric fluorescence sensor based on Tb3+/COFDTA-TFP fordetection of DPA are 0.01–9.0 mM and 2.9 nM, respectively.

Acid–base bifunctional catalysis by a heteropolyacid and amines on the polyetheretherketone fiber for cleaner acceleration of the one-pot tandem reactions

Lijuan Jiang,Xian-Lei Shi,Yue Lv,Honghui Gong,Shuangshuang Liu,Mengmeng Du,Qianqian Hu,Keren Shi 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.113 No.-

The development of highly efficient, economical and environmentally friendly catalytic systems is ofgreat significance from the green chemistry point of view. In this paper, we presented a succinct approachto create a heterogeneous acid–base bifunctional catalyst for one-pot tandem reaction from the commerciallyavailable textile fiber. The ultra-high strength textile fiber polyetheretherketone (PEEK) was functionalizedby a post-grafting method to combine two antagonistic active functions in a synergisticcatalyst, and the resulting fiber samples were characterized in detail by morphology, mechanical properties,elemental analysis, X-ray photoelectron spectroscopy, inductively coupled plasma-atomic emissionspectrometry, X-ray diffraction, Fourier transform infrared spectroscopy, ultraviolet–visible spectrum,nuclear magnetic resonance spectroscopy and thermogravimetric analysis, and further revealed thatthe amines and the heteropolyacid were immobilized by acid–base interactions in the PEEK surface layerwith sufficient stability. Moreover, the acid–base bifunctional catalyst can be successfully applied in theacceleration of the one-pot tandem deacetalization–Knoevenagel reactions with high-efficiency (lowercatalyst dosage 0.3 mol%, higher product yields 81–92%), whereas the homogeneous catalysts wereunable to initiate the reaction due to their mutual neutralization in solution, and the catalytic mechanismwas elucidated by comparison. Furthermore, the fibrous catalyst could maintain its activities more than10 cycles with a simple post-processing, and the mediated system was capable of enlarging to the gramscale,which are envisaged for industrial operations and cleaner productions.

Motivation of College Students1 Sports Tourism Participation in Shanghai: Probing Gender Differences

Lijuan Wang 한국서비스디자인학회 2023 한국서비스디자인학회 학술대회자료집 Vol.2023 No.1