Protective effect of liensinine on periodontitis through its antioxidant effect in mice

Liang Pang,Kai Zhu,Xia Feng,Weiwei Liu,Deguang Peng,Lihua Qiu,Xiang Gao,Jiang Deng,Ying Li,Xin Zhao 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.6

Liensinine is the most important functional compound in lotus seeds; its protective effect on periodontitis was determined and the mechanism of its antioxidant effect was also explained. During the experiment, we could observe that liensinine could reduce the gingival index in periodontitis-induced mice, and these effects were similar to that during the treatment of periodontitis with the metronidazole drug. After the treatment with liensinine, the serum Immunoglobulin G1 (IgG1) level was found to increase and IgG2a level was found to decrease compared to the periodontitis control mice. Liensinine and metronidazole could increase the superoxide dismutase (SOD) serum levels in the periodontitis-induced mice, and both the drugs at the same concentration (200 mg/kg) showed similar SOD activities, which was found to increase. Liensinine also increased the oxidationrelated factor of catalase (CAT), glutathione peroxidase, and decreased nitric oxide, malondialdehyde, and endothelin in the serum of mice, compared to the periodontitis control mice. The serum cytokine levels of Interleukin-6, tumor necrosis factor-a, and Interferon-c in liensinine-treated mice were lower than the control mice, but higher than the normal mice. The serum IL-4 cytokine level of liensinine-treated mice was reversed, i.e., higher than the control mice. In the periodontal tissue, the mRNA and protein expressions of inhibitor of kappa B-a, transforming growth factor beta 1, Interleukin-10, Mn-SOD, Gu/Zn-SOD, and CAT were increased by the treatment with liensinine compared to the control group mice, and nuclear factor kappa B expressions showed the opposite trend. Liensinine is a good functional compound in the treatment of periodontitis, similar to metronidazole drug. Higher concentration of liensinine had better effects.

Protective effect of liensinine on periodontitis through its antioxidant effect in mice

Pang, Liang,Zhu, Kai,Feng, Xia,Liu, Weiwei,Peng, Deguang,Qiu, Lihua,Gao, Xiang,Deng, Jiang,Li, Ying,Zhao, Xin 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.6

Liensinine is the most important functional compound in lotus seeds; its protective effect on periodontitis was determined and the mechanism of its antioxidant effect was also explained. During the experiment, we could observe that liensinine could reduce the gingival index in periodontitis-induced mice, and these effects were similar to that during the treatment of periodontitis with the metronidazole drug. After the treatment with liensinine, the serum Immunoglobulin G1 ($IgG_1$) level was found to increase and $IgG_{2a}$ level was found to decrease compared to the periodontitis control mice. Liensinine and metronidazole could increase the superoxide dismutase (SOD) serum levels in the periodontitis-induced mice, and both the drugs at the same concentration (200 mg/kg) showed similar SOD activities, which was found to increase. Liensinine also increased the oxidation-related factor of catalase (CAT), glutathione peroxidase, and decreased nitric oxide, malondialdehyde, and endothelin in the serum of mice, compared to the periodontitis control mice. The serum cytokine levels of Interleukin-6, tumor necrosis factor-${\alpha}$, and Interferon-${\gamma}$ in liensinine-treated mice were lower than the control mice, but higher than the normal mice. The serum IL-4 cytokine level of liensinine-treated mice was reversed, i.e., higher than the control mice. In the periodontal tissue, the mRNA and protein expressions of inhibitor of kappa B-${\alpha}$, trans-forming growth factor beta 1, Interleukin-10, Mn-SOD, Gu/Zn-SOD, and CAT were increased by the treatment with liensinine compared to the control group mice, and nuclear factor kappa B expressions showed the opposite trend. Liensinine is a good functional compound in the treatment of periodontitis, similar to metronidazole drug. Higher concentration of liensinine had better effects.

Research on Tangential Magnetization Parallel Magnetic Path Hybrid Excitation Synchronous Machine

Pang Liang,Zhao ChaoHui,Shen HeBiao 대한전기학회 2022 Journal of Electrical Engineering & Technology Vol.17 No.5

A hybrid excitation motor with strong magnetizing ability is proposed. To solve the existing hybrid excitation machine problems, such as the diffi culty of total adjustment of air gap magnetic fi eld, many additional magnetic paths, fault tolerance is poor, and Air gap fl ux density harmonic distortion rate is high. According to the structural characteristics and the principle of magnetic regulation of tangential magnetization parallel magnetic path hybrid excitation synchronous machine. The equivalent magnetic path model of the motor was established, and the infl uence of electric excitation magnetic potential on permanent magnetic potential and air gap magnetic fi eld was analyzed. With the ANSYS Maxwell fi nite element simulation software, the variation law of the magnetic fi eld of the motor under diff erent excitation conditions is studied. The infl uence of pole boot length and magnetic pole eccentricity on air gap fl ux density amplitude and harmonic distortion rate is discussed. The results show that due to the characteristics of the parallel magnetic path. The motor has obvious eff ect of adjusting magnetic fi eld and gathering magnetic fi eld, motor fault tolerance is excellent, and less additional magnetic path. In addition, the air gap density can be optimized by using reasonable pole boot length and magnetic pole eccentricity.

Molecular Cloning and Expression of Forkhead Transcription Factor O1 Gene from Pig Sus scrofa

Pang, Weijun,Sun, Shiduo,Bai, Liang,Yang, Gongshe Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.4

Foxo1 plays an important role in the integration of hormone-activated signaling pathways with the complex transcriptional cascade that promotes preadipocyte differentiation of clonal cell lines from rodents. We isolated the full-length cDNA of porcine FoxO1 gene using RACE, confirmed by visual Northern blotting. The deduced amino acids indicated 94% and 90% identities with the corresponding human and mice aa. Analysis of the aa sequence, showed that it included a Forkhead domain (aa 167-247), a transmembrane structure domain (aa 90-113), a LXXLL motif (aa 469-473), and 51 Ser, 8 Thr, and 4 Tyr phosphorylation sites, indicating a potential important role for FoxO1 transcriptional activity in vivo. Using the IMpRH panel, we mapped FoxO1 gene to chromosome 11p13. Our data provide basic molecular information useful for the further investigation on the function of FoxO1 gene. Time-course analysis of FoxO1 expressions indicated that levels of mRNA and protein gradually increased from day 0 to 3, and it reached almost maximal level at day 3, then decreased from day 5 to 7 in porcine primary preadipocyte differentiation. After induction by IGF-1, GPDH activity and accumulation of lipid increased, however, expressions of FoxO1 mRNA and protein were inhibited in a dose dependent manner. These results suggest that FoxO1 takes part in porcine preadipocyte differentiation and expressions of FoxO1 were regulated by IGF-1.

The Investment Always Will Get Gains? Advertising Expenditure and Enterprise Performance Based on Corporate Life Cycle

Li, Liang,Amine, Bouirig,Pang, Yuxin,Jiang, Minxing Kyung Hee University Management Research Institute 2022 Asia pacific journal of business review Vol.6 No.2

Based on the concept of advertising expenditure, manufacturing firm performance, and enterprise life cycle, this research conducts the listed Chinese manufacturing listed in 2016-2018 as the research sampling and divides these listed companies into three periods: growth, maturity, and decline. Next, this paper conducts empirical research from three aspects: advertising expenditure or investment and manufacturing firm performance, lag effect of advertising expenditure, and outcome effect and lag effect of advertising investment. It is found that in different stages of manufacturing enterprises, different advertising expenditures will have different impacts on the performance of manufacturing enterprises. In the growth stage, the advertising investment of manufacturing enterprises will significantly affect the performance results of the current period, and there will be the long-term lag effect. In contrast, the mature stage of enterprises' advertising investment has a shorter period of lag effect, while in the recession stage, the mature stage of manufacturing enterprises' advertising investment will have a shorter period of lag effect. The empirical results are not significant. This study provides a reference for manufacturing enterprises in different stages in the decision-making of advertising investment.

Localized surface plasmon resonance enhanced by the light-scattering property of silver nanoparticles for improved luminescence of polymer light-emitting diodes

Cheng-Liang Huang,Hung Ji Huang,Sy-Hann Chen,Yu-Siang Huang,Po-Ching Kao,Yuan-Fong Chou Chau,Hai-Pang Chiang 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.103 No.-

This study used photochemical reduction to successfully synthesize triangular silver nanoplates (TAgNPs)and silver nanodecahedrons (AgNDs) with higher light-absorption and higher light-scattering properties,respectively, for the same wavelength. To analyze the contribution of light-absorption and lightscatteringof silver nanoparticles to the localized surface plasmon resonance (LSPR) effect, poly(3,4-ethylenedioxythiophene) polystyrene sulfonate was doped with TAgNPs or AgNDs at the same concentration(1.17 lg/cm2) and made into polymer light-emitting diodes (PLEDs). According to the current densityvoltageluminancecharacteristics and electroluminescence (EL) spectra, the enhancement factors for currentefficiency and EL intensity for AgND-containing PLEDs were found to be higher than those for PLEDwith TAgNPs by 24.9% and 138%, respectively. This shows that the metal nanoparticles with higher lightscatteringproperty can induce a relatively strong LSPR effect, which possibly gives a hint to design plasmonicphotovoltaic in future.

The enhancement of oxygen sensitivity of ZnO macropore film by functionalizing with azo pigment

Peng, Liang,Zhai, Jiali,Wang, Dejun,Zhang, Yu,Zhao, Qidong,Wang, Ping,Pang, Shan,Fan, Zhiyong,Xie, Tengfeng Korean Society of Photoscience 2009 Photochemical & photobiological sciences Vol.8 No.6

Azo-ZnO hybrid films were prepared by functionalizing the ZnO macropore films with azo pigment (1,1'-(biphenyl-4,4'-diylbis(diazene-2,1-diyl))dinaphthalen-2-ol). Scanning electronic microscopy, current-voltage, UV-Vis absorption spectroscopy and Kelvin probe were used to characterize these films. The oxygen sensing characteristics of hybrid films and pure ZnO film were measured under the irradiation of UV light. The results show that the sensitivity of hybrid film is about 500 times higher than that of pure ZnO film. The high sensitivity to oxygen of hybrid films is mainly attributed to the increasing of the photo-generated electron concentration. Enhanced photo-induced charge separation after functionalization is confirmed by the pattern of transient photovoltage. Our results demonstrate that the functionalization with azo pigment is a promising approach to enhance the oxygen sensitivity of ZnO under the irradiation of UV light.

Effective of high salt diet (HSD) on letrozole-induced polycystic ovarian syndrome (PCOS) mice

Suying Liang,Xiaoying Tang,Minmin Chen,Huiwen Liu,Wanying Li,Zixian Wang,Qi Qi Pang,Jia-Le Song 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

To investigate the effect of high salt diet (HSD, 8% NaCl) on the letrozole induced PCOS mice. At the total of experimental periods (35 days), all female C57BL/6J mice were randomly divided into normal groups, PCOS group (ingested letrozole daily for 21 days) and HSD+PCOS group. The body weight (BW) and histologic changes were evaluated. The serum levels of testosterone (T), LH, E2, FSH, INS, TG, TC, LDL, HDL were determined by ELISA kits. Compared with normal groups, the BW, T, LH, INS, TG, TC LDL-C in PCOS rats were significantly increased, and the levels of E2, FSH and HDL-C were significantly decreased. In addition, compared with the PCOS group, the BW, T, LH, INS, TG, TC LDL-C in HSD+PCOS rats were significantly increased, and the levels of E2, FSH and HDL-C were significantly decreased. Our results showed that HSD exacerbates metabolic disorders and hormonal imbalances in PCOS mice.

3-Deazaneplanocin A is a Promising Therapeutic Agent for Ovarian Cancer Cells

Shen, Liang,Cui, Jing,Pang, Ying-Xin,Ma, Yan-Hui,Liu, Pei-Shu Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Background: Recent studies have shown that 3-deazaneplanocin A (DZNep), a well-known histone methyltransferase inhibitor, disrupts polycomb-repressive complex 2 (PRC2), and induces apoptosis, while inhibiting proliferation and metastasis, in cancer cells, including acute myeloid leukemia, breast cancer and glioblastoma. However, little is known about effects of DZNep on ovarian cancer cells. Materials and Methods: We here therefore studied DZNep-treated A2780 ovarian cancer cells in vitro. Proliferation of ovarian cancer cells under treatment of DZNep was assessed by MTT and apoptosis by flow cytometry. Cell wound healing was applied to detect the migration. Finally, we used q-PCR to assess the migration-related gene, E-cadherin. Results: DZNep could inhibit the proliferation of A2780 and induce apoptosis Furthermore, it inhibited migration and increased the expression of E-cadherin (P<0.05). Conclusion: DZNep is a promising therapeutic agent for ovarian cancer cells, with potential to inhibite proliferation, induce apoptosis and decrease migration.

Effects of Multidisciplinary Team-Based Nurse-led Transitional Care on Clinical Outcomes and Quality of Life in Patients with Ankylosing Spondylitis

Limin Liang,Yinghua Pan,DanchunWu,Yongli Pang,Yuanyuan Xie,Hengying Fang 한국간호과학회 2019 Asian Nursing Research Vol.13 No.2

Purpose: The purpose of this study is to investigate the impact of transitional care by a nurse-led multidisciplinary team (MDT) on clinical outcomes and quality of life of patients with ankylosing spondylitis. Methods: A randomized control study design was used. Subjects were allocated randomly to an experimental group and a control group. The experimental group received intensive transitional care by a nurse-led MDT, whereas the control group received routine nursing care. Disease activity, spinal mobility, comprehensive function, health service utilization, and quality of life were assessed at the baseline and at six months with the Bath Ankylosing Spondylitis Metrology Index, the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), the Bath Ankylosing Spondylitis Functional Index (BASFI), a health service utilization questionnaire and version 2 of the Short Form-36 health survey. Results: Compared with the baseline, the BASDAI, BASFI, emergency visits, hospitalizations, hospitalization days, and bodily pain, vitality, mental health, total score, and average score of version 2 of the Short Form-36 health survey were improved in the experimental group (p < .05), whereas only bodily pain, vitality, and role-emotional were improved in the control group (p < .05). At six months, the experimental group exhibited significantly more improvement on the BASDAI, BASFI, hospitalizations, all domains except Role-physical as well as total score and average score (p < .05) compared with the control group. Conclusion: A MDT-based nurse-led transitional care improves clinical outcomes and quality of life of patients with ankylosing spondylitis. Future research should be carried out on modes of follow-up and family support.