Staged Improvement in Awareness of Disease for Elderly Cancer Patients in Southern China

Li, Xing,Dong, Min,Wen, Jing-Yun,Wei, Li,Ma, Xiao-Kun,Xing, Yan-Fang,Deng, Yun,Chen, Zhan-Hong,Chen, Jie,Ruan, Dan-Yun,Lin, Ze-Xiao,Wang, Tian-Tian,Wu, Dong-Hao,Liu, Xu,Hu, Hai-Tao,Lin, Jia-Yu,Li, Zhu Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.15

Background: In mainland China, awareness of disease of elderly cancer patients largely relies on the patients' families. We developed a staged procedure to improve their awareness of disease. Materials and Methods: Participants were 224 elderly cancer patients from 9 leading hospitals across Southern China. A questionnaire was given to the oncologists in charge of each patient to evaluate the interaction between family and patients, patient awareness of their disease and participation in medical decision-making. After first cycles of treatment, increased information of disease was given to patients with cooperation of the family. Then patient awareness of their disease and participation in medical decision-making was documented. Results: Among the 224 cancer elderly patients, 26 (11.6%) made decisions by themselves and 125 (55.8%) delegated their rights of decision-making to their family. Subordinate family members tended to play a passive role in decision-making significantly. Patients participating more in medical decision-making tended to know more about their disease. However, in contrast to the awareness of disease, patient awareness of violation of medical recommendations was reversely associated with their participation in medical decision-making. Improvement in awareness of diagnosis, stages and prognosis was achieved in about 20% elderly cancer patients. About 5% participated more actively in medical decision-making. Conclusions: Chinese elderly cancer patient awareness of disease and participation in medical decision-making is limited and relies on their family status. The staged procedure we developed to improve patient awareness of disease proved effective.

Two new benzylisoquinoline alkaloids from Thalictrum foliolosum and their antioxidant and in vitro antiproliferative properties

Da-Hong Li,Jia Guo,Wen Bin,Nan Zhao,Kai-bo Wang,Jian-yong Li,Zhan-Lin Li,Hui-Ming Hua 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.7

Two novel rare chloro-containing benzylisoquinolinealkaloids, thalfoliolosumines A (1) and B (2),along with eight known isoquinoline alkaloids (3–10) wereisolated from the whole plant of Thalictrum foliolosum. The structures of these compounds were elucidated byspectral analyses, including 1D and 2D NMR (COSY,HSQC, HMBC and NOESY) experiments. The antiproliferativeeffects of all the isolated compounds were evaluatedby MTT method against MCF-7, PC-3, and U937cells, and trypan blue method against HL-60 cells. Newcompounds 1 and 2 exhibited moderate in vitro antiproliferativeactivity against MCF-7, PC-3, and HL-60 cells,and good inhibitory effects against U937 cells with IC50values of 7.50 and 6.97 μM, respectively. Compounds 7and 10 showed the strongest in vitro antiproliferative withIC50 values of 0.93 and 1.69 lM against HL-60 cell line. The antioxidant properties were also measured, bisbenzyltetrahydroisoquinolinealkaloids 3–6 showed the strongestantioxidant activities in ABTS assay.

A Survey of the Geographic Distribution of Ophiocordyceps sinensis

Yi Li,Xiao-Liang Wang,Lei Jiao,Yi Jiang,Hui Li,Si-Ping Jiang,Ngarong Lhosumtseiring,Shen-Zhan Fu,Cai-Hong Dong,Yu Zhan,Yi-Jian Yao 한국미생물학회 2011 The journal of microbiology Vol.49 No.6

Ophiocordyceps sinensis is one of the best known fungi in Traditional Chinese Medicine. Many efforts have been devoted to locating the production areas of this species resulting in various reports; however, its geographic distribution remains incompletely understood. Distribution of O. sinensis at the county level is clarified in this work based on both a literature search and fieldwork. More than 3600 publications related to O. sinensis were investigated, including scientific papers, books, and online information. Herbarium specimens of O. sinensis and field collections made by this research group during the years 2000-2010 were examined to verify the distribution sites. A total of 203 localities for O. sinensis have been found, of which 106 are considered as confirmed distribution sites, 65 as possible distribution sites, 29 as excluded distribution sites and three as suspicious distribution sites. The results show that O. sinensis is confined to the Tibetan Plateau and its surrounding regions, including Tibet, Gansu, Qinghai, Sichuan, and Yunnan provinces in China and in certain areas of the southern flank of the Himalayas, in the countries of Bhutan, India and Nepal, with 3,000 m as the lowest altitude for the distribution. The fungus is distributed from the southernmost site in Yulong Naxi Autonomous County in northwestern Yunnan Province to the northernmost site in the Qilian Mountains in Qilian County, Qinghai Province, and from the east edge of the Tibetan Plateau in Wudu County, Gansu Province to the westernmost site in Uttarakhand, India. The clarification of the geographic distribution of O. sinensis will lay the foundation for conservation and sustainable use of the species.

Xanthones from Garcinia paucinervis with in vitro anti-proliferative activity against HL-60 cells

Da-Hong Li,Chen-Xi Li,Cui-Cui Jia,Ya-Ting Sun,Chun-Mei Xue,Jiao Bai,Hui-Ming Hua,Xiao-Qiu Liu,Zhan-Lin Li 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.2

Three new xanthones, paucinervins H–J (1–3), as well as eleven known compounds (4–14), were isolated from the leaves of Garcinia paucinervis. The structures of the new compounds (1–3) were elucidated by 1D, 2D NMR spectra and HR ESIMS. In vitro antiproliferative activity against human promyelocytic leukemia HL-60 cells was tested, among which, compounds 2, 5, 6 and 7 exhibited strong growth inhibitory effects with GI50 values ranging from 1.30 to 9.08 lM, respectively. Preliminary SARs were also discussed.

Properties and Reaction Mechanisms of Magnesium Phosphate Cement Mixed with Acetic Acid

Li Jun,Ji Yong-sheng,Huang Guo-dong,Xu Zhan-zhan,Yan Gui-hong 대한토목학회 2018 KSCE Journal of Civil Engineering Vol.22 No.1

Objective: This study aims to improve the coagulation time, strength, and construction cost of magnesium phosphate cement. Methods: We analyzed the microstructure changes in the hardened magnesium phosphate cement paste through qualitative analysis, quantitative test, microscopic examination, and research regarding adding different concentrations of acetic acid on magnesium phosphate cement slurry time, effects of flow resistance, and mechanical properties. Results: When a moderate amount of AA was used, the setting time of MAPC mortar was significantly prolonged but was later reduced as the concentration increased. In the early phase, the degree of MAPC mortar hydration increased, whereas compressive strength significantly increased initially and increased slowly in the later phase. The crystal morphology and size of MAPC hydrates changed, and hardened MAPC became more compact in structure. The AA absorbed some ammonia in the MAPC condensation. Conclusion: Acetic acid can effectively regulate coagulation time, induce early hydration reaction rate, and improve the performance of magnesium phosphate cement, which can reduce the construction cost of magnesium phosphate cement.

Efficacy of Prophylactic Entecavir for Hepatitis B Virus-Related Hepatocellular Carcinoma Receiving Transcatheter Arterial Chemoembolization

Li, Xing,Zhong, Xiang,Chen, Zhan-Hong,Wang, Tian-Tian,Ma, Xiao-Kun,Xing, Yan-Fang,Wu, Dong-Hao,Dong, Min,Chen, Jie,Ruan, Dan-Yun,Lin, Ze-Xiao,Wen, Jing-Yun,Wei, Li,Wu, Xiang-Yuan,Lin, Qu Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.18

Background and Aims: Hepatitis B virus (HBV) reactivation was reported to be induced by transcatheter arterial chemoembolization (TACE) in HBV-related hepatocellular carcinonma (HCC) patients with a high incidence. The effective strategy to reduce hepatitis flares due to HBV reactivation in this specific group of patients was limited to lamivudine. This retrospective study was aimed to investigate the efficacy of prophylactic entecavir in HCC patients receiving TACE. Methods: A consecutive series of 191 HBV-related HCC patients receiving TACE were analyzed including 44 patients received prophylactic entecavir. Virologic events, defined as an increase in serum HBV DNA level to more than 1 log10 copies/ml higher than nadir the level, and hepatitis flares due to HBV reactivation were the main endpoints. Results: Patients with or without prophylactic were similar in host factors and the majorities of characteristics regarding to tumor factors, HBV status, liver function and LMR. Notably, cycles of TACE were parallel between the groups. Ten (22.7%) patients receiving prophylactic entecavir reached virologic response. The patients receiving prophylactic entecavir presented significantly reduced virologic events (6.8% vs 54.4%, p=0.000) and hepatitis flares due to HBV reactivation (0.0% vs 11.6%, p=0.039) compared with patients without prophylaxis. Kaplan-Meier analysis illustrated that the patients in the entecavir group presented significantly improved virologic events free survival (p=0.000) and hepatitis flare free survival (p=0.017). Female and Eastern Cooperative Oncology Group (ECOG) performance status 2 was the only significant predictors for virological events in patients without prophylactic antiviral. Rescue antiviral therapy did not reduce the incidence of hepatitis flares due to HBV reactivation. Conclusion: Prophylactic entecavir presented promising efficacy in HBV-related cancer patients receiving TACE. Lower performance status and female gender might be the predictors for HBV reactivation in these patients.

Clinical Study on Safety and Efficacy of Qinin^® (Cantharidin Sodium) Injection Combined with Chemotherapy in Treating Patients with Gastric Cancer

Zhan, Yi-Ping,Huang, Xin-En,Cao, Jie,Lu, Yan-Yan,Wu, Xue-Yan,Liu, Jin,Xu, Xia,Xu, Lin,Xiang, Jin,Ye, Li-Hong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Objectives: To assess the efficacy, side effects, and the impact on quality of life with $Qinin^{(R)}$ (Cantharidin sodium) injection combined with chemotherapy for gastric cancer patients. Method: A consecutive cohort of 70 patients were divided into two groups: experimental group with cantharidin sodium injection combined with chemotherapy, while the control group received chemotherapy alone. After more than two courses of treatment, efficacy, quality of life and side effects were evaluated. Results: The response rate of experimental group was not significantly different from that of the control group (P>0.05), but differences were significant in clinical benefit response and KPS score. In addition, gastrointestinal reactions and the incidence of leukopenia were lower than in the control group (P<0.05). Conclusions: $Qinin^{(R)}$ (Cantharidin sodium) injection combined with chemotherapy enhances clinical benefit response, improving quality of life of gastric cancer patients and reducing side effects of chemotherapy. Thus $Qinin^{(R)}$ (Cantharidin sodium) injection deserves to be further investigated in randomized control clinical trails.

Clinical Safety and Efficacy of Kanglaite^® (Coix Seed Oil) Injection Combined with Chemotherapy in Treating Patients with Gastric Cancer

Zhan, Yi-Ping,Huang, Xin-En,Cao, Jie,Lu, Yan-Yan,Wu, Xue-Yan,Liu, Jin,Xu, Xia,Xiang, Jin,Ye, Li-Hong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10

Objective: To observe efficacy and side effects, as well as the impact on quality of life, of Kanglaite$^{(R)}$ (Coix Seed Oil) injections combined with chemotherapy in the treatment of advanced gastric cancer patients. Method: A consecutive cohort of 60 patients were divided into two groups: the experimental group receiving Kanglaite$^{(R)}$ Injection combined with chemotherapy and the control group with chemotherapy alone. After more than two courses of treatment, efficacy, quality of life and side effects were evaluated. Results: The response rate and KPS score of experimental group were significantly improved as compared with those of the control group (P<0.05). In addition, gastrointestinal reactions and bone marrow suppression were significantly lower than in the control group (P<0.05). Conclusions: Kanglaite$^{(R)}$ Injection enhanced efficacy and reduced the side effects of chemotherapy, improving quality of life of gastric cancer patients; use of Kanglaite$^{(R)}$ injections deserves to be further investigated in randomized control clinical trails.

Enzymatic Synthesis of Theanine with Escherichia coli γ-glutamyltranspeptidase from a Series of γ-glutamyl Anilide Substrate Analogues

Hong-juan Zhang,Wei-guo Zhang,Zhi-yuan Wang,Yue-ping Zhan,Li-sheng Xu,Jun-zhong Liu,Qian Liu,Qing-cai Jiao 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.2

In order to investigate the catalytic mechanism of Escherichia coli γ-glutamyltranspeptidase, ten para- and meta- substituted γ-glutamyl anilides were chemically prepared and employed as substrates to synthesize L-theanine to assay the activity of γ-glutamyltranspeptidase. The reaction was optimized for γ-glutamyl-p-nitroanilide. Key factors such as substrate specificity, pH, temperature, and the substrate mole ratio were all investigated. Kinetic studies of the acyl transfer reaction were described and the Hammett plot was constructed. This study indicated that the ratelimiting acylation reaction of γ-glutamyltranspeptidase can apparently be accelerated by either the electron-withdrawing or electron-donating substituents of γ-glutamyl anilides. The reaction could be catalyzed by the general acid and carboxy of Asp-433 or phenolic hydroxyl Tyr-444 may be the acid by autodock simulation for all prepared γ-glutamyl anilides.

Gentiopicroside Ameliorates the Progression from Hepatic Steatosis to Fibrosis Induced by Chronic Alcohol Intake

( Hong-xu Yang ),( Yue Shang ),( Quan Jin ),( Yan-ling Wu ),( Jian Liu ),( Chun-ying Qiao ),( Zi-ying Zhan ),( Huan Ye ),( Ji-xing Nan ),( Li-hua Lian ) 한국응용약물학회 2020 Biomolecules & Therapeutics(구 응용약물학회지) Vol.28 No.4

In current study, we aimed to investigate whether the gentiopicroside (GPS) derived from Gentiana manshurica Kitagawa could block the progression of alcoholic hepatic steatosis to fibrosis induced by chronic ethanol intake. C57BL/6 mice were fed an ethanol- containing Lieber-DeCarli diet for 4 weeks. LX-2 human hepatic stellate cells were treated with GPS 1 h prior to transforming growth factor-β (TGF-β) stimulation, and murine hepatocyte AML12 cells were pretreated by GPS 1 h prior to ethanol treatment. GPS inhibited the expression of type I collagen (collagen I), α-smooth muscle actin (α-SMA) and tissue inhibitor of metal protease 1 in ethanol-fed mouse livers with mild fibrosis. In addition, the imbalanced lipid metabolism induced by chronic ethanol-feeding was ameliorated by GPS pretreatment, characterized by the modulation of lipid accumulation. Consistently, GPS inhibited the expression of collagen I and α-SMA in LX-2 cells stimulated by TGF-β. Inhibition of lipid synthesis and promotion of oxidation by GPS were also confirmed in ethanol-treated AML12 cells. GPS could prevent hepatic steatosis advancing to the inception of a mild fibrosis caused by chronic alcohol exposure, suggesting GPS might be a promising therapy for targeting the early stage of alcoholic liver disease.