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- 저자 : Lending, Craig R. (검색결과 52 건)
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A Drosophila Phospholipase C Gene That Is Expressed in the Central Nervous System
Shortridge, Randall D.,Yoon, Jaeseung,Lending, Craig R.,Bloomquist, Brian T.,Perdew, Marcia H.,Pak, William L. 경희대학교 유전공학연구소 1991 遺傳工學論文集 Vol.3 No.-
A Drosophila phospholipase C (PLC) gene, designated as plc-21, was isolated by screening a genomic DNA library using a cDNA for a previously isolated Drosophila PLC gene, norpA, as probe under reduced stringency hybridization conditions. The gene maps to 21C on the left arm of the second chromosome. Two proteins of 1305 and 1312 amino acids, respectively, were deduced from two classes of cDNA which were isolated. The two putative plc-21 proteins are similar in sequence and overall structure to the β-class of PLCs found in mammals and differ from each other only by 7 amino acid residues that are present near the C terminus of one of the proteins but not the other. Hybridization of plc-21 cDNA probes to blots of poly(A)^(+) RNA revealed that the gene encodes a 7.0-kilobase transcript that could be detected in the head but not in the body of adult flies and a 5.6-kilobase transcript that could be detected throughout development and in both heads and bodies of adults. I n situ hybridization of cDNA sequences to tissue sections showed that the gene is expressed in the neuronal cell bodies of the optic lobe, central brain, and thoracic ganglia of adults and the brain of larvae. This tissue distribution of plc-21 transcripts is identical to the distribution of transcripts from a Drosophila Go a-subunit gene that we reported previously.
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Lent, Heather,Lee, Kyung-Eun,Park, Hyun-Seok Korea Genome Organization 2015 Genomics & informatics Vol.13 No.4
Recently, the Encyclopedia of DNA Elements (ENCODE) Analysis Working Group converted data from ChIP-seq analyses from the Broad Histone track into 15 corresponding chromatic maps that label sequences with different kinds of histone modifications in promoter regions. Here, we publish a frequency profile of the three ChromHMM promoter states, at 200-bp intervals, with particular reference to the existence of sequence patterns of promoter elements, GC-richness, and transcription starting sites. Through detailed and diligent analysis of promoter regions, researchers will be able to uncover new and significant information about transcription initiation and gene function.
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The gap between expectations and reality: integrating computers into mathematics classrooms
Bu¨ lent Gu¨ven 서울대학교 교육연구소 2009 Asia Pacific Education Review Vol.10 No.4
As a result of dramatic changes in mathematics education around the world, in Turkey both elementary and secondary school mathematics curriculums have changed in the light of new demands since 2005. In order to perform the expected change in newly developed curriculum, computer should be integrated into learning and teaching process. Teachers' beliefs play a key role in this integration process. Negative beliefs against using computer in mathematics teaching may lead to failure of this process. With the help of this study, it is aimed to detect mathematics teachers' beliefs concerning Computer Assisted Mathematics Instruction (CAMI). Within the scope of this aim, the conducted questionnaire (The opinions of teachers about using computer in Mathematics Instruction) has been carried out on 91 mathematics teachers in the city of Trabzon. The acquired results have shown that mathematics teachers have developed negative opinions against CAMI. This state has revealed that there is a huge inconsistency between curriculum's positive expectations arising from computer usage and teachers' convictions.
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Heather Lent,이경은,박현석 한국유전체학회 2015 Genomics & informatics Vol.13 No.4
Recently, the Encyclopedia of DNA Elements (ENCODE) Analysis Working Group converted data from ChIP-seq analyses from the Broad Histone track into 15corresponding chromatic maps that label sequences with different kinds of histone modifications in promoter regions. Here, we publish a frequency profile of the three ChromHMM promoter states, at 200-bp intervals, with particular reference to the existence of sequence patterns of promoter elements, GC-richness, and transcription starting sites. Through detailed and diligent analysis of promoter regions, researchers will be able to uncover new and significant information about transcription initiation and gene function.
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B\"{u}lent Nafi \"{O}rnek 대한수학회 2016 대한수학회보 Vol.53 No.2
In this paper, a boundary version of the Schwarz lemma is investigated. We take into consideration a function $f(z)=z+c_{p+1}z^{p+1}+c_{p+2}z^{p+2}+\cdots$ holomorphic in the unit disc and $\left\vert \frac{f(z)}{\lambda f(z)+(1-\lambda )z}-\alpha \right\vert <\alpha $ for $\left\vert z\right\vert <1$, where $\frac{1}{2}<\alpha \leq \frac{1}{1+\lambda }$, $ 0\leq $ $\lambda <1$. If we know the second and the third coefficient in the expansion of the function $f(z)=z+c_{p+1}z^{p+1}+c_{p+2}z^{p+2}+\cdots$, then we can obtain more general results on the angular derivatives of certain holomorphic function on the unit disc at boundary by taking into account $ c_{p+1}$, $c_{p+2}$ and zeros of $f(z)-z$. We obtain a sharp lower bound of $ \left\vert f^{\prime }(b)\right\vert $ at the point $b$, where $\left\vert b\right\vert =1$.
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Salas, R.C.D.,van der Lende, T.,Udo, H.M.J.,Mamuad, F.V.,Garillo, E.P.,Cruz, L.C. Asian Australasian Association of Animal Productio 2000 Animal Bioscience Vol.13 No.5
Data collected between 1981 and 1991 at the Philippine Carabao Center at Central Luzon State University (PCC-CLSU) were used for the comparison of growth, milk yield and draughtability of Murrah-Philippine crossbred and Philippine native buffaloes. Body weights and body measurements were available at 3-month intervals from birth to 36 months of age for a total of 34 $Murrah{\times}Philippine$ native buffalo F1 crossbreds (CBB; 21 cows, 13 bulls) and 32 Philippine native buffaloes (PNB; 16 cows, 16 bulls). Lactation records were available for 14 CBB and 19 PNB cows. Data for draughtability under wet and dry ploughing conditions were available for 4 CBB and 4 PNB steers. The results indicate that crossbreds grow faster (0-9 months of age: cows $442{\pm}19$ vs. $301{\pm}21g/day$, bulls $305{\pm}23$ vs. $296{\pm}21g/day$; 9-36 months of age: cows $227{\pm}10$ vs. $147{\pm}12g/day$, bulls $282{\pm}13$ vs. $138{\pm}12g/day$), mature earlier and produce more milk (1st lactation: $1139{\pm}153$ vs. $450{\pm}112kg$; 2nd lactation: $1115{\pm}132$ vs. $488{\pm}136kg$) than native buffaloes, but have a poorer draughtability (wet ploughing; force as % of body weight $8.8{\pm}0.2$ vs. $12.2{\pm}0.6$; dry ploughing: cut depth $10.98{\pm}0.25$ vs. $11.92{\pm}0.13cm$, velocity $0.50{\pm}0.03$ vs. $0.60{\pm}0.02m/sec$, force as % of body weight $9.0{\pm}0.6$ vs. $11.3{\pm}0.7$). The correlation coefficients between body weight and body measurements at birth and at 3-month intervals indicate that heart girth has a relatively high correlation with body weight, especially in crossbreds. It is concluded that in Philippine smallholder farming systems in which meat and milk production are secondary to draught power, the native buffalo is preferable from the point of view of input needed to maintain the number of animals kept for a required draught force.
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Antipneumococcal Activity of DW-224a, a New Quinolone, Compared to Those of Eight Other Agents
Kosowska-Shick, Klaudia,Credito, Kim,Pankuch, Glenn A.,Lin, Gengrong,Bozdogan, Bü,lent,McGhee, Pamela,Dewasse, Bonifacio,Choi, Dong-Rack,Ryu, Jei Man,Appelbaum, Peter C. American Society for Microbiology 2006 Antimicrobial agents and chemotherapy Vol.50 No.6
<B>ABSTRACT</B><P>DW-224a is a new broad-spectrum quinolone with excellent antipneumococcal activity. Agar dilution MIC was used to test the activity of DW-224a compared to those of penicillin, ciprofloxacin, levofloxacin, gatifloxacin, moxifloxacin, gemifloxacin, amoxicillin-clavulanate, cefuroxime, and azithromycin against 353 quinolone-susceptible pneumococci. The MICs of 29 quinolone-resistant pneumococci with defined quinolone resistance mechanisms against seven quinolones and an efflux mechanism were also tested. DW-224a was the most potent quinolone against quinolone-susceptible pneumococci (MIC50, 0.016 μg/ml; MIC90, 0.03 μg/ml), followed by gemifloxacin, moxifloxacin, gatifloxacin, levofloxacin, and ciprofloxacin. β-Lactam MICs rose with those of penicillin G, and azithromycin resistance was seen mainly in strains with raised penicillin G MICs. Against the 29 quinolone-resistant strains, DW-224a had the lowest MICs (0.06 to 1 μg/ml) compared to those of gemifloxacin, clinafloxacin, moxifloxacin, gatifloxacin, levofloxacin, and ciprofloxacin. DW-224a at 2× MIC was bactericidal after 24 h against eight of nine strains tested. Other quinolones gave similar kill kinetics relative to higher MICs. Serial passages of nine strains in the presence of sub-MIC concentrations of DW-224a, moxifloxacin, levofloxacin, ciprofloxacin, gatifloxacin, gemifloxacin, amoxicillin-clavulanate, cefuroxime, and azithromycin were performed. DW-224a yielded resistant clones similar to moxifloxacin and gemifloxacin but also yielded lower MICs. Azithromycin selected resistant clones in three of the five parents tested. Amoxicillin-clavulanate and cefuroxime did not yield resistant clones after 50 days.</P>
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Cho, Soo-Jeong,Yoon, Changhwan,Lee, Jun Ho,Chang, Kevin K.,Lin, Jian-xian,Kim, Young-Ho,Kook, Myeong-Cherl,Aksoy, Bu¨lent Arman,Park, Do Joong,Ashktorab, Hassan,Smoot, Duane T.,Schultz, Nikolaus,Yoon, American Association for Cancer Research 2018 Clinical Cancer Research Vol.24 No.24
<P><B>Purpose:</B></P><P>Lauren diffuse-type gastric adenocarcinomas (DGAs) are generally genomically stable. We identified lysine (K)-specific methyltransferase 2C (<I>KMT2C</I>) as a frequently mutated gene and examined its role in DGA progression.</P><P><B>Experimental Design:</B></P><P>We performed whole exome sequencing on tumor samples of 27 patients with DGA who underwent gastrectomy. Lysine (K)-specific methyltransferase 2C (<I>KMT2C</I>) was analyzed in DGA cell lines and in patient tumors.</P><P><B>Results:</B></P><P><I>KMT2C</I> was the most frequently mutated gene (11 of 27 tumors [41%]). KMT2C expression by immunohistochemistry in tumors from 135 patients with DGA undergoing gastrectomy inversely correlated with more advanced tumor stage (<I>P</I> = 0.023) and worse overall survival (<I>P</I> = 0.017). KMT2C shRNA knockdown in non-transformed HFE-145 gastric epithelial cells promoted epithelial-to-mesenchymal transition (EMT) as demonstrated by increased expression of EMT-related proteins N-cadherin and Slug. Migration and invasion in gastric epithelial cells following KMT2C knockdown increased by 47- to 88-fold. In the DGA cell lines MKN-45 and SNU-668, which have lost KMT2C expression, KMT2C re-expression decreased expression of EMT-related proteins, reduced cell migration by 52% to 60%, and reduced cell invasion by 50% to 74%. Flank xenografts derived from KMT2C-expressing DGA organoids, compared with wild-type organoids, grew more slowly and lost their infiltrative leading edge. EMT can lead to the acquisition of cancer stem cell (CSC) phenotypes. KMT2C re-expression in DGA cell lines reduced spheroid formation by 77% to 78% and reversed CSC resistance to chemotherapy via promotion of DNA damage and apoptosis.</P><P><B>Conclusions:</B></P><P><I>KMT2C</I> is frequently mutated in certain populations with DGA. KMT2C loss in DGA promotes EMT and is associated with worse overall survival.</P>
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