부신피질 호산성 과립세포종 1예

이성진,이호권,박철영,정인경,홍은경,오기원,김현규,김두만,유재명,임성희,최문기,유형준,박성우 대한내분비학회 2004 Endocrinology and metabolism Vol.19 No.1

저자들은 건강검진에서 시행한 복부 초음파검사상 우연히 좌측 부신 종괴가 발견되어 복부 전산화 단층 촬영검사와 호르몬검사를 시행한 후 부신피질 악성종양과의 감별 진단을 위해 부신절제술과 전자현미경검사를 포함한 병리조직학적 검사를 시행하여 부신피질호산성 과립세포종으로 진단한 증례를 경험하였기에 문헌고찰과 함께 이를 보고하는 바이다. Oncocytomas are neoplasms, histologically are composed of epithelial cells, with abundant, acidophilic and granular cytoplasm. Electron microscopic studies of oncocytomas have shown that the cytoplasm of oncocytes is packed with mitochondria. The adrenal gland is a very rare anatomical site for oncocytomas, and to the best of our knowledge, only thirty-six cases of adrenal oncocytomas have been described. Herein, a case of a large adrenal mass in a forty-year-old man, which was incidentally detected by abdominal ultrasonography, is presented. This patient demonstrated no clinical manifestation associated with adrenal hyperfunction. Hormonal studies showed no abnormal findings, except for a mild elevation of the 24-hour urinary VMA level. Abdominal computed tomography with enhancement revealed a large, well-defined left adrenal mass, measuring 5.0×4.2 ×3.0 cm. The patient underwent a left adrenalectomy, and a light microscopic examination confirmed an adrenocortical oncocytoma, with characteristic oncocytes and polygonal, abundant, eosinophilic and granular cytoplasm. The tumor cells were positive for cytokeratin and vimentin as well as S-100, but negative for chromogranin on immunohistochemical staining. An electron microscopic examination demonstrated closely packed mitochondria, containing intramitochondrial inclusions. After surgery, there was no evidence of a recurrent or distant metastatic disease at the 5 month follow-up. In summary, an extremely rare case of a man with an adrenocortical oncocytoma is reported, which was confirmed by histological examinations, including electron microscopy (J Kor Soc Endocrinol 19:82∼89, 2004).

횡성댐 水沒地域內의 植生에 關한 硏究 : 移植對象區域을 中心으로 Centered with Subdivided Area to Select Plants Capable of Transfer

이우철,이기의,서옥하,조현길,백원기,김경진 江原大學校 森林科學硏究所 1997 Journal of Forest Science Vol.13 No.-

본 조사 연구는 江原道 橫成郡 甲川面과 橫成邑 일부를 포함하는 지역에 횡성댐이 건설됨에 따라 수몰되어질 自然生態系의 復元과 保存計劃의 일부로 수행되어졌다. 移植對象區域인 7개소를 중심으로 綠地自然度, 植生現存量 및 생산량 種組成과 植生에 관해 조사하였으며 그 결과는 다음과 같다. 1. 綠地自然度를 등급별로 사정한 결과 등급 0(3.01%), 1(5.3%), 2(28.2%), 3(0.1%), 5(0.2%), 7(4.6%), 7(44.7%), 및 8(13.8%)의 8개 등급으로 구분되었다. 2. 전체조사지역의 現存量과 生産量은 각각 88,436.3 ton/year 과 12,960.3 ton/year 이었다. 3. 조사지역의 陸上植物의 種組成은 86科, 221屬, 306種, 1變種, 3品種의 총 310種으로 조사되었다. 4. 선정된 7개의 植生調査地點에서는 소나무군락이 憂占하며, 그밖에 신갈나무군락, 떡갈나무군락, 상수리나무군락으로 나타났다. This study was carried out in a part of conservative plans recovering natural ecosystem which will be submerged, as Hoengseong dam is being constructed around Hoengseong-Eup and Gabcheon-Myun. The submerged area was divided into 7 small areas to figure out what kinds of species of plants are and will be according to detailed geographic characteristics. In the center of the subdivided areas. Degree of Green Naturality, standing biomass, net 36production per year, and composition of plant species were investigated. The results are as following; 1. The surveyed area was classified into 8 degree by Degree of Green Naturality, and the appeared degree and the rate of occupation were 0(3.01%), 1(5.3%), 2(28.2%), 3(0.1%), 5(0.2%), 7(4.6%), 7(44.7%), 및 8(13.8%). 2. Standing biomass was 88,436.3 ton/year and net production per year was 12,960.3 ton/year. 3. The list of vascular plants identified in this study consisted of 86 families, 221genera, 1 variety, 3 formae and 306 species, totally summiting upto 310 species. 4. The 7 surveyed areas were largely occupied by Pinus community. In addition, there were Quercus mongolica, Quercus dentata, and Quercus acutissima communities.

한 대학병원 정신과 낮병원에 관한 분석적 고찰

정원철,이영호 慶南專門大學 1999 論文集 Vol.27 No.1

간척지 중염답에서 유수형성기 염처리에 따른 벼 생육 및 수량 변화

최원영,이규성,고종철,김상수,김태수 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.3

본 연구는 새계화벼를 공시하여 간척지 중염답(0.3~0.4%)에서 관개수 염농도에 따른 생육 및 수량성을 검토하고자 유수형성기에 5일간 염수를 처리하여 시험한 결과를 요약하면 다음과 같다. 1. 관개수 염분농도가 높을수록 벼 출수는 1~2일 지연되었고, 출수기 지상부건물중은 가벼워졌다. 2. 간장과 수장은 관개수 염분농도가 높을수록 짧아졌다. 3. 관개수 염분농도가 높을수록 수당 립수가 적어 ㎡당 립수가 적었으며, 등숙비율이 낮고 현미 천립중이 가벼워, 쌀 수량은 민물 관개(무처리)의 330㎏/10a에 비해 관개수 염분농도 0.1%는 94%, 0.3%는 85%, 0.5%는 76%, 0.7%는 71%의 수량을 얻었다. 4. 관개수 염분농도가 높을수록 완전립 비율은 낮아졌다. 따라서 간척지에서 중염도 토양(0.3~0.4%)에서 벼를 재배하여 유수형성기에 한발이 닥쳐 저류지 등 염분 농도 0.7%의 물로 5일간 담수하여도 쌀 수량은 29% 정도 감수되고 쌀의 품위도 저하되지만 어느 정도 수량은 얻을 수 있을 것으로 생각된다. This experiment was conducted at the Kyehwado Substation of the National Honam Agricultural Experiment Station(NHAES) RDA, Korea. The experimental field contained 0.38% NaCl in soil solution. The experiment was involved five treatments (control, 0.1, 0.3, 0.5, 0.7%) in a randomized complete block design with three replicates, saline solution was applied only one time at panicle formation stage for 5 days. Saegyehwabyeo, a japonica rice variety was used in this experiment. In yield components, Spikelets number per ㎡ decreased with increasing salinity level, particularly in the 0.7% of saline solution water. This factor affected the most yield reduction among the components. The percentage of ripened grain was inclined to decrease with increasing salinity level. 1,000 grain weight decreased with increasing salinity level but it was lest affected by salinity among yield components. The reduction of milled rice yield decreased significantly with increased saline water level, in detail 6% of yield reduction at the 0.1% saline solution, 15% at the 0.3%, 24% at the 0.5%, and 29% at the 0.7% saline solution level compared with control respectively. Results indicate that the gaining of rice yield could be reliable performance in terms of economical benefit for rice production even though high reduction of yield occurred at high salinity levels on a reclaimed saline soil in Korea.

다극형 스파크 �b과 다중평행판 축전기형 전송회로를 사용한 질소레이저의 동작특성

장철용,박영수,양준목,이치원 충남대학교 기초과학연구소 1994 忠南科學硏究誌 Vol.21 No.1

We have studied on the operational characteristics of a nitrogen laser which was coupled with a multiplex parallel capacitor type Blumlein transmission line circuit and a multiplex sparkgap. This spark gap consisted of 8 pairs of electrode and was 50 ㎝ in length, as long as the laser tube. According to increasing the number of electrodes in the spark gaps up to the 6 pairs, the output power and stability of the laser was increased. It was to be improved that the multiplex spark gap was operated as a good peformance to the nitrogen laser system. The optimum operational condition of the nitrogen laser at 20㎐ of repetition rate could be obtained with the nitrogen gas pressure of 80 torr, E/P value of 325.0 v/㎝ torr at the electrodes spacing 5.0 ㎜ and of 90 torr, E/P value of 311.1 v/㎝ torr at the electrodes spacing 6.0 ㎜ respectively.

평야지재배 조생종 벼의 이화학적 및 식미특성 분석

정진일,김기영,최윤희,최원영,고종철,오명규,홍하철,이승엽,이명철 한국국제농업개발학회 2004 韓國國際農業開發學會誌 Vol.16 No.4

평야지재배 적응 조생종 벼 품종의 선발 및 품종 육성의 기초 자료로 활용코자 극조생종(1품종)과 조생종(10품종) 및 준조생(3품종)등 총 14품종을 산간지(운봉)와 평야지(익산)에 지역별 표준재배법으로 공시한 결과, 1. 극조생종 및 조생종들은 감온성이 커 산간지에서 출수가 빠르나, 준조생종에 속하는 상미벼, 영덕34호, 히또메보레 등은 감광성이 감온성보다 커, 평야지재배에서 4일정도 빨리 출수하였다. 2. 수량성은 대부분의 품종들이 산간지재배에 비해 평야지 재배에서 높았고 대체로 출수가 빠른 품종일수록 지역간의 수량차가 크게 나타났으며, 출수가 다소 늦은 준조생종들은 산간지와 평야지의 재배에서 수량차가 크지 않아 산간지 뿐만 아니라 평야지에서도 적응성이 높았다. 3. 단백질함량은 숙기가 다소 늦은 품종일수록 낮은 경향을 보였고 산간지보다는 평야지에서 낮았으며, 품종간에는 오대벼, 운봉30호, 영덕34호, 신석조생, 상미벼 등이 함유량이 낮았다. 4. 식미치는 산간지재배가 평야지에 비해 보다 높게 나타났고, 출수가 늦을수록 높았으며, 지역간 차가 크게 나타난 품종은 극조생종인 키라라397이었고, 출수가 늦은 상미벼와 히또메보레는 차가 적었다. 5. 산온도와 수량과의 상관은 산간지에서만 영향이 있을 뿐 평야에서는 영향이 적고 일사량과 일조시수가 수량에 보다 상관성이 높았으며, 식미치는 적산온도와는 부의상관, 생육 일수와는 정상관을 보였다. This experiment was carried out to clarify the difference of rice yield and the quality of rice in southern plan area and in alpine area. Yield and quality of fourteen early varieties were investigated under the different transplanting dates and planting densities in southern plan area(Iksan) and in alpine area(Unbong). In the alpine area, extremely early variety and early varieties headed early compared to the plain area, but medium early varieties headed about 4 days late unlike the alpine area. The yield difference of most early varieties including Kilara 397 of extremely early flowering variety were greater in plain area than in alpine. The different of yield between plain and alpine area was less in varieties with Sinseogjosaeng, Odaebyeo, Akidagomachi, and Yeungdeuk34. The rate of protein was lower in plain area than alpine and more lately flowering than early, but palatability of milled rice was higher at the alpine area and more lately flowering varieties. Correlation coefficients between yield and meterological data during maturity, yield shower the significant correlation with integrated solar radiation and sunshine hours at cultured in southern plan area, but it showed only cumulative temperature in alpine area. Palatability of miller rice was positive correlation at growth duration but negative correlation at cumulative temperature.

8-hydroxyguanine이 전립선비대증 발생에 미치는 효과

이재원,이석영,이형래,이상철,김용태,김원재 충북대학교 의학연구소 2002 忠北醫大學術誌 Vol.12 No.2

연구목적: 전립선비대증은 노화현상에 의해 발생하는 질환으로서 임상적으로 주로 50세 이상에서 증상이 나타난다. 산소 라디칼은 DNA의 염기에 다양한 손상을 미칠 수 있다. 산소 라디칼에 의한 DNA의 변화에는 여러 가지 형태가 있으며 이러한 변화들 가운데서 guanine이 8-oxoguanine (8-hydroxyguanine, oh8Gua)으로의 수산화(hydroxylation) 반응은 가장 흔히 관찰되는 형태로서 노화 및 만성 퇴행성 질환, 기타 여러 질환의 중요한 병인으로 인식되고 있다. 세포에 유해한 영향을 주는 oh8Gua의 생성으로부터 유전자를 보호하는 수복효소 중 8-oxoguanine-DNA glycosylase (OGG1)가 주된 역할을 하는 것으로 알려져 있는데 이 효소를 생산하는 유전자인 hOGG1은 사람마다 유전자형이 다르다. 따라서 본 연구에서는 전립선비대증이 노화와 관련된 hOGG1 유전자형에 따라서 발생에 차이가 있는지를 조사하였다. 대상 및 방법: 충북대학교 병원에서 전립선비대증으로 진단 받고 입원하여 경요도전립선절제술을 받은 환자로 병리 조직학적으로 전립선비대증이 확인된 81명을 환자군으로 하였으며, 충청북도내 10개 군에서 50세 이상의 건강한 성인 남자 764명을 대상으로 실시한 전립선 무료검진에서 경직장 초음파 검사상 전립선 크기 20 gm 이하, 국제 전립선 증상 점수(IPSS) 7점 이하, 요속 10 ㎖/s 이상, 전립선 특이 항원(PSA) 4.0 ng/㎖ 이하인 81명을 대조군으로 설정하였다. 이들에서 채취한 혈액에서 genomic DNA를 얻은 후 PCR-SSCP (polymerase chain reaction-single stranded conformation polymorphism), 직접 염기서열분석(direct DNA sequencing) 및 RFLP (restriction fragment length polymorphism)를 통해서 두 군 사이의 hOGG1 유전자의 다형성을 분석하였다. 결과: 대조군에서 codon 326형은 Ser326Ser형이 12례(14.8%), Ser326Cys형이 51례(63.0%), Cys326Cys형이 18례(22.2%)였으며 전립선비대증 환자군에서는 Ser326Ser형이 15례(18.5%), Ser326Cys형이 34례(42.0%), Cys326Cys형이 32례(39.5%)로 두 군 사이에 통계적인 유의성이 관찰되었다(p=0.022). 또한 Cys326Cys형과 나머지(Ser326Ser, Ser326Cys)형으로 나누어 시행한 통계 분석에서도 대조군에서는 각각 18례(22.2%), 63례(77.8%)였으며 전립선비대증 환자군에서는 32례(39.5%), 49례(60.5%)로 통계적인 유의성이 관찰되었다(p=0.017, OR=2.286, 95% CI=1.149-4.546). 결론: hOGG1 유전자의 형태와 전립선비대증의 발생과는 밀접한 관련이 있었다. 즉 hOGG1 Cys326Cys형을 가진 사람에서 전립선비대증이 발생한 가능성이 높을 것으로 사료된다. Purpose: 8-oxoguanine DNA glycosylase (OSS1) rapairs DNA by removing 8-oxoguanine (oh8Gua), a highly mutagenic oxidative DNA adduct. Recently, the human gene for OGG1 (hOGG1) was cloned and several genotypes have been reported. However, the implications of such genotypes in benign prostatic lyperplasia have not been demonstrated. To assess the involvement of hOGG1 associated with the aging process on the developing of BPH, we analyzed the genetic polymorphisms of hOGG1. Materials and Methods: We studied the hOGG1 gene polymorphisms in 81 cases of BPH and 81 cases of normal controls. We performed the SSCP, PCR-based restriction fragment length polymorphism (RFLP) and direct sequencing to characterize the genetic polymorphisms of hOGG1 in both BPH patients and controls. Results: We found two polymorphic sites. A serine326/cysteine polymorphism at codon 326 (1a type) in exon 7 was associated with an exchange of amino acid. Another polymorphic site at codon 324 (1b type) in exon 6 was silent. We found that the distribution of hOGG1 genotypes in BPH patients (Ser326Ser type 18.5%, Ser326Cys type 42.0% and Cys326Cys type 39.5%) was significantly different from that in controls (14.8%, 63.0% and 22.2%, respectively) (p=0.022). Homozygosity for the Cys326Cys genotype significantly increased the risk of developing BPH, with the odds ratio (OR) being 2.286 (95% confidence interval [CI]=1.149-4.546) Conclusion: Our results suggest that the hOGG1 Cys326Cys genotype might play a important role in the development of BPH.

Employment of Hordein Subunit Polymorphisms in Establishing Selection Criteria for High Quality Malting Barley (Hordeum vulgare L.)

Lee, Yong-Jin,Lee, Tong-Geon,Jeon, Woong-Bae,Kim, Dae-Yeon,Hong, Min-Jeong,Lee, Man-Bo,Hyun, Jong-Nae,Kim, Mi-Jung,Lee, Mi-Ja,Park, Cheol-Su,Seo, Yong-Weon 한국작물학회 2010 Journal of crop science and biotechnology Vol.13 No.2

Cereal seed storage proteins are encoded by complex multigene families and their subunit profiles are highly related to end-use qualities. Each fraction of albumin and hordein was extracted and its subunit profile was evaluated in related to malt and grain quality parameters. The purpose of this study was to provide selection criteria for high quality malting barley using grain and malt quality parameters and biochemical-genetic information. Grain and malt quality of 13 local adaptability test (LAT) lines were evaluated for malting process. A total of 16 germplasm accessions of high or low seed storage protein content were also evaluated for biochemical-genetic analysis. The correlation coefficients among quality parameters were analyzed. Several important quality parameters in brewing process showed significant positive or negative correlations. Seed storage protein subunits of albumin and hordein of all tested lines and accessions were evaluated using 12% 1D SDS-PAGE. Scored data of protein subunit's presence or absence was applied to Agglomerative Hierarchical Clustering (AHC) for statistical analysis and showed specific grouping patterns among tested lines. Clustered lines with subunit information were highly related with agricultural performance and grain and malt qualities. Based on the profiles of seed storage protein subunits, association of hordein subunit of 38, 43, and 65 kDa with high malt scored lines was found. The obtained results would provide improved selection criteria for high quality malting barley in the malting barley breeding program.

Digital Modulation Classification for MPSK Signal by Using the Transformation of Random Variables

Weon Cheol Lee,Young Yearl Han 대한전자공학회 1993 International Conference on Electronics, Informati Vol.2 No.1

Classification of Celiac disease epitopes of ω-gliadin through data mining and compared with Chinese spring genome sequence

Cheol Won Lee,Yong Weon Seo 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Celiac disease (CD) is classified as an autoimmune disease of small intestine and occurred with people with the human leucocyte antigen (HLA) DQ2(8) cells. The gluten commonly called for the gliadins and glutenins from wheat and related proteins from barley and rye is significant cause of celiac disease. There are many sequences that recognized by T-cell according to species and different types of gliadins. In ω-gliadin, two sort of epitopes were figured out that consisting of some proline(P) and glutamine(Q) scattered in gliadin sequence. All registered ω-gliadin sequences deposited in NCBI database were downloaded and collected. In order to classify groups depending on sequence difference, sequence similarity and their closeness were analyzed by phylogenetic trees using by MEGA (ver.6.06). Chinese spring genome sequence database offered by URGI (Unité de Recherche Génomique Info) is used for sequence assembly. Primers to validate presence of epitopes were designed by two different type from conserved and specific region. Primer pair from consensus region were designed in conserved domain of ω-gliadin sequences from public database by sequence alignment. And, sequence-specific primers of ω-gliadin were designed from the unique region of each ω-gliadin sequence comparing ω-gliadin sequences from NCBI database with draft sequence of Chinese spring in URGI. The two known epitopes of ω-gliadin were located on same site, approximately from the 315th nucleotide to the 348th nucleotide in CDS. Candidate epitopes present in ω-gliadin were divided into three categories based on analysis of sequence similarity. This categorization shows similar pattern with groups that were previously reported by sequence motifs such as SRLL, AREL, ARQL and KELQ. However, sequence which has AREL motif and sequence ARQL motif were not distinguished obviously in ω-gliadin based on sequence alignment.