크롬(Ⅵ)의 체내 흡수와 소실속도에 관한 연구

김현영,이성배,임철홍,이권섭,정용현,이종성,한정희,전윤석,황호순,이용묵 한국산업위생학회 2003 한국산업보건학회지 Vol.13 No.1

The CrO3 mostly used in plating. metal surface disposal, leather, cosmetic manufacturing, as an experiment material by repeatedly inhaling and exposure the male S.D. rats at a 0.00, 0.2., 0.50, 1.25 mg/㎡ concentration(particle size: 0.5-0.5 aerosol)6hours a day, 5day a week in 13weeks comparing with 2weeks, 8weeks of recovery group about the noxiousness of the experiment animal and the reduce scale of the CrO3 in the internal organ especially in blook and respiratory organ with the period of convalescent and clearance. The experiment results which we received are as follows. 1. In blood the RBC, HGB and HCT experiment, rats with 0.20, 0.50 mg/㎡ concentration showed that there were some decreases but not dependent. The kidneys absolute weight compared with control group was reduced intentionally(p〈0.05) and the lungs absolute weight compared with control group showed intentional increase(p〉0.05). 2. After the exposure of the experiment material, the whole blook, l\blood plasma and red blood cell in blood by (x): the period of convalescent, per (y); the decreasing of Cr concentration, was y=66.51 e -0.057x, y=67.2 e-0.101x, y=70.01 e-0.030 in 0.50 mg/㎡ exposure concentration by calculating the clearance coefficient of correlation, and the half life (day)was estimated 12.0, 6086, 23.0 each. 3, After the exposure of the experiment material, the experiment animals lung, liver and kidneys by(x); the period of convalescent, per (y); the decreasing of Cr conentration, was y=1808 e-0.00493x, y=12.02e-0.0297x, y=67.61 e-0.0292x in 0.50mg/㎡ exposure concentration by calculating the clearance coefficient of correlation, and the half life(day)was estimated 140.6, 23.3, 23.7, each, and including lung, liver with all of the experiment internal organs, the Cr clearance decreased as the exposure concentration increased.

SD Rats를 이용 1-hexene의 흡입독성 연구

김현영,임철홍,정용현,이권섭,이성배,이준연,한정희,전윤석,이용묵 한국환경독성학회 2001 환경독성보건학회지 Vol.16 No.4

The purpose of this study was to investigate the acute (4 hours) and repeated-dose (6 hours a day, 5 days a week, 4 weeks) toxic effects of I -hexene on Sprague- Dawley (SD) rats which were treated by inhalation. The results were as follows; I. The median lethal concentration (LC_(50)) was estimated 52,694 ppm (confidence limit 95%; 49,494~55,447 ppm) in acute inhalation. Abnormal clinical signs related to the l-Hexene were not observed with the acute inhalation dose. Gross findings of necropsy revealed on evidence of specific toxicity related to the 1-hexene, II. By repeated inhalation exposure the body weight of male were more or less reduced by the dose of 2,500 ppm and 5,000 ppm compared with control group. However there were no significant variation hematology and blood biochemistry for the exposed rats compared with the control rats. Abnormal clinical signs and gross findings of necropsy related to the 1-hexene were not shown. In conclusion when we exposed I-hexene to SD rats for 4 weeks, 5 days per week, 6 hours per day, the Lowest observed effect level (LOEL) was over 2,500 ppm and Non observed effect level (NOEL) was below 500 ppm.

원발성 유방암에서 p53 단백발현과 예후와의 상관성에 관한 연구

이학승,이광만,채권묵,문형배 圓光大學校 醫科學硏究所 1995 圓光醫科學 Vol.11 No.1

p53 is a nuclear phosphoprotein which is normally expressed at very low level in all mammalian cells and plays a role in the regulation of cell proliferation. It has also been suggested that normal p53(wild type) serves as a tumor suppressor gene, and inhibits the oncogene-mediated cellular transformation and the rate of cellular proliferation. But, wild type p53 is hardly stained by immunohistochemistry because of its low intracellular concentration and very short half-life. Mutant form of p53 protein is detectable in various human malignancies e.g. colon, stomach, lung and breast cancer by immunohistochemical stain because of its prolonged half-life. In breast cancer, p53 protein expression has been regarded as an unfavorable prognostic factor, but the results of studies about the relationship between the p53 protein expression and prognosis are equivocal. So, author performed this study to evaluate the prognostic significance of p53 expression in 54 patients with primary breast cancer who underwent surgical treatment at Wonkwang University Hospital from October 1985 to September 1991. Follow-up period was 24-91 months (mean: 54.7months). p53 protein was stained by immunohistochemical methods in formalin-fixed, paraffin-embedded tissues using monoclonal antibody(DAKO-p53, DO-7). The prognostic significance of p53 protein was evaluated by positivity and 5-year survival rate, and comparing with well-known prognostic factors of breast cancer. p53 protein was expressed 48.1%(26/54) of primary breast cancers. 5-year survival rate of patients with p53 protein expression was 42.0%, and that of patients without p53 protein expression was 76.4%(p = 0.0277) There was no relationship between p53 protein expression and tumor size, lymph node metastasis, histologic grade or vascular invasion. These results suggest that p53 protein expression is another independent prognostic factor in primary breast cancer.

축구 오버헤드 킥 동작의 운동학적 분석

김의환,이요열,김성섭,권문석,김성호 한국운동역학회 2003 한국운동역학회지 Vol.13 No.1

Kim, E-H · Lee , Y-Y · Kim, S-S· Kwon, M-S · Kim, S-H. The kinematical Analysis of the Overhead Kick on Soccer. Korean Journal of Sport Biomechanics. Vol. 13, No. 1,pp. 155-171. The purpose of this study was to analyze the kinematic variables of over head Kick(OHK) in soccer with three dimensional analysis technique and show the kinematic characteristics of it. The 7 subjects were university football player who have been playing football more than 7 years. The OHK was filmed on 16mm video camera(30frame/sec.) kinematic variables were temporal, postures, and COG(center of gravity). The mean values and the standard deviation for each variables were obtained and used as basic factors for examining characteristics of OHK. the results of this analysis were as follows : Temporal variables : The total time elapsed(TE) of OHK was0.94~1.14sec., the 1st phase was 0.35sec., 2nd phase was 0.46sec., and 3rd phase was 0.22sec.. Posture variables : When subjects performed OHK at the impact event, the ankle and knee angle of kicking foot were more extend than supporting foot. but the hip angle of supporting foot were more extend than kicking foot. Moving distance of the center of mass of the both foot : When subject performed OHK at the impact event, the range of distance on mediolateral direction aspect into right · left shoulder line, anteroposterior direction aspect was 20.9±10.5cm, vertical direction aspect was 92.3±19.9cm. Angular velocity : the faster angular velocity of knee · ankle on the kicking foot grew form jump position to landing position, the faster velocity of ball became. C. O. G. variables : When subject performed OHK at the impact event, upper part of the body was getting lower, lower part of the body was getting higher.

Dependence of Hot Carrier Reliability and Low Frequency Noise on Channel Stress in Nanoscale n-Channel Metal-Oxide-Semiconductor Field-Effect Transistors

Kwon, Hyuk-Min,Bok, Jung-Deuk,Han, In-Shik,Park, Sang-Uk,Jung, Yi-Jung,Jang, Jae-Hyung,Ko, Sung-Yong,Lee, Won-Mook,Lee, Ga-Won,Lee, Hi-Deok IOP Publishing 2011 Japanese journal of applied physics Vol.50 No.10

Plasma pharmacokinetics and metabolism of the antitumour drug candidate 2'-benzoyloxycinnamaldehyde in rats.

Lee, Kiho,Kwon, Byoung-Mog,Kim, Kangjeon,Ryu, Jekyung,Oh, Soo Jin,Lee, Kye Sook,Kwon, Mu-Gil,Park, Song-Kyu,Kang, Jong Soon,Lee, Chang Woo,Kim, Hwan Mook Taylor Francis 2009 Xenobiotica Vol.39 No.3

<P>The pharmacokinetics and metabolism of 2'-benzoyloxycinnamaldehyde (BCA) was characterized in male Sprague-Dawley rats as part of the preclinical evaluations for developing this compound as an antitumour agent. BCA was not detected in the plasma following either intravenous or oral dose, whereas its putative metabolites 2'-hydroxycinnamaldehyde (HCA) and o-coumaric acid were present at considerable levels. In separate pharmacokinetics studies, HCA exhibited a high systemic clearance and a large volume of distribution, whereas both pharmacokinetic parameters were much lower for o-coumaric acid. The terminal half-life of both metabolites was approximately 2 h. BCA was converted rapidly to HCA in rat serum, liver microsomes and cytosol in vitro; HCA was subsequently converted to o-coumaric acid in a quantitative manner only in the liver cytosol. In addition, the formation of o-coumaric acid was inhibited significantly by menadione, a specific inhibitor for aldehyde oxidase. Taken collectively, the results suggest that the rapid systemic clearance of HCA is likely due mainly to hepatic clearance occurring from aldehyde oxidase-catalysed biotransformation to o- coumaric acid. In conclusion, the present work demonstrates that the anticancer drug candidate BCA is highly likely to work as its active metabolite HCA in the body.</P>

Synthesis and Biological Evaluation of Manassantin Analogues for Hypoxia-Inducible Factor 1α Inhibition

Kwon, Do-Yeon,Lee, Hye Eun,Weitzel, Douglas H.,Park, Kyunghye,Lee, Sun Hee,Lee, Chen-Ting,Stephenson, Tesia N.,Park, Hyeri,Fitzgerald, Michael C.,Chi, Jen-Tsan,Mook Jr., Robert A.,Dewhirst, Mark W.,Le American Chemical Society 2015 Journal of medicinal chemistry Vol.58 No.19

<P/><P>To cope with hypoxia, tumor cells have developed a number of adaptive mechanisms mediated by hypoxia-inducible factor 1 (HIF-1) to promote angiogenesis and cell survival. Due to significant roles of HIF-1 in the initiation, progression, metastasis, and resistance to treatment of most solid tumors, a considerable amount of effort has been made to identify HIF-1 inhibitors for treatment of cancer. Isolated from <I>Saururus cernuus</I>, manassantins A (<B>1</B>) and B (<B>2</B>) are potent inhibitors of HIF-1 activity. To define the structural requirements of manassantins for HIF-1 inhibition, we prepared and evaluated a series of manassantin analogues. Our SAR studies examined key regions of manassantin’s structure in order to understand the impact of these regions on biological activity and to define modifications that can lead to improved performance and drug-like properties. Our efforts identified several manassantin analogues with reduced structural complexity as potential lead compounds for further development. Analogues <B>MA04</B>, <B>MA07</B>, and <B>MA11</B> down-regulated hypoxia-induced expression of the HIF-1α protein and reduced the levels of HIF-1 target genes, including cyclin-dependent kinase 6 (Cdk6) and vascular endothelial growth factor (VEGF). These findings provide an important framework to design potent and selective HIF-1α inhibitors, which is necessary to aid translation of manassantin-derived natural products to the clinic as novel therapeutics for cancers.</P>

Analgesic effectiveness of nerve block in shoulder arthroscopy: comparison between interscalene, suprascapular and axillary nerve blocks.

Lee, Sang Mook,Park, Sang-Eun,Nam, Yong-Seok,Han, Seung-Ho,Lee, Kwang-Jin,Kwon, Min-Jeong,Ji, Jong-Hun,Choi, Syung-Kyun,Park, Jang-Su Springer International 2012 KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY Vol.20 No.12

<P>Postoperative pain in arthroscopic shoulder surgery cannot be easily controlled with analgesics and nerve blocks. This study shows the analgesic effect of interscalene block (ISB) and suprascapular nerve block and axillary nerve block (SSNB + ANB) in patients under patient controlled analgesia (PCA).</P>

도료희석제의 MSDS 신뢰성에 관한 연구

이권섭 ( Kwon Seob Lee ),권현우 ( Hyun Woo Kwon ),한인수 ( In Soo Han ),유일재 ( Il Je Yu ),이용묵 ( Yong Mook Lee ) 한국산업위생학회 2003 한국산업보건학회지 Vol.13 No.3

It has been identified that the content of mixture substances, such as paint thinner, currently marked on the MSDSs is not consistent. There are an increasing number of problems associated with the regular monitoring of workplace environment, based on MSDS; more and more health-related problems are related to the management of harmful chemical substances. Under these circumstances, regarding these harmful substances, there is a compelling need for systematically examining the accuracy of MSDSs, and deeply investigating and evaluating toxic chemicals. From paint thinner Type 2 or higher, 36 types in total of chemical substances were detected. Chemical substances that were commonly found were xylene and trimethylbenzene. Accounting for 81.4%, 57 out of 70 paint thinners out on the market, contained over 1.0% of harmful chemical substances. Out of the seven samples, a small amount of benzene of less than 0.1% was detected. The range of content concentration was from 1.8~74.7 ppm. The results of the analysis show that MSDSs had a 46.5% agreement rate between the detected substances and their content labels, while the warming labels on the products had a agreement rate of 28.3%. In other words, the warning labels had a lower agreement rate than MSDSs. Among the examined paint thinners, 39 products had more than a 50% change in their content of components, accounting for 55.7%, and 55 paint thinners had over a 10% change rate, representing 71.4%.

Distribution of Landslide Deposits and its Implications for the Formation and Evolution of the Dok Island, East Sea

Sang-Mook Lee(이상묵),Yong-Kuk Lee(이용국),Byong-Kwon Park(박병권),Sang-Joon Han(한상준),Bong-Chool Suk(석봉출),Seong-Ryul Kim(김성렬) 대한지질학회 1998 대한지질학회 학술대회 Vol.1998 No.