우리나라의 서양식 치과의료 도입에 관한 연구 3 : 부츠(J. L. Boots)와 맥안리스(J. A. McAnlis)시기의 치과학교실

이주연 서울대학교 의과대학 정신과학교실 1980 精神醫學報 Vol.4 No.3

우리나라의 서양식 치과의료 도입에 관한 연구 1 : 초기 선교치과의사 한 대위(D. E. Hahn)를 중심으로

이주연 서울대학교 의과대학 정신과학교실 1979 精神醫學報 Vol.3 No.2

Enhanced bone formation by controlled growth factor delivery from chitosan-based biomaterials

Lee, Jue-Yeon,Nam, Sung-Heon,Im, Su-Yeon,Park, Yoon-Jeong,Lee, Yong-Moo,Seol, Yang-Jo,Chung, Chong-Pyoung,Lee, Seung-Jin 梨花女子大學校 藥學硏究所 2002 藥學硏究論文集 Vol.- No.11

For the pulpose of obtaining high bone forming efficacy. developlment of chitosan was attempted as a tool useful as ascaffolding device. Porous chitosan matrices, chitosan-poiy(L-lactide) (PLLA) composite matrtices and chitosan coated onPLLA matrices were dealt with in this research. Porous chitosan matrix was fabricated by freeze-drying and cross-linkingaqueous chitosan solution. Porous chitosan matrix combined with ceramics and constituents of extracellular matrices wereprepared and examined for their bone regenerative potential. Composite porous matrix of chitosall-PLLA was prepared bymixing polyiactide with chitosan and freeze-drying. Al1 chitosan based devices demonstrated improved bone formingcapacity by increasing mechanical stability and biocompatibility. Release of platelet-derived growth factor-BB (PDGf-BH )from these matrices exerted significant osteoinductive effect in addition to the higf esteocdElducting capacity of the porouschitosan matrices. The hydrophobic surface of PLLA matrices was modified by chitosan to enhance cell affinity andwettability, The chitosan coafed PLLA matrix induced increased osteoblast attachment as compared with intact PLLAsurface. Overall results in this study demonstrated the usefulness of chitosan as drug releasing scaffolds and as modificationtools for currently used biomaterials to enhance tissue regeneration efficacy. These results may expand the feasibilitr ofcombinatfve strategy of controlled locai drug delivery concept and tissue engineered bone formation in reconstructivetherapy in the field of periodontics, orthopedics and plastic surgery.

Characterization of the surface immobilized synthetic heparin binding domain derived from human fibroblast growth factor-2 and its effect on osteoblast differentiation

Lee, Jue-Yeon,Choo, Jung-Eun,Choi, Young-Sook,Lee, Kuen-Yong,Min, Do-Sik,Pi, Sung-Hee,Seol, Yang-Jo,Lee, Seung-Jin,Jo, In-Ho,Chung, Chong-Pyoung,Park, Yoon-Jeong Wiley Publishers 2007 JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A Vol. No.

<P>Fibroblast growth factor (FGF)-2 regulates a variety of cellular functions, such as proliferation and differentiation, by binding to cell surface FGF receptors (FGFRs) in the presence of heparin proteoglycans. FGF-2 is known as a heparin-binding growth factor, but the localization of the heparin binding site has not been fully investigated until now. We used two potential heparin binding domains of FGF-2, the residues 105–111 (F105, YKRSRYT) and 119–135 (F119, KRTGQYKLGSKTGPGQK). Peptides could be stably immobilized onto the surface of tissue culture plates. Using solid phase binding assays, we demonstrated that both peptides had higher binding affinity toward heparin compared with nonbinding control sequence. The biological significance of these sites was tested by cell attachment and osteoblast differentiation studies. Cell attachment to the peptides F105 and F119 increased in a dose-dependent manner. Heparin and heparinase treatments decreased cell adhesion to both F105 and F119. This demonstrates that both F105 and F119 interact with cell-surface heparan sulfate proteoglycans, suggesting that FGF-2 has two heparin binding sites. In addition, osteoblast differentiation, confirmed by ALPase activity and mineralization, was increased by surface immobilized peptide F105 and F119. Taken together, these heparin binding peptides could be applied as biological agents enhancing osteoblast differentiation as well as surface modification tools in the tissue regeneration area, especially for bone regeneration. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007</P>

Bioactivation of scaffold surface using peptide for enhanced tissue regeneration

Jue Yeon Lee,Young Suk Choi,Jin Sook Seo,Yoon Jung Choi,Jun Beom Park,Sang Chul Lee,Seung Jin Lee,Chong Pyoung Chung,Yoon Jeong Park 한국고분자학회 2008 한국고분자학회 학술대회 연구논문 초록집 Vol.33 No.2

Identification of a cell-penetrating peptide domain from human beta-defensin 3 and characterization of its anti-inflammatory activity

Lee, Jue Yeon,Suh, Jin Sook,Kim, Jung Min,Kim, Jeong Hwa,Park, Hyun Jung,Park, Yoon Jeong,Chung, Chong Pyoung Dove Medical Press 2015 INTERNATIONAL JOURNAL OF NANOMEDICINE Vol.10 No.-

<P>Human beta-defensins (hBDs) are crucial factors of intrinsic immunity that function in the immunologic response to a variety of invading enveloped viruses, bacteria, and fungi. hBDs can cause membrane depolarization and cell lysis due to their highly cationic nature. These molecules participate in antimicrobial defenses and the control of adaptive and innate immunity in every mammalian species and are produced by various cell types. The C-terminal 15-mer peptide within hBD3, designated as hBD3-3, was selected for study due to its cell- and skin-penetrating activity, which can induce anti-inflammatory activity in lipopolysaccharide-treated RAW 264.7 macrophages. hBD3-3 penetrated both the outer membrane of the cells and mouse skin within a short treatment period. Two other peptide fragments showed poorer penetration activity compared to hBD3-3. hBD3-3 inhibited the lipopolysaccharide-induced production of inducible nitric oxide synthase, nitric oxide, and secretory cytokines, such as interleukin-6 and tumor necrosis factor in a concentration-dependent manner. Moreover, hBD3-3 reduced the interstitial infiltration of polymorphonuclear leukocytes in a lung inflammation model. Further investigation also revealed that hBD3-3 downregulated nuclear factor kappa B-dependent inflammation by directly suppressing the degradation of phosphorylated-IκBα and by downregulating active nuclear factor kappa B p65. Our findings indicate that hBD3-3 may be conjugated with drugs of interest to ensure their proper translocation to sites, such as the cytoplasm or nucleus, as hBD3-3 has the ability to be used as a carrier, and suggest a potential approach to effectively treat inflammatory diseases.</P>

Effect of immobilized cell-binding peptides on chitosan membranes for osteoblastic differentiation of mesenchymal stem cells

Lee, Jue-Yeon,Choo, Jung-Eun,Choi, Young-Sook,Shim, In-Kyong,Lee, Seung-Jin,Seol, Yang-Jo,Chung, Chong-Pyoung,Park, Yoon-Jeong Wiley (John WileySons) 2009 Biotechnology and Applied Biochemistry Vol.52 No.1

<P>Two cell-binding domains from FGF-2 (fibroblast growth factor-2) were shown to increase cell attachment and osteoblastic differentiation. Two synthetic peptides derived from FGF-2, namely residues 36-41 (F36; PDGRVD) and 77-83 (F77; KEDGRLL), were prepared and their N-termini further modified for ease of surface immobilization. Chitosan membranes were used in the present study as mechanical supportive biomaterials for peptide immobilization. Peptides could be stably immobilized on to the surface of chitosan membranes. The adhesion of mesenchymal stem cells to the peptide (F36 and F77)-immobilized chitosan membrane was increased in a dose-dependent manner and completely inhibited by soluble RGD (Arg-Gly-Asp) and anti-integrin antibody, indicating the existence of an interaction between F36/F77 and integrin. Peptide-immobilized chitosan supported human bone-marrow-derived mesenchymal-stem-cell differentiation into osteoblastic cells, as demonstrated by alkaline phosphate expression and mineralization. Taken together, the identified peptide-immobilized chitosan membranes were able to support cell adhesion and osteoblastic differentiation; thus these peptides might be useful as bioactive agents for osteoblastic differentiation and surface-modification tools in bone regenerative therapy.</P>

Enhanced bone formation by transforming growth factor-β1-releasing collagen/chitosan microgranules

Lee, Jue-Yeon,Kim, Kyoung-Hwa,Shin, Seung-Yoon,Rhyu, In-Chul,Lee, Yong-Moo,Park, Yoon-Jeong,Chung, Chong-Pyoung,Lee, Seung-Jin Wiley Subscription Services, Inc., A Wiley Company 2006 Journal of biomedical materials research. Part A Vol.a76 No.3

<P>Collagen/chitosan composite microgranules were fabricated as bone substitutes for the purpose of obtaining high bone-forming efficacy. The microgranules have the flexibility to fill various types of defect sites with closer packing. The interconnected pores formed spaces between the microgranules, which allowed new bone ingrowth and vascularization. In addition, the transforming growth factor-beta 1 (TGF-β1) was incorporated into the microgranules in order to improve bone-healing efficacy. The collagen/chitosan microgranules were fabricated by dropping a mixed solution into a NaOH/ethanol solution. TGF-β1 was loaded into the collagen/chitosan microgranules by soaking the microgranules in a TGF-β1 solution. Scanning electron microscopy (SEM) observations and experiments examining the release of TGF-β1 from chitosan and the collagen/chitosan microgranules were performed. SEM was used to examine the cell morphologies on the microgranules and cell proliferation was evaluated using a dimethylthiazole tetrazolium bromide assay. The differentiated cell function was assessed by measuring the alkaline phosphatase (ALPase) activity as well as detecting an osteocalcin assay. The in vivo bone-regeneration experiments were performed using a rabbit calvarial defect model. TGF-β1 was released from the collagen/chitosan microgranules at a therapeutic concentration for 4 weeks. SEM indicated that the seeded osteoblastic cells were firmly attached to the microgranules and proliferated in a multilayer manner. The proliferation of the osteoblasts on the TGF-β1-loaded microgranules was the highest among the different types of microgranules tested. The ALPase activity and osteocalcin level of all the samples increased during the culture period, and the TGF-β1-loaded microgranules had a significantly higher ALPase activity and osteocalcin content than the other microgranules. The TGF-β1-loaded microgranules demonstrated a higher bone-regenerative capacity in the rabbit calvarial defects after 4 weeks than the TGF-β1-unloaded microgranules. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006</P>

Biological effects of a porcine-derived collagen membrane on intrabony defects

Lee, Chang-Kyun,Koo, Ki-Tae,Kim, Tae-Il,Seol, Yang-Jo,Lee, Yong-Moo,Rhyu, In-Chul,Ku, Young,Chung, Chong-Pyoung,Park, Yoon-Jeong,Lee, Jue-Yeon Korean Academy of Periodontology 2010 Journal of Periodontal & Implant Science Vol.40 No.5

Purpose: To prolong the degradation time of collagen membranes, various cross-linking techniques have been developed. For cross-linking, chemicals such as formaldehyde and glutaraldehyde are added to collagen membranes, but these chemicals could adversely affect surrounding tissues. The aim of this study is to evaluate the ability of porous non-chemical cross-linking porcine-derived collagen nanofibrous membrane to enhance bone and associated tissue regeneration in one-wall intrabony defects in beagle dogs. Methods: The second and third mandibular premolars and the first molars of 2 adult beagles were extracted bilaterally and the extraction sites were allowed to heal for 10 weeks. One-wall intrabony defects were prepared bilaterally on the mesial and distal side of the fourth mandibular premolars. Among eight defects, four defects were not covered with membrane as controls and the other four defects were covered with membrane as the experimental group. The animals were sacrificed 10 weeks after surgery. Results: Wound healing was generally uneventful. For all parameters evaluating bone regeneration, the experimental group showed significantly superior results compared to the control. In new bone height (NBh), the experimental group exhibited a greater mean value than the control ($3.04{\pm}0.23\;mm/1.57{\pm}0.59$, P=0.003). Also, in new bone area (NBa) and new bone volume (NBv), the experimental group showed superior results compared to the control (NBa, $34.48{\pm}10.21%$ vs. $5.09{\pm}5.76%$, P=0.014; and NBv, $28.04{\pm}12.96$ vs. $1.55{\pm}0.57$, P=0.041). On the other hand, for parameters evaluating periodontal tissue regeneration, including junctional epithelium migration and new cementum height, there were no statistically significant differences between two groups. Conclusions: Within the limitations of this study, this collagen membrane enhanced bone regeneration at one-wall intrabony defects. On the other hand, no influence of this membrane on periodontal tissue regeneration could be ascertained in this study.

Enhanced bone formation by transforming growth factor-β1-releasing collagen/chitosan microgranules

Lee, Jue-Yeon,Kim, Kyoung-Hwa,Shin, Seung-Yoon,Rhyu, In-Chul,Lee, Yong-Moo,Park, Yoon-Jeong,Chung, Chong-Pyoung,Lee, Seung-Jin 이화여자대학교 약학연구소 2005 藥學硏究論文集 Vol.- No.16

Collagen/chitosan composite microgranules were fabricated as bone substitutes for the purpose of obtaining high bone-forming efficacy. The microgranules have the flexibility to fill various types of defect sites with closer packing. The interconnected pores formed spaces between the microgranules, which allowed new bone ingrowth and vascularization. In addition, the transforming growth factor-beta 1 (TCF-βl) was incorporated into the microsranules in order to improve bone-healing efficacy. The collagen/chitosan microgranules were fabricated by dropping a mixedsolution into a NaOH/ethanol solucon. TCF-β1 was loaded into the collagen/chitosan microgranules by soaking the microgranules in a TCF-βl solution. Scanning electron microscopy (SEM) observations and experiments examining the release of TCF-β1 from chitosan and the collagen/chitosan microgranules were performed. SEM was used to examine the cell morphologies on the microgranules and cell proliferation was evaluated using a dimethylthiazole tetrazolium bromide assay. The differentiated cell function was assessed by measuring the alkaline phosphatase (ALPase)activity as well as detecting an osteocalcin assay. The in vivo bone-regeneration experiments were performed using a rabbit calvarial defect model. TCF-β1 was released from the collagen/chitosan microgranules at a therapeutir concentration for 4 weeks. SEM indicated that the seeded osteoblastic cells were firmly attached to the microgranules and proliferated in a multilayer manner. The proliferation of the osteoblasts on the TCF-β1-loaded microgranules was the high-est among the different types of microgranules tested. The ALPase activity and osteocalcin level of ail the samples increased during the culture period, and the TCF-β1-loaded microgranules had a significantly higher ALPase activity and osteocalcin content than the other microgranules. The TCF-β1-loaded microgranules demonstrated a higher bone-regenerative capacity in the rabbit calvarial defects after 4weeks than the TCF-01-unloaded microgranules.