Selenium 공급방법이 수경재배 토마토의 생장과 Se 흡수에 미치는 영향

이철규(Cheol-Kyu Lee),조경철(Kyung-Cheol Cho),이정현(Jeong-Hyun Lee),조자용(Ja-Yong Cho),서범석(Beom-Seok Seo),양원모(Won-Mo Yang) (사)한국생물환경조절학회 2005 생물환경조절학회지 Vol.14 No.4

토마토 ‘모모타로’를 공시하여 셀레늄 10㎎ㆍL?¹의 관주 및 엽면시비 등의 단용 및 혼용처리가 수경재배 토마토의 생육 및 과실 내 셀레늄 축적 함량에 미치는 영향을 구명하였다. 토마토 종자를 50공 트레이에 파종하여 70일 동안 육묘한 후 유묘를 코코피트 슬라이브를 이용한 수경재배 시스템에 정식하였다. 양액은 일본원예시험장 배양액 표준처방으로 조성하였으며, pH 5.8~6.2와 EC 2.3 ㎎ㆍL?¹ 등으로 조절하여 공급하였다. 셀레늄은 무기태 SeO₂와 sugar fatty acid ester에 킬레이트화 한 유기태 셀레늄을 10 ㎎ㆍL?¹으로 조성하여 관주, 엽면시비, 관주와 엽면시비를 병행하여 처리하였다. 초장, 엽수, 엽면적 및 엽록소 등의 토마토 생장반응은 셀레늄의 엽면시비, 그리고 엽면시비와 관주를 병행한 처리구에서 현저히 증가하였다. 과실 내 셀레늄 축적 함량은 킬레이트화 한 유기태 셀레늄을 엽면시비와 관주를 병행하여 처리한 경우에 0.302 ㎎ㆍL?¹으로 가장 높았다. 무기태와 유기태 셀레늄의 엽면시비와 관주 등의 단용 처리 보다는 혼용 처리가 전반적인 과실 생장과 체내 셀레늄 축적에 효과적이었다. 무기태 설레늄(SeO₂) 보다는 sugar fatty acid ester에 킬레이트화한 유기태 셀레늄 처리가 셀레늄을 함유한 기능성 토마토의 수경재배에 더 효과적이었다. This study was conducted to clarify the effects of supplying methods of selenium on the growth and Se uptake of hydroponically grown tomato plants. Tomato seeds (Lycopersicum esculentum Mill. cv. Momotaro T-93, Daki Seed Co.) were sown in plug tray with fifty holes, and raised for sixty days. Tomato seedlings transplanted to coco fiber slabs were supplied with the nutrient solutions adjusted to EC 2.3 dSㆍm?¹ and pH 5.8~6.2 recommended by the Japanese Horticultural Experiment Station. Selenium forms used were inorganic SeO₂ (here in after referred to Se) and organic selenium chelated with sugar fatty acid ester (here in after referred to chelated-Se). 10 ppm selenium solutions were treated to tomato plants with foliar applications, drenching, and foliar application plus drenching. Growth characteristics in terms of plant height, number of leaves, leaf area and chlorophyll content were significantly increased in the plot of foliar application of Se, and in the plot of foliar application plus drenching of chelated-Se than other plots, respectively. Transported contents of selenium into the tomato fruits were highest as 0.302 ppm in the plot of foliar application plus drenching of chelated-Se. Also, it had tended to be higher in the plot of foliar application plus drenching than in the plots of foliar application or drenching in both of Se and chelated-Se. Foliar application and drenching of organic chelated-Se were effective to produce the functional tomato fruits.

어린이급식관리지원센터의 사업규모에 따른 직원의 직무중요도, 직무수행도, 직무만족도 및 이직의도 분석

서원경(Won Kyung Seo),노정옥(Jeong Ok Rho) 한국식품영양과학회 2020 한국식품영양과학회지 Vol.49 No.9

본 연구는 전국의 어린이급식관리지원센터 중 총 30개 센터, 237명의 직원을 대상으로 2018년 11월부터 12월까지 센터 사업규모를 4억 이하와 5억 이상으로 구분하여 직무중요도, 직무수행도, 직무만족도 및 이직의도를 조사하였다. 직무중요도는 사업규모 5억 이상(4.39)이 4억 이하(4.31) 센터보다 높으며, 직무수행도는 4억 이하(4.23)가 5억 이상(4.19) 센터보다 높은 경향이었다. 중요도와 수행도가 가장 높은 직무는 순회방문 업무이며, 특화사업의 중요도와 수행도가 가장 낮았다. 순회방문 업무는 센터의 설립근거가 되는 업무로 IPA 분석의 4분면 중 A 영역에 해당하였다. 사업규모의 차이 없이 ‘예산관리’의 중요도는 높았으며 식품영양전공자로서 수행의 어려움이 있는 직무임에도 불구하고 높은 수행도를 보였다. 직무만족도는 5억 이상(3.73)이 4억 이하 (3.49) 센터보다 유의적으로 높았으며, 사업규모의 구분 없이 ‘동료와의 관계’의 만족도가 가장 높으며, ‘급여 및 복리후생’이 가장 낮은 수준이었다. 직원들은 본인들이 수행하는 노력에 비해 받는 급여가 공정하지 않다고 생각하며 복리후생제도에 대한 만족도가 매우 낮았는데, 이는 직원들의 이직의도를 높이는 주요 요인으로 판단된다. 특히, 지금보다 나은 직장으로의 이직의도는 4억 이하(3.41)가 5억 이상(3.20) 센터보다 유의적으로 높았다. 직무중요도와 직무수행도 간의 상관관계는 모든 사업규모에서 높은 양의 상관관계를 보였다. 그러나 직무중요도, 직무수행도와 직무만족도 간의 상관관계는 5억 이상 센터에서만 양의 상관관계를 보였다. 이는 5억 이상 센터의 직무만족도가 4억 이하 센터보다 높기 때문이라 생각한다. 직무만족도는 이직의도와 음의 상관관계를 보였는데, 특히 4억 이하 센터 직원의 이직의도는 사업규모가 큰 센터의 직원보다 높았다. 그러나 센터 직원의 잦은 이직은 원장과 교육대상자들의 불만족을 초래할 수 있으며, 교육의 연속성과 질을 낮게 하는 원인이 되므로 이에 대한 대책이 필요하겠다. 이상의 결과, 급식센터 직원의 직무만족도와 이에 따른 이직의도는 사업규모에 따른 편차를 보였다. 따라서 직원의 근로계약 방법 개선을 통해 안정감이 필요하며, 승진시스템 도입을 통해 직무에 대한 책임감을 높일 필요가 있겠다. 특히, 소규모 센터의 경우 직원교육의 업무별 전문화가 필요하며, 규모가 큰 센터와의 협업을 통한 업무 효율화가 필요하겠다. 직원의 높은 직무만족도는 수행도를 높임으로써 센터의 고객인 어린이, 원장, 조리 종사자 및 학부모 등의 만족도에 긍정적으로 영향을 미치므로 식품의약품안전처와 중앙어린이급식관리지원센터는 지역센터 직원의 근무환경에 대한 보다 세심한 관심과 지원이 요구되겠다. This study examined the status of job importance, job performance, job satisfaction, and turnover intention of employees at the Center for Children’s Foodservice Management (CCFSM) according to budget size as well as investigated correlations among these factors. The participants were employees of CCFSM with budgets under 400 million won (n=80) and CCFSM over 500 million won (n=157). The demographic characteristics and status of job importance, job performance, job satisfaction, and turnover intention were assessed using a self-administered questionnaire. The average scores of job importance and performance in CCFSM with budgets under 400 million won were 4.31 and 4.23 points, whereas scores for CCFSM with budgets over 500 million won were 4.39 points and 4.19 points, respectively. Average scores of job satisfaction at CCFSM with budgets over 500 million won were significantly higher than scores at CCFSM with budgets under 400 million won (P<0.01). Average scores of turnover intention at CCFSM with budgets under 400 million won were significantly higher compared to CCFSM with budgets over 500 million won (P<0.05). For CCFSM with budgets under 400 million won, a positive correlation between job importance and job performance was observed (P<0.001). For CCFSM with budgets over 500 million won, positive correlations were observed between job importance and job performance (P<0.001) and job satisfaction (P<0.01) as well as a positive correlation between job performance and job satisfaction (P<0.01). Regardless of budget size, a negative correlation between job satisfaction and turnover intention was detected (P<0.01, P<0.001). Therefore, in order to reduce turnover intention, satisfaction factors with low scores and the working environment should be improved.

Eating habits, physical activity, nutrition knowledge, and self-efficacy by obesity status in upper-grade elementary school students

Kyung Won Kim,Seong Ah Ha,Seo Yeon Lee,Kyung A Kim,Jung Sook Seo,Cheongmin Sohn,HaeryunPark 한국영양학회 2016 Nutrition Research and Practice Vol.10 No.6

BACKGROUND/OBJECTIVES: Childhood obesity has increased in recent decades in Korea. This study was designed to examine differences in the eating habits, physical activity (PA), nutrition knowledge, and self-efficacy of children by obesity status. SUBJECTS/METHODS: Subjects were 5th-grade children from 70 elementary schools in 17 cities nationwide. Two-stage stratified cluster sampling was employed. Survey questionnaire included items related to general characteristics, eating habits, PA, nutrition knowledge and self-efficacy. Excluding incomplete responses, 3,531 data were analyzed using SPSS. Subjects were categorized into overweight∙obesity (OW) and normal weight (NW) groups based on body mass index percentiles for age by sex. RESULTS: A total of 21.5% of subjects was overweight or obese. There were significant differences in gender, perceived stress, perception of body shape, body satisfaction, and interest in weight control between the OW and NW groups (P < 0.001). With respect to eating habits, the OW group ate breakfast (P < 0.05) and snacks (P < 0.01) less frequently, ate bigger meals (P < 0.001), and demonstrated less desirable behaviors during meals (P < 0.05 in boys) compared to the NW group. The OW group participated in less PA than the NW group, especially boys. OW boys spent less time walking during weekdays (P < 0.05) or the weekend (P < 0.001), spent more time being sedentary during weekdays or the weekend (P < 0.001), and exercised a fewer number of days (P < 0.01). For girls, the OW group spent more time being sedentary during the weekend (P < 0.01) and exercised a fewer number of days by walking or bicycle riding (P < 0.05) than the NW group. Nutrition knowledge was not significantly different between the OW and NW groups. Self-efficacy (P < 0.01 in boys), especially PA self-efficacy (P < 0.01), was significantly lower in the OW than NW group. CONCLUSIONS: This study revealed differences in eating habits, PA, and self-efficacy between OW and NW children. Obesity management programs for children need to focus on increasing self-efficacy, modifying eating habits, and increasing PA.

Laparoscopy-assisted distal gastrectomy for early gastric cancer and laparoscopic cholecystectomy for gallstone with situs inversus totalis: a case report

Kyung Won Seo,Ki Young Yoon 대한외과학회 2011 Annals of Surgical Treatment and Research(ASRT) Vol.81 No.6

We report our case of laparoscopy-assisted distal gastrectomy with D1 + β lymph node dissection for a patient with early gastric cancer and laparoscopic cholecystectomy for gallstone with situs inversus totalis. A superficial elevated lesion was found on the lesser curvature of the antrum. The preoperative diagnosis was cStage IA (cT1, cN0, cH0, cP0, cM0). A 1 cm-sized gallstone was found in the fundus through upper abdominal ultrasound. A laparoscopy-assisted distal gastrectomy with standard D2 lymph node dissection for early gastric cancer and laparoscopic cholecystectomy was successfully performed by not shifting the monitor to the left and right and not changing operator’s position without additional blood loss and time. The number of retrieved lymph nodes was 36. We have not found any abnormal course of blood vessels except for the right/left inversion. Billroth I reconstruction was performed through end-to-side anastomosis. Based on a histopathological examination, a 1.5 × 1.5 cm, submucosal (sm3), moderately differentiated adenocarcinoma (pT1, pN0, sH0, sP0, sM0, stage IA) was diagnosed. The postoperative course was favorable and the patient was discharged on postoperative day 7.

Amylase, lipase, and volume of drainage fluid in gastrectomy for the early detection of complications caused by pancreatic leakage

Kyung Won Seo,Ki Young Yoon,Sang Ho Lee,Yeon Myung Shin,Kyung Hyun Choi,Hyun Yong Hwang 대한외과학회 2011 Annals of Surgical Treatment and Research(ASRT) Vol.81 No.6

Purpose: Pancreatic leakage is a serious complication of gastrectomy due to stomach cancer. Therefore, we analyzed amylase and lipase concentrations in blood and drainage fluid, and evaluated the volume of drainage fluid to discern their usefulness as markers for the early detection of serious pancreatic leakage requiring reoperation after gastrectomy. Methods: From January 2001 to December 2007, we retrospectively analyzed data from 24,072 patient samples. We divided patients into two groups; 1) complications with pancreatic leakage (CG), and 2) no complications associated with pancreatic leakage (NCG). Values of amylase and lipase in the blood and drainage fluid, volume of the drainage fluid, and relationships among the volumes, amylase values, and lipase values in the drainage fluid were evaluated, respectively in the two groups. Results: The mean amylase values of CG were significantly higher than those of NCG in blood and drainage fluid (P < 0.05). For lipase, statistically significant differences were observed in drainage fluid (P < 0.05). The mean volume (standard deviation) of the drained fluid through the tube between CG (n = 22) and NCG (n = 236) on postoperative day 1 were 368.41 (266.25) and 299.26 (300.28), respectively. There were no statistically significant differences between the groups (P = 0.298). There was a correlation between the amylase and lipase values in the drainage fluid (r = 0.812, P = 0.000). Conclusion: Among postoperative amylase and lipase values in blood and drainage fluid, and the volume of drainage fluid, the amylase in drainage fluid was better differentiated between CG and NCG than other markers. The volume of the drainage fluid did not differ significantly between groups.

Enhanced electrical properties of PEDOT:PSS films using solvent treatment and its application to ITO-free organic light-emitting diodes

Seo, Yoon Kyung,Joo, Chul Woong,Lee, Jonghee,Han, Joo Won,Lee, Dong Jin,Entifar, Siti Aisyah Nurmaulia,Kim, Soukyoon,Cho, Nam Sung,Kim, Yong Hyun Elsevier 2017 Journal of luminescence Vol.187 No.-

<P><B>Abstract</B></P> <P>Highly conductive poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) films are prepared by introducing a new solvent 2-ethoxyethanol and are optimized by acid-free solvent post treatment. The behavior of samples are investigated with various coating conditions. The change of electrical performance for 2-ethoxyethanol added PEDOT:PSS films with various post treatment methods is studied. Upon post treatment, the sheet resistance greatly decreases attributed to a structural change with removal of insulating PSS in the film. Based on these conductive films, we demonstrate efficient ITO-free green phosphorescent organic light-emitting diodes (OLEDs). The efficiency of OLEDs with post-treated PEDOT:PSS electrodes is greater than that of OLEDs with untreated PEDOT:PSS electrodes. The results illustrate a promising future for flexible, low-cost, ITO-free OLEDs employing PEDOT:PSS electrodes optimized by 2-ethoxyethaol with acid-free solvent post treatment.</P>

Future changes due to model biases in probabilities of extreme temperatures over East Asia using CMIP5 data

Seo, Ye‐,Won,Yun, Kyung‐,Sook,Lee, June‐,Yi,Lee, Yang‐,Won,Ha, Kyung‐,Ja,Jhun, Jong‐,Ghap John Wiley Sons, Ltd 2018 International journal of climatology Vol.38 No.3

<P><B>ABSTRACT</B></P><P>This study examines the performances of 31 global climate models in the Coupled Model Inter‐comparison Project 5 (CMIP5) in terms of probability density functions (PDFs) for maximum (<I>T</I>max) and minimum (<I>T</I>min) air temperatures over East Asia in the present and CMIP5‐model projected future changes. In general, most of models well reproduce warm‐season peak for both <I>T</I>max and <I>T</I>min but exhibit large inter‐model spread for simulating cold‐season peak, especially for <I>T</I>min. Minimum values of <I>T</I>min and <I>T</I>max are more strongly dependent upon model selection than maximum values of them. For the last 25 years of the 21st century, under the Representative Concentration Pathways 4.5 scenario, models project shifts toward warmer values in the PDFs of <I>T</I>max and <I>T</I>min and broadening in the shape of PDFs. Models with warm biases in PDFs tend to show larger shifts in temperature changes, but seasonal mean temperature biases do not affect to future changes. It is notable that the broadening of PDFs in the future influences temperature extreme events. Using the changes in probabilities of heat waves as one of extreme temperature events by comparing multi‐model ensemble (MME) and models with good performance of PDFs, this study shows that MME tends to overestimate its duration. Our findings suggest that future changes in temperature extremes projected by models are strongly come from the biases detected in those models when simulating present extreme temperature PDFs. Therefore, correcting the intrinsic biases of models rather than seasonal mean correction is necessary to reduce the uncertainties in predicting future changes in temperature extremes.</P>

Anti-colitis effect of <i>Lactobacillus sakei</i> K040706 via suppression of inflammatory responses in the dextran sulfate sodium-induced colitis mice model

Seo, Seunghwan,Shin, Ji-Sun,Lee, Woo-Seok,Rhee, Young Kyoung,Cho, Chang-Won,Hong, Hee-Do,Lee, Kyung-Tae Elsevier 2017 Journal of Functional Foods Vol.29 No.-

<P><B>Abstract</B></P> <P>Lactobacillus strains are used in the functional food industry as promising probiotics with purported beneficial effects. We recently reported on the immunostimulatory effects of <I>Lactobacillus sakei</I> K040706 (K040706) in macrophages and in a cyclophosphamide-induced immunosuppression model. And this study assessed the anti-inflammatory effects and molecular mechanisms involving K040706 in a mouse model of colitis with dextran sodium sulfate (DSS). K040706 significantly attenuated the clinical signs and histological characteristics in DSS-induced colitic mice, which are well-correlated with the restoration of tight junction protein expression and the significant reduction of immune cell infiltration and expression of ICAM-1 and VCAM-1. Moreover, K040706 reduced the abnormal expression of pro-inflammatory mediators, such as iNOS, TNF-α, IL-1β, and IL-6, and the activation of NF-κB, STAT3 and TLR4. K040706 also improved the composition of intestinal microbiota. These results suggest K040706 has shown significant anti-inflammatory effects in a DSS-induced colitis model and has the potential to treat IBD.</P> <P><B>Highlights</B></P> <P> <UL> <LI> K040706 attenuated DSS-induced colitis severity and epithelial injury. </LI> <LI> K040706 suppressed the DSS-induced expression of pro-inflammatory mediators. </LI> <LI> K040706 downregulated the DSS-induced activation of TLR4/NF-κB/STAT3 signaling. </LI> <LI> K040706 improved the intestinal microbiota composition. </LI> <LI> K040706 has potential as a treatment option for inflammatory bowel disease. </LI> </UL> </P>

Low-frequency pulsed electromagnetic field pretreated bone marrow-derived mesenchymal stem cells promote the regeneration of crush-injured rat mental nerve

Seo, NaRi,Lee, Sung-Ho,Ju, Kyung Won,Woo, JaeMan,Kim, BongJu,Kim, SoungMin,Jahng, Jeong Won,Lee, Jong-Ho Medknow PublicationsMedia Pvt Ltd 2018 Neural regeneration research Vol.13 No.1

<P>Bone marrow-derived mesenchymal stem cells (BMSCs) have been shown to promote the regeneration of injured peripheral nerves. Pulsed electromagnetic field (PEMF) reportedly promotes the proliferation and neuronal differentiation of BMSCs. Low-frequency PEMF can induce the neuronal differentiation of BMSCs in the absence of nerve growth factors. This study was designed to investigate the effects of low-frequency PEMF pretreatment on the proliferation and function of BMSCs and the effects of low-frequency PEMF pre-treated BMSCs on the regeneration of injured peripheral nerve using <I>in vitro</I> and <I>in vivo</I> experiments. In <I>in vitro</I> experiments, quantitative DNA analysis was performed to determine the proliferation of BMSCs, and reverse transcription-polymerase chain reaction was performed to detect S100 (Schwann cell marker), glial fibrillary acidic protein (astrocyte marker), and brain-derived neurotrophic factor and nerve growth factor (neurotrophic factors) mRNA expression. In the <I>in vivo</I> experiments, rat models of crush-injured mental nerve established using clamp method were randomly injected with low-frequency PEMF pretreated BMSCs, unpretreated BMSCs or PBS at the injury site (1 × 10<SUP>6</SUP> cells). DiI-labeled BMSCs injected at the injury site were counted under the fluorescence microscope to determine cell survival. One or two weeks after cell injection, functional recovery of the injured nerve was assessed using the sensory test with von Frey filaments. Two weeks after cell injection, axonal regeneration was evaluated using histomorphometric analysis and retrograde labeling of trigeminal ganglion neurons. <I>In vitro</I> experiment results revealed that low-frequency PEMF pretreated BMSCs proliferated faster and had greater mRNA expression of growth factors than unpretreated BMSCs. <I>In vivo</I> experiment results revealed that compared with injection of unpretreated BMSCs, injection of low-frequency PEMF pretreated BMSCs led to higher myelinated axon count and axon density and more DiI-labeled neurons in the trigeminal ganglia, contributing to rapider functional recovery of injured mental nerve. These findings suggest that low-frequency PEMF pretreatment is a promising approach to enhance the efficacy of cell therapy for peripheral nerve injury repair.</P>

윤상인두근 기능 이상에 의한 중증 연하장애 환자에서 풍선확장치료 및 보툴리눔독소 주사치료의 효과: 증례 보고

Won Kyung Lee,Han Gil Seo,Min Yong Seong,Jiwoon Yeom,Woo Hyung Lee,Tai Ryoon Han,오병모 대한연하장애학회 2017 대한연하장애학회지 Vol.7 No.2

Objective: To investigate changes of swallowing function after ballooning dilatation (BD) and the Botox injection (BI) into the cricopharyngeus muscle in patients with severe dysphagia. Method: Nine severe dysphagic patients with cricopharyngeal dysfunction (CPD) who underwent BD and/or BI into the cricopharyngeal muscle were retrospectively reviewd. Patients who had severe dysphagia (Functional Oral Intake Scale (FOIS)≤2) after at least 3 months of the conventional swallowing therapy were included by a thorough review of medical records with videofluoroscopic swallowing study (VFSS). Before and after several interventions (BD and/or BI), swallowing function was evaluated using VFSS. Result: Among 9 patients, 5 underwent both BD and BI, and the other 4 patients underwent only BD. Four among 9 cases showed that interventions were effective. Of the 5 cases with both BD and BI, 2 cases were effective for treatment of CPD. In all the effective 4 cases, pyriform sinus residue seemed to be related with FOIS. Of those cases, one case had long-term effect (more than 4 months) and the other 3 case had short term effect (less than 4 months). Conclusion: Interventions were effective in 4 among 9 cases with severe CPD and the therapeutic effect was sustained for more than 4 months. The results suggest that in CPD patients, the BD or BI into UES could be considered in selected patients.