렛트 Adenosine Dearninase에 관한 연구

김우현,이정섭,이병국,박진우,김형로 의과학연구소 1989 全北醫大論文集 Vol.13 No.1

냉장 닭고기의 저장 및 시판동안 미생물학적 품질에 관한 연구

김창렬,고대희,강어진 서강전문대학 2000 산업과학연구 Vol.2 No.-

Microbiological evaluations of refrigerated chicken during a commercial chicken processing, storage, and handling at between March and July, 1998 were assessed. Aerobic plate counts (APC) of chicken wings obtained from retail store during storage of 7 days (2±2℃) were 5.80 log unit, which was the highest levels compared to chicken legs, chicken breasts, and whole chicken. APC and gram-negative bacterial counts (GNC) of whole chicken during refrigerated (4℃) storage under temperature abuse for lhr at 35℃ were 6.711og unit and 6.22 log unit, respectively. However, APC and GNC of whole chicken during refrigerated (10℃) storage under temperature abuse for lhr at 35℃ were not microbiological acceptable after storage of 4 days. Whole chicken obtained from a commercial chicken processing after evisceration significantly (P< 0.05) increased APC and GNC compared to those of after defeathering, before air chilling, and after final processing.

대유행 인플루엔자(H1N1 2009)에서 신속항원검사 양성률

김영근,김효열,어영,전진경 대한감염학회 2010 감염과 화학요법 Vol.42 No.2

There are few datas on the diagnostic accuracy of rapid antigen test for pandemic influenza A (H1N1). We evaluated the performance of rapid antigen test for the diagnosis of pandemic influenza A (H1N1). During the period from 21 August 2009 to 20 September 2009, 451 patients with influenza-like symptom underwent the rapid antigen test (SD Influenza Antigen, Standard Diagnostics, Yongin, Korea) and real-time reverse transcriptase-polymerase chain reaction (rRT-PCR) at the same time. Rapid antigen test results were positive for 65 of 90 patients with the positive results for pandemic influenza A (H1N1) on rRT-PCR assay. The sensitivity of the rapid antigen test was 72.2% (95% CI, 61.8-81.1) and the specificity was 100% (95% CI, 99.0-100). The positive predictive value was 100% (95% CI, 94.5-100) and negative predictive value was 93.5% (95% CI, 90.6-95.8).

Incidence of Clostridioides difficile Infections in Republic of Korea: A Prospective Study With Active Surveillance vs. National Data From Health Insurance Review & Assessment Service

Kim Jieun,Myung Rangmi,Kim Bongyoung,Kim Jinyeong,Kim Tark,Lee Mi Suk,Kim Uh Jin,Park Dae Won,Kim Yeon-Sook,Lee Chang-Seop,Kim Eu Suk,Lee Sun Hee,Chang Hyun-Ha,Lee Seung Soon,Park Se Yoon,Choi Hee Jun 대한의학회 2024 Journal of Korean medical science Vol.39 No.12

Background: Since the emergence of hypervirulent strains of Clostridioides difficile, the incidence of C. difficile infections (CDI) has increased significantly. Methods: To assess the incidence of CDI in Korea, we conducted a prospective multicentre observational study from October 2020 to October 2021. Additionally, we calculated the incidence of CDI from mass data obtained from the Health Insurance Review and Assessment Service (HIRA) from 2008 to 2020. Results: In the prospective study with active surveillance, 30,212 patients had diarrhoea and 907 patients were diagnosed with CDI over 1,288,571 patient-days and 193,264 admissions in 18 participating hospitals during 3 months of study period; the CDI per 10,000 patientdays was 7.04 and the CDI per 1,000 admission was 4.69. The incidence of CDI was higher in general hospitals than in tertiary hospitals: 6.38 per 10,000 patient-days (range: 3.25–12.05) and 4.18 per 1,000 admissions (range: 1.92–8.59) in 11 tertiary hospitals, vs. 9.45 per 10,000 patient-days (range: 5.68–13.90) and 6.73 per 1,000 admissions (range: 3.18–15.85) in seven general hospitals. With regard to HIRA data, the incidence of CDI in all hospitals has been increasing over the 13-year-period: from 0.3 to 1.8 per 10,000 patient-days, 0.3 to 1.6 per 1,000 admissions, and 6.9 to 56.9 per 100,000 population, respectively. Conclusion: The incidence of CDI in Korea has been gradually increasing, and its recent value is as high as that in the United State and Europe. CDI is underestimated, particularly in general hospitals in Korea.

Poster Session : PS 0033 ; Cardiology : Impact of Metabolic Syndrome and Obesity on Clinical Outcomes After Primary Percutaneous Coronary Intervention for ST-Segment Elevation Myocardial Infarction

( Uh Jin KIM ),( Seunghun LEE ),( Myung Ho JEONG ),( Ju Han KIM ),( HyukJin PARK ),( Ji Eun KIM ),( Youngkeun AHN ),( Jong Hyun KIM ),( Shung Chull CHAE ),( Young Jo KIM ),( Seung Ho HUR ),( In Whan S 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: The correlation between obesity and metabolic syndrome (MetS) remains unclear. This study aims to investigate impact of obesity phenotype on clinical outcomes with ST-segment elevation myocardial infarction (STEMI). Methods: We analyzed in-hospital mortality and major adverse cardiac events (MACE) of 2,606 obese patients who underwent primary percutaneous coronary intervention (PCI) in the Korea Acute Myocardial Infarction Registry from November 2005 to October 2010. Patients were divided into two groups: unhealthy (any of the Mets components) obesity (BMI =25 kg/m2) and healthy (none of the Mets components) obesity. Results: The unhealthy obesity group was composed of 703 patients (27%) and healthy obesity group 1,903 patients (73%). Unhealthy obesity was more frequent in female (25.9% vs. 10.1%, P<0.001) and multi-vessel disease (53.1% vs. 47.6%, P=0.012). There were no signifi cant differences in other baseline characteristics, angiographic and procedural fi ndings, and prescribed medications between two groups. The overall incidence of complications after PCI (13.1% vs. 10.7%, P=0.097) and in-hospital mortality (1.9% vs. 1.6%, P=0.521) were similar in unhealthy obesity group compared with healthy obesity group. By the multivariate Cox regression analyses, the presence of Mets in obese patients was not an independent factor for predicting the adjusted MACE rate at one-month (HR 1.19, 95% CI 0.56-2.54, P=0.645), six-month (HR 1.48, 95% CI 0.94-2.32, P=0.088), and twelve-month (HR 1.10, 95% CI 0.75-1.61, P=0.622). Conclusions: Obesity phenotype is not an independent prognostic factor for patients with STEMI who undergoing primary PCI. The risk stratifi cation by presence or absence of Mets is not useful in these patients.

Clinical Characteristics and Risk Factors for Mortality in Critical COVID-19 Patients Aged 50 Years or Younger During Omicron Wave in Korea: Comparison With Patients Older Than 50 Years of Age

Shi Hye Jin,Yang Jinyoung,Eom Joong Sik,Ko Jae-Hoon,Peck Kyong Ran,Kim Uh Jin,Jung Sook In,Kim Seulki,Seok Hyeri,Hyun Miri,Kim Hyun Ah,Kim Bomi,Joo Eun-Jeong,Cheong Hae Suk,Jun Cheon Hoo,Wi Yu Mi,Kim 대한의학회 2023 Journal of Korean medical science Vol.38 No.28

Background: The coronavirus disease 2019 (COVID-19) pandemic has caused the death of thousands of patients worldwide. Although age is known to be a risk factor for morbidity and mortality in COVID-19 patients, critical illness or death is occurring even in the younger age group as the epidemic spreads. In early 2022, omicron became the dominant variant of the COVID-19 virus in South Korea, and the epidemic proceeded on a large scale. Accordingly, this study aimed to determine whether young adults (aged ≤ 50 years) with critical COVID-19 infection during the omicron period had different characteristics from older patients and to determine the risk factors for mortality in this specific age group. Methods: We evaluated 213 critical adult patients (high flow nasal cannula or higher respiratory support) hospitalized for polymerase chain reaction-confirmed COVID-19 in nine hospitals in South Korea between February 1, 2022 and April 30, 2022. Demographic characteristics, including body mass index (BMI) and vaccination status; underlying diseases; clinical features and laboratory findings; clinical course; treatment received; and outcomes were collected from electronic medical records (EMRs) and analyzed according to age and mortality. Results: Overall, 71 critically ill patients aged ≤ 50 years were enrolled, and 142 critically ill patients aged over 50 years were selected through 1:2 matching based on the date of diagnosis. The most frequent underlying diseases among those aged ≤ 50 years were diabetes and hypertension, and all 14 patients who died had either a BMI ≥ 25 kg/m2 or an underlying disease. The total case fatality rate among severe patients (S-CFR) was 31.0%, and the S-CFR differed according to age and was higher than that during the delta period. The S-CFR was 19.7% for those aged ≤ 50 years, 36.6% for those aged > 50 years, and 38.1% for those aged ≥ 65 years. In multivariate analysis, age (odds ratio [OR], 1.084; 95% confidence interval [CI], 1.043–1.127), initial low-density lipoprotein > 600 IU/L (OR, 4.782; 95% CI, 1.584–14.434), initial C-reactive protein > 8 mg/dL (OR, 2.940; 95% CI, 1.042–8.293), highest aspartate aminotransferase > 200 IU/L (OR, 12.931; 95% CI, 1.691–98.908), and mechanical ventilation implementation (OR, 3.671; 95% CI, 1.294–10.420) were significant independent predictors of mortality in critical COVID-19 patients during the omicron wave. A similar pattern was shown when analyzing the data by age group, but most had no statistical significance owing to the small number of deaths in the young critical group. Although the vaccination completion rate of all the patients (31.0%) was higher than that in the delta wave period (13.6%), it was still lower than that of the general population. Further, only 15 (21.1%) critically ill patients aged ≤ 50 years were fully vaccinated. Overall, the severity of hospitalized critical patients was significantly higher than that in the delta period, indicating that it was difficult to find common risk factors in the two periods only with a simple comparison. Conclusion: Overall, the S-CFR of critically ill COVID-19 patients in the omicron period was higher than that in the delta period, especially in those aged ≤ 50 years. All of the patients who died had an underlying disease or obesity. In the same population, the vaccination rate was very low compared to that in the delta wave, indicating that non-vaccination significantly affected the progression to critical illness. Notably, there was a lack of prescription for Paxlovid for these patients although they satisfied the prescription criteria. Early diagnosis and active initial treatment was necessary, along with the proven methods of vaccination and personal hygiene. Further studies are needed to determine how each variant affects critically ill patients.

A New Assay Method for Ornithine Aminotransferase

Kim, Uh Hyun,Kim, Hyung Rho,Lee, Gye Jeong,Park, Jin Woo 생화학분자생물학회 1986 BMB Reports Vol.13 No.4

An attempt was made to develop a new assay method for ornithine aminotransferase which catalyze the transfer of δ-amino group of ornithine to α-ketoglutamate-γ-semialdehyde and glutamate. Glutamate-γ-semialdehyde(or pyrroline-5'-carboxylic acid), one of the reaction products was found to produce a colored compound showing a peak absorbance at 510 ㎚ with ninhydrin in hot acidic solution, whereas ornithine and glutamate production and color intensity indicated that the enzyme activity could be properly assayed by reading the change of absorbance at 510 ㎚. The enzyme activity measured by new assay method was widely distributed in mouse tissues, especially high in small intestine, kidney and liver, and was mainly present in cytoplasmic fraction of small intestine and in mitochondrial fraction of liver. The enzyme activity showed increasing tendency in liver and kidney with age, in contrast to decreasing tendency in small intestine. The enzyme from hepatic mitochondrial fraction had nearly identical properties to that from intestinal cytoplasmic fraction, such as heat stability in the presence of pyridoxal phosphate, pH activity curves, Km values, for ornithine and α-ketoglutarate, suggesting that they are rather an identical-enzyme than isoenzymes.

Properties of Poly ( ADP - ribose ) Sythetase from the Sprout of Glycin Max Linne ( Soybean )

Kim, Uh Hyun,Won, Jong Jin,Kim, Hyung Rho 생화학분자생물학회 1986 BMB Reports Vol.12 No.4

The effect of pH, mono-and divalent canons, sodium dodecyl sulfate, N-ethylmaleimide, ouabain, sodium azide and several steroid hormones on Ca^(2+)-ATPase activity of sarcoplasmic reticulum isolated from conger eel (Astroconger myriaster) muscle were examined. The results obtained were as follows: 1. Ca^(2+)-ATPase of conger eel muscle sarcoplasmic reticulum showed its maximum activity at pH 7.4. 2. Monovalent cations used in this study increased Ca^(2+)-ATPase activity. The order of effectiveness of monovalent canons tested at 100mM concentration in increasing the activity of ATPase is K^+ $gt; Na^+ $gt; Li^+ $gt; NH₄^+, Ca^(2+)-ATPase activity was inhibited by divalent cations with the following order of effectiveness at 0.5 mM concentration: Cd^(2+) $gt; Co^(2+) $gt; Sr^(2+) $gt; Mn^(2+) $gt; Zn^(2+) 3. Ca^(2+)-ATPase activity was inhibited significantly by sodium dodecyl sulfate and N-ethylmaleimide but not affected by ouabain and sodium azide. 4. Ca^(2+)-ATPase activity was slightly inhibited by 17α-hydroxypregnenolone and β-estradiol, and severely inhibited by hydrocortisone at all concentrations used in this study. 5. β-pregnan-3β-ol-20-one, dehydroisoandrosterone and corticosterone activated Cat+-ATPase activity slightly at low concentrations and at high concentrations, 5α-pregnan-3β-ol-20-one did not exert any influence on ATPase activity but dehydroisoandrosterone and corticosterone inhibited enzyme activity severely.

Hyperferritinemia as a Diagnostic Marker for Severe Fever with Thrombocytopenia Syndrome

Kim, Uh Jin,Oh, Tae hoon,Kim, Bansuk,Kim, Seong Eun,Kang, Seung-Ji,Park, Kyung-Hwa,Jung, Sook-In,Jang, Hee-Chang IOS PRESS 2017 Disease markers Vol.2017 No.1

The Survival Rate of Korean Patients with COPD with or without Acute Exacerbations

Uh, Soo-Taek,Lee, Ji-Yeon,Koo, So-Mi,Kim, Yang-Ki,Kim, Ki-Up,Park, Jong-Sook,Park, Sung-Woo,Jang, An-Soo,Kim, Do-Jin,Choi, Jae-Sung,Na, Joo-Ok,Suh, Ki-Hyun,Kim, Yong-Hoon,Park, Choon-Sik The Korean Academy of Tuberculosis and Respiratory 2011 Tuberculosis and Respiratory Diseases Vol.70 No.6

Background: Chronic obstructive pulmonary disease (COPD) is characterized by air low limitation, which is one of the leading causes of mortality worldwide. There have been many studies on survival rates in the world literature, but there have been few reports regarding the survival rate in Korean patients with COPD. Acute exacerbation is regarded as a risk factor for mortality in patients with COPD. The purpose of this study was to investigate the survival rate and the effect of acute exacerbations on the survival rate of Korean patients with COPD. Methods: A total of 502 COPD patients who were diagnosed on the basis of history and lung function tests were enrolled in this study. The frequency of acute exacerbations, body mass index (BMI), C-reactive protein (CRP) and pulmonary hypertension were analyzed. Results: The 3- and 5-year survival rates were 98% and 83%, respectively. The median survival time was 78 months. The median survival time was 55 months in 322 patients with one or more acute exacerbations. The 3- and 5-year survival rates were significantly lower in the 322 patients with one or more acute exacerbations than in those without any. The mortality rate was significantly higher in patients with CRP>3 mg/L than in those with CRP ${\leq}3$ mg/L (p<0.005); it was significantly higher in patients with pulmonary hypertension than in those without it (p<0.01). Conclusion: Because the 5-year survival rate is 83% in Korean patients with COPD, the management of stable patients with COPD should focus on the prevention of acute exacerbations.