In vitro anti-bacterial and anti-oxidative activities of lactic acid bacteria-bioconversioned almond extract

Kiha Lee,Hyunwoo Ahn,Jaehee Choi,Kwang-geun Lee 한국식품영양과학회 2020 한국식품영양과학회 학술대회발표집 Vol.2020 No.10

Quantitative Comparison of Activity Calculation Methods for the Selection of Most Reliable Radionuclide Inventory Estimation

Kiha Hwang,Sangchul Lee,Kun Jai Lee,Chanwoo Jeong,Sangmyeon Ahn,Taewook Kim,Kyoungdoek Kim,Y.H.HERR 한국방사성폐기물학회 2003 한국방사성폐기물학회 학술논문요약집 Vol.2003 No.11

다구찌방법을 이용한 다축 동시 PID 제어시스템의 제어이득 조정

이기하(Kiha Lee),김종원(Jongwon Kim) Korean Society for Precision Engineering 1999 한국정밀공학회지 Vol.16 No.6

이중외피의 경계적 표현특성

이기하(Lee, Kiha),송지현(Song, Jihyun),윤갑근(Yoon, Gabgeun) 한국디자인지식학회 2014 디자인지식저널 Vol.29 No.-

건축은 과거의 단편적이고 일률적인 정형적 형태, 공간구성에서 벗어나 다양하고 역동적이며, 독창적인 형태로 나타나면서 기존에 자리 잡고 있던 건축표현 방법과는 다르게 시도되고 있다. 그리고 외부와 내부가 분리되는 보편적인 경향에서 벗어나 다양한 성격의 경계면과 그 내부에서 일어나는 공간간의 상호작용 등을 통하여 구분이나 경계가 없는 건축적 통합체로서의 새로운 가능성을 만들어낸다. 또한, 이와 더불어 최근 환경적인 요소와 심미적인 요소가 중요시됨에 따라 이중외피 또한 형태생성의 한 부분으로서 대두되고 있다. 본 논문에서는 2000년대 이후의 현대건축의 이중외피가 공간과 어떠한 경계적 상호연관성을 가지고 기능적 , 구조적, 연속적인 방법으로 표현되어 지는지에 집중하여 연구를 진행 하였다. 그 결과, 이중외피의 시, 지각적인 새로운 요소가 건축의 내, 외부와 맞물리면서 기존의 외피로서의 분리적인 개념이 아닌 이질적 요소들 간의 통합과 결합을 통하여 영역이 확장되며 의미가 재해석되고 강조되어 내부와 외부를 소통하는 결과가 도출되었으며 다양한 요소간의 만남으로 이중외피가 새로운 영역을 만들고 있음을 찾아볼 수 있었다. As architecture has appeared in diverse, dynamic, and unique styles out of segmental, uniform, and inflexible spatial composition, we have pursued different architectural expression methods. Then, out of the universal tendency to divide the internal from the external, we aim to create new possibilities for architectural synthesis through spatial interaction occurring at different boundaries and inside. Also, these days, as we have focused on environmental and aesthetic factors, the double skin has appeared as part of a morphological generation. This study looked at boundary correlations between the double skin of contemporary architecture since the 2000s and space, concentrating on its functional, structural, and successive expression. As a result, it was discovered that as the visual and cognitive factors of the double skin connected the inside and outside of buildings, spaces were expanded and their meanings were reinterpreted through integration and combination of heterogeneous factors, which led to communication between the interior and exterior. It was concluded that through a combination of diverse factors, the double skin created new areas for growth.

Investigation of different basal medium used for stem cell conditioned medium regarding the effect on mice parthenotes development

Kihae Ra,Hyun Ju Oh,Min Jung Kim,Geon A Kim,Sung Keun Kang,Jeong Chan Ra,Byeong Chun Lee 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11

Recent research on stem cell conditioned medium (CM) has been revealed that CM could influence on the embryo development when supplemented to in vitro culture medium. However, the optimal basal medium for CM production has not determined although it is the fundamental factor of CM. The purpose of this study is to examine the effect of human derived adipose stem cell CM (hASC-CM) with different basal medium on mice embryo development after parthenogenetic activation (PA). hASC-CM was collected from 2 kinds of serum free basal medium, DMEM and KSFM, respectively on day 5 from the culture of hASC isolated from human fat tissue. Intra-peritoneal injection of PMSG and hCG was conducted into 7-week-old ICR mice for superovulation. The oocytes were recovered from the oviductal ampulla, 18 h after hCG injection, and denuded using 0.1% hyaluronidase. PA of oocytes was conducted with KSOM media including strontium chloride. The parthenotes were in vitro cultured in 3 groups: 100% KSOM (Control), 75% KSOM + 25% DMEM or KSFM without FBS (DMEM or KSFM group) and 75% KSOM + 25% hASC-CM from DMEM or KSFM (DMEM-CM or KSFM-CM group). Cleavage rate was assessed after 2 days post IVC and blastocyst formation rate was evaluated after 6 days post IVC both using stereomicroscope. Total cell number of blastocysts was counted by Hoechst staining. 1way ANOVA from Graphpad prism 5 was used for statistical analysis and the values are presented as means ± standard error of mean. As a result, blastocyst formation rate of DMEM-CM group (16.09±3.32%, P<0.05) was significantly lower than control and DMEM group (34.43±2.89% and 34.49±5.34%, P<0.05) but cleavage rate and total cell number of blastocysts showed no significant difference among groups. In case of KSFM, there was no significant difference in cleavage rate, blastocyst formation rate and total cell number of blastocysts among the control, KSFM group and KSFM-CM group. The sort of basal medium used for the CM collection affected the development of parthenotes during in vitro culture differently. Therefore, further research should be conducted to find out the alternative basal medium of CM able to improve the embryo development. This research was supported by Nature Cell (#550-20170028), Cooperative Research Program of RDA (CCAR, #PJ013954022018), Research Institute for Veterinary Science and the BK21 plus program.

The effect of intravenous injection of human adipose derived stem cell conditioned medium on ovulation and subsequent embryo development in aged mice

Kihae Ra,Geon A Kim,Hyun Ju Oh,Sung Keun Kang,Jeong Chan Ra,Byeong Chun Lee 대한수의학회 2017 대한수의학회 학술대회발표집 Vol.2017 No.-

한국형 2.75 인치 로켓 추진기관 개발

강기하(Kiha Kang),이용범(Yongbum Lee),염용일(Yongyeol Yeom),방기복(Gibok Bang),양영준(Youngjun Yang) 한국추진공학회 2014 한국추진공학회지 Vol.18 No.3

In this paper, the development of unique model of the 2.75 inch rocket propulsion system is described. Recently developed korean 2.75 inch rocket propulsion system shows the improvement of a flame stability resulted from a change in the configuration of propellant grain, and of an incidental ignition protection function using the EMI(electromagnetic interference) filter on ignition system. Moreover it is shown that a directional flight stability is improved by increasing the number of fins and changing the nozzle configuration. Static firing test and thermal shock test were conducted for the validation before flight, and flight test of 210 rounds of rockets was conducted to verify the trajectory uniformity. In addition, intellectual property issues can be overcome with the unique korean 2.75 inch rocket motor as well as the performance improvement.

북한이탈주민 맞춤형 구강건강증진 프로그램의 효과

전기하 ( Kiha Jeon ),이수영 ( Suyoung Lee ) 대한예방치과·구강보건학회 2018 大韓口腔保健學會誌 Vol.42 No.4

Objectives: The purpose of this study was to evaluate the customized oral health promotion program for North Korean defectors (NKDs). Methods: Using a pre-survey comprising phases 1-5 of the PRECEDE-PROCEED model, a customized oral health promotion program was created. The participants, a total of 129 NKDs, were allocated to the experimental group (n=64; 43 females and 21 males) or the control group (n=65; 46 females and 19 males). After the interventions, phases 7 to 9 of the PRECEDE-PROCEED model were executed to determine the effect of the program. Results: The results for phase 7 revealed high satisfaction with the program, with an average of 2.89 out of 3 points. In phase 8, concerning the impact evaluation, it was found that oral health beliefs and oral health knowledge were improved, with statistically significance differences before and after the experiment. Phase 9, concerning the outcome evaluation, showed that there were statistically significant differences in the average within groups before and after education in oral health beliefs (OHB), O’Leary index scores, and CPI. In terms of the difference between the groups, there were statistically significant improvement in OHB, O’Leary index scores, and CPI after the education program. Conclusions: The oral health promotion program developed in this study has made a positive contribution to improve the oral health status of NKDs.

Expression, reconstruction and characterization of codon-optimized carbonic anhydrase from Hahella chejuensis for CO2 sequestration application.

Ki, Mi-Ran,Min, Kiha,Kanth, Bashistha Kumar,Lee, Jinwon,Pack, Seung Pil Springer-Verlag 2013 Bioprocess and biosystems engineering Vol.36 No.3

<P>The high production of functional carbonic anhydrase (CA) is required for practical CO2 sequestration application mediated by CA. Here, the synthetic gene based on Escherichia coli codon usage of new alpha-type CA (HC-aCA) of Hahella chejuensis, a Korea marine microorganism, was highly expressed in E. coli. We obtained a high yield of functional HC-aCA by denaturing/refolding process and incorporating zinc ion into its active site. The refolded HC-aCA displayed a half-deactivation temperature of 60 A degrees C with maximal activity at 50 A degrees C, and had high pH stability in alkali condition with maximal activity at pH 10.0. The esterase activity of HC-aCA almost doubled at high salt concentration ranging from 0.67 to 2.0 M NaCl. HC-aCA catalyzed the conversion of CO2 to CaCO3 as calcites form in the presence of Ca2+. The refolded HC-aCA could be a promising candidate for the development of efficient CA-based CO2 sequestration processes.</P>

우리나라의 중점녹색기술수준 조사・분석 및 시사점

홍미영(Mi-Young Hong),황기하(KiHa Hwang),홍정석(Jung Suk Hong),이경재(Kyong-Jae Lee) 한국기술혁신학회 2013 기술혁신학회지 Vol.16 No.2

우리나라 정부는 ‘저탄소 녹색성장’을 새로운 국가발전 패러다임으로 제시한 이래로 ‘녹색기술 연구개발 종합대책(’09.1)’을 통해 27대 중점 녹색기술을 도출하는 등 녹색성장의 중심으로 녹색기술 개발전략을 수립?추진해 왔다. 본 연구에서는 대규모 녹색기술 전문가 집단이 참여하여 델파이 조사 기법을 활용한 27대 중점 녹색기술 내 총 131개의 전략제품?서비스 기술을 대상으로 기술수준 조사를 실시하였다. 2011년 기준으로 중점 녹색기술 전체에 대한 주요 5개국의 기술수준은 세계최고기술보유국(미국) 대비 EU(99.4%), 일본(95.3%), 한국(77.7%), 중국(67.1%) 순이며, 한국은 5개국 중 4위를 차지하였다. 세계최고기술보유국(미국)과 한국과의 기술격차년수는 4.1년으로 EU(3.9년), 일본(3.1년)에는 뒤져 있는 반면, 중국에는 2.1년 앞선 것으로 조사되었다. 우리나라의 기술수준이 가장 높은 중점 녹색기술은 ‘개량형 경수로 설계 및 건설기술(90.1%)’이며, 이어서 ‘실리콘계 태양전지의 고효율 저가화 기술 (85.0%)’, ‘고효율 저공해 차량기술(84.5%)’ 순으로 나타났다. 중점 녹색기술의 투자유형에 따른 기술수준은 단기(85.0%), 중기(77.3%), 장기(71.1%) 집중형 순이며, 기술수준이 낮을수록 중장기적인 투자를 요하는 것으로 나타나 전반적인 투자유형 설정은 적절한 것으로 조사되었다. Korea government has established and pursued green technology development strategy as the core of green growth, for example, withdrawal of 27 key green technologies through ‘green technology research and development comprehensive plan (’09.1)’ since ‘low carbon green growth’ was proposed as a new national development paradigm. In this study, we performed the Delphi survey of technology levels of 131 strategic product and service technologies derived from 27 key green technologies, utilizing large-scale group of green technology experts. The survey of technology level among main five nations resulted in the world’s leading nation (US) versus EU (99.4%), Japan (95.3%), Korea (77.7%), China (67.1%) and Korea was ranked fourth. The technology gap between the world’s leading nation (US) and Korea is 4.1 years behind EU (3.9 years) and Japan(3.1 years), but 2.1 years earlier than China. For our nation, key green technologies with high technology level are ‘improved light water reactor (90.1%)’, ‘silicon-based solar cell (85.0%)’, ‘high-efficiency low-emission car (84.5%)’ in order. Depending on the investment type of key green technologies, technology level is represented as short-term (85.0%), mid-term (77.3%) and long-term (71.1%) in order, indicating that lower technology level requires midto long-term investment and that the investment type is set appropriate.