전립선암에서 TGFβl, TGFβ2 발현과 Ki-67 표지지수의 예후적 가치

장혁준,김기권,이경섭 大韓泌尿器科學會 1999 Korean Journal of Urology Vol.40 No.5

직장 카르시노이드 종양에서 Ki-67 발현의 예후적 중요성

홍수민 ( Su Min Hong ),김유선 ( You Sun Kim ),문정섭 ( Jung Seop Moon ),김진남 ( Jin Nam Kim ),오명기 ( Myoung Ki Oh ),권선옥 ( Sun Ok Kwon ),정성연 ( Seong Yeon Jeong ),홍성우 ( Seong Woo Hong ),강윤경 ( Yun Kyung Kang ) 대한소화기학회 2013 대한소화기학회지 Vol.61 No.2

Background/Aims: Rectal carcinoid tumors can be resected with endoscopy, and it is important to assess their prognostic factors. We evaluated the potential of Ki-67 expression as a prognostic factor in rectal carcinoid tumors. Methods: We retrospectively reviewed the medical records of 37 patients with rectal carcinoid tumors who got endoscopic resection from January 2001 to January 2011 at Inje University Seoul Paik Hospital. We analyzed their endoscopic and histologic findings, Ki-67 expression, clinical outcome, and prognosis. Results: The mean age (±SD) of the patients was 56.3±10.7 years, and the male : female ratio was 3.6:1. The mean tumor size was 0.5±0.4 cm, 33 patients showed grade 1 tumors (89.2%) and the average Ki-67 expression was 0.7±1.2%. Thirty five patients underwent endoscopic mucosal resection, and two required endoscopic submucosal dissection. Eight patients had positive margins after resection, but no cases of lymphovascular invasion were identified. The median follow-up duration was 21.4±25.4 months, and no recurrences were observed. Conclusions: In low grade rectal carcinoid tumors which are lack of central depression on colonoscopy, the expression of a molecular marker of malignant potential, Ki-67, was low. Therefore, endoscopic resection seemed to be a safe and effective treatment for these tumors.

전립선암에서 p53, bcl-2 단백의 발현 및 Ki-67 표지지수와 예후인자와의 상관 관계

김대곤,이경섭,김기권 大韓泌尿器科學會 2001 Korean Journal of Urology Vol.42 No.8

中風病辨證診斷標準에 의한 臨床的 硏究

任長信,文祥官,高昌男,權正男,曺基湖,金永錫,裵亨燮,李京燮 경희대학교 1997 慶熙醫學 Vol.13 No.3

대량재해시 방사선학적 검사에 의한 개인식별예

권귀향,최득린,강신몽,이혜경,김기정 大韓法醫學會 1992 대한법의학회지 Vol.16 No.1

There cases of mass disaster, KAL accident in Tripoli, express bus accident and park cemetry accident in Korea, were referred to the National Institute of Scientific Investigation, Seoul, Korea for individual identification (ID). All the cases were thoroughly investigated to establish the most scientifically sound ID possible. In addition to evidence from fingerprint, dental, anthropological, visual, pathological, blood typing and superimpose according to the case, X-ray studies were attempted. In KAL case, 52 of 59 were identified by comparison radiography. Differential diagnosis was accomplished in 2 misidentified bodies in express bus case by radiography. Radiologic examination also revealed good characteristics in park cemetry case, however, did not satisfactorily contribute to ID because of restricted information.

흰쥐에서 탄수화물 종류가 운동시 당 및 지질대사에 미치는 영향

권태동,전중기,민경선,김형렬,장응찬 대구대학교 기초과학연구소 1998 基礎科學硏究 Vol.15 No.2

The purpose of this study was to investigate the effects of glucose, sucrose, and glucose polymer(GP) ingestion on the carbohydrate and lipid metabolism during treadmill running in rats. Rats were randomly assigned to normal, control and carbohydrate groups. Carbohydrate group was divided into glucose, sucrose and GP ingested rats. The experiment was carried out after overnight fasting. Rats were administrated either normal saline(control) or carbohydrates solutions of formulated glucose(6%), sucrose(6%) and GP(6%) via polyethlene tube to stomach before treadmill run. Exercise was loaded by a treadmill run at the speed of 1 km/hour for 30, 60 and 120 minutes. The results of experiment were as follows: Plasma glucose levels were decreased in proportion to the exercise time in control, but increased at treadmill run 30 minutes and then gradually decreased with the lapse of the exercise time in carbohydrates compared with normal rats. It decreased in control, glucose, sucrose and GP compared with normal rats in decreasing order at treadmill run 120 minutes(% change of plasma glucose level of control, glucose, sucrose and GP compared with normal rats were 67, 74, 87 and 91 in plasma glucose level at treadmill run 120 min., respectively). Plasma lactate levels were increased by the exercise loading compared with normal in control and carbohydrates ingested rats. It increased in control, sucrose and GP in increasing order compared with normal rats, at treadmill run 120 minutes(% change of plasma lactate level of control, glucose, sucrose and GP compared with normal rats were 167, 185, 149 and 114 in plasma lactate level at treadmill run 120 min., respectively). Plasma FFA levels had tendency to increase by the exercise loading compared with normal in control and carbohydrates ingested rats. It showed increase in the sucrose, glucose, GP and control rats in Increasing order compared with normal rats at treadmill run 120 minutes(% change of plasma FFA level of control, glucose, sucrose and GP compared with normal rats were 151, 153, 166 and 152 in plasma FFA level at treadmill run 120 min., respectively). Plasma acetoacetate levels were higher in control compared with carbohydrates ingested rats at treadmill run 30, 60 and 120 minutes. Plasma beta-hydroxybutyrate levels increased by exercise compared with normal rats in control and carbohydrates ingested rats at treadmill run 30, 60, 120 minutes. Plasma insulin levels were decreased in proportion to the exercise time in control compared with normal rats, but in carbohydrates ingested rats increased at treadmill run 30 minutes and then gradually deceased with the lapse of the exercise time compared with normal. In carbohydrates ingested rats, plasma insulin levies were higher than control. Hematocrit did not show any difference between control and carbohydrates rats at treadmill run 30, 60 and 120 minutes. From these experimental results, it is suggested that the exhaustion of skeletal muscle glycogen, the hypoglycemia, the overproduction of lactate and dehydration is delayed in carbohydrates ingested rats. GP of the carbohydrates is considered as the most effective for these effects. Therefore, intake of carbohydrates before exercise, especially GP seems to increase performance in the prolonged moderate exercise.

옥정호수계에 있어서 화학적 및 생물학적 오염도 지표조사

권태영,강경홍,박기남 전주대학교 자연과학종합연구소 1997 전주대학교 자연과학연구소 학술논문집 Vol.10 No.3

A inveatigation in water system in Okjung lake for the pollution loading and self purification capacity, the nitrate concentration, as a pollution loading index, was 0.2-0.3mM in Sumjin river and Sangchi stream. Imsil stream was mainly pollution source in water system of Okjung lake. Nitrate concentration of Okjung lake was 0.06-0.1mM. This results was mean that the capacity of self-purification of Okjung lake have in reserve.

말초혈액에서 Tg mRNA에 대한 역전사 중합효소 연쇄 반응법의 갑상선 재발암의 분자생물학적 진단

권성일,박기룡,김현영,신채희,임영찬,최영식,박요한,이강대,장희경,이재화,염하용 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.4

연구배경: 갑상선암은 다른 조직에 발생한 암에 비해 비교적 천천히 자라므로 대부분 예후가 양호하지만, 일부에서는 주위 조직으로 침윤하거나 혹은 원격 전이로 인하여 치명적인 결과를 초래할 수 있다. 갑상선전절제술 및 131^I 제거술 후 경과 관찰시 갑상선암의 재발과 전이의 진단에 있어 131^I 스캔과 혈청 Tg의 측정이 현재 임상에서 가장 많이 이용되고 있으나 이 방법에는 여러 가지의 결점이 있다. 최근 Tg mRNA에 대한 RT-PCR법을 이용한 여러 연구결과는 131^I 스캔과 혈청 Tg 측정의 결점을 보완할 수 있는 좋은 보조적인 진단법으로 이용할 수 있을 가능성을 제시하였다. 이에 말초혈액에서 측정한 Tg mRNA에 대한 RT-PCR법이 갑상선 절제술 및 방사성요드 치료 후 갑상선암의 재발 및 전이 유무의 진단에 유용한가를 알아보고자 이 연구 시행하였다. 방법: 분화된 갑상선암으로 진단된 후 갑상선전절제술을 시행받고 방사성요드 치료를 받은 환자 중 현재까지 한차례에 이상 추적 방사성요드 전신 스캔을 시행하고 추적관찰이 가능했던 유두선암 35예, 여포선암 5예를 대상으로 연구를 시행하였다. 대상군은 131^I 스캔 소견상 음성인 군(Group Ⅰ), 잔여조직이 있는 군(Group Ⅱ), 국소전이가 있는 군(Group Ⅲ), 및 원격전이 군(Group Ⅳ)으로 구분하였다. 정상 대조군은 갑상선질환이 없는 10예의 건강인으로 하였다. 대상환자의 말초혈액을 이용한 Tg mRNA에 대해 특이적인 primer를 이용하여 RT-PCR 및 nested RT-PCR을 시행하였다. 결과: 본 연구 결과는 다음과 같다. 1) 131^I 스캔 소견상 음성인 군 21예 중 1예에서 Tg가 양성소견을 보였다. Anti Tg Ab가 양성인 4예 모두 Tg가 음성을 보였다. 잔여조직이 있거나 국소전이 및 원격전이를 보인 군 19예 중 Tg가 양성인 경우는 4예였으나, Tg mRNA는 전예에서 양성이었다. 2) 131^I 스캔에서 국소 및 원격전이 소견을 보인 8예 중 4예에서 Tg가 음성으로 131^I 스캔과 혈청 Tg 사이에 불일치 소견을 보였다. 3) 말초혈액에서 특이적인 primer를 이용하여 RT-PCR 및 nested RT-PCR을 시행한 결과 대상군 40예 및 정상 대조군 10예 모두에서 Tg mRNA가 양성을 보였다. 결론: 본 연구에서 갑상선 절제술 및 방사성요드 치료 후 갑상선암의 재발 및 전이 유무를 평가함에 있어 역전사 중합효소 연쇄 반응법을 이용한 Tg mRNA 측정의 의의는 재평가되어야 한다고 생각된다. Background: Differentiated thyroid cancer is the most common endocrine malignancy. Despite advances in the treatment of thyroid cancer, disease recurrence and metastasis may occur in as many as 20% of patients, and so continues to pose major problems in its clinical management. Serum thyroglobulin (Tg) measurements, by immunoassay, are used to detect residual or recurrent thyroid cancer following thyriod ablation. However, the usefulness of immunoassay is limited by both the requirement for thyroid hormone withdrawal, to attain optimal test sensitivity, and interference by the antithyroglobulin antibody (Anti-Tg Ab). Recent studies have reported the clinical usefulness of reverse transcription-polymerase chain reaction (RT-PCR) detection of Tg mRNA in the peripheral blood of patients with differentiated thyroid carcinomas. We performed this study to evaluated the usefulness RT-PCR of Tg mRNA in peripheral blood of patients with thyroid carcinoma following a total thyroidectomy and radioiodine ablation therapy. Methods: Forty cases that underwent a total thyroidectomy and radioiodine ablation therapy were included in this study. Of the 40 patients, 35 were papillary carcinomas and 5 were follicular carcinomas. Ten normal control subjects were also studied. Tg mRNA was extracted. Then RT-PCR and nested RT-PCR, were run with specific Tg primers. Concurrently, DNA sequencing of the isolates was carried out to prove the isolates were identical to the nucleotide sequence of the Tg. Results: The Tg was detected in 4 of 19 patients, with either a residual thyroid bed, or metastasis, on a 131^I whole body scan and in 1 of 21 patients with a negative radioiodine scan. Surprisingly, the Tg mRNA was detected in all the patients and normal controls. Conclusion: From our results we can not recommend Tg mRNA, detected by RT-PCR in peripheral blood, as a tumor marker superior to that of the Tg serum level. We consider an intensive re-evaluation of the method is required before considering its clinical applications (J Kor Soc Endocrinol 17:501∼513, 2002).

重金屬Ion과 Chelate를 形成하는 Aminocarboxylic Acid 樹脂의 合成

李學沂,金祐埴,민경은,權五亮 慶北大學校 1980 論文集 Vol.29 No.-

Crosslinked polyacrylamide was prepared from acrylamide and divinylbenzene by the radical polymerization in methanol to prepare crosslinked polyvinylamine by the Hofmann reaction. Crosslinked aminocarboxylic acid resin was finally prepared by the reaction of crosslinked polyvinylamine and chloroacetic acid. The adsorption of crosslinked aminocarboxylic resin for Cu^+2, Zn^+2, Ni^+2, and Co^+2 varies with pH and the resin shows a high adsorption in the range of pH 6-7. The order of adsorption for heavy metal ions was Cu^+2>Zn^+2>Ni^+2>Co^+2 at the above pH region.

양로원과 요양원 간호사의 업무내용 비교 : 전국의 무료 양로 · 요양시설 중심으로

성기월,신임희,이경희,권경희 노인간호학회 2005 노인간호학회지 Vol.7 No.1

Purpose: The study was performed to compare the content of their jobs of the nurses working in the free elderly home with free nursing home in the whole country. Method: The subjects consisted of 138 nurses working in elderly home and nursing home. The data was collected from Jan. 1st to Feb. 22th 2005 using a structured questionnaire that included general characteristics, content of tasks which the nurse dose every day, health program, perceived job satisfaction and pay satisfaction scale. The collected data was analyzed by the SPSS(version 11.5) program including descriptive statistics, x^(2)-test. Result: On the nurses in the free elderly homes and nursing homes, there were many patient diagnosing hypertension, arthritis and dementia. Recently the death rate in elderly home and nursing home was highly increased. The mean period of nurses working in elderly home and nursing home were 3.75 years and 2.76 years. Experience of staying with the elderly was 70.9% on nurses working in elderly home. and 73.2% on nurses working in nursing home. Daily allotment of the nurses working in elderly home and nursing home were health assessment, medication care and eating care. The highest priority rank of nursing care of the nurse working in elderly home and nursing home was health assessment. Mean number of the patient in daily nursing care of the nurse residing in elderly home and nursing home was referral to hospital on 1-5persons/day and then transfer care on 1-6persons/day. The highest health care program was an exercise program. Conclusion: The resident nurses in the free elderly homes and nursing homes have had various types of tasks, and for their security a system for job satisfaction was needed.