Focal adhesion kinase and src expression in premalignant and malignant skin lesions

( Ju Yeon Choi ),( Jae Yun Lim ),( Han Saem Kim ),( Jung Min ),( Jung In Kim ),( Hyun Min Seo ),( Sang Hyeon Hwang ),( Chong Won Choi ),( Yoon Hwan Kim ),( Jin Hee Sohn ),( Hyunjoo Lee ),( Won Serk Ki 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: Focal adhesion kinase (FAK) and Src are non-receptor tyrosine kinases. FAK and Src play a critical role in inducing malignant transformation in tumor cells. Objectives: We performed immunohistochemical staining for total and phosphorylated forms of FAK and Src, to evaluate the role of FAK and Src in the development of premalignant and malignant skin lesions. Methods: A total of 59 facial skin samples (30 actinic keratoses, 10 Bowen``s diseases, 13 squamous cell carcinomas and six perilesional skins) were immunohistochemically stained for Ki-67, total (t) and phosphorylated (p) form of FAK and Src. Methods: A total of 59 facial skin samples (30 actinic keratoses, 10 Bowen``s diseases, 13 squamous cell carcinomas and six perilesional skins) were immunohistochemically stained for Ki-67, total (t) and phosphorylated (p) form of FAK and Src. Results: Cells positive for t-Src, p-Src-y530, t-FAK and pFAK-s722 were detected in premalignant intra-epithelial lesions (PELs) and squamous cell carcinomas (SCCs), but not in the perilesional skin. There was a tendency towards high correlation between Ki-67 and t-FAK or pFAK-s722, suggestive of the active role of FAK in cell proliferation. Conclusion: Our findings of higher t-Src and p-Src-y530 positive cells in PELs, as compared to SCCs (with higher Ki-67 level), are suggestive of the other role of Src in tumor formation and progression, which requires further investigation.

방울토마토중 Dichlofluanid 및 Iprodione의 생산단계별 잔류농약 경시변화

최규일,성기용,정태균,이주환,허장현,고광용,이규승 한국환경농학회 2002 한국환경농학회지 Vol.21 No.4

방울토마토의 생산단계에서의 잔류허용기준 설정과 수확후 저장기간중 경시변화 및 세척에 의한 잔류농약의 감소양상을 파악하였다. 해당약제를 안전사용기준의 기준량 및 배량을 각각 시설재배 포장에서 살포후 10일간 잔류양상을 조사하였고, 실온 및 냉장조건 하에서의 저장실험 및 세척에 따른 전류농약의 잔소량을 파악하여 생산단계부터, 출하, 저장, 소비단계까지의 잔류량을 예측할 수 있는 모델개발을 위한 자료로 이용하고자 연구를 수행하였다. Dichlofluanid와 iprodione은 포장조건에서 반감일수가 기준량 2.2, 3.3일 이었으며, 배량은 3.5, 5.4일로 나타났다. 저장조건하에서의 약제별 반감기는 포장조건보다 잔류량의 감소속도가 느린 것으로 나타나 반감일수가 증가하였고, 세척방법에 의한 잔류농약의 감소량을 조사한 결과 세제 사용시의 평균제거율은 dichlofluanid는 73.7%, iprodione은 64.3%로 나타났고, tap-water 사용시에는 dichlofluanid 73.5%, iprodione 63.5%로 나타났다. 이러한 결과는 작물의 생산단계에서 구한 합리적인 희귀식을 이용하여 잔류농약의 경시변화를 파악하고, 저장 및 세척과정을 거쳐 최종 소비단계에서의 잔류농약의 수준을 평가할 수 있었다. We studied the residual patterns of two fungicides, dichlofluanid and iprodione, in cherry tomato greenhouse after applying with the recommended and double dose. Also, the degradation patterns during storage periods of up to ten days were compared between at room temperature (20℃) and at cold temperature (4℃). Removal rates of fungicides by washing with tap-water and detergent solution (0.1%, 0.2%) were measured. Half-lives of dichlofluanid and iprodione in greenhouse cherrytomato were 2.2~3.5 and 3.3~5.4 days, respectively. During the storage period, the residues were dissipated more slow. Removal rates were 62.8~80.3% by tap-water, 60.4~83.1% by 0.1% detergent solution, and 65.3~77.6% by 0.2% detergent solution. So, we can predict of terminal residues from cultivation period to marketing, storage and consuming.

간흡충 감염증의 진단에서 피내반응 검사의 유용성 검토

최재철,오승환,정해진,신정환,박기형,이은엽,장철훈 대한임상미샐물학회 2003 Annals of clinical microbiology Vol.6 No.2

한국인 임신부에 있어서 영양소의 역가측정

최기영,김웅철,박기현,배도환 중앙대학교 의과대학 의과학연구소 1982 中央醫大誌 Vol.7 No.2

Animal and human studies indicated that inadequate diet for a prolonged period during pregnancy could injure mother, child, or both. In order to prevent this problem, maternal nutyitional status during pregnancy is very important and the evaluation of nutrition during pregnancy have been preceded by many authors in another countries. In Korea, nutrient intake studies of the pregnant women and nutrition surveys in adult by biochemical method have been performed, but there are no data nutrition surveys of Korean pregnant women have been made by biochemical method. In this regard, an exact biochemical titer of the nutrients and the degree of nutrient deficits in Korean pregnant women are needed. In this study, the mean titer of some nutrients and the degree of nutrient deficits by biochemical method in Korean pregnant women were assessed. The objected materials were consisted of 30 cases which had visited the departmant of Obstetrics & Gynecology, Chung Ang University Hospital for antenatal care(32wk-40wk) from March, 1981 to June, 1981. The cases with twin pregnancy, and the cases complicated with materal chronic wasting disease, diabetes, hydroamniosis, hypertensive disorder in pregnancy were excluded in this study. The results were as follows: 1. Mean titer of 6 hours urine execretion of thiamine, riboflavin and N'-methylniacinamide in Korean pregnant women was 44㎍, 466㎍, and 2.33㎎. 2. Mean titer of serum vitamin A, carotene, vitamin C, total protein, calcium, phosphorus, iron and total lipid was 48.1㎍, 154.7㎍, 0.8㎎, 7.0g, 9.8㎎ 3.5㎎, 93㎍, and 578㎎. 3. Percentage of nutrient deficits in Korean pregnant women based on the mean titer of Korean adults was as follow; thiamine-20%, N'-methylniacinamide-7%, total protein-5%, calcuim13%, iron-19%. 4. No cases were deficient in riboflavin, carotene, phosphorus, total lipid and the mean titer of riboflavin and carotene in Korean pregnant women were high compared with thw mean titer of Korean adults.

고온·알칼리성 Bacillus sp.의 Catabolite Repression 저항성 변이주로부터 Cellulase 및 Xylanase의 생산

최수철,정영철,강갑석,심기환,성낙계 慶尙大學校 1990 論文集 Vol.29 No.2

고온·알칼리성 cellulase 및 hemicellulase 생성 미생물을 분리할 목적으로 pH 10.3의 PY-CMC배지에서 55℃로 3회 연속 직접배양하여 효소 생성능이 우수한 20여균주를 분리하였고, 그 중에서 2개균주를 최종 선정하여 균학적 성상을 조사한 결과 고온·알칼리성 Bacillus속과 거의 일치하였다. 선정, 동정된 고온·알칼리성 Bacillus sp. F204와 F1020은 α-1, 4-glucose 등에서는 효소 생합성이 왕성하였으나, glucose와 같은 단당류에서는 효소가 전혀 유도되지 않았는데, 이 두 균주는 carbon catabolote repression을 받고 있었다. 2-deoxy glucose는 야생주에서는 강력한 repressor로 작용하였고, 야생주의 catabolite repression을 해제 시키는데 antimetabolite로 작용한다는 것이 밝혀졌다. NTG(500㎍/㎖, 50℃, 20∼60분)와 UV(2분) 처리후 2-DG(20mM)에 직접배양하여 강력한 catabolite repression resistant strain인 M-211과 M-367을 분리하였다. M-211과 M-367은 2-DG를 energy원으로 이용하였으며, glucose에 대하여 CMCase는 1.5%, avicelase 및 xylanase는 약 1.0%까지 저항성을 보였다. 또한 두개의 변이주의 효소 활성은 CMCase 2배, avicelase 1.5배 및 xylanase3배의 증가를 보였으며, 각 효소생합성을 위한 gene expression은 cAMP-independent transcription을 하는 것으로 나타났다. Cellulase and xylanase synthesis of thermophilic alkalophilic Bacillus sp. F204 and F1020 isolated in our lab, were repressed by glucose and induced by compounds structurally related to α-1,4 linkage (avicel cellulose and xylan et. al.). Catabolite repression-resistant mutants were obtained through treatment of NTG(500㎍./㎖), UV(2 min) and enrichment culture in 2-deoxy glucose (20 mM) containing PY-glucose on the wild type strain. T재 catabolite repression resistant mutants were not repressed by glucose and produced cellulase complex and xylansase when in grown in the presence of 1.0% glucose and 30mM 2-DC, which were repressors in wild type. Enzyme production was greater with the mutants (CMCase of 2 times, avicelase of 1.5 times, xylanase of 3 times) than with the wild type strains under various test conditions. T재 mutants M-211 and M-367 were potential catabolite repression resistant mutants and their gene expression was cAMP independent transcription.

SS41 용접재의 피로크랙 진전거동에 관한 연구

최병기,권택용,정환규 朝鮮大學校 機械技術硏究所 2000 機械技術硏究 Vol.3 No.1

SS41 is welded automatically and is investigated some effects of the welding residual stress in the growth and propagation of fatigue crack, so as to study the fatigue behaviour in the welding residual stress field. The summarized results are as follows; 1) In case of the load range is constant, as the stress ratio is changing to 0.1, 0.33 and 0.5 the propagation life is constant but the growth life decreases. And than, when maximum load or minimum load is constant, as the stress ratio increases the growth life and propagation life. 2) It was shown that the fatigue crack propagation ratio da/dN was almost constant regardless of the stress ratio change at constant load amplitude and that the larger stress ratio, the slower was the fatigue crack propagation ratio. 3) The opening ratio U is influenced by Kmax but it isn't only the function of Kmax because data range is very large. 4) The growing and propagation fatigue crack in the welding residual stress field is similar to original specimen after passing the original residual stress field.

지리 정보 시스템을 위한 DXF 화일 변환기의 설계 및 구현

최영환,오병우,한기준 건국대학교 산업기술연구원 1997 건국기술연구논문지 Vol.22 No.-

Recently, a Geographic Information System (GIS), which stores a large volume of graphical data into a computer system and manages them efficiently according to user's needs, is widely used. However, since various graphics data formats are now used in geographical information systems and DXF (Data eXchange Format) is used for national topographical maps in Korea, it is necessary to develop a DXF file converter that converts a DXF file into another data file to increase the usage of DXF files. In this paper, we design and implement a DXF converter which convets a DXF file into a PostScript file since the latter is one of the most powerful graphics formats and can be used for various printers directly. It consists of the file manipulation module, the DXF file manipulation module, and PostScript file generation module. In order to develop the DXF converter, we first analyze each of the file formats (i.e., DXF and PostScript formats) and then implement and test the three modules. Finally, we develop the complete DXF file converter by combining the three modules.

MSE를 이용한 동영상의 장면전환검색에 관한 연구

최우진,김단환,정기봉,오무송 조선대학교 전자정보통신연구소 2002 電子情報通信硏究所論文誌 Vol.5 No.1

Multimedia information includes image information, sound information, character information and various informations. Difficulty follows to look for image which user wants by existent retrieval method. Therefore, introduce MSE(Mean Square Error) by index method for efficient practical use of video information in this research and propose scene change detection retrieval method of video. This image data for diagonal line direction color value of schedule pixel draw, (By j^(*)i matrix to Matrix A, i is frame number, j stored cost that express diagonal line Image height of frame to Matrix A). So that can grasp whole structure of video, do sampling in still image, Made user can grasp whole structure of video, do sampling in still image. Made user grasp scene change point of total video on one eye. If MSE of each frame exceeds critical value, search the frame by scene change.

화학 중합형 콤포짓트 레진의 착색도 측정

최기열,박태환 국립서울정신병원 1987 臨床硏究論文集 Vol.1987 No.-

壓挫시킨 家兎 軟骨의 生存

최동원,송중원,한기환,강진성,정재홍 大韓成形外科學會 1990 Archives of Plastic Surgery Vol.17 No.2

The deviated nose regardless whether it is due to traumatic or congenital origin results in troublesome symptoms coming from airway obstruction. Numerous methods for straightening the severely deviated septum have been described by many authors but none is wholly satisfactory. A new method that the crooked septal cartilage is being flattened by crushing and reimplanting into the septal pocket is introduced. The authors are desirous of knowing about the fate of the crushed cartilage graft. Forty rabbits weighing 2 to 3 kg were divided into 2 groups, septal group and conchal group. The cartilages were obtained from the nasal septum and ear; these cartilages were placed under the paraspinal subcutaneous tissue. Prior to paraspinal iplantation the obtained cartilage was halved; one piece of cartilage being crushed by Cottle's cartilage crusher before its placement into the recipient site and the other was placed without crushing. The cartilage specimens were removed at 6 and 12 weeks postoperatively and these were examined by the naked eyes and light microscope. The results were as follows: 1. The fissures and cleavages of cartilage developed during crushing process are restored to normal by 6 weeks postoperatively. 2. All of the ujncrushed cartilages remained viable in the subcutaneous pockets. The crushed cartilages not only appeared viable but also induced a new cartilage formation beneath the perihondrium at 12 weeks postoperatively and the thickness being increased compaired to the cartilage before crushing. 3. Crushed cartilage remained flat at 12 weeks postoperatively. 4. The degree of strength of the crushed cartilage is increased due to new cartilage formation and fibrosis of perichondrium at 12 weeks postoperatively. These findings support the clinical impression that the crushed autogenous cartilage is viable and can be reshaped according to the contour of the recipient site.