Analysis of Fracture Surface Morphology and Study of Seepage Diffusion Characteristics

Zheng Li,Kun Mao,Jie Liu,Kexin Zhang 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.1

There are a large number of rough and irregular fractures in underground engineering rock mass, which makes the movement of fracture fluid very complex. To address the issues of non-visibility of physical model tests and non-repeatability of joint surfaces in natural rock fractures, a method was proposed to accurately depict the impact of rough fracture morphology on rock seepage. This method involved using natural rock fractures as a prototype and creating secondary molds using gypsum and silica gel to precisely replicate the rough fracture surface morphology. The three-dimensional morphology scanning technology was used to accurately obtain the surface morphology information of rough fractures, and the anisotropy of rough fracture morphology parameters was analyzed. Additionally, the study investigated the characteristics of seepage area and frontal flow line morphology through the use of a visual transparent fracture model. The obtained results provided an intuitive understanding of the distribution law of the seepage field. The evolution law of flow velocity with roughness was analyzed by layer superposition technique. The point of maximum flow velocity corresponded to the point of abrupt change in elevation, which occurred in the region with the greatest fluctuation height of the fracture surface. The accurate description of flow patterns in various rough fractures offers theoretical guidance for seepage in rock fractures in practical engineering applications.

Identification of MicroRNAs as potential biomarkers for detecting ischemic stroke

Li Kexin,Shen Li,Zheng Pingping,Wang Yanjun,Wang Lijuan,Meng Xiaoli,Lv Yaogai,Xue Zhiqiang,Guo Xin,Zhang Anning,Pan Pan,Bi Chunli,Chen Yang,Feng Tianyu,Li Bo,Jin Lina,Yao Yan 한국유전학회 2022 Genes & Genomics Vol.44 No.1

Background: Increasing epidemic of ischemic stroke (IS) makes it urgent to understand the pathogenesis and regulatory mechanism, previous studies have described microRNAs (miRNAs) is part of the brain's response to ischemia. Objective: The aim of this study was to screen potential biomarkers for the prediction and novel treatment of IS. Methods: Differentially expressed miRNAs were screened from three newly diagnosed IS patients and three controls by RNA sequencing technology. Furthermore, target prediction databases were then used to analysis the target genes of different expressed miRNAs, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database were used to identify the functions and the main biochemical and signal pathways of differentially expressed target genes. Results: Our results revealed that 27 miRNAs were differentially expressed in IS, among which, hsa-miR-659-5p was the most highly increased and was first found to be associated with IS. In addition, KEGG pathway analyses showed that differentially expressed miRNAs were mainly significantly enriched in lysosome pathway, cytokine-cytokine receptor interaction pathway, spliceosome pathway, base excision repair pathway. Conclusions: miRNAs were involved in IS pathogenesis, and hsa-miR-659-5p, hsa-miR-151a-3p and hsa-miR-29c-5p as the three highest |log2FoldChange| regulation in this study, which may be the biomarkers of IS and need further study.

A biodegradable magnesium alloy sample induced rat osteochondral defect repair through Wnt/β-catenin signaling pathway

Zhao, Kexin,Chen, Yingqi,Yu, Fei,Jian, Weng,Zheng, Ming,Zeng, Hui Techno-Press 2022 Advances in nano research Vol.12 No.3

Many studies have shown that Mg-Nd-Zn-Zr (abbreviated as JDBM) alloy has good biocompatibility and biodegradability as well as promotion of cell adhesion, proliferation and differentiation, and Wnt/β-catenin signaling pathway may play a unique role in joint tissue by controlling the function of chondrocytes, osteoblasts and synoviocytes. However, it is not clear whether the JDBM alloy induces osteochondral repair through Wnt/β-catenin signaling pathway. This study aims to verify that JDBM alloy can repair osteochondral defects in rats, which is realized by Wnt/β-catenin signaling pathway. In this study, the osteochondral defect model of the right femoral condyle non-weight-bearing area in rats was established and randomly divided into three groups: Control group, JDBM alloy implantation group and JDBM alloy implantation combined with signaling pathway inhibitor drug ICRT3 injection. It was found that after JDBM alloy implantation, the bone volume fraction (BVF) became larger, the bone trabeculae were increased, the relative expression of osteogenesis gene Runx2, Bmp2, Opn, Ocn and chondrogenesis gene Collagen II, Aggrecan were increased, and the tissue repair was obvious by HE and Masson staining, which could be inhibited by ICRT3.

Investigation of Microscale Laser Shock Flat Hole Clinching

Yaxuan Hou,Kexin Ding,Guoxin Lu,Chao Zheng,Zhong Ji 한국정밀공학회 2022 International Journal of Precision Engineering and Vol.23 No.9

This paper proposes an improved laser shock flat hole clinching process for joining ductile and brittle materials to obtain single-sided flat joints in microscale. A copper foil is clinched with a perforated sheet and a mechanical joining is achieved by this process. Experiments and numerical simulations are conducted for three thickness combinations of 50 μm/50 μm, 30 μm/50 μm and 20 μm/100 μm to investigate the joinability of this process. The results show that a step-by-step laser shock process with a low-energy pre-shock and a high-energy secondary shock can effectively join the copper foil and the perforated sheet. The three thickness combinations of the upper and lower sheets result in three clinching structures, namely stacked, intermediate and thinning joints. And the joint strength mainly depends on the thinner sheet in a successful joining even for the single joint or the double-joint.

Study on the Construction of a Mechanism to Coordinate Interests in the Exercise of the Right to Transfer Personal Information

Guangjun Zhang(?光君),Kexin Zheng(?可欣) 동아대학교 법학연구소 2022 東亞法學 Vol.- No.97

중국의 개인정보보호법은 처음으로 개인정보양도권을 확립하고 국가인터넷정보통신부가 관련 시행세칙을 제정하도록 허가 하고 있다. 개인정보 이전권은 유럽연합 일반 데이터 보호 규정에서 정한 데이터 이전 권리에 대한 제안이지만 개인정보 이전 권리를 행사하는 과정에서 제3자 플랫폼과 원 플랫폼 간 이해 충돌이 발생할 수 있다. 이익 조정 메커니즘의 구축은 관련 시행 규칙을 제정하는 핵심 과제로 개인정보 이전권 행사의 장벽을 줄이는 데 도움이 되어 개인정보를 잘 활용할 수 있고 동시에 잘 보호할 수 있다. “오늘의 헤드라인” 사례는 개인정보이전에 따른 이해충돌의 복잡성을 잘 보여주고 있으며, 조정메커니즘을 시급히 구축해야 할 필요성이 있음을 잘 보여주고 있다. 개인정보 이전권의 체계적인 권리구조를 구축함으로써 개인정보 이전권이 데이터 휴대권과 다르다는 특수성을 보여준다. 충돌하는 두 플랫폼의 각각의 이익을 분석해 볼 때, 어느 일방에게만 유리하게 보호하는 것은 바람직하지 않다. 각계각층의 다양한 이해관계를 조율할 수 있는 장치가 마련돼야 한다. 그 내용으로 다음과 같은 방안이 제시될 수 있다. 첫째, 비용분담 메커니즘을 구축할 필요가 있다. 제3자 플랫폼은 대가 지급을 통해 기존 플랫폼과 개인정보 이전 비용을 공동 부담한다.원래 플랫폼은 독립적으로 인증 비용을 부담하며 다양한 유형의 개인정보에 따라 다양한 인증방법과 프로세스를 채택한다. 둘째, 의무분배 메커니즘을 수립해야 한다. 기존 플랫폼은 제3자 플랫폼의 컴플라이언스 의무 이행 능력을 심사해 이행 능력이 부족한 제3자 플랫폼에 도움을 줘야 하지만, 일정 기간 후 탈퇴할 수 있는 메커니즘을 설정할 필요가 있다. 전송의무에서 정보이전을 이전단계와 환원단계로 구분하여 의무의 분배. 보안 의무 측면에서 기존 플랫폼이 주요 책임을 지고 제3자 플랫폼이 보조 책임을 지며 보험 가입을 통해 위험을 균등하게 부담한다. China’s Personal Information Protection Law establishes the right to transfer personal information for the first time and authorizes the state Internet information department to formulate relevant implementation rules. Although the right to transfer personal information is a limitation of the right to carry data as stipulated in the EU General Data Protection Regulation(GDPR), the process of exercising the right to transfer personal information still leads to conflict of interests between the third-party platform and the original platform. Constructing a mechanism for coordination of interests is a key task in formulating relevant implementation rules, which is conducive to reducing resistance to the exercise of the right to transfer personal information and realizing the dual purpose of “strong use and protection” of personal information. The introduction of the “Today’s headlines case” highlights the complexity of the conflict of interest in the transfer of personal information and illustrates the urgency of constructing a mechanism for coordination of interests. Then, we systematically construct the right structure of the right to transfer personal information and reveal its special features that distinguish it from the right to carry data. The analysis of the respective interests of the two platforms shows that it is not desirable to adopt a single value orientation for protection, but rather to construct a coordination mechanism for the coexistence of multiple interests. Further, in the process of exercising the right to transfer personal information, it is necessary to balance the interests of the original platform and the third-party platform in terms of cost-sharing mechanism and obligation-sharing mechanism. First, about the cost-sharing mechanism. The third-party platform should share the cost of transferring personal information with the original platform by paying the consideration; the cost of identity verification should be borne by the original platform independently, and the way and process of identity verification should be distinguished according to the type of personal information. Secondly, about the obligation sharing mechanism. The original platform shall review the third-party platform’s ability to fulfill compliance obligations and provide assistance to those with insufficient ability to fulfill obligations, but set up an exit mechanism within a certain period of time; among the transmission obligations, distinguish between transfer and restoration for obligation sharing; among the security obligations, the original platform shall bear the main responsibility while the third-party platform shall bear the auxiliary responsibility, and share the risks among them equally by purchasing insurance.

Dysregulated lncRNA and mRNA may promote the progression of ischemic stroke via immune and inflammatory pathways: results from RNA sequencing and bioinformatics analysis

Wang Yingshuang,Feng Feifei,Zheng Pingping,Wang Lijuan,Wang Yanjun,Lv Yaogai,Shen Li,Li Kexin,Feng Tianyu,Chen Yang,Liu Zhigang,Yao Yan 한국유전학회 2022 Genes & Genomics Vol.44 No.1

Background: Long non-coding RNAs (lncRNAs) are widely involved in gene transcription regulation and which act as epigenetic modifiers in many diseases. Objective: To determine whether lncRNAs are involved in ischemic stroke (IS), we analyzed the expression profile of lncRNAs and mRNAs in IS. Methods: RNA sequencing was performed on the blood of three pairs of IS patients and healthy controls. Differential expression analysis was used to identify differentially expressed lncRNAs (DElncRNAs) and mRNAs (DEmRNAs). Based on the co-expression relationships between lncRNA and mRNA, a series of bioinformatics analysis including GO and KEGG enrichment analysis and PPI analysis, were conducted to predict the function of lncRNA. Results: RNA sequencing produced a total of 5 DElncRNAs and 144 DEmRNAs. Influenza A pathway and Herpes simplex infection pathway were the most significant pathways. EP300 and NFKB1 were the most important target proteins, and Human leucocyte antigen (HLA) family were the key genes in IS. Conclusions: Analysis of this study revealed that dysregulated lncRNAs in IS may lead to IS by affecting the immune and inflammation system.

The Work Experience of Newly Recruited Male Nurses during COVID-19: A Qualitative Study

Huiyue Zhou,Xin Wang,Ruofei Du,Xiang Cheng,Kexin Zheng,Shiqi Dong,Justin Henri,Changying Chen,Tao Wang 한국간호과학회 2021 Asian Nursing Research Vol.15 No.3

Purpose: This study was to investigate the work experience of newly recruited male nurses during the COVID-19 pandemic. Methods: With a phenomenological approach, this qualitative study was adopted semistructured interviews by phone or video calls. A total of 9 male nurses newly recruited for the COVID-19 wards in Chinese hospitals were interviewed for this study. And Colaizzi's method was applied for evaluation in the data analysis. Results: Based on our findings, three themes were extracted. First, the newly recruited male nurses showed negative emotions at the beginning of COVID-19 epidemic, which was caused by changes in working conditions and content, but also prompted the nurses to change the way of coping with the crisis. Second, they gradually mastered the working skills and psychological training to cope with COVID- 19 and developed a positive attitude toward life and a high sense of professional responsibility. Finally, we learned about their needs to respond to public health emergencies such as the COVID-19 pandemic. Conclusion: COVID-19 is a disaster for all of humanity. The newly recruited male nurses are an important force in emergency rescue. Although they suffered from short-term negative emotions, they quickly adapted to the crisis. In order to better prepare for future emergencies, the disaster response capacity of newly recruited male nurses needs to be further improved. In addition, newly recruited male nurses have a strong demand for timely and personalized career development guidance.

A newly characterized exopolysaccharide from Sanghuangporus sanghuang

Xiao-kui Ma,Xu She,Eric Charles Peterson,Yu Zhen Wang,Peng Zheng,Hongyan Ma,Kexin Zhang,Jian Liang 한국미생물학회 2019 The journal of microbiology Vol.57 No.9

Sanghuangporus sanghuang is a well-known pharmacodynamic and economically important edible fungus associated with mulberry (Morus spp.). A distinctly new exopolysaccharide (EPS), designated SHP-2 was obtained from S. sanghuang P0988 broth, and its structure and anti-aging prosperity were characterized. SHP-2 was found to be composed of a backbone of 4)-β-Manp-(1 4)-α-Araf-(1 3,4)-α-Glcp(1 3,4)-α-Glcp-(1 3,4)-α-Glcp-(1 3,4)-α-Glcp-(1 3,4)-α- Glcp-(1 6)-α-Galp-(1 4)-β-Manp-(1 and five branches, including four α-D-Glcp-(1 and one α-D-Manp-(1 SHP-2 was shown to increase antioxidant enzyme activities including catalase (CAT) and superoxide dismutase (SOD) activities, as well as trolox equivalent antioxidant (TEAC) capacity in serum of mice pre-treated with D-Gal, while reducing lipofuscin levels. SHP-2 exerted a favorable influence on immune organ coefficients and ameliorated the histopathological hepatic lesions and apoptosis in hepatocytes of Dgalactose- aged mice almost in a dose-dependent manner. Using the same analytical methods, on comparison with previously studied EPS compounds (i.e. SHP-1), SHP-2 was found to have more complex structure, larger molecule weight, and different anti-aging properties. The results presented here suggest that not only does EPS bioactivity vary with respect to molecular structures and molecule weight, but that multiple structures with different activity can be expressed by a single fungal strain. These results may help understanding the antiaging prosperity of these polysaccharides for use in health foods or dietary supplements.

Genetic variant in TP63 on locus 3q28 is associated with risk of lung adenocarcinoma among never-smoking females in Asia

Hosgood III, H. Dean,Wang, Wen-Chang,Hong, Yun-Chul,Wang, Jiu-Cun,Chen, Kexin,Chang, I-Shou,Chen, Chien-Jen,Lu, Daru,Yin, Zhihua,Wu, Chen,Zheng, Wei,Qian, Biyun,Park, Jae Yong,Kim, Yeul Hong,Chatterje Springer-Verlag 2012 HUMAN GENETICS Vol.131 No.7

<P>A recent genome-wide association study (GWAS) of subjects from Japan and South Korea reported a novel association between the TP63 locus on chromosome 3q28 and risk of lung adenocarcinoma (p = 7.3 10(-12)); however, this association did not achieve genome-wide significance (p 10(-7)) among never-smoking males or females. To determine if this association with lung cancer risk is independent of tobacco use, we genotyped the TP63 SNPs reported by the previous GWAS (rs10937405 and rs4488809) in 3,467 never-smoking female lung cancer cases and 3,787 never-smoking female controls from 10 studies conducted in Taiwan, Mainland China, South Korea, and Singapore. Genetic variation in rs10937405 was associated with risk of lung adenocarcinoma [n = 2,529 cases; p = 7.1 10(-8); allelic risk = 0.80, 95% confidence interval (CI) = 0.74-0.87]. There was also evidence of association with squamous cell carcinoma of the lung (n = 302 cases; p = 0.037; allelic risk = 0.82, 95% CI = 0.67-0.99). Our findings provide strong evidence that genetic variation in TP63 is associated with the risk of lung adenocarcinoma among Asian females in the absence of tobacco smoking.</P>