The CR SPR/Cas9-Generated HEAD NG DATE 3a Mutation Enhances Leaf Production in Perilla frutescens

Sivabalan Karthik(Sivabalan Karthik),Jae Bok Heo(Jae Bok Heo) 한국육종학회 2023 한국육종학회 공동학술발표집 Vol.2023 No.-

Morphological and Mechanical Behaviour of Cu–Sn Alloys—A review

M. Karthik,J. Abhinav,Karthik V. Shankar 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.7

The current paper reviews the research conducted on wrought Cu–Sn, Cu–Sn–Ti and Cu–Sn–Zn alloys intending to revealthe details on morphological and mechanical behaviour during various manufacturing operations. The motivational force ofemploying copper-based alloys in automotive and marine industries is its mechanical properties and corrosion resistance. One of the important factors that afect mechanical properties is micrograph. The grain size, grain distribution, heat treatment, morphological behaviour and the intermediate phase formation highly infuence the properties of the copper-basedalloys. Moreover, the change in microstructure through diferent manufacturing process (wrought process), heat treatmentand addition of alloying element also improve the mechanical and thermal properties. This paper aims to review the majorconclusions on improving the microstructure and mechanical behaviour of wrought Cu–Sn, Cu–Sn–Zn and Cu–Sn–Ti alloys. Analysis and observations of each investigation were prepared by incorporating the morphological results obtained throughOptical Microscope, Transmission Electron Microscope, Scanning Electron Microscope, X-ray Difraction Technique andElectron Dispersive Spectroscopy. This review paper also attempts to give the areas of future researches and developmentsthat can be made for these wrought alloys.

Synthesis and characterization of conductive flexible cellulose carbon nanohorn sheets for human tissue applications

Karthik Paneer Selvam,Taichi Nagahata,Kosuke Kato,Mayuko Koreishi,Toshiyuki Nakamura,Yoshimasa Nakamura,Takeshi Nishikawa,Ayano Satoh,Yasuhiko Hayashi 한국생체재료학회 2020 생체재료학회지 Vol.24 No.4

Background: Conductive sheets of cellulose and carbon nanomaterials and its human skin applications are an interesting research aspect as they have potential for applications for skin compatibility. Hence it is needed to explore the effects and shed light on these applications. Method: To fabricate wearable, portable, flexible, lightweight, inexpensive, and biocompatible composite materials, carbon nanohorns (CNHs) and hydroxyethylcellulose (HEC) were used as precursors to prepare CNH-HEC (Cnh-cel) composite sheets. Cnh-cel sheets were prepared with different loading concentrations of CNHs (10, 20 50,100 mg) in 200 mg cellulose. To fabricate the bio-compatible sheets, a pristine composite of CNHs and HEC was prepared without any pretreatment of the materials. Results: The obtained sheets possess a conductivity of 1.83 × 10− 10 S/m and bio-compatible with human skin. Analysis for skin-compatibility was performed for Cnh-cel sheets by h-CLAT in vitro skin sensitization tests to evaluate the activation of THP-1 cells. It was found that THP-1 cells were not activated by Cnh-cel; hence Cnh-cel is a safe biomaterial for human skin. It was also found that the composite allowed only a maximum loading of 100 mg to retain the consistent geometry of free-standing sheets of < 100 μm thickness. Since CNHs have a unique arrangement of aggregates (dahlia structure), the composite is homogeneous, as verified by transmission electron microscopy (TEM) and, scanning electron microscopy (SEM), and other functional properties investigated by Raman spectroscopy, Fourier transform infrared spectroscopy (FT-IR), conductivity measurement, tensile strength measurement, and skin sensitization. Conclusion: It can be concluded that cellulose and CNHs sheets are conductive and compatible to human skin applications.

The Efficacy of the Novel Bioavailable Curcumin (Cureit) in the Management of Sarcopenia in Healthy Elderly Subjects: A Randomized, Placebo-Controlled, Double-Blind Clinical Study

Karthik Varma,Augustine Amalraj,Chandradhara Divya,Sreeraj Gopi 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.1

Sarcopenia represents the progressive loss of skeletal muscles, which occurs as a result of aging. Plant-derived phytochemicals have the potential ability to manage sarcopenial conditions. The randomized, placebo-controlled, double-blind clinical study involving thirty subjects evaluated the efficacy of Cureit™ supplementation in the management of sarcopenial conditions by measuring the variables, such as hand grip strength, weight lift strength, time/distance before feeling tired after cycling, walking and climbing stairs, and Karnofsky performance scale index along with effects on general fitness, such as protein, urea, oxidative stress, and hematology parameters. The results showed that Cureit supplementation resulted in a significant increase of 1.43% (P < .001) in the handgrip strength compared with placebo. The weight-lifting capacity of subjects supplemented with Cureit showed an increase of 6.08%, whereas placebo showed a 4.54% decrease after the end of the study period. The results demonstrated that the Cureit tended to have a positive impact on distance covered before feeling tired as shown by an increase (P = .09) of 5.51%, compared with placebo group, which showed an increase of 2.29%. The time taken to walk the same distance was reduced in the Cureit group (1.15%), whereas in the placebo group, it was increased (2.02%). Cureit plays a significant role in the management of sarcopenia by anti-inflammatory action, increased hand grip strength, antifatigue effects, and muscle protein management. Clinical Trials Registry-India registration no. CTRI/2018/05/014176

Plasma cell leukemia in North India: retrospective analysis of a distinct clinicohematological entity from a tertiary care center and review of literature

Karthik Bommannan,Man Updesh Singh Sachdeva,Pankaj Malhotra,Narender Kumar,Prashant Sharma,Shano Naseem,Jasmina Ahluwalia,Reena Das,Neelam Varma,Gaurav Prakash,Alka Khadwal,Radhika Srinivasan,Subhash 대한혈액학회 2016 Blood Research Vol.51 No.1

BackgroundPlasma cell leukemia (PCL) is a rare and aggressive plasma cell neoplasm. In PCL, clonal plasma cells comprise ≥20% of the peripheral blood (PB) leukocytes and/or the absolute clonal PB plasma cell count is ≥2×109/L. Primary PCL (PPCL) originates de novo, whereas, secondary PCL (SPCL) evolves from pre-existing multiple myeloma.MethodsClinicohematological features, immunophenotypic profile, and survival of PCL patients were analyzed retrospectively.ResultsBetween January 2007 and December 2014, ten PPCL and four SPCL patients were inves-tigated (8 PPCLs and 3 SPCLs had complete clinical data). All were North Indians, sharing common geography and ethnicity. Our cohort showed less frequent renal failure, more frequent hepatomegaly, and non-secretory type disease. In contrast to western literature, flow cytometric immunophenotyping of our cohort revealed altered expression of CD138 (67%), CD56 (33%), and CD20 (0%). With novel therapeutic agents, these PPCL patients had a median overall survival of 15 months.ConclusionWe highlight that our PPCL patients from North India had distinct clinicohematological and immunophenotypic profiles. The significance of our findings must be tested in a larger patient cohort and must be supported by molecular and cytogenetic investigations to un-mask possible significant effects on pathogenesis.

Hemophagocytic lymphohistiocytosis secondary to histoplasmosis

Karthik Bommanan BK,Shano Naseem,Neelam Varma 대한혈액학회 2017 Blood Research Vol.51 No.2

Electro-synthesis of sulfur doped nickel cobalt layered double hydroxide for electrocatalytic hydrogen evolution reaction and supercapacitor applications

Karthik, Namachivayam,Edison, Thomas Nesakumar Jebakumar Immanuel,Atchudan, Raji,Xiong, Dangsheng,Lee, Yong Rok Elsevier 2019 Journal of electroanalytical chemistry Vol.833 No.-

<P><B>Abstract</B></P> <P>In this work, sulfur doped nickel cobalt layered double hydroxide nanosheet arrays are fabricated on a stainless steel (S-LDH/SS) substrate by simple electrodeposition technique for electrocatalytic hydrogen evolution reaction (HER) and supercapacitor applications. The surface morphology and chemical composition of S-LDH/SS are accessed by field emission electron microscopy (FE-SEM) with energy dispersive X-ray spectroscopy (EDX) and X-ray photoelectron spectroscopy (XPS), respectively. Remarkably, the fabricated S-LDH/SS electrode requires low overpotential of −0.38V vs. RHE to accomplish a current density of −10mAcm<SUP>−2</SUP> with a less Tafel slope of −69mVdec<SUP>−1</SUP> for HER in aqueous KOH. Further, the tailored S-LDH/SS behaves like a battery-type electrode and delivers maximum specific capacity of 344Cg<SUP>−1</SUP> (95.5mAhg<SUP>−1</SUP>) at scan rate of 5mVs<SUP>−1</SUP>, which possess excellent cyclic stability and capacity retention. The S-LDH/SS comprised by two types of active catalytic sites along with anions furnish an excellent conductivity, which improving the mass and ionic transportations. Hopefully, exceptional electrochemical interpretation substantiates that this S-LDH/SS electrode might be a favorable material for the energy conversion and storage applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Sulfur doped nickel cobalt layered double hydroxide nano-arrays were electrodeposited on stainless steel (S-LDH/SS). </LI> <LI> S-LDH/SS displayed good electrochemical performance in KOH. </LI> <LI> S-LDH/SS required low onset and low overpotential for HER. </LI> <LI> S-LDH/SS delivered maximum specific capacity of 344Cg<SUP>−1</SUP> at 5mVs<SUP>−1</SUP>. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Primary adrenal T-cell lymphoma in a young adult presented with pseudo-hypopyon: a case report and literature review

Karthik Bommannan,Man Updesh Singh Sachdeva,Aravind Sekar,Rajender Kumar,Pranab Dey 대한혈액학회 2017 Blood Research Vol.52 No.3

Responsiveness of Commonly Used Patient-Reported Outcome Instruments in Lumbar Microdiscectomy

Karthik Vishwanathan,Ian Braithwaite 대한척추외과학회 2019 Asian Spine Journal Vol.13 No.5

Study Design: Prospective observational cohort study. Purpose: To evaluate the comparative responsiveness of Oswestry Disability Index (ODI, version 2.0), 24-item Roland-Morris Disability Questionnaire (RMDQ), 12-item Short-Form Health Survey (SF-12) physical component score (PCS), and SF-12 mental component score (MCS) in patients that underwent micro-discectomy for lumbar disc herniation. Overview of Literature: Responsiveness is a context specific term and no study has reported the responsiveness of ODI version 2.0, RMDQ, SF-12 PCS, or SF-12 MCS in discectomy patients. Methods: Responsiveness was assessed using distribution-based methods (effect size [ES], standardized response means [SRM], SRM difference between patients who improved and those who did not show improvement [SRM difference]), and the anchor-based method (area under the curve [AUC] of receiver operating characteristic curves). Values of ES and SRM higher than 0.8, and AUC value higher than 0.7 suggest adequate responsiveness. Outcome instrument demonstrating the highest value of SRM difference and AUC was considered the most responsive. Results: Responsiveness was assessed in 98 participants at a mean follow-up time of 12 weeks postoperatively. The overall ES of RMDQ, ODI, SF-12 PCS, and SF-12 MCS was 2.15, 2.11, 2.08, and 0.86, respectively. The overall SRM of ODI, RMDQ, SF-12 PCS, and SF-12 MCS was 1.36, 1.43, 1.24, and 0.65, respectively. The SRM difference in RMDQ, ODI, SF-12 PCS, and SF-12 MCS was 2.64, 2.26, 1.32, and 1.29, respectively. The AUC of ODI, RMDQ, SF-12 PCS, and SF-12 MCS was 0.96, 0.96, 0.83, and 0.83, respectively. Conclusions: ODI, RMDQ, SF-12 PCS, and SF-12 MCS demonstrated adequate responsiveness in a homogenous cohort of patients who underwent lumbar micro-discectomy. ODI and RMDQ are equally responsive and, thus, are interchangeable instruments for region specific outcomes. Both the SF-12 PCS and SF-12 MCS can assess the quality of life following lumbar micro-discectomy.

Evolving live load criteria in bridge design code guidelines - A case study of India based on IRC 6

Karthik, P.,Sharma, Shashi Kant,Akbar, M. Abdul Techno-Press 2022 Structural monitoring and maintenance Vol.9 No.1

One of the instances which demand structural engineer's greatest attention and upgradation is the changing live load requirement in bridge design code. The challenge increases in developing countries as the pace of infrastructural growth is being catered by the respective country codes with bigger and heavier vehicles to be considered in the design. This paper presents the case study of India where Indian Roads Congress (IRC) codes in its revised version from 2014 to 2017 introduced massive Special vehicle (SV) around 40 m long and weighing 3850 kN to be considered in the design of road bridges. The code does not specify the minimum distance between successive special vehicles unlike other loading classes and hence the consequences of it form the motivation for this study. The effect of SV in comparison with Class 70R, Class AA, Class A, and Class B loading is studied based on the maximum bending moment with moving load applied in Autodesk Robot Structural Analysis. The spans considered in the analysis varied from 10 m to 1991 m corresponding to the span of Akashi Kaikyo Bridge (longest bridge span in the world). A total of 182 analyses for 7 types of vehicles (class B, class A, class 70R tracked, class 70R wheeled, class AA tracked, AA wheeled, and Special vehicle) on 26 different span lengths is carried out. The span corresponding to other vehicles which would equal the bending moment of a single SV is presented along with a comparison relative to Standard Uniformly Distributed Load. Further, the results are presented by introducing a new parameter named Intensity Factor which is proven to relate the effect of axle spacing of vehicle on the normalized bending moment developed.