Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

New Insight for Fluoroquinophenoxazine Derivatives as Possibly New Potent Topoisomerase Ⅰ Inhibitor

Kang, Da-Hye,Kim, Jung-Sook,Jung, Mi-Ja,Lee, Eung-Seok,Jahng, Yurngdong,Kwon, Youngjoo,Na, Younghwa 이화여자대학교 약학연구소 2008 藥學硏究論文集 Vol.- No.18

Fluoroquinolones, represented by ciproxacin and norfloxacin, are well known clinical antimicrobial agents, and their phenyl ring expanded quinophenoxazines are reported as possible antitumor active compounds. These quinophenoxazines are known to inhibit DNA topoisomerase II essential for cell replication cycle. But there were no reports for topoisomerase I inhibition study for these compounds. In this report, we have prepared a few quinophenoxazine analogues and tested their topoisomerases I and II inhibitory activities and cytotoxicity. From the result, we found that qumophenoxazine analogues possessed strong topoisomerase I inhibitory capacity as well as topoisomerase II inhibition. Among the compounds prepared, A-62176 analogues showed strong topoisomerases I and II inhibitory activities. Interestingly, compound 8 missing the 3-aminopyrrolidine moiety at C2 position has similar potent inhibitory capacity against topoisomerases I & II at higher concentrations (20 and 10 uM, respectively). But compound 8 inhibited topoisomerase I function more selectively at lower concentration, 2 uM. Our observation mi&ht strongly implicate that fluoroquinophenoxazines can be developed as efficient topoisomerase I inhibitor with the elaborate modification.

유아 로타바이러스 설사증에 대한 IgY 난황 항체의 효과시험

정동혁, 박효선, 김경미, 정규항, 김강영, 정후길, 윤숭섭, 전호남, 강신영 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.2

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언어 영재 교육을 위한 프로젝트 학습 프로그램 개발 연구

강미정(Kang Mi-jung) 한국어문교육학회 2008 어문학교육 Vol.36 No.-

  The purpose of this study is to construct and suggest project learning program suitable for the verbally gifted by searching its possibility as a method of learning program which enhances creative problem-solving ability of the verbally gifted.<BR>  To achieve the purpose of this study, the conception of the verbally gifted is established. The verbally gifted are defined as students who acquire language in their early life or are prominent in language domain(or have such possibility) with usually high lingual comprehension, production ability and lingual habits.<BR>  Learning program for the verbally gifted is mostly progressed with in-depth study, especially project learning program is shown as one of in-depth study programs which considerably correspond with cognitive and affective characteristics of the verbally gifted.<BR>  To construct actual project learning, I first selected learning themes, that is, content factors which can expect the educational effect of project learning among the educational contents for the verbally gifted. I analysed the former researches such as Choi, Jong-Oh(2004), Park Su-Ja(2004), Lee Sun-Young(2000), Lee Mi-Kyung(2005), Kwon Sun-hee(2006) et al, creative writing texts for the verbally gifted of educational districts in Busan, texts related to the verbally gifted published in KEDI and real teaching contents for the verbally gifted. As a result, four learning themes are selected; "Writing creatively", "Making ads.", "Researching authors", and "Dramatic role play".<BR>  To present selected themes to the verbally gifted easily, I made them concrete by the name of the following project; "Come here, Poet", "My own ads" "Searching for the authors", and "on the spot at that time". Such project themes make process project learning program through 5 steps; Finding out theme→ Planning project → Implementing project → Creating products → Presentation?exhibition & evaluation. This project learning program means somewhat different from the existing project learning programs. Considering cognitive and affective characteristics of the verbally gifted, it focuses on enhancing their creative problem-solving ability as much as possible.<BR>  Now gifted education of Korea is in the midst of transition periods. Besides visibly splendid results. there are many unexpected problems. That"s why deeper research should be conducted in various areas including the verbally gifted.<BR>  Finally, I want to raise interest towards the verbally gifted through this study and provide properly educational opportunities for them. I hope this study can be a tuning point which is of help to teachers in charge of gifted education.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

Hepatic Cirrhosis Occurring in a Young Woodchuck(Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus(WHV)

Kyu-shik Jeong,정원일,Jae-yong Chung,Mi-young An,Chae-yong Jung,Gyoung-jae Lee,Jong-soo Kang,Byeong-cheol Kang,Young-heun Jee,Bruce H Williams,Young-oh Kwon,Da-hee Jeong 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Cirrhosis Occurring in a Young Woodchuck (Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus (WHV)Da-hee Jeong, Won-il Jeong, Jae-yong Chung, Mi-young An, Chae-yong Jung, Gyoung-jae Lee1, Jong-soo Kang1, Byeong-cheol Kang2, Young-heun Jee3, Bruce H Williams4, Young-oh Kwon5 and Kyu-shik Jeong*College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea1Shinwon Scientific Co., LTD, Research Institute, Suwon, Korea2Clinical Research Institute, Seoul National University, Seoul 110-744, Korea3College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea

비결핵성 마이코박테리아 폐질환의 임상경과

최강현, 한혜숙, 정혜원, 안진영, 이기만, 김미경 충북대학교 의과대학 충북대학교 의학연구소 2011 忠北醫大學術誌 Vol.21 No.2

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윤상 다륜형 아급성 피부 홍반성 루푸스 1예

강민정,박혜진,조소연,이미애,황규광,함정희 대한피부과학회 1999 대한피부과학회지 Vol.37 No.6

We report an annular type of subacute cutaneous lupus erythematosus(SCLE) which is rare in Korea, A 23-year-old female had erythematous annular rashes with a mild itching sensation on the face, trunk and both extremities. She also had painless oral mucosal ulceration, diffuse hair loss and mild arthralgia. Serologic abnormalities of the patient were positive anti-Ro Ab, ANA and Anti-ds-DNA Ab. The histopathologic findings showed vacuolar degenerations in the basal cell layer, mononuclear cell infiltrate in the upper dermis, and dermal edema. Direct IF revealed granular deposition of IgG and IgA at the dermo-epidennal junction.

피드백을 이용한 문제은행 시스템의 설계 및 구현

강신재,김정미 대구대학교 2005 대구대학교 학술논문집 Vol.1 No.1

현재 웹상에는 많은 온라인 문제은행이 있고 또 생겨나고 있다. 이러한 문제은행들은 회원이나 학습자의 실력향샹을 도모하기 위하여 피드백을 사용한다. 그러나 아직 피드백의 연구가 활발히 이루어지지 않고 있어 그 가치를 십분 이용하지 못하고 단순히 반복 학습의 형태로만 사용되어지고 있다. 이에 본 논문에서는 피드백의 효용가치를 재고찰하고 이를 발전시켜 기존 문제 은행시스템의 진보를 꾀하고자 한다. 따라서 본 논문에서는 기존 피드백보다 좀 더 진보된 형태의 피드백 시스템의 형태로 세분화된 피드백을 제안하였다. 개인피드백과 전체피드백으로 세분화 하고 단순 반복의 학습형태를 탈피하여 문제 출제시 피드백의 일정부분을 재출제하는 형태로 그 방향을 제시하고 있다. 그리고 이 시스템이 기존 시스템보다 효율적인 것을 규명하였다. There are many on-line problem banks on the web and they are still be created. These problem banks put 'feedback' to practical use. However, we simply use 'feedback' to repeat studying without applying its whole value because the research on 'feedback' can't be accomplished vigorously. Then, the paper shall make an attempt to re-consider utility and value of 'feedback', and progress the existing system of problem banks. Therefore, the paper proposed a much advanced, detailed 'feedback' system than the existing one. The paper proposed an advanced direction of feedback system which is progressed by forms of representing some problems returning from first feedback. Proposed system is segmented into personal-feedback and whole-feedback, and is broken from simple repeating study form.

Capsaicin에 의한 간암세포주의 Apoptosis 유도

정미영,강혜정,문애리 德成女子大學校 藥學硏究所 2000 藥學論文誌 Vol.11 No.1

Hepatocellular carcinoma is one of the most lethal malignancies and there is no effective preventive measure in this highly malignant disease to date. In the present study, we investigated the chemopreventive potential of capsaicin (8-methyl-N-vanillyl-6-nonenamide), the principal pungent ingredient found in hot red pepper, in SK-Hep-1 hepatocellular carcinoma cells. Treatment of capsaicin inhibited growth of SK-Hep-1 cells in a concentration-dependent manner. The inhibitory effect of capsaicin on SK-Hep-1 cell growth was mainly due to the induction of apoptosis as evidenced by DNA fragmentation and nuclear condensation. Furthermore, capsaicin prominently reduced the ratio of anti-apoptotic Bcl-2 to proapoptotic Bax. We also show that the activity of DEVDase (caspase-3 like) is more likely involved than that of YVADase (caspase-1 like) in capsaicin-induced growth inhibition. These results deminstrate that capsaicin efficiently induced apoptosis in SK-Hep-1 cells through a caspase-3-dependent mechanism, which may contribute to the chemopreventive function for hepatocellular carcinoma.