김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

방역작업자의 브롬화메틸(methyl bromide) 노출수준과 생물학적 모니터링

이종성,이용학,신재훈,최정근,오차재,정호근 한국산업위생학회 2001 한국산업보건학회지 Vol.11 No.3

This study was performed to estimate environmental and biological monitoring of worker exposed to methyl bromide through quarantine fumigation. Airborne methyl bromide and it's metabolites were analyzed by gas chromatography and ion chromatography, respectively. The results are as followings; airborne concentration of methyl bromide(TWA) was 2.08±1.56 ppm(N=8). Dispersion and setting/degas groups were 0.67±0.12 ppm(N=2) and 2.54±1.53ppm(N=6), respectively. Bromide ion concentration in serum was 23.40±14.91㎎/ℓ(N=10) in the exposed workers and 4.74±0.82㎎/ℓ(N=21) in the non-exposed workers. Bromide ion concentration in urine was 35.56±26.89㎎/ℓ(N=11) in exposed group and 6.62±2.31㎎/ℓ(N=21) in non-exposed group. Good correlation was observed between concentration of serum and urine(r^2=0.890 p<0.01). No significant correlations of other determinanats were observed. Calcuated from a regressive curve. the biological half lifes of serum and urine were 10.7 and 5.9 days. In these results, biological monitoring of bromide ion of serum and urine provided useful information for evaluating exposure of workers to methyl bromide, so that an availability of bromide ion of biological samples was showed as biological monitoring indices for methyl bromide.

농산물중의 잔류농약 실태에 관한 연구(Ⅰ) : 고체상 추출에 의한 농산물중 잔류농약 분석에 관한 연구 Analysis of Residual Pesticides in Agricultural Products by Solid-Phase Extraction

허옥순,이재관,이정성,서정혁,주인선,허수정,김세은,김정근,신영희,유미자,김지연,심규창,김성환 식품의약품안전청 1998 식품의약품안전청 연보 Vol.2 No.-

신속 · 정밀하고 효율적인 짠류농약 검출을 위하여 극싱칼럼 (Extrelut-3')을 사옹한 SPE법을 시도하였다. 시료는 채소류체 사용하는 농약중 잔류성이 있고 식품공전상 시험 전처리가 각각 다른 Chlorothalonil등 6종의 농약을 선정하고 그들 표준액에 대한 PPIR수준의 농도로추출용라의 종류 및 용매량 등의 추출조건을 달리하여 회수율 등을 측정한 결과 최적의 분석조건을 얻었다. 1) 추출 용매량은 증가할수록 추출 수율이 높았고,용매 종류에 대한 추출수율은 각 농약별로 다소 차이는 있었으나 극성 정도가 비교적 높은 Ethyl acetate애서 평균 추출수율이 가장 높았다 2) 최대 평균회수율은 Ethyl acetate 60mL를 사웅할 경우로서 93.6%였다. 3)표준액을 시료에 첨가한 시험에서도 역시 Ethyl ace늘to가 푼출수율이 가장 높았으며 불순물 분리제거 효과도 LLE법 보다 월등히 우수하였다. 4) 각 농약별 검출한계는 치소 0.001ppin으로 농산물 중 미량 잔류하는 농약을 검출할 수 있을 것으로 사료된파. 그러므로 SPE법은 LLE법보다 경제적인 방법으로 판명되었으며, 앞으로 농산물 중 잔류농약 분석에서 LEE법을 대치할 쑤 있을 것으로 기대된다. Solid-phase extraction by polar column(Extrelut-3') was attempted to develope the fast and efficient method of detecting pesticide of farm product.5·ix kinds of pesticide used in farming fieldfrequently and different in pretreatment, were chosen from Korea Food Cord. Optfmal analysis condi-tions were determiBed from t31e recovery rate of standard pesticides according to extraction solvent andextraction volurae. Extraction yietd was increased as solvent polarity and extraction volume. Maximumrecovery rate was acquired at 93.6% when 60mL of ethyl acetate was used as eluent. Tllis method wasmore effective than liquid-liquid partition extract·ion method to eliminated the impurity and had 0.001ppm of detection limit. Therefore. solid-phase eEl=raction was expected to be economical substitute fortraditional liquid-liquid extT·action method.

중독한 추체로 증상을 보인 cyclosporine에 의한 뇌증 1예

강정현,곽승근,신현영,김성은,이정호,이정찬,곽상혁,윤환중,조덕연,김삼용,김제,송창준 충남대학교 의과대학 지역사회의학연구소 2000 충남의대잡지 Vol.27 No.1

The use of cyclosporine(CsA), a potent immunosuppressive agent, is associated with numerous side effects. Neurotoxicity was earlier less well known, but with growing experience central nervous system side effects are now reported up to 40% of patients. Among various CsA-induced neurotoxicities, tremor is most common and posterior leukoencephalopathy is well characterized. An encephalopathy presenting with pyramidal weakness, however, seems to be rare and poorly documented. We report here a case of encephalopathy presenting with multiple generalized seizures followed by fluctuating quadriparesis developed a few days after starting CsA and resolved spontaneously after withdrawal of the drug . A 48-year-old woman with aplastic anemia was placed upon immmunosuppressive treatment consisting of antithymocyte globulin(ATG), CsA, and methylprednisolone. Two days after starting the therapy, she developed a generalized seizure. The next day she developed 2 more episodes of seizure and the therapy was stopped. Computed tomogram of the brain was normal. Six days after starting therapy, she developed mild motor weakness and paresthesia of both lower extremities, which progressed to a definite quariparesis confining her to a bed in 3 days. Magnetic resonance imaging(MRI) of the brain revealed asymmetric, bilateral high-signal abnormalites in the subcortical white matter of fronto-temporal areas. After reaching to a maximum with some fluactuation(14 days after starting the therapy) the motor weakness started to be resolved. She was discharged with a almost complete resolution of the weakness 45 days after starting the therapy. Five months later she did well and brain MRI was negative.

유용성 도료와 수용성 도료의 유해성 비교에 관한 연구 : 자동차 보수용 도료를 중심으로

권은혜,김광식,오정룡,최정근,정윤석,이유진,김은아,송세욱,정호근 한국산업위생학회 2001 한국산업보건학회지 Vol.11 No.1

The purpose of this study is to substitute water-based painting materials for the current solvent-based ones used in motor-repairing process to minimize the exposure of organic solvents to the painters. This study assessed the exposure of organic solvents to the painters using water-based and solvent-based painting materials and compared compositions, painting processes and the health hazards of the application of these alternative painting materials. The results of this study are as follows. 1. solvent-based painting materials used in motor-repairing process consist of various organic solvents, which consist primarily of toluene, xylene, ethyl benzene, ethyl methyl bezene, trimethyl bezene, ethyl acetate, butyl acetate, methyl isobutyl ketone, 2-ethoxy ethanol, 2-ethoxy ethyl acetate and toluene-2.4-diisocianate and the others. These organic solvents are know as health-hazardous substances. But water-based painting materials are high-solid, low-solvent ones and consist of such two organic solvents as 2-butoxy ethanol and 2-heptanone and the others. 2. The painters using solvent-based painting materials in motor-repairing process are exposed to various organic acetate, methyl isobutyl ketone, trimethyl benzene, 2-ethoxy ethanol, and 2-ethoxy ethyl acetate. But the painters using solvent-based ones are only exposed to 2-butoxy ethanol and 2-heptanone. 3. By using water-based painting materials in stead of solvent-based painting materials containing health-hazardous organic solvents, the exposure of such organic solvents in the painter's breathing zone can be largely prevented. 4. This study recommends water-based painting materials as substitutes for the current solvent-based ones used in motor-repairing process to minimize the exposure of organic solvents to the painters.

微量의 一酸化炭素 定量에 관한 硏究

李正根,金昇煥 건국대학교 부설 산업기술연구소 1976 논문집 Vol.2 No.-

The gas-chromatographic method and nondispersive infrared spectrophotometric method for the determination of trace amount of carbon monoxide were compared to investigate the degree of precision and accuracy. The gas-chromatographic method combining the hydrogenating and separating column (Chromosorb polymer 104 and molecular sieve 5A)with flame ionization detector was found efficient for the determination of carbon monoxide even under 10ppm. The nondispersive infrared spectrophotometric method whose flow rate is 700c.c./min. and thickness of cell is 128mm. shows the excellent accuracy in determining carbon monoxide less than 10 ppm. However, it could detect the concentration slightly more than the equivalent concentration of the standard carbon monoxide in the range of 150-180ppm.

비보강 조적벽체 전단내력산정에 관한 연구

이봉근,이정한,이원호,오상훈,나정민 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

In Korea, more than four hundred earthquakes with medium intensity centered on off-coastal and inland of Korea during the past 20 years and due to the great earthquakes which occurred recently in neighboring countries the importance of the future earthquake preparedness measures in Korea is highly recognized. More than 80 percents of residential buildings are constructed with unreinforced masonry structure, in Seoul. In general, unreinforced masonry structure don't have enough strength against the lateral force. And low rise buildings such as unreinforced masonry structure didn't adopted seismic design. So, a huge damage is expected when an earthquake occurs. It is that the major variables of shear strength are the aspect ratio, the vertical strength and the method of masonry. The objectives of this study are, 1) to find out the shear behavior of masonry wall and the variables affect the shear capacity of masonry wall such as aspect ratio of the unreinforced masonry wall, and the vertical strength, 2) to propose the equation of the shear capacity of masonry wall by regression analysis using the test data.

자외선 등의 자극에 의한 인체 멜라닌세포의 타이로신 프로테인 키나제 수용체 발현에 관한 연구

이정열,황규왕,이종석,김영근 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.1

Human melanocytes express the p 75 nerve growth factor(NGF) receptors and tyrosine protein kinase receptors(trk) in vitro. Melanocytes are induced to express the p75 NGF receptor in vitro by a variety of phamacologic and physiologic stimuli including UV light. Melanocytes dendricity and migration are regulated in part by NGF. Cultured human epidermal keratino- cytes produce NGF. The purpose of the study was to investigate the influence of UVB and TPA(phorbol 12-tetradecanoate 13-acetate) stimuli in pure melanocytes group and the influence of UVB stimuli in keratinocytes/melanocytes coculture group in the expression of trk A, respectively. Normal human keratinocytes and melanocytes were established from neonatal foreskin. Keratinocytes and melanocytes were cultured by modified Medium 154 and F-10, respectively. Pure melanocytes group and keratinocytes/melanocytes coculture group were respectively exposed to UVB 50 mJ/㎠. pure melanocytes group were treated for 1 day with TPA 50, 100 ng/ml. the expression of trk A in melanocytes with 50 ng/ml TPA was induced about 3 times higher than unstimulated control. But. TPA induction of trk A in melanocytes is not dose-dependent. The expression of trk A in pure melanocytes group and melanocytes/keratinocytes cocultures group with UVB 50 mJ/㎠ was induced about 4-5 times than unstimulated control. We conclude that stimulation with TPA and UVB induces trk A in melanocytes and UVB effect is further potentiated by the presence of keratinocytes in culture.

서중조건하에서의 콘크리트 충전 강관 기둥의 시공을 위한 실험적 연구

이장환,강용학,정근호,김진호,최문식,정상진 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

The basic Physical properties, Slump, Slump flow, Air content, Bleeding, and Settlement of concrete was investigated to test Characteristic of Setting and to evaluate the relation between Model specimen and Heat of hydration for construction Over the High Temperature(CFT). The objective of this study is to take the partial core after the cementation of Model specimen, test the compression intensity and analyze the relation to Test piece.

지방이식의 다양한 임상적 이용

이근철,정영하,김정태,김석권 대한미용성형외과학회 1997 Archives of Aesthetic Plastic Surgery Vol.3 No.1

Generally alloplastic matrials such as collagen compound and liquid silicone injection. or autologous fat injection have been used for correction of surface irregularity. But, except for autologous fat injection, the other methods have been proved to be not effective or even dangerous. Then with the explosion in the popularity of liposuction, the autologous fat injection has been applied to variable clinical uses for contouring of the soft tissue irregularities. The author performed main operations with fat injection as ancillary procedure in 16 patients from 1993 to 1996, and long term follow-ups were reported. The lesions treated most frequently were cheeks, and others were thighs, legs, nasolabial folds and upper eyelids in order of frequency. The donor sites were lower abdomen, thigh, buttock and cheek. Our method of fat injection was used as an ancillary procedure for improving post-operative results in variable cases (craniofacial microsomia, fibrous dysplasia, asymmetry of leg, etc). And the important technical points in our method for reducing the absorption of fat after injection were atraumatic liposuction, multilayered dispersed injection, and 20% overcorrection. We obtained satisfactory aethetic results without major complications.