The expression patterns of RANKL and OPG in murine tooth eruption

Hwang, Kyung-Mun,Kim, Eun-Jung,Kim, Hyun-Jung,,Kim, Young-Jin,Nam, Soon-Hyeun 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.2

치아의 맹출은 치아기 (dental organ)와 치조골의 세포와 연관된 매우 복잡한 과정이다. 우선 치아 맹출이 일어나기 전에 파골세포가 치낭으로 집결하게 된다. 이러한 치낭의 역할은 파골세포와 조골세포의 상호작용으로 이루어 지는 골개조와 밀접한 관련이 있는데, 이는 치아 맹출과 연관된 많은 유전자들이 치낭에서 발현되기 때문이다. RANKL는 TNF ligand family로써 조골세포에 존재하며 파골세포의 형성 및 전구세포로 부터의 활성화를 유도한다. 이러한 RANKL은 OPG에 의해 그 작용이 억제되며 RANKL와 OPG의 상대적인 비율이 파골세포의 형성에 영향을 미친다. 또한 Runx2유전자의 변이는 조골세포의 분화와 활성에 차질을 가져오고 결국 RANKL/OPG pathway를 통해 파골세포 형성에 영향을 줄 수 있다. 치아의 발육 및 맹출에 미치는 RANKL 및 OPG의 영향을 알아보고 Runx2와의 연관성을 알아보기 위해 in situ hybridization 방법으로 태생 1, 3, 5, 7, 9, 11일된 쥐의 하악 및 제1대구치를 사용하여 실험을 실시한 결과 RANKL, OPG, Runx2의 mRNA가 태생 1일부터 11일까지 치낭 및 치아주위조직에 특성있게 나타났다. 이중 태생 5일에서 9일 사이에 RANKL 및 Runx2는 치아의 교합면측과 하방 치조골 부위의 발현이 강하게 나타난 반면 OPG는 약한 발현을 보였다. 이는 또한 파골세포의 활성부위를 알아보기 위해 TRAP염색을 실시하여 태생 5일에서 9일 사이에 최대의 활성화를 나타낸 결과와 연광성 있게 나타났다. RANKL, OPG, Runx2의 특성있는 발현양상들을 종합해 볼 때, 치아 맹출은 치낭, 치아기, 치조골 사이의 상호 작용을 통해 이루어 지며, 이는 치낭이 치아 맹출에 있어서 매우 중요하다는 것을 의미한다. 또한, 이러한 유전자들 (RANKL, OPG, Runx2) 이 치아의 맹출에 중요한 역할을 하는 것으로 사료된다. Tooth eruption is a complex and tightly regulated process that involves cells of the tooth organ and the surrounding alveolus. Osteoclast precursors must be recruited into the dental follicle prior to the onset of eruption. This function of dental follicle may be regarded as the ability of bone remodeling characterized by the interaction of osteoclasts and osteoblasts. This is because tooth eruption is a localized event in which many of the genes required for eruption are expressed in the dental follicle. RANKL is a membrane-bound protein that is a member of the TNF ligand family, which is present on bone marrow stromal cells and osteoblasts, and induces osteoclast formation and activation from precursor cell. The biologic effect of RANKL is inhibited by OPG and, in bone, the relative ratio of RANKL and OPG modulates osteoclastogenesis. To evaluate the roles of RANKL and OPG in tooth eruption and the relations with the expression pattern of Runx2, in situ hybridization was performed with mandibles of mice at postnatal stage 1, 3, 5, 7, 9 and 11. mRNA of RANKL, OPG, and Runx2 are expressed in dental follicle and surrounding tissue from P1 to 11. To determine the sites of osteoclastic activity duing tooth eruption, mandibles were dissected. Peak osteoclastic activity in alveolar bone along the occlusal and basal regions was observed from P5 to 9, with osteoclasts in these regions being large and strongly TRAP-positive. The specific spatic-temporal expression patterns of RANKL, OPG and Runx2 in our study suggest that tooth eruption could be progressed through the interactions of molecular signaling among dental follicle, dental organ and alveolar bone, furthermore it means that dental follicle is quite important in tooth eruption. In addition, it indicates that these genes (RANKL, OPG, and Runx2) play critical roles in tooth eruption.

축구선수의 Detraining과 Retraining이 혈청지질 및 호르몬농도에 미치는 영향

정정화,박재현,채종훈,성혜련,황지인,윤미숙,노금선,윤종관,윤영학,노순덕,정경숙,박일규,김은희,박현태,박상갑 대한스포츠의학회 1999 대한스포츠의학회지 Vol.17 No.1

The purpose of this study was to investigate the effects of detraining and retraining on serum lipid and hormones in soccer players. Subjects were seven male high-school soccer players. V˙O_2max was determined for each subjects by administering a treadmill test(initial speed: 90m/min, grade: 5%, increasing speed per 3 min: 30m/min). Serum lipid(T-C, TG, HDL-C LDL-C) and hormones(epinephrine, norepinephrine, growth hormones, cortisol) were assayed pre and post detraining in 10, 20, 30 days after retraining. The repeated ANOVA was used to determine significant differences. The 0.05 level of significance was as critical level for the study. The results of the study were as follows: 1. V˙O_2max(ml/min) were 3576.3±204.2ml/min pre detraining, 3234.1±198.9 ml/min post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/min) were 3601.4±170.9 ml/min. There were significantly(p<.05) increased in retraining periods. 2. V˙O_2max(ml/kg/min) were significantly(p<.05) decreased from 62.3±2.9 ml/kg/min to 55.9±4.7 ml/kg/min in detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/kg/min) were 62.4±3.4ml/kg/min, 62.7±2.3ml.kg/min, 67.3±7.2ml/kg/min respectively. There were significantly(p<.05) increased in retraining periods. 3. T-C were significantly (p<.05) increased from 166.6±8.5mg/dl to 175.3±10.3 mg/dl in detraining. In 10, 20, 30 days after retraining, T-C were 160.1± 3.2mg/dl, 156.7±3.7mg/dl, 140.3±9.0mg/dl. There were significantly(p<.05) decreased in retraining periods. 4. HDL-C were 61.4±6.6mg/di pre detraining, 5.3±6.6mg/dl post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, HDL-C were 56.9±7.1mg/dl, 56.4±9.2mg/dl, 57.7±9.1mg/dl respectively. There were no significant difference in retraining periods. 5. The hormones(epinephrine. norepinephrine, growth hormone, cortisol) were changed as same patterns. Epinephrine were 26.0±7.0[g/ml pre detraining, 24.6±3.2pg/ml post detraining. In 10, 20, 30 days after retraining, epinephrine were 26.9±5.6pg/ml, 30.6±6.2pg/ml, 29.4±5.6pg/ml respectively. There were no significant difference in retraining periods. In conclusion, HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were decreased, T-C, LDL-C and TG were increased in detraining. But HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were increased, T-C, LDL-C and TG were decreased in retraining.

쌍발 복합재 비행기의 속도, 고도 보정 및 상승성능에 관한 연구

황명신,박윤진,이정모,김칠영,은희봉 한국항공운항학회 1997 한국항공운항학회지 Vol.5 No.1

Airspeed and altimeter calibration of "win Mee was conducted by the flight test. We have adopted system to system method. Flight test data is corrected for instrumented error and position error, and the resultin data was satisfied. Climb Performance flight test also was conducted. But we could not have all data because of limited flight time. The resulted data was satisfied compare with calculated dab.

장내의 탄수화물과 지방 흡수 억제를 통한 체지방 및 비만 개선 효과에 관한 연구

정은희,윤승원,이홍석,윤유식,유경미,황인경 한국조리과학회 2003 한국식품조리과학회지 Vol.19 No.1

In a previous study, a dietary supplement was developed in our lab using natural herbal extracts against digest enzyme activity in GI tract for weight control. This natural herbal extracts could regulate absorption of glucose and lipid by the inhibition of digest enzyme activity. In this study, we screened the natural herbs that inhibit glucoamylase activity and developed an water extract of cinnamon. The cinnamon extract delayed and decreased the increment of carbohydrate degradation through the inhibition of glucoamylase activity in vitro. Fifty volunteers were subjected to the intake of the herbal extracts by taking twice a day for 60 days. As a result, the treated subjects lost 3kg of body weight and 3.5kg of body fat mass after the treatment. Furthermore, the body mass index and waist size were significantly decreased during the experimental period. Above results suggested that the administration of the dietary additives composed of cinnamon and natural herbal extract improves the obesity by the decrement of body weight and body fat mass.

예비 초등교사와 예비 특수초등교사의 과학교수에 대한 태도 연구

황순영,정은영,이미아 국립특수교육원 2006 특수교육연구 Vol.13 No.2

본 연구는 예비 특수초등교사와 예비 초등교사의 과학교수에 대한 태도를 살펴보기 위하여 부산, 경남에 있는 교육대학과 특수교육과예 재학중인 3, 4학년 학생들을 대상으로 하였다. 예비교사의 과학교수에 대한태도를 측정하기 위해 Thompson과 Shrigley()985)가 개발한 과학교수에 대한 태도척도(Revised Science Attitude Scale)를 예비 특수초등교사와 예비 초등교사에 맞게 수정하여 사용하였다. 연구문제에 따라 기술통계와 t-검증, 이원변량분석을 실시하였다. 연구결과를 정리해 보면 첫째, 예비 특수초등교사와 예비 초등교사의 과학교수에 대한 전체 태도에서는 통계적으로 유의미한 차이가 없는 것으로 나타났다. 그러나 예비 특수초등교사가 예비 초등교사들보다 과학교육의 필요성을 보다 높게 지각하고 있는 것으로 나타났다. 둘째, 과학관련 과목 수강 여부에 따른 예비교사의 전체 태도는 유의미한 차이가 없는 것으로 나타났으나, 하위영역 중. 과학교수에 대한 편안함 영역에서는 과학관련 교과목을 수강한 경험이 있는 예비 교사가 더 긍정적인 태도를 보였으며, 필요성 영역에서는 오히려 수강한 경험이 없는 예비 교사가 더 긍정적인 태도를 나타냈다 셋째, 예비 초등교사와 예비 특수초등교사의 과학교수 태도에 대한 문항별 차이를 살펴본 결과, 예비 초등교사는 예비 특수초등교사에 비해 학생들의 질문에 대한 두려움이 크며, 예비 특수초등교사는 다른 교과와 비교해 과학의 중요성에 대해 더 긍정적인 태도를 가지고 있었다. The purpose of this study was to investigate attitude toward science teaching between elementary preservice teacher and special elementary preservice teacher in 3 and 4 grade students of university. For this purpose, the Revised Science Attitude Scale developed by Thompson & Shrigley(1986) was used and the method, t-test, two way ANOVA were used to investig science teach ing attitude for them. The results are as followed : First of all, the reliability if measurement instrument was verified through a pre-study. For the first research question was used. And Discriminative Analysis was used to investigate the second research question. The results of this study were as follows: First, according to the result of t-test, there weren't significant difference of science teaching attitude between two groups. But special elementary preservice teachers higher perceived necessity of science education than general elementary preservice teachers. Second, there weren't also significant difference of science teaching attitude between two groups according to experiences of taking science related course. However elementary preservice teachers who are taking science course feel so easy toward comfort to science teaching compared to the not taking science course.

정신지체아를 위한 한국무용 적용가능성에 관한 연구

황명자,함정은 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.5

The aim of this study is to know the applicability of Korean Dance Program for children with mental retardation. It was used to refer various research and examination in order to accomplish the purpose of this study. The results were summarized as follows; First, the Korean Dance Program is a possibility program for children with mentalretardation when trying to consider the quality and an effect of the Korean Dance Program. Second, The SO-GO Dance and Mask Dance of Korean Dance Program was the best popular program by boys, SO-GO and Gang-Gang Sool Lae was the best popular program by girls.

주부들의 의복 관여도와 유아복 잡지광고에 대한 태도 고찰

황춘섭,오은정 慶熙大學校 1996 論文集 Vol.25 No.-

This research is interested in the concept of consumer's fashion involvement which makes influences on the consumer's information-searching pattern. The main object of the approach is the mothers of the babies under the age 3, who can be considered the actual babies' costumes buyers. The writer tried to find out whether the mother's own fashion involvement is in any proportionate relation with their babies' costumes involvement and also to study their various attitudes toward the magazine advertising of babies' costumes. The writer hope this research could provide babies' costumes industries with basic data that can help to establish more effectual advertising strategies. To achieve the purpose of the present research. The writer established 4 more detailed study points as follows: 1) to find out the appeal patterns of babies' costumes advertisements especially favored by the mothers 2) to find out the relationship between the mothers' own fashion involvement and their involvement in the babies'costumes advertisement 3) to find out their various degree of involvement in the babies' costumes advertisement according to their fashion involvement degree. This research was conducted through the survey method using stimulating objects and questionnaires. The experimental advertisements used in this survey were selected among the actual magazine advertising, and reproduced with the help of an expert in advertisement production who is a present member of an advertising company. During the reproduction process, all the brand names were deleted in order to control the brand familiarity and the brand preference which might have an influence on the consumer's attitude toward specific advertisements. The appeal patterns of the magazine advertising materials used in this research was limited to only 3 kinds that is, the appeal to reason, the mixed appeal to reason and emotion, the appeal to emotion. The questionnaire includes question items designed to identify the attitudes toward advertisements, the degree of fashion involvement, the opinions on utility of magazine advertising of babies costumes, and the present magazine subscription situation, the source of information on babies' costumes purchase, 394 samples were extracted through the convenience sampling method among the mothers of babies under the age 3. presently living in Seoul. The data was analyzed by Frequency, paired t-test, Chi-square, Correlation and Oneway. The result of the research can be summarized as follows: 1) Among the 3 kinds of advertisement appeal patterns, most preference was shown to the mixed appeal to reason and emotion. The appeal to emotion followed next and then comes the appeal to reason. 2) The higher the degree of mothers' own fashion involvement, the higher their involvement in their babies'costumes. 3) Mothers' fashion involvement degree doesn't make any difference in their attitudes toward the advertisement of babies' costumes.

肢발생중 세포 증식성에 미치는 Etretinate의 영향에 관한 연구

황정연,김은영,박선화,전용혁 고려대학교 의과대학 1994 고려대 의대 잡지 Vol.31 No.1

Cell proliferation in the developmental sequence of fore-limb in the DDY mouse with the adverse effect of single dose of etretinate (Tigason®) on it correlated to the drug-induced dysmorphogenesis was studied by measns of argyrophil technique for nucleolar organizer regions(AgNORs) and expression of proliferating cell nuclear antigen(PCNA). The number of AgNORs per nucleus displayed regional differences in the limb bud on day 11 of gestation, as higher values in apical ectodermal ridge(AER) and growing tip but decreased rapidly thereafter compared with those in the other parts of epithelia and subepithelial mesenchyme, while in core mesenchyme where the higest in its value on day 11, the numbers decreased depend not on the age but on the chondrified stage specific. Antibody reaction to PCNA expressed in almost all of the cells, stronger in mesenchyme than in epithelia on day 11 and then decreased gradually with the development proceed. Siver reactions to NORs as well as PCNA expression were decreased sharply by etretinate but recovered gradually from 24 hours after treatment except the value of AgNORs in the chondrified mesenchyme. The results Indicate that growth conducted by AER with subridge mesenchyme and chondrification in the core mesenchyme are two of major processes in the early development of limb, and the adverse effects of etretinate on proliferation and differentiation persist longer in the chondrified area but decreased expression of proliferative activities by the drug do not appear to be the result subsequent to the drug-induced cell death. The results suggest that temporal inhibition or interruption of the proliferation could deprive the programmed sequence of development of its appropriate timing with subsequent disturbance of the pace in growth which might be a role of limb dysmorphogensis but the drug's affinity to the chondrified cells remains a question.

뇌동맥류 파열에 의한 급성 경막하 혈종이나 뇌내혈종을 동반한 혼수상태 환자에 대한 뇌혈관 조영술을 시행하지 않은 응급수술

황수현,박용규,백선하,박인성,김은상,정진명,한종우 대한신경외과학회 1996 Journal of Korean neurosurgical society Vol.25 No.10

Neurologically based clinical grading scales offer excellent prognostic information for the patient suuffering diffuse subarachnoid hemorrhaging. These grading scales are less applicable to patients with life-threatening intraparenchymal or subdural hematomas after aneurysmal rupture. During 2 and half years. 9 patients(ICH;5.SDH:1. ICH+SDH:3) in a comatose state with brain stem compression syndromes documented by computerized tomographic scans have undergone emergent operation without angiography in our neurosurgical departement. Each patient was so critically ill that we believed it unwise to delay craniotomy for diagnostic angiography. The average delay from the detection to operation was 2 hours. The origin of the hematoma was identified as a berry aneurysm of the middle cerebral artery bifurcation in seven patients and a posterior communicating artery aneurysm in two patients. One patient had a good recovery. two patients recovered with hemipardsis and mild cognitive dysfunction. two patients were vegetative and died due to pneumonia. and four patients died due to brain swelling within two weeks postoperatively. Emergent craniotomy with empiric exploration of appropriate subarachnoid cisterns after hematoma decompression may be life-saving management in some cases. The delay imposed for diagnostic angiography may be avoided in attempts to save vital minutes of severe brain stem compression.

고립성 폐 결절로 나타난 경화성 혈관종의 폐엽 절제술에 의한 치험 1예 : A case report

황은구,이송암,황재준,김상윤,권혁중,김요한 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.13 No.-

Patient was sixty-eight years old female. She was transferred to our department to resect the solitary pulmonary nodule on right lower lobe. Intraoperative frozen-section revealed bronchioloalveolar carcinoma. So, we performed right lower lobectomy and mediastinal lymph node dissection. Permanent pathologic diagnosis of this patient was sclerosing hemangioma. We report experience of treatment of sclerosing hemagioma presenting solitary pulmonary nodule by lobectomy with the review of literatures.