척추 전이성 종양의 수술적 치료 : 삶의 질에 대한 평가를 중심으로 focused in evaluation of life quality

양준영,이준규,홍의표,안성환,이정범 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

목적 : 척추에 발생한 전이성 종양 환자들을 대상으로, 수술적 치료를 시행한 환자에 있어서, 술후 생존 기간과 생존 기간 내 동통, 신경학적 증상, 일상 생활 능력 등의 평가를 통한 수술적 치료의 결과에 대하여 보고하고자 한다 . 연구대상 및 방법 : 1996년부터 1999년까지 본원에서 전이성 종양으로 수술적 치료를 받았던 환자중 추시가 가능했던 13례의 환자를 대상으로 하였으며 술 후 생존 기간 및 술 전 및 술 후 추시 중의 동통 양상 및 신경학적 증상의 호전여부, 일상 생활 수행 능력을 분석하였다 . 결과 : 전례에서 술 전 동통을 호소하였으며, 1례에서만 단독 보행이 가능하였고, 1례에서는 앉는 것만 가능하였으며, 11례에서는 앉지도 못하였다. 술 후 평가에서는 전례에서 동통이 감소하였으며, 술전에 신경학적 증상이 보였던 환자 중 75%에서 신경학적 증상이 호전이 나타났으며, 대상환자 중 92%에서 자가 보행이 가능하거나, 앉는 것이 가능하게 되었다. 사망한 환자의 술후 생존기간은 평균 11.2 개월이었다. 결론 : 수술적 치료를 받은 척추의 전이성 골종양 환자에서 술 후 신경학적 증상의 호전, 동통의 감소 및 보행 능력의 호전을 볼 수가 있었다. 따라서 척추의 전이성 종양 환자에 있어, 수술의 적응증이 된다면 수술적 치료를 적극적으로 시행하는 것이 환자의 삶의 질을 향상시킬 수 있다고 사료된다. To evaluate the clinical results of surgical treatment in metastatic spinal tumor patients. Thirteen patients of spinal metastatic tumor, treated by surgical methods, in this hospital from 1996 to 1999, were included in this study. We analysed pre and post operative pain, neurologic symptom and life quality. Preoperatively, all patients complained of pain and one case do could self walking, one case could do self sitting, and eleven patients were bed-ridden states. Postoperatively, pain was subsided in all cases. Neurologic symptom recovered in 75% of patients who had had preoperative neurologic symptoms. Postoperatively, patients walk or sit without assistance in 92% of cases. Mean postoperative span of survival was 11.2 months. Surgical treatment of spinal metastatic tumor improved the neurologic status and reduced pain. So, we conclude that if indicated, surgical treatment seems to achieve better results than conservative treatment in spinal metastatic tumor patient.

A Comparative Study of Behavioral and immunohistological Changes after Spinal Cord Injury between Young and Adult Rats

Jun-Young Yang(양준영),June-Kyu Lee(이준규),Kwang-Jin Rhee(이광진),Kyung-Cheon Kim(김경천),Ui-Pyo Hong(홍의표),Jung-Bum Lee(이정범) 대한정형외과학회 2004 대한정형외과학회지 Vol.39 No.5

목적: 어린 쥐와 성숙 쥐의 척수 손상에서 나타나는 행동상과 면역 조직 화학상의 변화의 차이를 비교함으로써 척수 손상에서 나타나는 변화의 기전 차이를 설명하고자 하였다. 대상 및 방법: 생후 5주된 수컷 쥐 25마리와 16주된 수컷 쥐(Spraque-Dawley rat) 25마리가 사용되었다. 쥐들을 페노바비탈로 마취시킨 후 제 11번, 12번 흉추 위치에서 추궁절제술을 시행하였으며 개량된 뉴욕 대학 충격기(modified New York University impactor)를 이용하여 20 ㎜ 높이에서 10 gm의 충격을 줘서 척수 손상을 유발하였다. 이에 따른 배뇨장애를 방지하기 위해 매일 2회씩 손으로 눌러 방광을 비워주었으며, 수술을 받지 않은 쥐를 정상 대조군으로 사용하였다. 척수손상을 입은 쥐들에 대한 행동 시험을 Basso-Beatti-Bresnahan (BBB) 가산표를 이용하여 시행하였고, 행동 시험은 척수손상 후 제 1, 7일에 시행하였다. 쥐들의 손상된 척수를 수술 후 제 1, 7일에 절개하였으며, 척수 절편에 H-E 염색과 c-Jun과 GFAP유전자에 대한 면역조직화학 검사를 시행하였다. 면역 반응은 상온에서 1시간 동안 말초 혈액 도말 내의 avidinbiotin-peroxidase 복합체 내에서 그리고 0.05% 3,3 -diaminobenzidine과 0.01% 과산화 수소에서 5-10분간 반응 시켰다. 결과: 생후 5주된 쥐들의 뒷다리는 수술 1일 이후 마비되었고 수술 7일후에 부분적으로 회복되었으나, 생후 16주된 쥐들의 경우 수술 후 7일째에도 마비된 상태로 남아있었다. 생후 16주된 쥐들에서는 손상 받은 척수의 회백질에서 c-Jun유전자의 발현이 7일째까지 증가했으며, 생후 5주의 쥐들에서는 c-Jun유전자의 발현이 수술 1일째 상당히 증가하였으나, 회백질의 c-Jun유전자의 발현은 수상 후 7일째부터 감소하였다. 회백질의 GFAP유전자의 발현은 생후 16주의 성인 쥐들에서는 수상 후 제 1일째부터 증가되기 시작하였으나 수상 제 7일째부터 괴사부분에서 감소되기 시작하였고, 생후 5주의 어린 쥐들이 회백질에서는 GFAP유전자 발현이 수상 후 7일째까지 점진적으로 증가하였다. 결론: 나이가 많은 쥐들에서 척수 손상이 회복되지 않은 것은 척수 손상 후 c-Jun유전자 발현의 지속적인 상향조절 또는 GFAP유전자의 하향조절과 연관되어 있을 가능성을 제시한다. 반대로, GFAP유전자의 상향조절 또는 c-Jun유전자의 하향 조절은 동일한 손상을 받은 어린 쥐들에서 척수 손상의 부분 회복과 관련되어 있는 것으로 사료된다. Purpose: The aim of this study was to compare the behavioral and immunohistochemical changes in a spinal cord injury (SCI) between young and adult rats and to clarify the differences in the mechanism underlying the changes in a SCI between young and adult rats. Materials and Methods: A total of 25 young and 25 adult male Sprague-Dawley rats (5 weeks and 16 weeks old) were used. The rats were anesthetized with pentobarbital and laminectomies were carried out at the level of the 11th and 12th thoracic vertebra. Using a modified New York University Impactor, a SCI was induced by dropping a 10 gm weight at a height of 20 mm. The bladders were emptied manually twice a day to prevent urinary problems. The animals that received no surgery were used as the normal controls. Behavior tests were performed using the Basso-Beatti-Bresnahan (BBB) scoring system, 1 and 7 days after the weight drop injury. The difference in the BBB score between the young and adult rats were analyzed by a paired t-test with a p value <0.05 considered significant. The injured spinal cords were dissected at 1 and 7 days after surgery. H-E stain and immunohistochemistry for c-Jun and GFAP were performed in the spinal cord sections. The immunoreactions were visualized by incubation for 1 hour at RT in an avidin-biotin-peroxidase complex in PBS and 5-10 min in 0.05% 3,3 -diaminobenzidine and 0.01% H₂O₂ in 0.1 M PBS. Results: The hind limbs of young rats were paralyzed 1 day after surgery, but had recovered partially 7 days after surgery. However, the adult rats remained in the paralyzed status 7 days after surgery. The c-Jun expression level increased in the gray matter up to 7 days after the weight-drop injury in adult rats. The c-Jun expression level increased significantly in the gray matter 1 day after the injury in the young rats. However, the c-Jun expression level decreased significantly in the gray matter 7 days after the injury in the young rats compared with that of the 1 day post-injury. The GFAP expression level in the gray matter increased 1 day after the weigh-drop injury in the adult rats. However, it decreased in the necrotic region 7 days after the injury in the adult rats. GFAP expression in the gray matter increased gradually up to 7 days after the injury in the young rats. Conclusion: These results suggest that lack of recovery from a SCI in adult rats may be related to the continuous upregulation of c-Jun expression and/or the downregulation of GFAP after the weight-drop injury. In contrast, the upregulation of GFAP expression and/or the downregulation of c-Jun expression in the spinal cord might be related to a partial recovery in young rats after a weight-drop injury.

Preparation of Resveratrol-loaded Poly(ε-caprolactone) Nanoparticles by Oil-in-water Emulsion Solvent Evaporation Method

Bum-Keun Kim,Jun-Soo Lee,Ju-Kyoung Oh,Dong-June Park 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.1

Resveratrol-loaded poly(ε-caprolactone) (PCL) nanoparticles were prepared by oil in water (O/W) emulsion solvent evaporation method. The morphology of the nanoparticles was evaluated using atomic force microscope (AFM), in which well-shaped and rigid nanoparticles were prepared. The mean particle size of nanoparticles prepared using only dichloromethane (DCM) (523.5±36.7 ㎚) was larger than that prepared with a mixture of DCM and either ethanol (EtOH) (494.5±29.2 ㎚) or acetone (493.5±6.9 ㎚). The encapsulation efficiency of nanoparticles prepared only with DCM as dispersed phase (78.3±7.7%) was the highest of those prepared with solvent mixtures. An increase in the molecular weight of PCL led to an increase in encapsulation efficiency (from 78.3±7.7 to 91.4±3.2%). Pluronic F-127 produced the smallest mean size (523.5±36.7 ㎚) with the narrowest particle size distribution. These results show that dispersed phase, molecular weight of wall materials, emulsion stabilizer could be important factors to affect the properties of nanoparticles.

Changes in Pasting and Fluid Properties of Corn and Rice Starches after Physical Modification by Planetary Mill

Bum-Keun Kim,Jun-Soo Lee,Yong-Jin Cho,Dong-June Park 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.4

Corn and rice starches were physically modified by planetary mill. While native starches showed high peak viscosities (1,001 and 563 cp), it decreased largely (42 and 20 cp for rice and corn starch, respectively) after 2 hr of physical modification. When two starches were co-ground, peak viscosities decreased more largely than single ground one only in 30 min, indicating the pasting properties could be easily changed by co-grinding. Especially, the higher the amount of corn starch, the viscosity decreased more largely, which means that paste stability could be controlled also by changing the ratio of corn and rice starch. Mean particle size increased with physical modification time since particles became spread because of shear force. There were also changes in surface morphology after physical modification. Fluid property, such as mean time to avalanche (MTA), was improved (from 6.16±0.47 and 8.37±1.23 sec to 5.47±0.78 and 5.26±1.37 sec for rice and corn starch, respectively) by physical modification. Pasting property, such as swelling power, was also improved by physical modification. These mean that native starches can be applied to both conventional powder and new paste-food industry more efficiently by physical modification.

[P3-15] Influence of Soybean Hull-Coated Wheat Flour Composites on Low-Fat Uptake of Doughnuts

Jun-Soo Lee,Bum-Keun Kim,Kyung-Ae Lee,Seung-Ho Kim,Dong-June Park 한국식품영양과학회 2006 한국식품영양과학회 학술대회발표집 Vol.2006 No.10

마우스 사육에 있어서 깔집의 종류에 따른 기호도 및 재료성질 분석에 관한 연구

Jun-Sung Lim,Hyun-Suk Kim,Jae-Bum Ahn,Dae-Hun Park,Jong-Sung Park,Heui-Jin Kim,Jong-Sung Kim,Jin-Soo Han,Mi-Sook Lee,Ja-June Jang,Myng-Sang Kwon,Min-Jae Lee(이민재) 한국실험동물학회 2004 한국실험동물학회 학술발표대회 논문집 Vol.2004 No.-

슬관절 전 구획에 발생한 수지상 지방종의 관절경적 치료

이준규(June-Kyu Lee),김영모(Young-Mo Kim),이광진(Kwang-Jin Rhee),황득수(Deuk-Soo Hwang),양준영(Jun-Young Yang),주용범(Yong-Bum Joo),양재훈(Jae-Hoon Yang),권순태(Soon-Tae Kwon) 대한정형외과학회 2005 대한정형외과학회지 Vol.40 No.4

수지상 지방종은 활액막에서 지방 조직이 융모상으로 증식하는 질환으로, 발생 원인으로 외상, 반월판 손상, 염증, 감염, 퇴행성 변화, 종양 등이 알려져 있다. 이 질환에 대한 보고는 매우 드물다. 저자들은 관절경적 수술로 양호한 치료 결과를 보인 슬관절 전 구획에서 발생한 수지상 지방종 1예를 보고한다. Lipoma arborescens is a villous proliferation of fatty tissue in the synovial membrane, and known causes are trauma, meniscal injury, inflammation, infection, degenerative change, tumor, and so forth. The reported cases are extremely rare. We report a case of lipoma arborescens developed in all compartments of the knee joint, and treated well with arthroscopic surgery.

History of Acquired Immune Deficiency Syndrome in Korea

Kim June Myung,Kim Nam Joong,Choi Jun Yong,Chin Bum Sik 대한감염학회 2020 Infection and Chemotherapy Vol.52 No.2

The first human immunodeficiency virus (HIV) infection was reported in Korea in 1985. The number of HIV-infected persons domestically increased in the 1990s showing epidemic indigenousization. Since then, the number of new infections gradually increased every year, and recently more than 1,000 newly infected cases were reported per year. A total of 12,522 infected individuals have been reported up to 2015, of which 2,020 died. The male to female ratio was 15.4:1, and 34.2% of them were under 30 years old. The infection route was homosexual and bisexual contact in 60.1% of cases and heterosexual contact in 34.6% of cases. Candidiasis, Pneumocystis pneumonia, tuberculosis were common as a AIDS (acquired immune deficiency syndrome)-defining illness. But with the introduction of antiretroviral therapy in the late 1990s, non-AIDS defining illnesses such as metabolic complications, cardiovascular diseases, bone diseases, and neuropsychiatric disorders such as neurocognitive dysfunction, depression, and anxiety are emerging as new health problems. The management policy switched its focus from regulating and monitoring of HIV-infected persons to ensuring access to treatment and promotion of voluntary HIV testing in high-risk groups. Also as the age of the infected persons increases, a need for various supports such as social rehabilitation, life counseling, and welfare has emerged.

Utility of QuantiFERON-TB In-Tube Test for Differentiating Active Tuberculosis from Latent Tuberculosis Infection in an Intermediate Burden Country

최석훈,Bum Sik Chin,Sang Hoon Han,Han Sung Lee,Chang Oh Kim,Su Jin Jeong,Hee Kyung Choi,Myung Soo Kim,Jun Yong Choi,Young Goo Song,June Myung Kim 대한감염학회 2008 Infection and Chemotherapy Vol.40 No.6

Background : The aim of the present study was to assess the contribution of a QuantiFERON-TB Gold In-Tube test (QFT-IT) in differentiating active tuberculosis (TB) from latent tuberculosis infection (LTBI) by quantifying interferon-γ levels. Materials and Methods : We retrospectively reviewed clinical records of 314 patients older than 15 years who had performed QFT-IT between July 2006 and August 2007 at a tertiary care teaching hospital. Results : Subjects with active TB (n=81, culture confirmed active TB in 40 subjects) and LTBI (n=76) were included. Mean±SD IFN-γ levels were 4.96±3.98 IU/mL (range -0.08-10) for all subjects with active TB, 4.54±4.05 IU/mL (range -0.08-10) for culture confirmed active TB, and 4.11±3.57 IU/mL (range 0.35-10) for subjects with LTBI. The quantitative results of QFT-IT on IFN-γ levels between all the subjects with active TB and those with LTBI were not statistically significant (P=0.16). The result was similar when compared between those with culture confirmed active TB and those with LTBI, showing little statistical significance (P=0.554). Conclusion : The production of IFN-γ measured by QFT-IT showed no correlation between its level and the activity of Mycobacterium tuberculosis infection. These results suggest that measuring IFN-γ using QFT-IT might not be useful for distinguishing active TB from LTBI. Background : The aim of the present study was to assess the contribution of a QuantiFERON-TB Gold In-Tube test (QFT-IT) in differentiating active tuberculosis (TB) from latent tuberculosis infection (LTBI) by quantifying interferon-γ levels. Materials and Methods : We retrospectively reviewed clinical records of 314 patients older than 15 years who had performed QFT-IT between July 2006 and August 2007 at a tertiary care teaching hospital. Results : Subjects with active TB (n=81, culture confirmed active TB in 40 subjects) and LTBI (n=76) were included. Mean±SD IFN-γ levels were 4.96±3.98 IU/mL (range -0.08-10) for all subjects with active TB, 4.54±4.05 IU/mL (range -0.08-10) for culture confirmed active TB, and 4.11±3.57 IU/mL (range 0.35-10) for subjects with LTBI. The quantitative results of QFT-IT on IFN-γ levels between all the subjects with active TB and those with LTBI were not statistically significant (P=0.16). The result was similar when compared between those with culture confirmed active TB and those with LTBI, showing little statistical significance (P=0.554). Conclusion : The production of IFN-γ measured by QFT-IT showed no correlation between its level and the activity of Mycobacterium tuberculosis infection. These results suggest that measuring IFN-γ using QFT-IT might not be useful for distinguishing active TB from LTBI.

임플란트 저작능에 영향을 주는 신경학적 원인에 대한 고찰

윤준호(Jun-ho Yoon),김태선(Tae-Seon Kim),김성회(Sung-Hoi Kim),김지환(Jee-Hwan Kim),이재훈(Jae-Hoon Lee),심준성(June-sung Shim),문홍석(Hong-Suk Moon),박영범(Young-Bum Park) 대한턱관절교합학회 2012 구강회복응용과학지 Vol.28 No.3