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임윤규,이두식,박전홍,양기천,김승호,김공식,현관종,김우택,이영순 濟州大學校 農科大學 動物科學硏究所 1993 動物科學論叢 Vol.8 No.1
Enzyme-linked Immuno sorbent Assay (ELISA) for the serological diagnosis oi Bruceiia abortus was developed and compared with plate aggluhnation test Cell wall antigen was extracted from Brucella abortus 1119-3 by sonicabon and with a sodium deoxychlate solution Optimum protein concentra tion of coating antigen 0.4㎍/100㎕ protein on each microtiter plate well. Horse radish peroxidase(HRP) labled protein-G was used as a tracer of reacted antibodies ELISA confirmed the agreeable results of 40 rases out of 43 cases by plate aggulutination test ELISA diagnosed positive cases (10 out of 12) and negatiw cases (1 out of 12) with dubious sera by plate agglutination test From this results EL ISA could be used for the early diagnostic tools of Brucellosis in cattle.
흰쥐 적출 자궁의 수축 작용과 흰쥐 장관에 있어 칼륨에 의해 활성화되는 칼슘 채널에 대한 Cyclobuxine D의 영향
권준택(Jun-Tack Kwon),이종화(Jong-Hwoa Lee),박영현(Young-Hyun Park),조병현(Byung-Heon Cho),최규홍(Kyu-Hong Choi),김유재(Yu-Jae Kim),김종배(Jong-Bae Kim),김정목(Chung-Mok Kim),김천숙(Chun-Sook Kim),차영덕(Young-Deog Cha),김영석(Young- 대한약리학회 1988 대한약리학잡지 Vol.24 No.1
Buxus microphylla var. koreana Nakai에 존재 하는 steroidal alkaloid인 cyclobuxine D는 흰쥐에 있어 심박동수 감소 작용, 적출 개구리 심장에서 수축력 감소작용, 토끼 적출 장관에서 acetylcholine과 Ba<sup>++</sup>.에 유발되는 수축에 대한 억제작용 등을 나타낸다고 보고되었다. 본 연구에서는 흰쥐 적출 자궁에서 acetylcholine, oxytocin과 Ba<sup>++</sup>에서 의해 나타나는 수축 작용에 대한 cyclobuxine D의 영향을 관찰하였으며, 또 흰쥐 적출장관에서 칼륨에 의해 활성화되는 칼슘채널에 대한 cyclobuxine D의 작용을 관찰하였다. Cyclobuxine D는 흰쥐 적출 자궁에서 acetylcholine, oxytocin과 Ba<sup>++</sup>에 의해 증가되는 peak tension과 duration을 용량적으로 현저히 억제하였다. Cyclobuxine D는 oxytocin보다 acetylcholine에 의해 나타나는 수축작용에 대해 강하게 작용했다. 흰쥐 적출 장관(ileum)을 Ca을 고갈시킨 Tyrode s 용액에 40 ~ 50분 담그고 Na<sup>+</sup> 대신 K<sup>+</sup>로 대체시킨 용액에 10분간 담근 후 1.8 mM CaCl<sub>2</sub>를 가했을 때 이중적인 근육수축작용이 나타난다(Phasic and tonic increase in tension). Cyclobuxine D (6.2 × 10<sup>-5</sup> M)은 이 두 components를 유의하게 억제하였으며 tonic component가 최대치에 도달했을 때 cyclobuxine D (3.1 X 10<sup>-4</sup> M)을 가하면 근육은 긴장도를 빨리 상실했다. 이 결과는 적출 장관에 있어 칼륨에 의해 활성화되는 칼슘 채널이 cyclobuxine D에 의해 차단되고 있음을 나타낸다. 이상의 결과에서 cyclobuxine D의 흰쥐 적출 자궁에 대한 수축 억제 작용은 voltage-dependent calcium channel 차단에 밀접한 관련이 있는 것으로 사려된다. Cyclobuxine D, extracted from Buxus microphylla var. koreana Nakai, is a steroidal alkaloid. Many pharmacological effects of cyclobuxine D were examined in our Lab. Cyclobuxine D showed a significant bradycardic effect in the rat heart and an inhibitory action on acetylcholine and Ba<sup>++</sup>-induced contraction of the longitudinal muscle isolated from the rabbit jejunum. In this study, we investigated the effect of cyclobuxine D on the contractile response-elicited by acetylcholine, oxytocin and Ba<sup>++</sup> in rat uterine. In order to analyse the inhibitory action of cyclobuxine D on the smooth muscle, we examined the inhibitory action of cyclobuxine D against the contractile response of the high potassium-depolarized rat ileum to calcium. Concentration-dependent decrease in the peak tension and duration of the acetylcholine, oxytocin and Ba<sup>++</sup>-induced contraction in the isolated rat uterus was observed when cyclobuxine D was added to the organ bath. The isolated longitudinal muscle from the rat ileum was immersed calcium-depleted potassium-depolarizing solution. Ten minutes after, 1.8 mM CaCl<sub>2</sub> was added to muscle bath and elicited a biphasic increase in muscle tension. Cyclobuxine D (6.2 × 10<sup>-5</sup> M) produced an appreciable inhibition of both components of the mechanical response. In addition, 3.1 × 10<sup>-4</sup> M cyclobuxine D, introduced at a point when the tonic response had reached its maximum level, caused the muscle to exhibit a rapid lose of tension. Based on these experimental results, we propose the possibility that the inhibitory action of cyclobuxine D on the acetylcholine, oxytocin and Ba<sup>++</sup>-induced contraction in the isolated rat uterus may be due to blocking potassium-activated calcium channels, voltage-sensitive calcium channels.
Hua Hong,Jeong Rang Jo,Ji Hyeon Yeon,Jun Tack Hong,Kyung Hwan Jung,Sun Kyun Yoo,Byeong Churl Jang 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.2
The study objective was to prepare biodegradable branched dextran microspheres encapsulated with His-tagged interferon-alpha (BDM-hIFN-α) and evaluate its activity in vitro and in vivo. The glycidyl methacrylate derivatized dextrans (Dex-GMA) as precursor was primarily synthesized by substituting hydroxyl groups of either the branched or linear type of dextran with GMA. Dex-GMA microspheres loaded with hIFN-α was then prepared by the water-in-water emulsion technique. In vitro release and Western blotting experiments demonstrated the retained activity of hIFN-α released from branched dextran microspheres at 24 h by inducing phosphorylation of signal transducer and activator transcription-1 (STAT-1), a down-stream effector of IFN-α, in HepG2 cells. Animal data further revealed a peak of plasma levels of IFN-α in rats injected intravenously with BDM-hIFN-α at 10 min post-injection, but a sharp decline at 2 h. High plasma levels of neopterin, a plasma protein induced by IFN-α, were also detected in rats injected with BDM-hIFN-α at 10 min post-injection. Notably, plasma levels of neopterin remained high at 4 h, but largely declined thereafter.
Kim, Tack-Joong,Han, Hyeong-Jun,Hong, Seong-Su,Hwang, Ji-Hye,Hwang, Bang-Yeon,Yoo, Hwan-Soo,Jin, Yong-Ri,Lee, Jung-Jin,Yu, Ji-Yeon,Lee, Kwang-Ho,Kang, Byoung-Won,Yun, Yeo-Pyo Pharmaceutical Society of Japan 2007 Biological & pharmaceutical bulletin Vol.30 No.4
<P>Platelet derived growth factor (PDGF)-BB is one of the most potent vascular smooth muscle cell (VSMC) proliferative factors, and abnormal VSMC proliferation by PDGF-BB plays an important role in the development and progression of cardiovascular problems, including restenosis after coronary angioplasty and atherosclerosis. Previous phytochemical studies on the stems or root barks of <I>Cudrania tricuspidata</I> (Moraceae) resulted in the isolation of various isoprenylated xanthones and flavonoids, some of which have anti-cancer, hepatoprotective, anti-inflammatory and anti-oxidant activities. In the present study, we investigated the antiproliferative effect of cudratricusxanthone A isolated from the root bark of <I>C. tricuspidata</I> and its underlying mechanism in VSMCs. Antiproliferative effects of cudratricusxanthone A on VSMCs were examined by direct cell counting and [<SUP>3</SUP>H]-thymidine incorporation assays. Cudratricusxanthone A inhibited [<SUP>3</SUP>H]-thymidine incorporations into DNA in VSMCs that occurred in response to treatment with 50 ng/ml PDGF-BB. PDGF-BB-stimulated DNA synthesis was significantly reduced to 86.1, 80.2, 64.2 and 25.1% at concentrations of 0.1, 1, 2 and 3 μ<SMALL>M</SMALL>, respectively. Moreover, pre-treatment with cudratricusxanthone A (0.1—3 μ<SMALL>M</SMALL>) suppressed this PDGF-BB-stimulated cell number in a concentration-dependent manner. The inhibition percentages were 11.1, 22.7, 51.3 and 81.5% at the concentrations of 0.1, 1, 2 and 3 μ<SMALL>M</SMALL>, respectively. We also investigated the mechanism of antiproliferative effects by cudratricusxanthone A in PDGF-BB-stimulated VSMCs. In Western blot analysis, 50 ng/ml PDGF-BB-stimulated phospholipase C (PLC)γ1, Ras, and extracellular signal-regulated kinase1/2 (ERK1/2) phosphorylations were inhibited by cudratricusxanthone A (0.1—3 μ<SMALL>M</SMALL>). Consisted with these findings, cudratricusxanthone A inhibited PDGF-receptor β chain (PDGF-Rβ) phosphorylation induced by PDGF-BB in a concentration-dependent manner. These findings suggest that the inhibitory effects of cudratricusxanthone A on DNA synthesis and proliferation by PDGF-BB-stimulated VSMCs are mediated by the suppressions of the PDGF-Rβ and its downstream signaling pathways. Our observation may explain in part mechanistic basis for the prevention of cardiovascular diseases, such as atherosclerosis and restenosis after coronary angioplasty by cudratricusxanthone A.</P>
이억석,홍승연,전윤탁 경성대학교 1985 부산수산대학 논문집 Vol.6 No.2
The substitution reactions of methyl para-substituted benzenesulfonates with nucleophils such as methanol, pyridine and sodium methoxide in methanol at 30℃ were investigated by conductometry and spectrometry in order to understand the reactivityselectivity relationship. The Hammett plot of kinetic data showed ρ values of 1.29, 1.18, 1.04 for the nucleophilic substitution reaction with methanol, pyridine and methoxide, respectively. Taking ρvalues as a selectivity parameter of the nucleophiles and considering the results with a series of nucleophiles showing the increase of reactivity in that order, there seems to be an inverse relationship between reactivity and selectivity.
Son, Dong Ju,Kang, Jun,Kim, Tack Joong,Song, Ho-Sueb,Sung, Ki-Joong,Yun, Do Young,Hong, Jin Tae TAYLOR & FRANCIS 2007 JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH PAR Vol.70 No.15-16
<P> Studies previously reported that melittin, a major bioactive component of bee venom, inhibits vascular smooth muscle cell (VSMC) proliferation through suppression of nuclear factor (NF)-&kgr;B and Akt activation and through enhancement of proapoptotic protein expression. In this study, the effects of melittin were investigated on the tyrosine phosphorylation of platelet-derived growth factor (PDGF) beta receptor (R&bgr;) and its downstream intracellular signal transduction. When combined with PDGF-R&bgr; inhibitor, melittin exhibited a synergic inhibitory effect on PDGF-BB-induced rat aortic VSMC proliferation. In addition, melittin inhibited PDGF-R&bgr; phosphorylation in a concentration-dependent manner. Accordingly, the downstream signal transduction of PDGF-R&bgr;, such as ERK1/2, Akt, and PLC&ggr;1 phosphorylation, was also inhibited by melittin in the same manner. These findings suggest that, in addition to suppressing NF-&kgr;B activation, the antiproliferative effect of melittin in VSMC may be mediated, at least in part, by the inhibition of PDGF-R&bgr; tyrosine phosphorylation and its downstream intracellular signal transduction.</P>
Kim, Hyung-Sik,Yeon, Hong-Won,Choi, Mi-Hyun,Kim, Ji-Hye,Choi, Jin-Seung,Park, Jang-Yeon,Jun, Jae-Hoon,Yi, Jeong-Han,Tack, Gye-Rae,Chung, Soon-Cheol Gordon and Breach Science Publishers 2013 Computer methods in biomechanics and biomedical en Vol.16 No.5
<P>In this study, a tactile stimulator was developed, which can stimulate visual and auditory senses simultaneously by using the E-Prime software. This study tried to compensate for systematic stimulation control and other problems that occurred with previously developed tactile stimulators. The newly developed system consists of three units: a control unit, a drive unit and a vibrator. Since the developed system is a small, lightweight, simple structure with low electrical consumption, a maximum of 35 stimulation channels and various visual and auditory stimulation combinations without delay time, the previous systematic problem is corrected in this study. The system was designed to stimulate any part of the body including the fingers. Since the developed tactile stimulator used E-Prime software, which is widely used in the study of visual and auditory senses, the stimulator is expected to be highly practical due to a diverse combination of stimuli, such as tactile-visual, tactile-auditory, visual-auditory and tactile-visual-auditory stimulation.</P>