Pollen metabarcoding reveals the floral resources and niche overlap of honeybees in forest areas

Do Yuno,Park Woong‐Bae,Park Jun‐Kyu,Kim Chang‐Jun,Kim Il‐Kwon,Choi Moon Bo 한국곤충학회 2022 Entomological Research Vol.52 No.12

We investigated the floral resources of the honeybee (Apis mellifera)bypollenDNA metabarcoding as the first step in honeybee conservation. We attempted to identify as many floral resources and as wide resource availability as possible in areas and compared the richness of floral resources between forests and national parks. We found that both forest and national parks can sufficiently support honeybee floral resources; however, national parks were more stable habitats with more complex vegetation structure than those of forests. Also, honeybee’s preferences for floral resources were determined using niche breadth, niche overlap, and network analysis among the floral resources in forest areas. Quercus and Actinidia were the most frequently detected taxa of pollen resources in both forests and national parks by pollen metabarcoding. Even though we anticipated that more various pollen resource taxa would be found in national parks than forests, no differences in the number pollen resource taxa were detected between them. According to the niche overlap analysis result, the honeybee preferred the pollen resources in both forests and national parks, and the co-occurrence pattern analysis of pollen resource taxa in both forests and national parks showed a simple network. Altogether, our findings suggested that DNA metabarcoding of pollen collected by the honeybee can provide useful information on the flowering plants in a given area. As the use of floral pollen resources varies spatially and temporally, periodic monitoring of pollen resources can identify which pollen is required to maintain the health of honeybee colonies.

관동맥질환에서 저밀도 지단백 입자 크기의 분포

임효영,김인숙,승기배,강동헌,전두수,장기육,박준철,이수영,홍순조,최규보 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.8

한국 정신장애의 역학 조사 연구[I] : 각 정신장애의 유병률

조맹제,함봉진,김장규,박강규,정은기,서동우,김선욱,조성진,이준영,홍진표,최용성,박종익,이동우,이기철,배재남,신정호,정인원,박종한,배안,이충경 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.4

Objectives : This study aims to estimate the prevalence of the DSM-IV psychiatric disorders in Korean population using the Korean version of Composite International Diagnostic Interview (K-CIDI). Methods : Subjects were selected by taking multi-stage, cluster samples of 7,867 adult household residents, 18 to 64 years of age, in ten catchment areas. Total 78 trained interviewers administered the K-CIDI to the selected respondents, from June 1 to November30,2001. Results : Total 6,275 respondents completed the interview. Some 33.5% of respondents reported at least one lifetime disorder, 20.6% reported at least one-year disorder, and 16.7% reported at least one-month disorder. The most common lifetime disorders were alcohol abuse/dependence (17.24%), nicotine dependence/withdrawal (11.19%), specific phobia (5.16%), and major de-pressive disorder (4.25%). The lifetime prevalence of substance abuse/dependence (0.25%) and schizophrenia (0.16%) was very low. Nicotine and alcohol use disorder showed very high male/female ratio. Mood disorder and anxiety disorder were more prevalent among female than male. Conclusion : The prevalence of psychiatric disorders was high. In comparison with other studies, remarkable differences in distributions of psychiatric disorders across the areas and times were observed.

Protein kinase A-induced phosphorylation at the Thr154 affects stability of DJ-1

Ko, Yeon Uk,Kim, Su-Jeong,Lee, Juhyung,Song, Min-Young,Park, Kang-Sik,Park, Jun Bae,Cho, Hyun-Soo,Oh, Young J. Elsevier 2019 Parkinsonism & related disorders Vol.66 No.-

<P><B>Abstract</B></P> <P><B>Introduction</B></P> <P>Most cases of Parkinson's disease (PD) are sporadic, but genetic variations have been discovered in PD patients. PARK7/DJ-1 is a known cause of early-onset autosomal-recessive PD and is implicated in neuroprotection against oxidative stress. Although several post-translational modifications of DJ-1 have been proposed, phospho-modification of DJ-1 and its functional consequences have been less studied.</P> <P><B>Methods</B></P> <P>Putative phosphorylation sites of DJ-1 were determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS analysis). Subsequently, phosphorylation site of DJ-1 was confirmed by <I>in vitro</I> kinase assay and cell-based pull-down assay. Impaired dimer formation of phospho-null mutant was measured using DSS crosslinking assay and immunoprecipitation assay. To evaluate physiological consequences of this event, protein stability of DJ-1 WT and DJ-1 phospho-null mutant were compared using cycloheximide chase assay and ubiquitination assay.</P> <P><B>Results</B></P> <P>Here, we showed that DJ-1 directly bound to the catalytic subunit of protein kinase A (PKAcα). We found that PKAcα is responsible for phosphorylation of DJ-1 at the T154 residue. Interestingly, dimerization of DJ-1 was not detected in a DJ-1 T154A mutant. Furthermore, stability of the DJ-1 T154A mutant was dramatically reduced compared with that of wild-type DJ-1. We found that DJ-1 T154A was prone to degradation by the ubiquitin proteasome system (UPS).</P> <P><B>Conclusion</B></P> <P>We identified a novel phosphorylation site of DJ-1. Furthermore, we determined protein kinase A that is responsible for this posttranslational modification. Finally, we demonstrated physiological consequences of this event focusing on dimerization and protein stability of DJ-1.</P> <P><B>Highlights</B></P> <P> <UL> <LI> PKAcα phosphorylates PARK7/DJ1 at the Thr154 residue. </LI> <LI> Disruption of Thr154 phosphorylation is linked to a lower stability. </LI> </UL> </P>

전동공구 내구성 평가 실험장치 개발

박근석,변영철,강이석,강배희,이동욱,차원준 忠南大學校 産業技術硏究所 1997 산업기술연구논문집 Vol.12 No.2

For the endurance test, it is necessary to test the portable electric tools under the same condition as the actual operation condition. It is also essential that the test equipment is convenient to fix the tool. Those are necessary to control the loaded torque and to acquire and analyze the test data with a computer. In this study, we have developed an endurance test equipment for portable electric tools, where a hydraulic control system with a proportional relief valve was applied to control the load for the test. The developed test equipment used an industrial hydraulic pump as a load supplier to ensure a firm endurance of the test equipment itself. The data acquisition system has been automated by composing with a personal computer and RS-232C communication nets. This test equipment reduced the manhour for the test and also improved the reliability of the tested data which were measured and graphed.

알레르기성 비염 진단에서의 MAST CLA 결과

박윤근,류준선,윤창배,김기식,하경임,고병원 東國大學校 2000 東國論叢 Vol.39 No.-

Multiple allergosorbent test chemiluminescent assay (MAST CLA) is a simple method for in vitro measurement of allergen-specifie IgE antibodies. Total 125 patients was evaluated with Korean inhalant panel in diagnosis of allergic rhinitis. Overall positive rate was 53.6% and high incidence of allergen is house dust mite, house dust, cow's milk, mugwort, rye and brich. The results was slightly different from the results of large cities and the problem is absent item of domestic common trees. MAST CLA needs further improvement to be used as a primary screening test for allergy in Korea.

甘豆湯 전탕액과 구성약물이 배양심근세포 박동수에 미치는 영향

박준배,권강범,황인진,김우경,김희찬,오광수,이호섭,류도곤 대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.4

To test the protective effect of herbal medicine on myocardial damage against adriamycin-induced myocardiotoxicity, cytotoxicity of adriamycin was examined using MTT assay and protective effect of herbal medicine in the presence of water extract of Gamdu-tang, Gamigamdu-tang, or single constituents of these prescriptions was examined using counting of beating rate. The results of these experiments were obtained as follows : Adriamycin resulted in a decrease in viability and beating rate in cultured myocardial cells. Gamdu-tang water extract shows effects of protection from the cardiocyte toxicityinduced by adriamycin treatment such as increases beating rate. Gamigamdu-tang water extract shows the similar protective evvect as shown in gamdu-tang treatment on the decreasing of beating rate induced by adriamycin. Radix Glycyrrhizae, Semen Glycine, and Folium Phyllostachyos water extract shows a significant effect of protection for cardiotoxicity induced by adriamycin in cultured myocardial cells. These results show that adriamycin elicits toxic effects in cultured myocardial cells derived from neonatal mouse, and suggest teat water extract of gamdu-tang, gamigamdu-tang, Radix Glycyrrhizae, Semen Glycine, or Folium Phyllostachyos is very effective in the prevention of adriamycin-induced cardiotoxicity/

An Exact Triangle induced by a Short Exact Sequence which Splits

박상호,전영배 한국수학교육학회 1985 수학교육 Vol.24 No.1

닭에서 오존수의 급여가 닭와포자충 감염에 미치는 영향

박배근,染谷梓,류시윤,김현수,조성환,전무형,손화영 충남대학교 수의과대학 동물의과학연구소 2001 動物醫科學硏究誌 Vol.9 No.-

Ozone is very effective in acute and chronic viral disease as a virucidal agent and in improving the general health of the animals. The effects of ozonized water supply against Cryptosporidium baileyi was studied in 2-day-old chicken. After oral inoculation of chicken with 1×10^6 oocysts, ozonized water(0.5ppm) and tap water were supplied to experimental and control group, respectively. The prepatent period of both group was 6 days postinoculation(PI). The patent period of experimental group was shorter(6-18 days) than that of control group(6-22 days). The number of oocysts in the feces were maximum on days 11 PI, indicating 1.99×10^6 oocysts/experimental chicken and 6.00×10^6 oocysts/control chicken. The number of discharged oocysts during experimental period was higher in control group than that of experimental group. In the view of febric bursa sections on 7 days PI, the number of endogenous development of C baileyi was smaller in experimental group that in control group. This results indicate that the autoinfected sporozoite were damaged by ozone. The ozonized water supply to chickens is alleviation cryptosporidiosis.

충남 서북부지역의 개발 잠재력 분석 : 아산시를 중심으로

배성의,박동진,윤준상,황인극,최승철 공주대학교산업개발연구소 2000 産業開發硏究 Vol.8 No.-

This study was purpose for develop potential analysis of Chungnam north-west area and propose to regional development strategy. To arrived at a result for develop direction of Chungnam north-west area, Asan city will be development of central city for Asan bay, new indusrial city, tourist industrial city and ecological city.