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Heshouwu (Polygonum multiflorum Thunb.) Extract Attenuates Bone Loss in Diabetic Mice
Ju Ri Ham,Hae-In Lee,Ra-Yeong Choi,Hyo-Seon Ryu,Sung-Tae Yee,Kyung-Yun Kang,Mi-Kyung Lee 한국식품영양과학회 2019 Preventive Nutrition and Food Science Vol.24 No.2
This study investigated the effects and mechanism of Heshouwu (Polygonum multiflorum Thunb.) water extract (HSW) on diabetes-related bone loss in mice. HSW was orally administered (300 ㎎/㎏ body weight) to high-fat diet and streptozotocin-induced diabetic mice for 10 weeks. HSW significantly alleviated mouse body weight loss and hyperglycemia compared with the control group, and elevated serum levels of insulin, osteocalcin, and bone-alkaline phosphatase. HSW supplementation also significantly increased the bone volume/tissue volume ratio and trabecular thickness and number, and decreased the bone surface/bone volume ratio and trabecular structure model index in the femur and tibia. Moreover, HSW significantly increased femoral bone mineral density. In addition, HSW down-regulated osteoclastogenic genes, such as nuclear factor of activated T-cells, cytoplasmic 1 and tartrate-resistant acid phosphatase 5 (TRAP), in both the femur and tibia tissue, and reduced serum TRAP level compare to those of control mice. These results indicate that HSW might relieve diabetes-related bone disorders through regulating osteoclast-related genes, suggesting HSW may be used as a preventive agent for diabetes-induced bone loss.
Lee, Ju-Won,Park, Won-Seok,Gong, Hyo-Young,Lee, Ae-Ri,Kim, Da-Eun,Baek, Seung-Chon,Lee, Jong-Yeol Korean Society of Soil and Groundwater Environment 2013 지하수토양환경 Vol.18 No.6
The scope of this study was to develop, design, and build an ex-situ remediation system of using the heating and water sparging treatment for the highly volatile DNAPL (Dense Non-Aqueous Phase Liquid) contaminated groundwater, and to conduct pilot testing at the site contaminated with DNAPL. The TCE (Trichloroethylene) removal was at the highest rate of 94.6% with the water sparging at $70^{\circ}C$ in the lab-scale test. The pilot-scale remediation system was developed, designed, and fabricated based on the results of the lab-scale test conducted. During the pilot-scale testing, DNAPL-contaminated groundwater was detained at heat exchanger for the certain period of time for pre-heating through the heat exchanger using the thermal energy supplied from the heater. The heating system supplies thermal energy to the preheated DNAPL-contaminated groundwater directly and its highly volatile TCE, $CCl_4$ (Carbontetrachloride), Chloroform are vaporized, and its vaporized and treated water is return edback to the heat exchanger. In the pilot testing the optimum condition of the HWSRS was when the water temperature at the $40^{\circ}C$ and operated with water sparging concurrently, and its TCE removal rate was 90%. The efficiency of the optimized HWSRS has been confirmed through the long-term performance evaluation process.
Hydrogen Peroxide Induces Vascular Permeability via Regulation of Vascular Endothelial Growth Factor
Lee, Kyung Sun,Kim, So Ri,Park, Seoung Ju,Park, Hee Sun,Min, Kyung Hoon,Lee, Min Hee,Jin, Sun Mi,Jin, Gong Yong,Yoo, Wan Hee,Lee, Yong Chul American Thoracic Society 2006 AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR Vol.35 No.2
<P>Oxidative stress plays critical roles in initiation and/or worsening of respiratory disease process. Although reactive oxygen species (ROS) are shown to cause vascular leakage, the mechanisms by which ROS induce an increase in vascular permeability are not clearly understood. In this study, we have used a murine model to evaluate the effect of hydrogen peroxide (H(2)O(2)) to examine roles of ROS and the molecular mechanism in vascular permeability. The results have revealed that ROS levels, vascular endothelial growth factor (VEGF) expression, hypoxia-inducible factor-1alpha protein level, airway hyperresponsiveness, and vascular permeability are increased after inhalation of H(2)O(2). Administration of antioxidants markedly reduced plasma extravasation and VEGF levels in lungs treated with H(2)O(2). These results indicate that ROS may modulate vascular permeability via upregulation of VEGF expression.</P>
Ju Ri Ham,Yongjin Lee,Young-Jin Son,Ra-Yeong Choi,Mi-Kyung Lee 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
Edible insects are attracting attention as a sustainable food resource in future food. Recently, researches on the relationship between bone health and chronic diseases are increasing. Therefore, this study attempted to determine the anti-osteoporosis effect of Tenebrio Molitor larva fermentation extract (TM). The TRAP staining of bone marrow macrophage cells indicated that TM dose-dependently inhibited osteoclastogenesis compared with the vehicle. TM treatment down-regulated NFATc1, DC-STAMP, OSCAR, Ctsk, and Trap mRNA expression. NFATc1, a major protein in osteoporosis, level was decreased by the TM treatment. The ALP staining of C2C12 cells indicated that TM dose-dependently increased osteoblastogenesis. In high-fat fed mice, TM supplementation significantly increased femoral and tibial bone mineral density. These results demonstrate the anti-osteoporotic effects of TM by regulating osteocalstogenesis and osteoblastogenesis, which provided useful information for its application and development against obesity-induced osteoporosis.
Lee, Kyung Sun,Kim, So Ri,Park, Seoung Ju,Lee, Ho Kyung,Park, Hee Sun,Min, Kyung Hoon,Jin, Sun Mi,Lee, Yong Chul American Society for PharmacologyExperimental Ther 2006 Molecular pharmacology Vol.69 No.6
<P>Vascular endothelial growth factor (VEGF) plays a pivotal role in the pathogenesis of bronchial asthma. Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) has been implicated in regulating cell survival signaling through the phosphoinositide 3-kinase (PI3K)/Akt pathway. The key role of PI3K in VEGF-mediated signal transduction is established. However, the effects of PTEN on VEGF-mediated signaling in asthma are unknown. This study aimed to determine the effect of PI3K inhibitors and PTEN on VEGF expression in allergen-induced airway inflammation. We have used a female C57BL/6 mouse model for asthma to determine the role of PTEN in allergen-induced airway inflammation, specifically in the expression of VEGF. Allergen-induced airway inflammation leads to increased activity of PI3K in lung tissue. These mice develop the following typical pathophysiological features of asthma in the lungs: increased numbers of inflammatory cells of the airways; airway hyper-responsiveness; increased expression of interleukin (IL)-4, IL-5, IL-13, intercellular adhesion molecule 1, vascular cell adhesion molecule 1, regulated on activation normal T cell expressed and secreted (RANTES), and eotaxin; increased vascular permeability; and increased levels of VEGF. Administration of PI3K inhibitors or adenoviruses carrying PTEN cDNA reduced the symptoms of asthma and decreased the increased levels of plasma extravasation and VEGF in allergen-induced asthmatic lungs. These results indicate that PTEN reduces VEGF expression in allergen-induced airway inflammation.</P>
Lee, Kyung Sun,Kim, So Ri,Park, Seoung Ju,Park, Hee Sun,Min, Kyung Hoon,Jin, Sun Mi,Lee, Moon Kyu,Kim, Uh Hyun,Lee, Yong Chul Elsevier 2006 The journal of allergy and clinical immunology Vol.118 No.1
<P><B>Background</B></P><P>Reactive oxygen species (ROSs) play a crucial role in the pathogenesis of airway inflammation. Peroxisome proliferator activated receptor (PPAR)-γ is also involved in airway inflammation. We have demonstrated that the administration of PPARγ agonists or adenovirus carrying PPARγ cDNA (AdPPARγ) reduced bronchial inflammation and airway hyperresponsiveness. However, the effects of PPARγ on ROS generation in conditions associated with airway inflammation have not been clarified.</P><P><B>Objective</B></P><P>This study aimed to investigate the effects of the PPARγ on ROS generation in allergic airway disease of mice.</P><P><B>Methods</B></P><P>We have used a female C57BL/6 mouse model for allergic airway disease to determine the role of PPARγ.</P><P><B>Results</B></P><P>In this study with an ovalbumin-induced murine model of allergic airway disease, the increased ROS generation and the increased expression of T<SUB>H</SUB>2 cell cytokines, adhesion molecules, chemokines, and vascular endothelial growth factor in lungs after ovalbumin inhalation were significantly reduced by the administration of PPARγ agonists or AdPPARγ. We also showed that the increased nuclear factor-κB and hypoxia-inducible factor 1α levels in nuclear protein extracts of lung tissues after ovalbumin inhalation were decreased by the administration of PPARγ agonists or AdPPARγ.</P><P><B>Conclusion</B></P><P>These results indicate that the effects of PPARγ are mediated by the modulation of ROS generation and activation of redox-sensitive transcription factor nuclear factor-κB and HIF-1α in allergic airway disease of mice.</P><P><B>Clinical implications</B></P><P>Thus, these findings provide a pivotal molecular mechanism for the use of PPARγ agonists to prevent and/or treat asthma and other airway inflammatory disorders.</P>
Antioxidant Down-Regulates Interleukin-18 Expression in Asthma
Lee, Kyung Sun,Kim, So Ri,Park, Seoung Ju,Min, Kyung Hoon,Lee, Ka Young,Jin, Sun Mi,Yoo, Wan Hee,Lee, Yong Chul American Society for PharmacologyExperimental Ther 2006 Molecular pharmacology Vol.70 No.4
<P>An alteration in the balance between a T-helper type 2 cell (Th2) response and a Th1 response may predispose to the development of bronchial asthma. Interleukin-18 (IL-18) has an ability to promote both Th1 and Th2 responses, depending on the surrounding cytokine environment. Reactive oxygen species (ROS) play a crucial role in the pathogenesis of airway inflammation and hyperresponsiveness. Recent studies have demonstrated that antioxidants are able to reduce airway inflammation and hyperreactivity in animal models of asthma. In this study, we used a C57BL/6 mouse model of allergic asthma to examine the effects of antioxidants on the regulation of IL-18 expression. Our present study with ovalbumin-induced murine model of asthma revealed that ROS production in cells from bronchoalveolar lavage fluids was increased and that administration of L-2-oxothiazolidine-4-carboxylic acid or alpha-lipoic acid reduced the increased levels of ROS, the increased expression of IL-18 protein and mRNA, airway inflammation, and bronchial hyperresponsiveness. Our results also showed that antioxidants down-regulated a transcription factor, nuclear factor-kappaB (NF-kappaB), activity. These results indicate that antioxidants may reduce IL-18 expression in asthma by inhibiting the activity of NF-kappaB and suggest that ROS regulate the IL-18 expression.</P>
Lee, Min Ju,Suh, Chae Ri,Shin, Jeong Hee,Lee, Jee Hyun,Lee, Yoon,Eun, Baik-Lin,Yoo, Kee Hwan,Shim, Jung Ok The Korean Society of Pediatric Gastroenterology 2019 Pediatric gastroenterology, hepatology & nutrition Vol.22 No.6
Arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome is a rare autosomal recessive multisystemic disease that is associated with the liver, kidney, skin, and central nervous and musculoskeletal systems. ARC occurs as a result of mutations in the VPS33B (Vacuolar protein sorting 33 homolog B) or VIPAR (VPS33B interacting protein, apical-basolateral polarity regulator) genes. A female infant presented with neonatal cholestasis with a severe clinical outcome. She was diagnosed with ARC syndrome using targeted exome sequencing (TES). Exome sequencing revealed compound heterozygous mutations, c.707A>T and c.239+5G>A, in VPS33B, where c.707A>T was a novel variant; the resultant functional protein defects were predicted via in silico analysis. c.239+5G>A, a pathogenic mutation that affects splicing, is found in less than 0.1% of the general population. Invasive techniques, such as liver biopsies, did not contribute to a differential diagnosis of ARC syndrome; thus, early TES together with clinical presentations constituted an apparently accurate diagnostic procedure.
Quality Improvement of Pork Loin by Dry Aging
Lee, Cheol Woo,Lee, Ju Ri,Kim, Min Kyu,Jo, Cheorun,Lee, Kyung Haeng,You, Insin,Jung, Samooel Korean Society for Food Science of Animal Resource 2016 한국축산식품학회지 Vol.36 No.3
This study aimed to investigate the effects of dry aging on the quality of pork loin. Longissimus lumborum muscles were dissected from the right half of five pork carcasses and were used as the control samples. The left halves of the carcasses were aged at 2±1℃ and a relative humidity of 80% for 40 d. The total aerobic bacteria count was similar between the control and dry-aged pork loin (p>0.05). Lactic-acid bacteria was absent in both the control and dry-aged pork loins. Dry-aged pork loin contained low moisture and high protein and ash compared to the controls (p<0.05). The pH was higher and cooking loss was lower in dry-aged pork loin compared to that in the control (p<0.05). Flavor related compounds, such as total free amino acid, hypoxanthine, and inosine of pork loin were higher in dry-aged pork loin; whereas, inosine 5'-monophosphate and guanosine 5'-monophosphate were low in dry-aged pork loin than control (p<0.05). There was no difference in carnosine and anserine content between dry-aged pork loin and the control (p>0.05). Dry-aged pork loin had lower hardness and shear force and received higher core in sensory evaluation than the control (p<0.05). According to the results, dry aging improved textural and sensorial quality of pork loin.