Effects of Abeliophyllum distichum Nakai Flower Extracts on Antioxidative Activities and Inhibition of DNA Damage

Ahn, Joungjwa,Park, Jae Ho The Plant Resources Society of Korea 2013 한국자원식물학회지 Vol.26 No.3

The present study attempts to evaluate antioxidant activities of extracts from Abeliophyllum distichum. Nakai flower. The samples were collected in Jangyyeon-myeon, Goesan-gun, Korea and extracted with either hot-water or ethyl acetate (EtOAC). In DPPH, hydroxyl radical scavenging activity and $Fe^{2+}$ chelating activity of EtOAC extracts were 93.41%, 98.43%, and 7.38%, while those of hot-water extracts were 86.93%, 41.33% and 47.68% at 200 ${\mu}g/ml$, respectively. In ${\varphi}X$-174 RF I plasmid DNA cleavage assay, the protective effects of EtOAC and hot-water extracts against oxidative DNA damage were 82% and 17% at 200 ${\mu}g/ml$, respectively. Both extracts showed the protective effect of DNA migration by oxidative stress in intracellular DNA migration assay. Both extracts had no cytotoxity in NIH3T3 cells. Several polyphenolic compounds were identified such as 2-methoxy-benzoic acid, vanillic acid, phytol and pulegone by GC/MS. These results indicated that extracts of Abeliophyllum distichum Nakai flower showed antioxidant activities and protective activities against oxidative DNA damage and showed the possibility to be used as an effective natural antioxidants.

Physicochemical, Microbial, and Sensory Properties of Yogurt with Ulmus davidiana var. japonica During Storage

Ahn, Joungjwa(안정좌) 한국지역사회생활과학회 2014 한국지역사회생활과학회지 Vol.25 No.4

An Aqueous Extract of a Bifidobacterium Species Induces Apoptosis and Inhibits Invasiveness of Non-Small Cell Lung Cancer Cells

Joungjwa Ahn,김혜성,양경미 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.6

Chemotherapy regimens for non–small cell lung cancer (NSCLC) have various adverse effects on the human body. For this reason, probiotics have received attention regarding their potential value as a safe and natural complementary strategy for cancer prevention. This study analyzed the anticancer effects of aqueous extracts of probiotic bacteria Bifidobacterium bifidum (BB), Bifidobacterium longum (BL), Bifidobacterium lactis (BLA), Bifidobacterium infantis 1 (BI1), and Bifidobacterium infantis 2 (BI2) on NSCLC cell lines. When the aqueous extracts of probiotic Bifidobacterium species were applied to the NSCLC cell lines A549, H1299, and HCC827, cell death increased considerably; in particular, the aqueous extracts from BB and BLA markedly reduced cell proliferation. p38 phosphorylation induced by BB aqueous extract increased the expression of cleaved caspase 3 and cleaved poly (ADP-ribose) polymerase (PARP), consequently inducing the apoptosis of A549 and H1299 cells. When the p38 inhibitor SB203580 was applied, phosphorylation of p38 decreased, and the expression of cleaved caspase 3 and cleaved PARP was also inhibited, resulting in a reduction of cell death. In addition, BB aqueous extracts reduced the secretion of MMP-9, leading to inhibition of cancer cell invasion. By contrast, after transfection of short hairpin RNA shMMP-9 (for a knockdown of MMP-9) into cancer cells, BB aqueous extracts treatment failed to suppress the cancer cell invasiveness. According to our results about their anticancer effects on NSCLC, probiotics consisting of Bifidobacterium species may be useful as adjunctive anticancer treatment in the future.

ω‑hydroxyundec‑9‑enoic acid induction of breast cancer cells apoptosis through generation of mitochondrial ROS and phosphorylation of AMPK

Joungjwa Ahn,Hyesung Kim,Kyung Mi Yang 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.7

This study was performed to evaluate the anticancereffect of ω-hydroxyundec-9-enoic acid (ω-HUA), amicrobial bio-catalyst product in breast cancer cells, throughAMP-activated protein kinase (AMPK) regulation. ω-HUAmediated apoptosis was induced in breast cancer cells byAMPK activation, loss of mitochondrial membrane potential,and reactive oxygen species (ROS) generation. ω-HUAtreatment of breast cancer cells increased the AMPK phosphorylationlevels, cleaved caspase-3, and poly (ADP-ribose)polymerase (PARP) proteins. In addition, anti-apoptoticmembers, such as Bcl-2, were downregulated, while Bax, apro-apoptotic member, was upregulated. ω-HUA decreasedthe mitochondrial membrane potential while increasing theexpression of cytochrome c (cyt c). Treating the cells withcompound C, an AMPK inhibitor, reversed the phenomena,leading to an increase in cell viability and a decrease inapoptosis induction. Treating the cells with an ROS scavenger,N-acetyl cysteine (NAC), led to AMPK inactivation andapoptosis inhibition, allowing the recovery of cell health. In conclusion, ω-HUA sequentially caused the productionof mitochondrial ROS and the consequent AMPK activation,thereby inducing apoptosis in breast cancer cells. Thus,ω-HUA may prove useful as an anticancer agent that targetsAMPK in breast cancer cells.

Properties of cholesterol-reduced ice cream made with cross-linked &bgr;-cyclodextrin

HA, HYUN-JEE,AHN, JOUNGJWA,MIN, SANG-GI,KWAK, HAE-SOO Blackwell Publishing Ltd 2009 International journal of dairy technology Vol.62 No.3

<P>This study examined the properties of cholesterol-reduced ice cream stored at −12, −18 and −28°C. Over 90.0% cholesterol was removed, and ice crystal size decreased while viscosity increased as the freezing temperature decreased in the cholesterol-reduced ice cream. The melting time was slightly shorter in the cholesterol-reduced ice cream than in the control. A significant difference was found in the dull colour, and crumbly and unnatural flavour, especially when stored at −28°C, regardless of cross-linked &bgr;-cyclodextrin treatment. This study indicated that although some differences were observed, most of the properties of the cholesterol-reduced ice cream were comparable to the control ice cream.</P>

‘식품산업과 영양’ 학회지에서 사용된 통계방법에 대한 고찰

명성민(Sungmin Myoung),안정좌(Joungjwa Ahn) 한국식품영양과학회 2018 식품산업과 영양 Vol.23 No.1

Antioxidant Activities of Powdered and Ultra-fine Powdered Ulmus Davidiana var. Japonica

Jae Ho Park(박재호),Joungjwa Ahn(안정좌) 한국지역사회생활과학회 2016 한국지역사회생활과학회지 Vol.27 No.3

Effect of Isoflavone-Enriched Milk on Bone Mass in Ovariectomized Rats

Byung-Ju Jeon,Joungjwa Ahn,Hae-Soo Kwak 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.6

This study examined the effect of isoflavone-enriched milk on bone loss in ovariectomized (OVX) rats. Thirty 6-week-old Sprague-Dawley female rats were divided into two groups: sham-operated and OVX. The OVX group was subdivided into three dietary groups (OVX, non–isoflavone-enriched milk; OVX+Iso, isoflavone-enriched milk; and OVX+Iso+Vit+Ca, isoflavone-, vitamins D and K-, and Ca-enriched mik). After 19 weeks of feeding, the food efficiency ratio and body weight gain in the sham-operated group were significantly lower compared with those in the other groups. The bone alkaline phosphatase and total alkaline phosphatase activities were significantly higher in isoflavone-enriched groups (OVX+Iso and OVX+Iso+Vit+Ca) when compared with the sham-operated group. Urinary excretions of deoxypyridinoline and hydroxyproline were significantly higher with ovariectomy, but mostly normalized in the OVX+Iso and OVX+Iso+Vit+Ca groups. The rats in the OVX+Iso and OVX+Iso+Vit+Ca groups showed higher femur and tibia weights. A significant increase was found in bone density of femur and trabecular bone area in the OVX+Iso+Vit+Ca group, which almost reached that of the sham-operated group, whereas no difference was found among the OVX and OVX+Iso groups. The histological areas of the proximal tibia sections showed highly filled trabecular bone in both isoflavone-enriched groups (OVX+Iso and OVX+Iso+Vit+Ca). The present study indicated that isoflavone-enriched milk may have a partial preventive effect on ovariectomy-induced bone loss; however, vitamins D and K and Ca enrichment with isoflavone may enhance effectiveness for increasing bone mass in OVX rats.

Effect of Nano-Calcium-Enriched Milk on Calcium Metabolism in Ovariectomized Rats

Heung-Sik Park,Joungjwa Ahn,Hae-Soo Kwak 한국식품영양과학회 2008 Journal of medicinal food Vol.11 No.3

This study was designed to examine the effect of different kinds of calcium enrichment on serum and urine indices of mineral status in ovariectomized rats. Twenty-four 7-week-old Sprague-Dawley female rats were divided into four groups, ovariectomized, and fed diets containing the following: (1) Control, non–Ca-enriched milk; (2) OVX1, calcium carbonate-enriched milk; (3) OVX2, ionized Ca-enriched milk; and (4) OVX3, nano-Ca-enriched milk. After 18 weeks of feeding, the food efficiency ratio in the nano-Ca-fed group was significantly lower compared with those in the Control and OVX2 groups. There was no difference in serum and fecal Ca among the groups. The bone/total alkaline phosphatase ratio was significantly higher in rats fed milk enriched with nano-Ca (59%) and calcium carbonate (62%) than in control (44%) animals. Urinary Ca was the highest in the nano-Ca-enriched group; however, urinary excretions of deoxypyridinoline and hydroxyproline were significantly decreased in the nano-Ca-enriched group. The present results indicate that consumption of nano-Ca-enriched milk resulted in an increase of urinary excretion of calcium and a decrease in deoxypyridinoline and hydroxyproline in ovariectomized rats.

Optimizing cholesterol removal from cream using &bgr;-cyclodextrin cross-linked with adipic acid

HAN, EUN-MI,KIM, SONG-HEE,AHN, JOUNGJWA,KWAK, HAE-SOO Blackwell Publishing Ltd 2007 International journal of dairy technology Vol.60 No.1

<P><I>The present study was carried out to optimize the different conditions for cross-linked &bgr;-cyclodextrin (&bgr;-CD) using adipic acid on cholesterol removal in cream. Different factors were &bgr;-CD concentration, mixing temperature, mixing time and mixing speed. Cross-linked &bgr;-CD was prepared with adipic acid. When the cream was treated with 10% cross-linked &bgr;-CD, cholesterol removal was the highest at 90.7%, which was not significantly different from treatments with 15% and 20% cross-linked &bgr;-CD. Cholesterol removal was significantly influenced by mixing temperature, mixing time and mixing speed. After cholesterol removal from cream, the used cross-linked &bgr;-CD was washed for cholesterol dissociation and reused. For recycling study, the cholesterol removal in first trial was 91.5%, which was mostly same as that using new cross-linked &bgr;-CD. However, after 10 repeated trials using the same sample, only 83.4% of the cholesterol was removed from cream. Therefore, the present study indicated that the optimum conditions for cholesterol removal using cross-linked &bgr;-CD were 10%&bgr;-CD addition, 30 min mixing with 1400 r.p.m. speed at 40°C with over 90% cholesterol removal. In addition, recycled &bgr;-CD cross-linked with adipic acid showed a similarly high recycling efficiency to unused &bgr;-CD of about 90% up to the seventh trial.</I></P>