감초 내 글라브리딘의 Semi-pilot 규모 초임계유체 추출

임교빈,유종훈 수원대학교 산업기술연구소 2004 산업기술연구소논문집 Vol.19 No.-

The purpose of this work was to study the feasibility of a semi-pilot scale supercritical CO₂(SCCO₂) extraction of glabridin from liquorice. The extraction experiments were carried out at temperatures ranging from 40 to 60℃ and at pressures ranging from 100 to 300 bar. The effects of process variables such as the type and amount of cosolvents, temperature, and pressure on the extraction yield, chemical composition of the extract, and extraction kinetics were investigated in detail. The chemical compositions of the extract were analyzed by high-performance liquid chromatography. Almost no glabridin was extracted at all extraction conditions when only pure supercritical carbon dioxide was employed as an extraction solvent, whereas a significant amount of glabridin was extracted in the presence of cosolvents such as acetone and ethanol. The experimental results obtained in this study revealed that the extraction yield not only increases but the extraction time also decreases as the concentration of cosolvents in SCCO₂ increases up to about 25%. Ethanol was found to be more effective in SCCO₂extraction of glabridin than acetone.

競技種目別 運動選手들의 筋力에 관한 硏究

박철빈,송종국,박수연,박찬호 경희대학교 체육과학연구소 1996 體育學論文集 Vol.24 No.-

The purpose of this study was to estimate physique and muscular strength in collegiate athletes. On a sample of 70 athletes (basketball, baseball, soccer, and ice-hockey) anthropometric measurements (height, weight, bone width, circumference and skinfold thicknesses) were taken, For the measurements of muscular strength grip strength, back strength, arm flexion and leg extension strength were tested. The results of this study showed that basketball players were highest for height, weight, humorous and femur diameters, chest circumference, and calf circumference. Ice-hockey players were highest for upper arm and flexed upper arm circumferences. On the other hand ice-hockey players were lowest for height, soccer players for weight, baseball players for humerous and femur diameters, soccer players for chest circumference. For skinfold thickness baseball players (10.1mm) were highest for triceps, basketball players (14.5mm) for subscapular, ice-hockey players (13.3mm) for suprailiac and medial calf (9.0mm) skinfold thicknesses. While soccer players were lowest for triceps (8.7mm), subscapular (8.6mm), suprailiac (9.8mm) and medial calf (7.5mm) skinfold thicknesses. There were significant differences (P〈0.01) for height, weight, femur diameter, upper arm and flexed upper arm circumferences among athletes. Grip strength (left and right) was highest in baseball players (52.1kg, 50.8kg) but lowest for soccer players (42.3kg, 42.8kg). Baseball players were highest for back strength (162.0kg). On the other hand back strength was lowest in ice-hockey players (127.1kg). For arm flexion and leg extension strength (left, right) basketball players (42.0kg, 44.9kg) were highest for both strength tests. While soccer players were lowest for arm flexion strength and baseball players for leg extension strength. Ice-hockey players were highest for sit-up (59.2). Significant differences (P〈0.01) were found for grip, back, arm flexion and leg extension strength and sit-up among athletes.

임계압력 근처에서 오일 에멀젼의 정밀여과 특성에 관한 실험 연구

유종훈,임교빈 수원대학교 산업기술연구소 2003 산업기술연구소논문집 Vol.18 No.-

In the present study the membrane filtration mechanism of an oil-in-water (O/W) emulsion through two kinds of straight-pore microfiltration membranes with average pore sizes of 0.2㎛ and 0.4 ㎛, respectively, has been investigated. An emulsion-type metalworking fluid has been permeated through these two membranes using a specially designed microfiltration apparatus. The theory on the critical pressure proposed by Nazzal and Wiesner railed to account for the experimental results obtained in this work. Our results revealed that O/W emulsions undergo a phase inversion to W/O emulsions above a certain concentration as the concentration of oil in the O/W emulsion increases near and on the membrane surface. A further increase in the transmembrane pressure resulted in the breakdown of the oil film formed on the membrane surface and, consequently, an abrupt increase in the permeate flux. Therefore, it has been suggested that the critical pressure should be redefined as the transmembrane pressure at which the oil film formed on the membrane surface starts to break down.

상악 정중과잉치의 발병율

김종빈,김종수,박지은 大韓小兒齒科學會 2007 大韓小兒齒科學會誌 Vol.34 No.4

2003년 4월부터 2006년 4월까지 안양 지역에 위치한 개인 소아치과 병원에 내원한 환아 7038명을 대상으로 임상적,방사선학적 검사를 통해 성별,상악 정중과잉치의 수,치관의 형태와 위치,치관 방향을 조사하여 다음과 같은 결론을 얻었다. 1. 과잉치를 가진 환아는 239명으로 3.40%(총 241명. 3.42%)에 해당하였고 총 289개의 과잉치가 있었다. 2. 과잉치는 1개 있는 경우가 191명으로 가장 많았고, 2개 있는 경우도 48명 있었으며 3개 이상 있는 경우는 없었다. 3. 상악 정중과잉치는 3.2:1로 여아보다 남아에서 호발하였다. 4. 과잉치는 왼쪽에 있는 경우가 55.4%로 약간 더 많았고,모양은 원추형,방향은 역위형이 가장 많았다. 5. 하악 정중과잉치는 2명에서 관찰되었다. The purpose of this study was to evaluate the prevalence of mesiodens at pediatric dental clinic in Anyang, Kyeong Gi-Do. From retrospective reviews of all patients who visited Kizwell pediatric dental clinic from 2003.4-2006.4, 241 patients (289 mesiodens) who were shown to have mesiodentes were examed by a periapical radiograph and a panoramic radiograph. The incidence of mesiodens was 3.42% and the ratio of male/female (184/57) was 3.2:1. The number of supernumerary teeth was one in 193 cases (80%), two in 48 cases (20%). The mesiodens was placed at left side more frequently and the most common shape was conical type (208, 78%). Of 289 mesiodens, the direction of the crown of the mesiodens was inverted in 156 (54%), in a normal direction in 104 (36%), in a horizontal direction with regard to the tooth axis in 29 (10%). Early diagnosis of the mesiodens allow the most appropriate treatment, often reducing the extent of surgery, orthodontic treatment and possible complications.

인공위성 본체 추진 모듈의 최적화

임종빈,박정선 한국 항공대학교 항공산업기술연구소 2001 航空宇宙産業技術硏究所 硏究誌 Vol.11 No.-

본 연구에서는 인공위성 추진모듈에 대하여 최적화를 수행하였다. 추진모듈의 최적화는 발사환경을 고려하여 제한조건과 하중 조건을 설정하였다. 또한, 보편적인 최적화 프로그램에서는 다루기 힘든 제한 조건을 고려하기 위한 방법을 모색하였다. 이 방법은 유한요소 해석 프로그램과 최적화 프로그램을 연결하여 최적화를 수행하는 것이다. 이 방법에 의하여 유전자 알고리즘을 이용한 추진모듈의 최적화를 수행하였고, 유용한 정보를 얻었다. 본 연구의 결과 인공위성 구조인 추진모듈의 최적화에서는 응력에 의한 조건보다는 고유진동수에 의한 제한조건이 최적화 수행에 큰 영향을 미침을 알 수 있었다. A portion of a satellite structure is considered to optimize. The optimization approach provides a way to handle constraints under severe space environments and to combine optimization programs with commercial analysis codes. Optimization techniques, GA(genetic algorithm), is used with commercial finite element analysis far the optimization of satellite structure. Constraints are several stresses for static analysis and natural frequency for dynamic analysis. From numerical optimization results, it is found that the GA may reach the optimum design. Natural frequency are more active constraints in the optimization procedures in this study.

초임계 이산화탄소를 이용한 웨이퍼 세정

임교빈,유종훈 수원대학교 산업기술연구소 2003 산업기술연구소논문집 Vol.18 No.-

Organic and aqueous solvents are largely used in cleaning processes for semiconductor manufacturing. In this study, we examined a non-plasma, environmentally benign stripping method using supercritical carbon dioxide (SCCO₂) to remove ion implanted photoresist and residues from a wafer surface at temperatures ranging from 80 to 200℃ and at pressures from 150 to 400 bar. Polar cosolvents were added to nonpolar SCCO₂ to improve the stripping efficiency of photoresist and residues. A suggested cleaning mechanism is swelling and plasticization of the polymer by SCCO₂ associated with degradation by the cosolvents, thereby lowering molecular weight and increasing solubility. The I-line photoresist whose chemical structures had been changed after ion implantation was not easily removed by using SCCO₂ containing single cosolvent. However, SCCO₂ containing multiple cosolvents showed a high stripping efficiency of carbonized photoresist (about 90%) under the operating conditions of 80~100℃ and 150~250 bar. The experimental results obtained in this study verified that the SCCO₂ cleaning method is a promising alternative to traditional photoresist wet-stripping techniques.

한국인에서 DXS7132 유전좌와 GATA31D10 유전좌의 다형성에 관한 연구

안종성,장영길,이숭덕,신창호,이윤성,이정빈 大韓法醫學會 2000 대한법의학회지 Vol.24 No.1

The validation study for two STR loci on X-chromosome, DXS7132 and GATA31D10, was done including allelic distribution and frequency of each allele to use these results for individual identification and paternity testing. For 496 unrelated Koreans, above two STR loci were amplified simultaneously using duplex PCR amplification method. The amplified products were analyzed by polyarylamide gel electrophoresis followed by silver staining. In male DXS7132 locus revealed 7 different alleles ranging from 276bp to 300bp. The largest allele was consisted of 14 repetition of [TCTA] unit and took 0.3417. The allele 15 followed next as 0.3165 and allele 13 as 0.1726. In female general distribution was same except one allele, allele 18 was found additionally. The heterozygosity was 0.7706 and 23 different genotypes were found. Polymorphism information content(PIC) was 0.727. Two cases of mutation were noted in DXS7132 locus In both male and femal 7 different alleles were noted in GATA31D10 locus and the alleles ranged from 195bp to 231bp. The allele 15(199bp) took the majority of all as 0.825. The other alleles showed rather relatively low frequency. The heterozygosity was 0.2385 and 11 different genotypes were found. PIC was 0.2521, and no mutation was noted in GATA31D10 locus. Considering these two loci together, 22 different halpotype were noted.

One-Bottle 상아질 결합제의 유치 상아질에 대한 전단 결합 강도에 관한 연구

김종빈,김종수 대한소아치과학회 2000 大韓小兒齒科學會誌 Vol.27 No.3

치아 교환시기에 발거된 치관이 건전한 유구치 상아질 표면을 One-Bottle 상아질 결합체인 Primer & Bond NT(De Trey/Dentsply, Konstanz, Germany)와 Single Bond(3M Co. U.S.A.) 그리고 대조군으로 Scotchbond Multipurpose System(3M Co. U.S.A.)를 이용하여 각 제조자의 지침에 따라 처리하여 레진을 충전하고 광중합하였다. 1.전단 결합 강도의 측정결과 Primer & Bond NT군인 1군, Single Bond군인 2군, 그리고 대조군으로 사용된 Scotchbond Multipurpose System군인 3군의 순서로 1군에서 다소 높은 전단 결합 강도를 보였으나, 모든 군들간에는 통계적 유의차가 없었다(p〉.05). 2.주사 전자 현미경 소견에서 1군 2군에서 서로 유사한 양상의 잘 형성된 혼화층이 관찰되었으며, 레진 테그와 측방 가지 또한 다수 관찰되었다. 반면 3군에서는 혼화층이나 측방 가지의 형성이 1,2군에 비해 다소 미약한 양상을 보였다. 주요단어 : One-Bottle 상아질 결합체, 유구치 상아질, 전단 결합 강도, 주사 전자 현미경 To test the shear bond strength of a new "one-bottle adhesive" system to primary dentin two commercially available one-bottle adhesives(Prime & Bond NT, Single bond) and conventional three step system(Scotchbond Multi-Purpose Plus) were included for comparison. And We observe the interfacial morphology by scanning electron microscope. 90 primary molar teeth were embedded in acrylic and buccal and lingual surface were polished to 320 grit to create standardized dentin surface for testing. After bonding of composite resin to sample surfaces according to the manufacturer's direction and 1000 times thermocycling in dwell time 30 second, Shear bond strengths of adhesives to dentin were determined using universal testing machine and analyzed by ANOVA test. Another groups of specimens were treated by hydrochloric acid to secure the resin only and those tags were evaluated under SEM fur their length and forms and the morphology of the bonding sites were also observed The result are as follows 1 Group I(Prime & Bond NT) showed higher shear bond strength than groupⅡ(Single Bond) and Ⅲ(Scotchbond Multi Purpose Plus) but no statistically significant difference was founded between groups(p〉0.05). 2. Relating long resin tags of 70-120㎛ were observed in samples of all groups under SEM. We could observed hybrid layer, resin tag and many lateral branches in every group. But, we observed in groupⅢ rare lateral branched than other two group and discontinuous hybrid layer.

국내에서 채집한 진드기에서 중합효소연쇄반응을 이용한 라임병균 및 Ehrlichiosis 원인체의 검출

김종배,엄용빈,박성언,박상욱,김영미,송혜원,안준환 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1998 Journal of biomedical laboratory sciences Vol.4 No.2

국내에서 채집한 진드기의 라임병 및 ehrlichiosis원인체 보균 상태를 조사하기 위하여 총 516마리 (Ixodes spp. 22마리, Haemaphysalis spp. 494마리)의 ixodid 진드기를 봄과 가을에 걸쳐 강원도 고산지대 일원에서 채집하였다. 수집한 진드기에서 DNA를 추출 ·정제한 후 추출한 DNA를 template로 이용하여 , Borrelia burgdorferi sensu lato 및 Ehrlichia spp.에 특이하게 반응하도록 제작한 primer를 이용한 중합효소연쇄반응 (polymerase chain reaction, PCR)을 실시하였으며, 이 결과를 oligonucleotide probe를 사용한 southern blotting을 통하여 다시 확인하였다. 총 516마리의 진드기중 B.garinii sensu lato DNA 양성 인 진드기는 68(13.2%)마리 (B. burgdorferi sensu stricto,2; B. afzelii,1; B. garinii,33; B.tanukii,8; B.turdae, 4)로 나타났으며 이 중 37 (7.2%)마리의 진드기 는 southern blot analysis에서도 양성으로 확인되었다. 또한 101 (19.2%)마리의 진드기가 Ehrlichia spp.에 대한 PCR에서 양성 이었으나, 이들 중 25 (4.8%)마리 만이 southern blot analysis에서 양성으로 확인되었다. 그러나 사람의 병인체로 추정 되는 human granulocytic ehrlichiosis (HGE) agent DNA를 보균한 진드기는 확인되지 않았다. 한편 라임병균과 ehrlichiosis원인체를 동시에 보균한 것으로 밝혀진 진드기가 3마리에서 (0.6%)확인되어,국내에서도 진드기 교상시 이들 두 가지 열성질환이 동시에 감염될 가능성이 있는 것으로 사료됨으로 진드기 매개성 열성질환에 대한 적절한 진단법 등을 보다 체계적으로 연구하여야 할 것으로 판단된다. To investigate the distribution of Borrelia Burgdorferi and human granulocytic ehrlichiosis(HGE) agent in ticks, adult ixodid ticks of Ixodes spp. and Haemaphysalis spp. were collected from the high mountain areas of Kangwon Province. Using DNAs extracted and purified in the collected ticks, polymerase chain reaction (PCR) was performed to amplify the specific nucleotide sequences of both agents. Of the 516 ticks, a total of 68 (13.2%) ticks was positive for Borrelia burgdorferi sensu lato with PCR analysis (2 for B. burgdorferi sensu stricto; 1 for B. afzelii; 33 for B. garinii; 8 for B.tanukii; 4 for B. turdae). However a little more than half of PCR-positive ticks (37/68) was found to be positive in the southern blot analysis with Bl6S oligonucleotide probe. One hundred and one (19.2%) ticks were positive for Ehrlichia spp. in PCR, and a quarter of them (25/101) was positive in southern blot with E16S oligonucleotide probe. But none of them was found to be the DNA of HGE agent. And 0.6% (3/516) ticks were positive for both of B. burgdorferi sensu lato and Ehrlichia spp. These findings might implicate the possibility of the outbreak of lyme borreliosis and ehrlichiosis in Korea, and more extensive studies may be need for the diagnosis of multiple tick-borne diseases.

거대괴 착이온(NiL²+)의 낮은 스핀상태(D4h)와 높은 스핀상태(Oh)간의 평형

卞鐘轍,韓性彬 濟州大學校 基礎科學硏究所 1990 基礎科學硏究 Vol.3 No.1

물, 아세토니트릴, 아세톤 및 니트로메탄에서 Ni(II)-tetraamine 착물의 화학평형을 분광광도법을 이용하여 각각 관찰하였다. Ni(II)-tetraamine 착이온의 낮은 스핀(??)구조와 높은 스핀(??) 구조간의 평형이 나트로메탄을 제외한 물, 아세토니트릴, 아세톤 용액에서 나타났다. 각 용액에서 온도와 [NaClO₄]가 증가 할수록 이 반응의 평형상수는 감소하였다. 각 용매에서 반응엔탈피와 반응엔트로피는 착물의 전자 스펙트럼에 대한 온도의 영향을 분석하므로써 결정하였다. ?? 구조인 triplet 화학종의 형성시, 아세토니트릴과 아세톤 용액에서 반응엔탈피(??)와 반응엔트로피(??)는 각각 1.26, 1.32 그리고 25.01, 54.90이었다. The chemical equilibria of Ni (II)-tetraamine (tetraamine=L, 2, 12-dimethyl-3, 7, 11, 17-tetraazabicyclo-11, 3, 1-heptadeca-1(17), 2, 11, 13, 15-pentaene) complex ion in water, acetonitrile, acetone and nitromethane were investigated using spectrophotometric method, respectively. The equilibria between low-spin(??) and high-spin(??) structures of Ni=tetraamine complex ion were presented in water, acetonitrile and acetone, but not in nitromethane. The equilibrium constants, the reaction enthalpies and the reaction entropies were determined from analysis of the temperature dependence of the electronic spectra. The formation of the triplet species (??) was found to be exothermic. The solvent and electrolyte effects on the equilibrium constants could be explained by the dielectric constants of solvents and the reaction entropies.