합성수지 충전물을 이용한 충전탑에서 암모니아 처리에 관한 연구

김장호,김형호,김종수,김재홍,이경재 동아대학교 환경문제연구소 2003 硏究報告 Vol.25 No.2

In this study, packed column was chosen so as to remove ammonia gases, kinds of odor generated from industrial fields. Random packing 35mm Pallring pp., that is suitable for the environmental protection industry, was used because the packings are comparatively simple and easy to manufacture. Random packings 35mm Pallring, PP. has a form of lattice as plastic(po1ypropylene). By studying the characteristics for packing by experiment of dry pressure drop, hydraulic pressure drop, liquid hold-up and mass transfer, packed column is made as 300 [mm] in diameter, 1400 [mm] in height, the applicable criteria to the noxious gas treatment process was presented as follows.

일부 의료서비스업 VDT작업자의 상지 근골격계 증상의 정도와 관련된 요인

김형렬,원종욱,송재석,김현수,김치년,노재훈 大韓産業醫學會 2003 대한직업환경의학회지 Vol.15 No.2

목적: 본 연구는 상지 근골격계 질환의 유병율이 높은 집단으로 알려진 VDT작업자를 대상으로 이들의 근골격계 통증을 정량화하고, 정량화된 통증점수를 종속변수로 하여, 이와 관련성을 갖는 작업요인, 사회심리적 요인을 파악하고자 하였다. 방법: 서울지역에 위치한 1500병상 규모의 대학병원에 근무하고 단말기 작업을 주로 수행한다고 알려진 부서에 대해 병원내 안전관리과와 협의를 거쳐 163명을 연구대상으로 선정하였다. 이중 138명이 설문에 참가하였고, 이들을 최종 연구대상으로 하였다. 설문지를 가지고 연구변수를 측정하여, 나이, 근무연수, 가사노동의 정도 등의 인구학적 변수와 직무스트레스 및 사회적지지, 근골격계 증상, 통증의 양상 등을 조사하였다. 분석방법은 일반적 특성 및 작업특성, 통증의 양상을 성별에 따라 파악하였고, 통증점수의 타당성을 검정하기 위해, 통증점수의 구성요소와 통증점수를 시각적 상사척도와 상관성 검정을 시행하였다. 통증점수를 종속변수로 하고 직무요구도, 직무재량도, 동료의지지, 상사의지지, 여러작업환경특성, 인구학적 특징 등을 독립변수로 하여 다중회귀분석을 시행하였다. 결과: 첫째 통증의 정량화를 위해 사용한 통증평정지수는 시각적 상사척도와의 상관관계가 높은 것으로 나타나, 어느 정도의 타당성을 지닌 방법으로 해석할 수 있었다. 둘째, 이 통증 점수를 종속변수로 하여, 관련요인 분석한 다중회귀분석에서 직무요구도가 높을수록 통증의 정도가 높고(p<0.01), 여성일수록 남성에 비해 높은 통증점수를 보였다(p<0.01). 결론: 근골격계 질환의 높은 유병율을 보이는 집단의 경우, 이들의 증상 유무만을 가지고 관련요인을 분석하는 방법을 사용하기 어렵다. 따라서 이런 경우, 통증을 정량화하여, 다양한 관련요인을 찾으려는 노력이 필요하며, 본 연구에서 사용한 통증평정지수는 타당성을 지닌 정량화 방법이라고 여겨진다. 또한 본 연구에서는 직무요구도가 높을수록, 여성일수록 높은 통증점수를 보였다. Objectives: This study was designed to quantify symptoms in the upper extremities and to identify the pain related factors among hospital workers using video display terminals. Methods: The subject-group consisted of 138 employees working at a hospital using video display terminals. A structured questionnaire was used to estimate the participants' general characteristics, and job contents. Job stress was measured using Karaseks JCQ(Job Content Questionnaire). Pain in the upper extremities was identified according to the NIOSH symptoms survey criteria. Quantification was measured using Kim Chul's method. Pearson's Correlation analysis was used to identify validity in quantification. VAS(visual analog scale) was used to compare with this method. Multiple regression analyses elucidated the relationship between quantified pain and various work factors, such as job stress. Data were analysed with SAS 6.12 program. Results: First, in quantification using Kim Chul's method, the correlation between modified PRS(pain rating score) and VAS was extremely high by 0.60(P<0.01), indicating the high validity of PRS. Second, the groups of high job demand, high job control, and females were related to an increased pain level. Conclusions: These results suggest that the self pain assessment method, PRS, is valuable and useful in analyzing occupational musculoskeletal symptoms. High demand, job control and gender may influence the levels of pain. Prospective studies about musculoskeletal disorder are required.

석탄을 원료로한 활성탄 제조와 흡착특성에 관한 연구

최동훈,김형호,김종수,김재홍,김장호 동아대학교 환경문제연구소 2001 硏究報告 Vol.24 No.2

The purpose of this study is develop manufacture technology of activated carbon for purification of water, it is investigated suitable manufactures for manufacture of activated carbon for purification of water by use anthracite coal and bituminous coal produced in internal and external through this fact, economic valuation and made method established. it is valuated wether or not suitable high degree purification by know removal characteristic organic matter such as KMnO₄Consumption, DOC, UV-254, and THMFP make use of Pilot-plant (purification of water treatment) for made activated carbon. For mixed anthracite coal and bituminous coal by means of coal tar pitch, completely melt to fixed coal tar pitch apply l50℃ heat at stainless steel box, since then compounding weight rate 1:l of anthracite coal and bituminous coal sieved material, then add coal tar pitch and caking water, mixed suitable compounding rate since then extrude and mix by diameter 5.5m extruder, extruded mold cute and dry length l0mm pellet shape by cutter and then made activated carbon.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

심혈관계 질환으로 관상질환 집중치료실에 입원한 환자들의 혜후 측정을 위한 APACHEⅢ점수 체계의 임상적 유용성

이공섭,이창선,최현주,홍종서,강지향,김도형,이민영,오인균,김병엽,김상억,김학찬,한승혜,이재용,지영우,안경주 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.8

기어의 백래쉬를 고려한 승용차 조향계의 동특성 연구

김종관,김경석,송상기,정진형 한국공작기계학회 1996 한국생산제조학회지 Vol.5 No.3

The problem related to the rotational vibration at steering wheel end of passenger cars during high speed driving is investigated. To analyze vibration of steering wheel, a steering system of passenger car is modelled in twelve degrees of freedom including backlash effect of rack and pinion gear system. The one degree of freedom system with backlash in investigated by the analytical method . Consequently the skeleton curve and the frequency response curves are computed. The steering system is analyzed by the numerical simulation using the 4th order Runge-Kutta method, the obtained results are compared with the experimental data. Also the effects of the change of rack gear tooth stiffness and backlash on the acceleration level of steering wheel are investigated. As a result, it can be found that the acceleration level of steering wheel becames lower as the rack gear tooth stiffness becames higer, and that acceleration level becames high as the magnitude of backlash between rack and pinion gear increase.

중합효소연쇄 반응법에 의한 인형거대세포바이러스(human cytomegalovirus)의 신속한 검출

김의종,오명돈,박기호,신형식,이환종,김남중,최강원 대한감염학회 1996 감염 Vol.28 No.4

목 적 : 인형거대세포바이러스(HCMV)는 면역 저하 환자에서 폐렴, 망막염, 간염 등 치명적인 감염증의 원인이다. 전통적인 세포 배양법으로 HCMV를 분리하기까지는 1주에서 4주가 걸리므로 이 바이러스에 의한 질환이 의심되는 경우 신속한 진단법을 이용한 진단이 바람직하다. 저자들은 한국에서 분리되는 야생주 HCMV를 검출하는데 중합효소 연쇄 반응법을 이용할 경우의 진단적 유용성을 알기 위하여, 전통적인 세포배양법으로 HCMV가 분리된 검체를 이용하여 중합효소 연쇄 반응법의 특이도와 민감도를 평가하였다. 방 법 : 국내 야생주 HCMV 15주, 그리고 전통적인 바이러스 배양법으로 HCMV가 분리된 소변 15검체를 대상으로 중합효소 연쇄 반응을 실시하였다. 중합효소 연쇄 반응은 Towne주의 immediate early antigen의 유전자에서 유리된 primer MIE와 AD169주의 immediate early antigen의 유전자에서 유리된 primer IE를 이용하였다. 증폭 산물은 겔 전기영동 후 EtBR으로 염색하여 자외선 하에서 관찰하였다. Primer IE에 의한 증폭산물은 또한, DNA blot hybridization 방법으로 확인하였다. 결 과 : 1) 국내 HCMV 야생주 15주를 대상으로 중합효소 연쇄 반응을 한 결과 primer IE를 이용한 경우에는 100%(15/15)에서 177bp의 증폭 산물이 관찰되었고, 이들 증폭 산물은 모두 probe IE와 보합 결합되었다. primer MIE를 이용한 경우에는 93%(14/15)에서 435bp의 증폭 산물이 관찰되었다. 2) 세포 배양법으로 HCMV가 분리된 소변 15검체를 대상으로 primer IE를 이용하여 중합효소 연쇄 반응을 한 결과 direct gel analysis 법으로는 73%(11/15)에서, 보합 결합법으로는 87%(13/15)에서 관찰되었다. Primer MIE를 이용한 경우에는 direct gel analysis법으로 87%(13/15)에서 435bp의 증폭 산물이 검출되었다. 결 론 : Primer IE와 MIE를 이용한 중합효소 연쇄 반응법은 국내 야생주 HCMV를 신속히 검출하는데 유용한 검사법이다. Background : Human cytomegalovirus(HCMV) can cause pneumonitis, hepatitis, retinitis and other serious diseases in the immunocompromised patients. It takes 1 to 4 weeks to diagnose HCMV infection by conventional virus culture. Therefore, when HCMV diseases are suspected, a rapid diagnostic method such as polymerase chain reaction(PCR), antigen assay or shell vial culture is desirable. We evaluate the sensitivity and specificity of a PCR for the rapid detection of HCMV wild strains in Korea. Methods : We used 2 sets of primers ; primer IE and primer MIE derived from the sequence for immediate early gene of AD169 strain and Towne strain, respectively. Fifteen clinical isolates of HCMV, suspended in MRC-5 cells, were amplified by PCR. Fifteen urine specimens which were positive for HCMV by conventional virus culture were also amplified. Amplification products were analyzed by polyacrylamide gel electrophoresis. The products from PCR with primer IE were also identified by DNA blot hybridization. Results : PCR using primer IE gave the PCR products in all of the 15 HCMV wild strains. All of these were hybridized with probe IE. When primer MIE were used, 93%(14/15) of the wild strains showed amplified bands by direct gel analysis. When the urine specimens were amplified by PCR with primer IE, amplified bands were seen in 73%(11/15) by direct gel analysis ; 87%(13/15) by hybridization method. When primer MIE were used, 87%(13/15) of the urine specimens showed the PCR products by direct gel analysis. Conclusion : Polymerase chain reaction with primer IE and MIE may be a specific and sensitive diagnostic method for rapid detection of HCMV wild strains in Korea.

淸末 新政期의 敎育改革과 財政問題 : 江蘇省의 事例를 中心으로 the Case of Jiang-su Province

金衡鍾 韓國外國語大學校 歷史文化硏究所 1998 역사문화연구 Vol.8 No.-

Characterization of Antihypertensive Angiotensin I-Converting Enzyme Inhibitor from Recombinant E. coli

Kim, Jae-Ho,Jeong, Seung-Chan,Lee, Dae-Hyoung,Lee, Jong-Soo 배재대학교 자연과학연구소 2005 自然科學論文集 Vol.16 No.1

안지오텐신(ACE) 저해제는 항고혈 효과를 갖고 있으므로 오랫동안 고혈압의 예방이나 치료에 이용되어 왔다. 본 연구는 재조합 대장균으로부터 새로운 ACE 저해제를 생산하고 정제하며 나아가 이들이 구조-기능 관P를 규명하기 위해 수행되었다. Saccharomyces cerevisiase의 ACE 저해 펩타이드 유전자를 함유하고 있는 재조합 pGEX-4T-3을 대장균 BL21(DE3)로 형질전환 시켰다. 재조합 pGEX-4T-3을 갖고 있는 대장균 BL21(DE3)로부터 생산된 Glutathione-s 전이효소 (GST) 융합 단백질을 얻어서 그중 ACE저해 펩타이드를 Sephadex G-25 컬럼 크로마토그래피로 정제하였다. 정제된 ACE 저해 펩타이드는 타이로신-아스파틱엑시드-그리신-글리신-발린-패닐알라린-아르기닌-발린-타이로신-트레오닌의 서열을 가진 새로운 decapeptide이었고 ACE에 대하여 경쟁적으로 저해하였다. The angiotensin I-converting enzyme (ACE) inhibitor has anti-hypertensive effects and has long been used as prevention or remedy of hypertension. This study were carried out to produce and purify a new ACE inhibitor from recombinant E. coli and further elucidate its structure-function relationship. Recombinant pGEX-4T-3 containing ACE inhibitory peptide gene of Saccharomyces cerevisiae was transformed into E. coli BL21(DE3). Glutathione-S transferase (GST) fusion protein from E. coli BL21(DE3) harboring the recombinant pGEX-4T-3 was obtained and the ACE inhibitory peptide was purified with Sephadex G-25 column chromatography. The purified ACE inhibitory peptide was a novel decapeptide with sequence Tyr-Asp-Gly-Gly-Val-Phe -Arg-Val-Tyr-Thr which shows very low similarity to the other ACE inhibitory peptide sequence. The purified ACE inhibitor competitively inhibited ACE.

고효율 충전탑에서 유해가스처리

김장호,김형호,김종수 동아대학교 환경문제연구소 2001 硏究報告 Vol.24 No.2

In control technology of green house gas CO₂ exhausted, CO₂recovery by chemical absorption technology is recommended. In application to a power station and industrial field with using the absorbent MEA and AMF for carbon dioxide removal. we used packing tower(D=0.l5m, H=1.05m) for experimental apparatus on the chemical absorbtion being used extensively as recovery technique of CO₂. The pressure drop of structured packing Mellapak N. 250Y and random packing 25mm Pall nng was 5.2, 8.5 [mm H₂O/m in the gas capacity factor [kg^(1/2) m^(-1/2) s^(-1)]. Metallic Ralu-Pack 250YC, however, structured packing. has recently being used in petrochemical production plant in general. The decision of tower height depends on the number of transfer unit(NTU) and HTU influence on liquid distribute. If liquid flow is not good material separation, it is bad and usually occurs in the foolding point. In the normal condition, new systematic understanding is given on metal Ralu-Pack 250 YC.