Telomerase Reverse Transcriptase Contains a BH3-Like Motif and Interacts with BCL-2 Family Members

Jin, Young,You, Long,Kim, Hye Jeong,Lee, Han-Woong Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.7

Upregulation of human telomerase reverse transcriptase (hTERT) expression is an important factor in the cellular survival and cancer. Although growing evidence suggests that hTERT inhibits cellular apoptosis by telomere-independent functions, the mechanisms involved are not fully understood. Here, we show that hTERT contains a BH3-like motif, a short peptide sequence found in BCL-2 family proteins, and interacts with anti-apoptotic BCL-2 family proteins MCL-1 and BCL-xL, suggesting a functional link between hTERT and the mitochondrial pathway of apoptosis. Additionally, we propose that hTERT can be categorized into the atypical BH3-only proteins that promote cellular survival, possibly due to the non-canonical interaction between hTERT and antiapoptotic proteins. Although the detailed mechanisms underlying the hTERT BH3-like motif functions and interactions between hTERT and BCL-2 family proteins have not been elucidated, this work proposes a possible connection between hTERT and BCL-2 family members and reconsiders the role of the BH3-like motif as an interaction motif.

중환자 가족이 인지하는 간호사 의사소통유형과 중환자 가족 만족도와의 관계

이진영 ( Lee¸ Jin-young ),김화순 ( Kim¸ Hwasoon ) 부산대학교 간호과학연구소 2021 글로벌 건강과 간호 Vol.11 No.2

Purpose: This study aimed to investigate the relationships between family members’ perception of nurses communication styles and family satisfaction. Methods: This study adopted a descriptive correlational design. Participants were 118 family members of intensive care unit (ICU) patients. The following data were collected using questionnaires: general characteristics of participant, nurses’ communication styles, and satisfaction of critically ill patients’ family members. Results: The mean±standard deviation scores for informative, affiliative, and authoritative communication styles were 4.33±0.65, 4.06±0.73, and 1.73±0.80, respectively. There were no significant differences as per critically ill patients’ characteristics. However, family members’ satisfaction differed significantly by age. Family satisfaction sub-dimension scores on satisfaction with decision-making (F=3.36, p=.021) and care (F=3.33, p=.022) were significantly higher among family members under 40 than those between 40 and 49 years. Correlation coefficients among major variables were statistically significant (r=-.25 to r=.81), particularly those of nurses’ informative and affiliative communication styles with family satisfaction were higher than moderate. Conclusion: Thus, these results indicate that nurses in ICU should communicate using informative and affiliative styles to improve the satisfaction among family members of critically ill patients. For this purpose, all medical staff, especially, nurses and physicians should be provided with appropriate communication styles practice and continuous education on interaction with family members of ICU patients.

대승불교에 수용된 아라한의 지혜[羅漢四智] 연구 - 소작이판(所作已辦)의 의미를 중심으로

조진영 ( Cho¸ Jin-young ),강대현 ( Kang¸ Dae-hyun ) 동아시아불교문화학회 2021 동아시아불교문화 Vol.- No.47

나한사지란 초기불교의 출가수행자가 최초로 아라한과를 증득했을 때 스스로 송출하는 사구의 게송을 말한다. 즉 아생이진, 범행이립, 소작이판, 불수후유이다. 본고에서는 이들 중 소작이판의 의미가 대승경전에 적용된 양상을 살펴보았다. 소작이판은 수행자가 마땅히 해야 하는 제반의 수행적 의미를 지니고 있다. 그런데 초기불교에서 소작이판은 아라한과를 증득하기 위한 수행자의 완성적인 의미를 지녔다면, 대승불교에서는 성불로 가는 과정에서 수행자가 반드시 행해야 할 과정적 의미를 지닌 개념이라고 할 수 있다. 이러한 소작이판은 대승경전에서 붓다의 명호로 쓰이거나, 대승보살보다 열위에 있는 아라한의 소작이판, 유식불교에서 전식득지 개념의 소작성판과 비교되는 소작이판, 그리고 성문의 삼매도로 나타내어 불수순행의 주된 역할을 하는 밀교경전의 소작이판과 진언실지를 완성하는 역할의 소작이판 등 그 의미는 다소 차이가 있음을 알 수 있었다. 결과적으로 근본교설 내지 불교 제반의 수행도를 모두 함의하고 있는 나한사지, 그리고 네 가지 지혜 중에서 가장 적용 범위가 넓은 소작이판은 종파를 막론한 수행자의 필수불가결 덕목이라고 할 수 있다. The Four Kinds of Arhat’s Wisdom refer to gāthā[the poetry or verse of the Buddhist scriptures] recited by the early Buddhist priest when he first acquired the state of full attainment of arhatship. That is to say, ‘understanding that one has been born is the realization of the truth of suffering[我生已盡]’, ‘understanding that the arhat has completed the necessary practices of purity[梵行已立]’, ‘understanding that task of the arhat have been well performed[所作已辦]’, ‘understanding of the arhat that there will be no further rebirths[不受後有]’. In this paper, we looked at the ‘acquired that which is to be done’ of The Four Kinds of Arhat’s Wisdom. The ‘acquired that which is to be done’ has various performance meanings that the renunciant practitioner should do. However, in early Buddhism, the ‘acquired that which is to be done’ had a complete meaning of the state of full attainment of arhatship, but in Mahayana Buddhism, it has a processive meaning that must be performed by the renunciant practitioner in the process of going to becoming a buddha. The meaning was somewhat different depending on the flow of Buddhist history, including the ‘acquired that which is to be done’ may be used as the epithet of Buddha in a scripture of the great vehicle, the ‘acquired that which is to be done’ of arhat, which is inferior to the bodhisattva-mahāsattva, the ‘acquired that which is to be done’ compared to the completion of activities of the concept of the the Yogācāra Buddhist, in addition, the ‘acquired that which is to be done’ of the scriptures of Esoteric Buddhism, which is represented by the way of samādhi at the śrāvaka, plays a major role in the act of not being obedient to activity consciousness, and the ‘acquired that which is to be done’ of the role of completing the siddhis of mantra. As a result, The Four Kinds of Arhat’s Wisdom, which implies both fundamental doctrines and all Buddhist practices, and the ‘acquired that which is to be done’, which has the broadest application among The Four Kinds of Arhat’s Wisdom, can be said to be an indispensable virtue of all renunciant practitioners of all schools.

『聊齋志異』의 상고소설연구 ― 여성상인고사를 중심으로

宋眞榮 ( Song¸ Jin-young ) 중국어문학회 2021 中國語文學誌 Vol.- No.76

In Chinese literary works before the Ming Dynasty, merchants appeared as supporting roles and did not have a large proportion, but after the middle of the Ming Dynasty, when the commercial economy developed, they became an important protagonist who led the narrative. The depiction of the merchant in the works is also mostly greedy and immoral before the Ming Dynasty, and in contrast to the good protagonist, it was the subject of judgment or reprimand. On the other hand, after the middle of the Ming Dynasty, there were many appearances of good merchants who were sincere and possessed of Righteousness (義) and Profit (利), or Confucian Merchants (儒商) who pursued the Confucian ideology and Scholar-bureaucrat culture. This trend is also reflected in Liaozhaizhiyi (聊齋志異), which represents Classical Chinese novel in Qing Dynasty. In addition, unlike few female merchants appear in previous literary works or briefly appear as a merchant’s family and then disappear, there are many female merchants who show their abilities as much as men in Liaozhaizhiyi. Among them, works such as Xiao Er, Huang Ying, Xi Liu, and Bai Qiu Lian can be regarded as Merchant Novels (商賈小說) in which female merchants are the main characters and have many commercial descriptions, and reflect merchant values and culture. In these works, the female merchants are the undisputed protagonists of the narrative, demonstrating their ability to lead not only household chores but also commercial activities. They fulfilled the roles of women demanded by traditional society, namely, virtuous wives who help their husbands, and mothers who strictly educate their children. However, they did not stop there and moved out of the family and became a competent merchant. They also helped the poor around them, and although they were merchants, they showed the aspect of Confucian Merchants longed for scholars.

[PF-0032] Identification of the CaAN3 Gene that Regulates Fruit-specific Anthocyanin Biosynthesis in Pepper(Capsicum annuum)

Jinyoung Byun(Jinyoung Byun ),Tae-Gun Kim(Tae-Gun Kim ),Joung-Ho Lee(Joung-Ho Lee ),Ning Li(Ning Li ),Soyoung Jung(Soyoung Jung ),Byoung-Cheorl Kang(Byoung-Cheorl Kang ) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

Developmental competence of morphologically poor oocytes in relation to follicular size and oocyte diameter in the pig

Kim, Jinyoung,You, Jinyoung,Hyun, Sang-Hwan,Lee, Gene,Lim, Jeongmook,Lee, Eunsong Wiley Subscription Services, Inc., A Wiley Company 2010 Molecular reproduction and development Vol.77 No.4

<P>The objective of this study was to assess the practical usefulness of morphologically poor oocytes (MPCOCs) in relation to follicular size and oocyte diameter. Oocytes collected from medium (3–8 mm in diameter) and small (<3 mm) follicles were classified into five categories of morphologically good oocytes (MGCOCs) from medium follicles (MA, control), MPCOCs with larger and smaller diameters from medium follicles (ML and MS, respectively), and those from small follicles (SL and SS, respectively). The oocytes were examined for maturation and developmental competence after parthenogenesis and somatic cell nuclear transfer (SCNT). Nuclear maturation of ML oocytes (91%) was similar to that of control oocytes (94%), but higher than MS (80%), SL (79%), and SS (63%) oocytes. This pattern was also observed in the intracellular glutathione level, p34<SUP>cdc2</SUP> kinase activity, and gene (CDK1, PCNA, and ERK2) expression levels in in vitro-matured oocytes. ML oocytes showed a similar proportion of blastocyst formation (20%) after SCNT to control oocytes (21%). In addition, the use of ML oocytes resulted in a 50% farrowing rate with 1.8% efficiency of piglet production after SCNT embryo transfer, while control oocytes showed a 60% farrowing rate with 2.4% production efficiency. Our results demonstrate that MPCOCs, if appropriately selected, have a comparable ability to MGCOCs in supporting not only in vitro blastocyst formation, but also development to term in vivo after SCNT. These oocytes can be used as a source for in vitro production of embryos with normal in vivo viability in pigs. Mol. Reprod. Dev. 77: 330–339, 2010. © 2009 Wiley-Liss, Inc.</P>

MG132 treatment during oocyte maturation improves embryonic development after somatic cell nuclear transfer and alters oocyte and embryo transcript abundance in pigs

You, Jinyoung,Kim, Jinyoung,Lee, Heowwon,Hyun, Sang‐,Hwan,Hansen, Peter J.,Lee, Eunsong Wiley Subscription Services, Inc., A Wiley Company 2012 Molecular reproduction and development Vol.79 No.1

<P><B>Abstract</B></P><P>The objective of this study was to examine the effect of treating pig oocytes during in vitro maturation (IVM) with a proteasome inhibitor, MG132, on oocyte maturation and embryonic development. In one series of experiments, oocytes from medium‐sized follicles (3–8 mm in diameter) were untreated (MCO) or treated with MG132 during 0–22 hr (M0–22) or 30–42 hr (M30–42) of IVM. There was no significant effect of MG132 on nuclear maturation or cytoplasmic maturation (as assessed by intracellular amounts of glutathione and p34cdc2 kinase activity). Blastocyst formation after parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT), however, was increased for M30–42 (65.2% and 27.7% for PA and SCNT, respectively) compared to MCO (42.6% and 13.6%, respectively) and M0–22 (45.3% and 19.5%, respectively; <I>P</I> < 0.05). Expression of PCNA and ERK2 was increased in M30–42 for IVM oocytes while transcript abundance for POUF51, DNMT1, FGFR2, and PCNA was increased in M30–42 for 4‐cell SCNT embryos. When oocytes derived from small follicles (<3 mm in diameter) were untreated (SCO) or treated with MG132 during 0–22 hr (S0–22), 30–42 hr (S30–42) of IVM, or 0–22 and 30–42 hr of IVM (S0–22/30–42), expression of POU5F1, DNMT1, FGFR2, and PCNA and blastocyst formation were increased for SCNT embryos derived from S30 to 42 (16.5%) and S0–22/30–42 oocytes (20.8%) as compared to embryos from SCO (8.7%) or S0–22 oocytes (8.8%; <I>P</I> < 0.05). Results demonstrate that treatment of oocytes with MG132 during the later stage of IVM improves embryonic development and alters gene expression in pigs. Mol. Reprod. Dev. 79:41–50, 2012. © 2011 Wiley Periodicals, Inc.</P>

Post-fusion treatment with MG132 increases transcription factor expression in somatic cell nuclear transfer embryos in pigs

You, Jinyoung,Lee, Joohyeong,Kim, Jinyoung,Park, Junhong,Lee, Eunsong Wiley Subscription Services, Inc., A Wiley Company 2010 Molecular reproduction and development Vol.77 No.2

<P>The objective of this study was to examine the effect of post-fusion treatment of somatic cell nuclear transfer (SCNT) oocytes with the proteasomal inhibitor MG132 on maturation promoting factor (MPF) activity, nuclear remodeling, embryonic development, and gene expression of cloned pig embryos. Immediately after electrofusion, SCNT oocytes were treated with MG132 and/or caffeine for 2 hr, vanadate for 0.5 hr, or vanadate for 0.5 hr followed by MG132 for 1.5 hr. Of the MG132 concentrations tested (0–5 µM), the 1 µM concentration showed a higher rate of blastocyst formation (25.9%) than 0 (14.2%), 0.5 (16.9%), and 5 µM (16.9%). Post-fusion treatment with MG132, caffeine, and both MG132 and caffeine improved blastocyst formation (22.1%, 21.4%, and 24.4%, respectively), whereas vanadate treatment inhibited blastocyst formation (6.5%) compared to the control (11.1%). When examined 2 hr after fusion and 1 hr after activation, MPF activity remained at a higher (P < 0.05) level in SCNT oocytes that were treated post-fusion with caffeine and/or MG132, but it was decreased by vanadate. The rate of oocytes showing premature chromosome condensation was not altered by MG132 but was decreased by vanadate treatment. In addition, formation of single pronuclei was increased by MG132 compared to control and vanadate treatment. MG132-treated embryos showed increased expression of POU5F1, DPPA2, DPPA3, DPPA5, and NDP52l1 genes compared to control embryos. Our results demonstrate that post-fusion treatment of SCNT oocytes with MG132 prevents MPF degradation and increases expression of transcription factors in SCNT embryos, which are necessary for normal development of SCNT embryos. Mol. Reprod. Dev. 77: 149–157, 2010. © 2009 Wiley-Liss, Inc.</P>

Review on the Determination of Frumkin, Langmuir, and Temkin Adsorption Isotherms at Electrode/Solution Interfaces Using the Phase-Shift Method and Correlation Constants

( Jinyoung Chun ),( Jang H. Chun ) 한국화학공학회 2016 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.54 No.6

This review article described the electrochemical Frumkin, Langmuir, and Temkin adsorption isotherms of over-potentially deposited hydrogen (OPD H) and deuterium (OPD D) for the cathodic H₂ and D₂ evolution reactions (HER, DER) at Pt, Ir, Pt-Ir alloy, Pd, Au, and Re/normal (H₂O) and heavy water (D₂O) solution interfaces. The Frum-kin, Langmuir, and Temkin adsorption isotherms of intermediates (OPD H, OPD D, etc.) for sequential reactions (HER, DER, etc.) at electrode/solution interfaces are determined using the phase-shift method and correlation constants, which have been suggested and developed by Chun et al. The basic procedure of the phase-shift method, the Frumkin, Lang-muir, and Temkin adsorption isotherms of OPD H and OPD D and related electrode kinetic and thermodynamic param-eters, i.e., the fractional surface coverage (0 ≤ θ ≤ 1) vs. potential (E) behavior (θ vs. E), equilibrium constant (K), interaction parameter (g), standard Gibbs energy (ΔG_θ˚) of adsorption, and rate (r) of change of ΔG_θ˚ with θ (0 ≤ θ ≤ 1), at the interfaces are briefly interpreted and summarized. The phase-shift method and correlation constants are useful and effective techniques to determine the Frumkin, Langmuir, and Temkin adsorption isotherms and related electrode kinetic and thermodynamic parameters (θ vs. E, K, g, ΔG_θ˚, r) at electrode/solution interfaces.

Molecularly Imprinted Polymer-Based Electrochemical Sensors for the Detection of Trace Proteins

Jinyoung Park(박진영) 한국고분자학회 2023 한국고분자학회 학술대회 연구논문 초록집 Vol.48 No.1