Combined transcriptomic and proteomic analysis of flubendiamide resistance in Plutella xylostella

Li Jing‐Jing,Jin Ming‐Hui,Wang Nian‐Meng,Yu Qi‐Tong,Shang Ze‐Yu,Xue Chao‐Bin 한국곤충학회 2020 Entomological Research Vol.50 No.10

Diamondback moth (DBM), Plutella xylostella, is an important pest of crucifers worldwide. The extensive use of diamide insecticides has led to DBM resistance in the world, and this presents a serious threat to vegetable production. In the present study, transcriptomic and proteomic analyses were combined to investigate the potential flubendiamide‐resistance mechanism in DBM. The lab‐selected (Rh) and field‐collected (Rb) flubendiamide‐resistant lines of P. xylostella with resistance ratios of 1889.92‐fold and 1250.97‐fold, respectively, were used, as well as a lab‐reared flubendiamide‐susceptible line (S). Compared with the S group, the transcriptomic analysis revealed 151 upregulated and 287 downregulated gene messengers in the Rh group and 432 upregulated and 565 downregulated gene messengers in the Rb group. The most frequently enriched pathways of differentially expressed genes (DEGs) were mainly involved in metabolic pathways. Metabolism related genes, including two P450, two ABC transporters, and three trypsins, were upregulated in the Rh line. Additionally, some P450 genes, trypsin, juvenile hormone (JH), and mucin genes were also upregulated in the Rb line. In proteomic analysis comparisons with the S group, there were 78 upregulated and 90 downregulated proteins in the Rh group and 221 upregulated and 155 downregulated proteins in the Rb group. Further analyses found that three CYP and 11 CYP proteins were over‐expressed in Rh and Rb lines, respectively. Four glutathione S‐transferase (GST) and four UGTs were over‐expressed in Rb line. So, we deduced that the detoxification metabolism may be the main mechanism of flubendiamide resistance in P. xylostella.

Optical thermometry in low temperature through manipulating the energy transfer from WO₆ ⁶⁻ to Ho³⁺ in Y₂WO₆:Ho³⁺ phosphors

Wang, Jing,Bu, Yanyan,Wang, Xiangfu,Seo, Hyo Jin Elsevier 2018 Optical materials Vol.84 No.-

<P><B>Abstract</B></P> <P>Optical thermometry based on the adjacent thermally coupled levels of rare earth ions has been widely studied in the high temperature range, and shows unobvious effect at low temperature range. In this work, we propose a new method to achieve optical thermometry in 10˜300 K range through manipulating the energy transfer from WO<SUB>6</SUB> <SUP>6−</SUP> to Ho<SUP>3+</SUP>. A comprehensive five-level rate-equation model is developed to establish the relation between lifetime ratios and temperature. It is found that the lifetime ratios of 540 nm (Ho<SUP>3+</SUP>) and 471 nm (WO<SUB>6</SUB> <SUP>6−</SUP>) emissions are dependent on the phonon energy of Y<SUB>2</SUB>WO<SUB>6</SUB> host and temperature. The values of sensor sensitivity reach the maximum 2.1% K<SUP>−1</SUP> at 211 K in 30%Ho<SUP>3+</SUP> doped Y<SUB>2</SUB>WO<SUB>6</SUB>, which is higher than the maximum of the reported phosphors at low temperature. It overcomes the shortcoming of unobvious thermal population of thermally coupled levels in low temperature range through traditional Boltzmann distributing method.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Energy transfer process between host and Ho<SUP>3+</SUP> was analyzed. </LI> <LI> Temperature dependent emission spectra of Ho<SUP>3+</SUP> doped Y<SUB>2</SUB>WO<SUB>6</SUB> were studied. </LI> <LI> A new method to achieve optical thermometry through manipulating the energy transfer from WO<SUB>6</SUB> <SUP>6−</SUP> to Ho<SUP>3+</SUP> is proposed. </LI> </UL> </P>

The antimicrobial activity of as-prepared silver-loaded phosphate glasses and zirconium phosphate

Wang Jing,Ji Zhi Jiang,Shui Zhong He,Yang Yang,Zhao Chun Yan,Wang Xiao Yan 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.3

The antimicrobial activities of silver-loaded zirconium phosphate (JDG) and silver-loaded phosphate glasses (ZZB) against Escherichia coli were studied. Although the silver content in JDG was higher than that in ZZB, ZZB suspensions showed better antimicrobial property than JDG suspensions, especially at low concentrations. The antimicrobial activity was analyzed using minimum inhibitory concentrations, bacterial inhibition ring tests, and detection of silver ions in the suspensions. Furthermore, the amounts of silver ions in suspensions with/without bacterial cells were analyzed. Results revealed that only a portion of released silver ions could be adsorbed by E. coli cells, which are critical to cell death. The damaged microstructures of E. coli cells observed by transmission electron microscopy may further prove that the adsorbed silver ions play an important role in the antimicrobial process.

Long-term Care Nurses’ Communication Difficulties with People Living with Dementia in Taiwan

Jing-Jy Wang,Pei-Fang Hsieh,Chi-Jane Wang 한국간호과학회 2013 Asian Nursing Research Vol.7 No.3

Purpose: Impairments in word finding, language skills and memory in dementia patients increase the obstacles for health professionals to provide effective care. Although some research on communication with dementia patients has been done, no research that pre-assessed nurses’ difficulties in communicating with dementia patients has been identified. This study aims to explore nurses’ difficulties in communicating with patients who have dementia. Methods: This was a qualitative research using the phenomenological approach. Data were collected through in-depth interviews. Fifteen nurses with at least 6 months of dementia care experience participated in this study. Each interview was audio-taped and transcribed within 48 hours after each interview. Participants were asked to respond to the question, “Please describe the difficulties in communicating with patients who have dementia.”Results: Through content analysis, two themes, each with two subthemes emerged: Different language, including repetitive responses and lack of language consensus; blocked messages, including difficulty in accessing emotions and in understanding needs. Ineffective language refers to a lack of agreement dialect between the nurse and the patient while blocked messages refer to the inability of nurses to understand the true underlying meaning of messages the patients send out through verbal or nonverbal behaviors or expression. Conclusion: The results can serve as reference for planning dementia communication education for school curriculum to enhance student nurses’ communication abilities and for junior nurses working in long-term or acute care settings to increase nurses’ patient-centered communication abilities with the ultimate goal of improving quality of care for patients with dementia.

fects of Dietary Soy Intake on Maternal Thyroid Functions and Serum Anti-Thyroperoxidase Antibody Level During Early Pregnancy

Jing Li,Xiaochun Teng,Weiwei Wang,Yanyan Chen,Xiaohui Yu,Shen Wang,Jianxin Li,Lin Zhu,Chenyan Li,Chenling Fan,Hong Wang,Hongmei Zhang,Weiping Teng,Zhongyan Shan 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.5

Soy and its isoflavones have been suggested to suppress thyroperoxidase (TPO), induce goiter, inhibit deiodinase, and modulate immune functions. This study initially investigated the effects of dietary soy consumption on maternal thyroid functions and anti-TPO antibody (TPOAb) production during early pregnancy. Data were collected through questionnaire from 505 women enrolled during early pregnancy by random sampling in Shenyang, China. Based on soy intake frequency, the subjects were divided into three groups (frequent [three or more times per week], conventional [more than twice per month but less than three times per week], and occasional [two or fewer times per month]). Serum thyrotropin (TSH), free thyroxine (FT_4), and TPOAb were measured by chemiluminescence immunoassay. Additionally, the concentrations of two primary isoflavones (daidzein and genistein) and creatinine were assessed in the spot urine samples from representative subjects (about 20%) randomly selected from the three groups. The percentages of frequent, conventional, and occasional consumers were 18.6%, 62.6%, and 18.8%, respectively. No difference was found in age, medical records, family history of thyroid diseases, serum FT_4, TSH, and TPOAb levels, TPOAb-positive percentages, or prevalence of thyroid dysfunctions among the groups. Both urinary daidzein and genistein levels were significantly higher in the frequent consumers compared with the other two groups. No correlations were found between urinary isoflavone levels and serum FT_4 or TSH. Urinary isoflavone levels were not significantly different between TPOAb-positive and -negative women among the randomly selected representative subjects. On the whole, our findings suggest dietary soy consumption during early pregnancy is not associated with the development of thyroid dysfunction or autoimmunity.

On-line Auxiliary Input Signal Design for Active Fault Detection and Isolation Based on Set-membership and Moving Window Techniques

Jing Wang,Junde Wang,Meng Zhou 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.11

This paper presents an on-line auxiliary input signal design strategy based on set-membership and moving window techniques to deal with the problem of active fault detection and isolation. The goal of active fault detection and isolation is to design an auxiliary input signal, such that the nominal system output set and faulty systems output sets are separated each other after injecting the input signal. In this paper, the output sets are characterized by ellipsoids. First, an extended model of the system based on moving window technique is constructed, then an auxiliary input signal is calculated on-line based on the equivalent model. As the energy of the auxiliary input signal is restricted minimum to decrease the influence of the signal on the system, the design condition of active fault detection and isolation is transformed into an optimal problem. Furthermore, the fault is isolated by judging the actual system output belongs to which output ellipsoid of the faulty models, or determining the probability of the system output is in which faulty model when the output ellipsoids of faulty models are intersecting. Finally, numerical simulations illustrate the feasibility and effectiveness of the proposed approach.

Characterization of Brain Microstructural Abnormalities in High Myopia Patients: A Preliminary Diffusion Kurtosis Imaging Study

Wang Huihui,Wen Hongwei,Li Jing,Chen Qian,Li Shanshan,Wang Yanling,Wang Zhenchang 대한영상의학회 2021 Korean Journal of Radiology Vol.22 No.7

Objective: To evaluate microstructural damage in high myopia (HM) patients using 3T diffusion kurtosis imaging (DKI). Materials and Methods: This prospective study included 30 HM patients and 33 age- and sex-matched healthy controls (HCs) with DKI. Kurtosis parameters including kurtosis fractional anisotropy (FA), mean kurtosis (MK), axial kurtosis (AK), and radial kurtosis (RK) as well as diffusion metrics including FA, mean diffusivity, axial diffusivity (AD), and radial diffusivity derived from DKI were obtained. Group differences in these metrics were compared using tract-based spatial statistics. Partial correlation analysis was used to evaluate correlations between microstructural changes and disease duration. Results: Compared to HCs, HM patients showed significantly reduced AK, RK, MK, and FA and significantly increased AD, predominately in the bilateral corticospinal tract, right inferior longitudinal fasciculus, superior longitudinal fasciculus, inferior fronto-occipital fasciculus, and left thalamus (all p < 0.05, threshold-free cluster enhancement corrected). In addition, DKI-derived kurtosis parameters (AK, RK, and MK) had negative correlations (r = -0.448 to -0.376, all p < 0.05) and diffusion parameter (AD) had positive correlations (r = 0.372 to 0.409, all p < 0.05) with disease duration. Conclusion: HM patients showed microstructural alterations in the brain regions responsible for motor conduction and visionrelated functions. DKI is useful for detecting white matter abnormalities in HM patients, which might be helpful for exploring and monitoring the pathogenesis of the disease.

Minimum Bandwidth Regenerating Codes Based on Cyclic VFR Codes

( Jing Wang ),( Shuxia Wang ),( Tiantian Wang ),( Xuefei Zhang ) 한국인터넷정보학회 2019 KSII Transactions on Internet and Information Syst Vol.13 No.7

In order to improve the reliability and repair efficiency of distributed storage systems, minimum bandwidth regenerating (MBR) codes based on cyclic variable fractional repetition (VFR) codes are constructed in this thesis, which can repair failed nodes accurately. Specifically, in order to consider the imbalance of data accessed by the users, cyclic VFR codes are constructed according to that data with different heat degrees are copied in different repetition degrees. Moreover, we divide the storage nodes into groups, and construct MBR codes based on cyclic VFR codes to improve the file download speed. Performance analysis and simulation results show that, the repair locality of a single node failure is always 2 when MBR codes based on cyclic VFR codes are adopted in distributed storage systems, which is obviously superior to the traditional MBR codes. Compared with RS codes and simple regenerating codes, the proposed MBR codes based on cyclic VFR codes have lower repair locality, repair complexity and bandwidth overhead, as well as higher repair efficiency. Moreover, relative to FR codes, the MBR codes based on cyclic VFR codes can be applicable to more storage systems.

Relationship between EGFR Over-expression and Clinicopathologic Characteristics in Squamous Cell Carcinoma of the Esophagus: A Meta-analysis

Wang, Jun,Yu, Jin-Ming,Jing, Shao-Wu,Guo, Yin,Wu, Ya-Jing,Li, Na,Jiao, Wen-Peng,Wang, Li,Zhang, Yan-Jun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Over-expression of epidermal growth factor receptor (EGFR) has been identified as a common feature associated with clinical outcome in many types of cancer, including squamous cell carcinoma of the oesophagus (SCCO). However, the clinical importance of EGFR over-expression in SCCO remains unsettled as conflicting results exist. Therefore we carried out the present meta-analysis of published studies for clarification. A total of 13 studies including 1, 150 patients were enrolled. EGFR over-expression was positive in 722 of these cases. With EGFR over-expression, patients had higher depth of invasion, vascular invasion, and poor prognosis. However, expression had no relation with degree of differentiation, histological grade, lymph node metastasis, clinical stage or lymphatic invasion. EGFR over-expression is probably a valuable predictor for the T stage, vascular invasion and OS, and it could be used as a poor prognosis indicator for the esophageal SCC patients. Targeting therapy to EFGR should be considered to the combined treatment in SCCO.

LETTER TO THE EDITOR : One Mutation of the ED1 Gene in a Chinese Han Family with X-Linked Hypohidrotic Ectodermal Dysplasia

( Jing Wang ),( Wei Wei Ha ),( Wen Wang ),( Hua Yang Tang ),( Xian Fa Tang ),( Xian Dong Zheng ),( Jun Zhu ),( Xian Yong Yin ),( Sen Yang ),( Xue Jun Zhang ) 대한피부과학회 2014 Annals of Dermatology Vol.26 No.1