Pannonibacter tanglangensis sp. nov., a New Species Isolated from Pond Sediment

Wang Lei,Cheng Yanpeng,Yang Panpan,Zhang Jinjin,Zhang Gui,Zhang Sihui,Yang Jing,Zhang Zhen,Hu Lulu,Pu Ji,Yang Yanying,Lai Xin-He,Xu Jianguo,Li Yinghui,Hu Qinghua 한국미생물학회 2024 The journal of microbiology Vol.62 No.9

Two bacterial strains (XCT-34T and XCT-53) isolated from sediment samples of an artificial freshwater reservoir were analyzed using a polyphasic approach. The two isolates are aerobic, Gram-stain-negative, oxidase-negative, catalase-positive, motile with polar flagella, rod-shaped, and approximately 1.4–3.4 × 0.4–0.9 μm in size. Phylogenetic analyses based on 16S rRNA gene and whole-genome sequences showed that the two strains formed a distinct branch within the evolutionary radiation of the genus Pannonibacter, closest to Pannonibacter carbonis Q4.6T (KCTC 52466). Furthermore, lower than threshold average nucleotide identity values (ANI, 85.7–86.4%) and digital DNA–DNA hybridization values (dDDH, 22.3–30.5%) of the two strains compared to the nearest type strains also confirmed that they represented a novel species. Genomic analyses, including annotation of the KEGG pathways, prediction of the secondary metabolism biosynthetic gene clusters and PHI phenotypes, supported functional inference and differentiation of the strains from the closely related taxa. Results of chemotaxonomic and physiological studies revealed that their distinct phenotypic characteristics distinguished them from existing Pannonibacter species. Thus, the two strains are considered to represent a novel species of Pannonibacter, for which the name of Pannonibacter tanglangensis sp. nov. is proposed, with XCT-34T (= KCTC 82332T = GDMCC 1.1947T) as the respective type strain.

Structural Characterization of Microwave-Assisted Solution-Synthesized Strontium-Substituted Hydroxyapatite

Jianguo Liao,Yanqun Li,Xiali Guan,Jingxian Liu,Yongxiang Zhang,Yufen Xie,Zhengpeng Yang,Xingze Duan,Aiguo Zhou,Jiangnan Zhu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.10

Hydroxyapatite and strontium-substituted hydroxyapatite nanoparticles (n-HA and Sr-HA) were prepared by microwave-assisted solution synthesis with aqueous solutions of various Sr/(Sr+Ca) molar ratios ranging from 0% to 15%. The structural properties of the hydroxyapatite powders were investigated by X-ray powder diffraction, Fourier transform infrared spectroscopy and Raman spectra, field emission scanning electron microscopy coupled with energy dispersive X-ray spectrometry and transmission electron microscopy. The results confirmed that strontium ions had been incorporated into the hydroxyapatite lattice. The synthetic n-HA and Sr-HA nanocrystalline consisted of hydroxyapatite crystalline phase with hexagonal structure, and the particle size was 30–40 x 60–70 nm and 40–50 x 70–80 nm, respectively. The calcined HA particle size ranged from about 120 nm to 150 nm, the calcined Sr-HA products were composed of spherical aggregates with a size of about 70–100 nm. The incorporation of Sr ions lead to the formation of vacancies in the crystal structure of the HA. The results indicated that the strontium substitution did not change the crystal structures. More Sr resulted in less calcined crystallites and formed agglomerates owing to the size effect.

Morphological characteristics and gene mapping of a dense panicle (dp2) mutant in rice (Oryza sativa L.)

Yunlong Yang,Chunhai Shi,Yuanfei Zhou,Jianguo Wu,Xiaoli Jin,Jianyao Shou 한국유전학회 2014 Genes & Genomics Vol.36 No.3

A dense panicle mutant (dp2) derived from theOryza sativa ssp. japonica cultivar Nipponbare through ethylmethane sulfonate mutagenesis was used in present study. Compared to the wild type, the panicle of dp2 mutantexhibited more branches and denser grains. Further more, thenumber of spikelets per panicle, number of primary branchesand secondary branches of dp2 mutant were significantlyincreased while the panicle length, and 1,000-grain weightwere significantly decreased. The results from the geneticanalysis indicated that the dense panicle phenotype wascontrolled by a single dominance nuclear gene. Polymorphicanalysis of SSR and InDel markers demonstrated that theDP2 gene was located at the long arm of chromosome 2,which was further mapped between SSR markers RM341and RM13356 in a physical region of 398 kb. Within thisregion, the RCN2 (LOC_Os02g32950) gene which wasannotated relating to the development of rice panicle wasfound. Compared to the wild type, the sequence of RCN2gene in the dp2 mutant showed that two SNPs replacementhad taken place in the promoter region (G–A) and the intronregion (A–T), respectively. The dp2 mutant could be a novelmutant of RCN2 gene and this novel mutant might be usefulfor further studies on this gene.

Nanoclay, Intumescent Flame Retardants, and Their Combination with Chemical Modification for the Improvement of the Flame Retardant Properties of Polymer Nanocomposites

Guobo Huang,Jianguo Yang,Xu Wang,Jianrong Gao 한국고분자학회 2013 Macromolecular Research Vol.21 No.1

The flame retardancy of nanoclay, intumescent flame retardants (IFRs), and their combination with chemical modification is investigated in poly(methyl methacrylate) (PMMA) nanocomposites. 2,4,8,10-Tetraoxa-3,9-diphosphaspiro[5.5]-undecane-3,9-dioxide-disubstitutio-acetamide-N,N,N-triethyl-ammonium bromide (PDEABMMT)and 2,4,8,10-tetraoxa-3,9-diphosphaspiro[5.5]-undecane-3,9-dioxide-disubstitutio-acetamide-N,N-dimethyl-N-hexadecyl-ammonium bromide (PDHAB-MMT) are prepared by ion exchange of sodium montmorillonite with phosphorus-nitrogen containing quarternary ammonium salts including PDEAB and PDHAB. The X-ray diffraction (XRD) and transmission electron microscopy (TEM) results show that PDHAB-MMT achieves tood dispersion in the PMMA matrix and the corresponding nanocomposites PMMA/PDHAB-MMT are formed. The incorporation of PDHAB-MMT into the PMMA matrix obviously improves the nanocomposite thermal properties. Cone calorimeter experiments indicate that the flame retardancy of nanoclay, IFRs, and their combination with chemical modification showed a 5%, 5%, and 9% reduction in the peak heat release rates of PMMA, respectively. The scanning electron microscopy and TEM images confirm that a compact and dense char is formed for PMMA/PDHAB-MMT nanocomposites after combustion.

Novel Ceramides from Aerial Parts of Saussurea involucrata Kar. et. Kir.

Wei Wu,Yang Qu,Huiyuan Gao,Jingyu Yang,Jianguo Xu,Lijun Wu 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.9

Novel ceramides, together with nine known compounds were isolated from aerial parts of the Saussurea involucrata Kar. et. Kir. The novel structures were determined by spectroscopic methods and microscale chemical degradation. The ceramides showed appreciable cytotoxicity against three human tumor cell lines including human promyelocytic leukemia (HL-60), human melanoma (A375-S2) and human cervical carcinoma (HeLa) cell lines. The results suggested that this class of ceramides may have potential as an antitumor agent.

Effect of Surface Waviness on the Performances of an Aerostatic Thrust Bearing with Orifice-Type Restrictor

Hui Zhuang,Jianguo Ding,Peng Chen,Yu Chang,Xiaoyun Zeng,Hong Yang,Xingbao Liu,Wei Wei 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.22 No.10

In this paper, four waviness types are presented to investigate the effect of surface waviness on the static performance, dynamic performance and stability of an aerostatic thrust bearing with orifice-type restrictor. The influences of wave amplitude and rotational speed on the bearing performances are studied. The Reynolds equation is first solved through small perturbation method to obtain the static and dynamic characteristics. Subsequently, by adopting an iterative method based on the acquired dynamic coefficients, a numerical method is proposed to analyze the bearing stability. The calculated critical supply pressure is compared with that obtained by the traditional orbit method and it indicates that the proposed method has higher computational efficiency. The results show that the surface waviness has great influence on the bearing performances and the effect becomes increasingly pronounced with the growth of wave amplitude. The performances are more sensitive to the waviness arranged in bearing radial direction than circumferential direction. The enhancement of certain performance is always accompanied by the decline of another performance for the bearing with wave pattern on the surface, which leads to the fact that the bearing performances cannot be comprehensively improved in static characteristic, dynamic characteristic and stability. Besides, high rotational speed will cause pneumatic instability more readily. Finally, a stiff ness measuring experiment and a modal test are performed to verify the accuracy of computational analysis and results.

Outcomes of Posterior Lumbar Hemivertebra Resection and Short Fusion in Patients With Severe Sacral Tilt

Shengru Wang,Jianguo Zhang,Yanbin Zhang,You Du,Yang Yang,Guanfeng Lin 대한척추신경외과학회 2021 Neurospine Vol.18 No.3

Objective: To introduce surgical strategies to restore coronal balance during limited fusion for early lumbar hemivertebra resection in patients with severe sacral tilt. Methods: Sacral tilt was defined as a sacral tilt angle ≥5, and severe sacral tilt was defined as a sacral tilt angle >10. From July 2004 to December 2017, 73 consecutive patients treated with posterior hemivertebra resection and short fusion in our institution were evaluated. Severe sacral tilt was noted in 26 patients (14 boys and 12 girls), and all were enrolled in this study. Undercorrection of the primary lumbar curve as compensation for the sacral tilt and short fusion was performed in these patients. The medical charts and imaging data of the patients were retrospectively reviewed to evaluate the outcomes. Results: All patients were followed for at least 2 years. The mean age at the time of surgery was 3.7 (2–9) years old, with a total of 31 lumbar hemivertebra excised. On average, 2.8 (2–5) segments were fused for each patient. Sacral tilt minimally improved from 14.5° preoperatively to 13.6° postoperatively (p=0.15) and remained stable at the follow-up. The overall lumbar curve was 41.9° preoperatively, 11.7° immediately postoperatively, and 14.6° at the final follow-up. The segmental scoliosis curve was 39.1° preoperatively, 9.7° immediately postoperatively, and 11.2° at the final follow-up. Segmental kyphosis was corrected from 27.2° to 6.5° after the surgery and was 7.1° at the latest follow-up. Conclusion: Sacral tilt is seen in patients with congenital scoliosis in lumbar hemivertebra. Undercorrection of the lumbar curve and segmental scoliosis to compensate for sacral tilt and short fusion after hemivertebra resection may be helpful to restore coronal balance and preserve mobility in segments in patients with pronounced severe sacral tilt.

Changpingibacter yushuensis gen. nov., sp. nov., isolated from fluvial sediment in Qinghai Tibet Plateau of China

Jiao Yifan,Zhang Sihui,Yang Jing,Lai Xin-He,Dong Kui,Cheng Yanpeng,Xu Mingchao,Zhu Wentao,Lu Shan,Jin Dong,Pu Ji,Huang Ying,Liu Liyun,Wang Suping,Xu Jianguo 한국미생물학회 2022 The journal of microbiology Vol.60 No.2

Two facultatively anaerobic, short rod-shaped, non-motile, Gram-stain-positive, unknown bacterial strains (JY-X040T and JY-X174) were isolated from fluvial sediments of Tongtian River in Yushu Tibetan Autonomous Prefecture, Qinghai province, China. Cells formed translucent, gray, round and convex colonies, with a diameter of less than 0.5 mm after 5 days of incubation at 30°C on brain heart infusion-5% sheep blood agar. The 16S rRNA gene sequence similarity between strain JY-X040T and Fudania jinshanensis 313T is 93.87%. In the four phylogenetic trees constructed based on the 16S rRNA gene and 423 core genes, the two isolates form an independent branch, phylogenetically closest to F. jinshanensis 313T, but could not be classified as a member of the genus Fudania or any other genus of the family Arcanobacteriaceae. The DNA G + C content of strain JY-X040T was 57.8%. Calculation results of average nucleotide identity, digital DNADNA hybridization value and amino acid identity between strain JY-X040T and F. jinshanensis 313T are 69.9%, 22.9%, and 64.1%. The major cellular fatty acids were C16:0 (23%) and C18:1ω9c (22%). The cell-wall peptidoglycan type was A5α (L-Lys-L-Ala-L-Lys-D-Glu). The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and four unidentified components. The whole-cell sugars contained rhamnose and ribose. MK-10(H4) was the sole respiratory quinone. The minimum inhibitory concentration of streptomycin was 32 μg/ml. All physiological, biochemical, chemotaxonomic and genomic characteristics support that strains JY-X040T and JY-X174 represent members of a novel species in a new genus, Changpingibacter yushuensis gen. nov., sp. nov. The type strain is JY-X040T (GDMCC 1.1996T = KCTC 49514T).

Effect of Land-use Types on Soil Organic Carbon and Particulate Organic Carbon in Ecotones of Agriculture and Animal Husbandry

Bo Deng,Jinge An,Jianguo Han,Fuyu Yang,Yingjun Zhang 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08

In this paper, the effect of different land-use types on soil organic carbon (SOC) and Particulate Organic Carbon(POC) was studied by analyzing the soils in the meadow and conversing land of grassland to cropland in ecotones of agriculture and animal husbandry. It is shown t㏊t, compared with meadow, rotations of P/O and P/Cwill reduce SOC in 0-30㎝ soil layers by 47.54%-65.11% and 34.16-64.26% and POC by 16.90%-24.48% and 11.94%-20.29%, respectively. And SOC in meadow is more t㏊n in conversing land of grassland to cropland (P<0.05). Conversing grassland to cropland in ecotones of agriculture and animal husbandry will decrease the contents of SOC and POC.

Temporal dynamics of Bactrocera (Zeugodacus) tau (Diptera: Tephritidae) adults in north Jiangxi, a subtropical area of China revealed by eight years of trapping with cuelure

Xiaozhen Li,Haiyan Yang,Kaiping Hu,Jianguo Wang 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.1

Bactrocera (Zeugodacus) tau (Walker) (Diptera: Tephritidae), a quarantine pest of international concern, has become widespread and economically important in South and Southeast Asia. It has been introduced into north Jiangxi from Fujian, China and become a threat to local fruits and vegetables. Field trapping with cuelure (CUE) as lure is needed, not only for monitoring population dynamics of B. tau but also for direct control of population. In this study, we collected weekly tephritids from 4 CUE-baited McPhail traps every year from 2008 to 2015, analysing seasonal and inter-year B. tau population fluctuations. The total number of captured tephritids reached 7811, including B. tau (5321), B. scutellata (Hendel) (2375) and B. cucurbitae (Coquillett) (1 1 5). Among them, B. tau was the dominant species. It had 3–4 generations a year in north Jiangxi with high generation overlapping. The average number of trapped B. tau males did not differ significantly in different years, while the average monthly number showed large variation from June to December. No B. tau males were trapped before June in any year. Adult males were present from mid-June to late December, with distinct peaks between late August and mid-October. This fruit fly can withstand below 13℃ as a monthly minimal temperature under field conditions, and adult capture rates were significantly related to monthly minimal temperature and monthly total precipitation. These results can be used to predict the occurrence time and population size of B. tau in different seasons, enabling growers and extension personnel to take efficient management measures before they damage the host crops.