A New Triterpenoid from Panax ginseng Exhibits Cytotoxicity through p53 and the Caspase Signaling Pathway in the HepG2 Cell Line

Jian Huang,Xiao-hui Tang,Takashi Ikejima,Xiu-jia Sun,Xiao-bo Wang,Rong-gang X,Li-jun Wu 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.3

A new triterpenoid, 20(R),22( ξ),24(S)-dammar-25(26)-ene-3 β,6 α,12 β,20,22,24-hexanol (1), and three known triterpenoids, β-D-glucopyranoside,(3 β,12 β)-12,20-dihydroxydammar-24-en- 3-yl,6-acetate (2), 20(R)-ginsenoside Rg3 (3), and 20(R)-ginsenoside Rh2 (4), were isolated from the leaves of Panax ginseng. Their structures were determined by chemical analysis and spectral methods (IR, 1D and 2D NMR, HR-ESI-MS). Compounds 1-4 were exhibited various degrees of cytotoxicity in the human hepatoma cell line, HepG2. Compound 1 had the highest cytotoxic potency, with an IC50 value of 20.1 µM, by stimulating p53-mediated cell cycle arrest at the G1 to S phase transition, leading to apoptosis via activation of the caspase signaling pathway.

Preparation and Properties of Modified Silicon-containing Arylacetylene Resin with Bispropargyl Ether

Jian Zhang,Jianxiang Huang,Xiaojiao Yu,Canfeng Wang,Farong Huang,Lei Du 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.11

A novel silicon-containing arylacetylene resin (MSAR) modified by dipropargyl ether of bisphenol A (DPBPA) and dipropargyl ether of perfluorobisphenol A (DPPFBPA) was prepared separately. The curing behaviors of modified resins, DPBPA/MSAR and DPPFBPA/MSAR, were characterized with differential scanning calorimeter (DSC). The kinetic parameters of modified resins were obtained by the Kissinger and Ozawa methods. The results of dynamic mechanical analysis (DMA) revealed that the glass transition temperature (Tg) of the cured DPBPA/MSAR reached 486 °C. According to the thermogravimetric analysis (TGA), the decomposition temperature (Td5) of the cured resins and char yield (Yc, 800 °C) decreased as the dipropargyl ether loadings increased, especially in air. With the same weight loading, thermal stability of DPBPA/MSAR was better than that of DPPFBPA/MSAR. The carbon fiber (T300) reinforced composites exhibited excellent flexural properties at room temperature with a high property retention at 300 °C.

Proton Conducting Behavior of a Novel Composite Based on Phosphosilicate/Poly(Vinyl Alcohol)

Huang, Sheng-Jian,Lee, Hoi-Kwan,Kang, Won-Ho The Korean Ceramic Society 2005 한국세라믹학회지 Vol.42 No.2

A series of proton conductive composite membranes based on poly(vinyl alcohol) and phosphosilicate gels powders were successfully prepared. The proton conductivity of these composite was attributed to the phosphosilicate gel, which derived from tetraethoxysilane and phosphoric acid by sol-gel process at a molar ratio of P/Si = 1.5. The proton conductivity increased with increasing both the content of phosphosilicate gel and relative humidity. Temperature dependence of conductivity showed a Vogel-Tamman-Fulcher type behavior, indicating that proton was transferred through a liquidlike phase formed in micropores of phosphosilicate gel. The high conductivity of 0.065 S/cm with a membrane containing 60 wt$\%$ of the gel was obtained at $60^{\circ}C$ at $90\%$ relative humidity.

The potential role of COX-2 in cancer stem cell-mediated canine mammary tumor initiation: an immunohistochemical study

Jian Huang,Di Zhang,Fuqiang Xie,Degui Lin 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.2

Increasing evidence suggests that cancer stem cells (CSCs) are responsible for tumor initiation and maintenance. Additionally, it is becoming apparent that cyclooxygenase (COX) signaling is associated with canine mammary tumor development. The goals of the present study were to investigate COX-2 expression patterns and their effect on CSC-mediated tumor initiation in primary canine mammary tissues and tumorsphere models using immunohistochemistry. Patterns of COX-2, CD44, octamer-binding transcription factor (Oct)-3/4, and epidermal growth factor receptor (EGFR) expression were examined in malignant mammary tumor (MMT) samples and analyzed in terms of clinicopathological characteristics. COX-2 and Oct-3/4 expression was higher in MMTs compared to other histological samples with heterogeneous patterns. In MMTs, COX-2 expression correlated with tumor malignancy features. Significant associations between COX-2, CD44, and EGFR were observed in low-differentiated MMTs. Comparative analysis showed that the levels of COX-2, CD44, and Oct-3/4 expression varied significantly among TSs of three histological grades. Enhanced COX-2 staining was consistently observed in TSs. Similar levels of staining intensity were found for CD44 and Oct-3/4, but EGFR expression was weak. Our findings indicate the potential role of COX-2 in CSC-mediated tumor initiation, and suggest that COX-2 inhibition may help treat canine mammary tumors by targeting CSCs.

Research on Gateway Deployment Solving Model Applied in the Wireless Mesh Network

HUANG Jian-Heng,YU Jun-Qi,ZHAO Min-Hua 보안공학연구지원센터 2015 International Journal of Future Generation Communi Vol.8 No.3

In order to solve gateway deployment of geometry K center in the wireless network and improve the service quality of network, this paper proposes the gateway deployment solving model and algorithm. This paper transforms center problem of the geometric K into center problem of node K and solves problem. This paper proposes a gateway node alternative selection algorithm combination of network topology properties and maximum cover area related theory, and according to the relevant features select an alternative area, each alternative area abstract for a virtual node and is inserted into the original network topology structure, thus forming the new network topology. Solving new problem of the network topology and looking for the optimal deployment scheme use the improved genetic algorithm. The experimental results show that the model and algorithm proposed in this paper can well solve the wireless network gateway deployment problem of geometric K center and improve the network service quality.

Social Network Partition Method based on Concentricity-Controllable Routing

Huang Jian-Heng,Yu Jun-Qi,Zhao Min-Hua 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.4

In allusion to issues that partitioning algorithm of traditional social network community is generally lacking in comprehensive consideration of node and link attributes as well as full expression of model and mechanism using attributes of node and link. This paper presents a social network community partitioning algorithm fusing node and link attributes. The algorithm has blended similarity of node properties, link weights between nodes and link attribute information, making definition of the similar weights, and on this basis, social networking community division is realized combined with condensation algorithms. Experiments show that the algorithm has remarkable effects on community partition with obvious attributes in social network.

Detection of Bearing/Rail Defects for Linear Motion Stage Using Acoustic Emission

Huang Jian,안중환,이효렬 한국정밀공학회 2013 International Journal of Precision Engineering and Vol. No.

This paper is about development of an AE signal-based monitoring system for detecting defects on the bearings and rails of an LMS which is widely used in the automated production lines. It is examined whether the defects really contribute to generation of the AE signal and what frequency components of the AE signal are closely related to the defects. The cepstrum analysis is adopted to get a time interval of the purely defect-driven AE wavelets, which is compared with the theoretical interval based on the kinematic modeling of LM rail and bearing of an LMS. From the defect-driven AE wavelet picked up from a series of AE signal, the frequency features very unique to each defect of Bearing/Rail are extracted. The features are grouped three bands, that is, 100-150, 150-200,400-450 kHz. Three-band power spectrum shows different patterns each other for normal, rail defect, small bearing defect and worse bearing defect. Through these experiments, it is verified that AE signal is useful to detect the bearing/rail defect and a couple of bands around the AE frequency features can be used as a real-time monitoring device for the status monitoring of LMS.

고고학과 인류학의 관계

Huang, Jian-Qiu 호남고고학회 2003 湖南考古學報 Vol.17 No.-

Archaeology is a science that seeks for reconstruction of prehistory, supplements and corrects historical documents, and explores process and laws of human history and culture, through examination of material remains. Process of archaeological examination in general starts from material remains(實物), followed by technology-function(事物) and humans(人物). It is archaeologists’ task to develop ways to reach understanding of humans from material remains through technology-function. I suggest that in order to accomplish this, we refer to anthropological methods and theories. This paper attempts to explain how to interpret the differences in shapes of artifacts by adopting anthropological method and theory, looking at decorations on handles of Jade-axes from Liangjhu culture. Examination of jade goods from Liangze culture suggests that because various production locales which have different norms of and strict controls over production and consumption competed, cultural elements diffused from one group to others were usually modified by recipients. From this example, it can be suggested that interpretation of archaeological culture process by adopting anthropological methods and theories aims at looking at humans through material remains.

Preparation and Characterization of Composite Membrane for Low Temperature Direct Methanol Fuel Cells

Huang Sheng-Jian,Lee Hoi-Kwan,Kang Won-Ho,Wu Qing The Korean Microelectronics and Packaging Society 2004 마이크로전자 및 패키징학회지 Vol.11 No.4

A series of $H_3PO_4$-doped composite membranes based on poly(vinyl alcohol)(PVA) and silica have been prepared by sol-gel process. The proton conductivity, as well as properties of swelling, methanol permeation, was measured in this study. The proton conductivity increased with the molar ratio of $H_3PO_4$ to silica. With the silica content increasing, swelling degree decreased and methanol permeability showed a slight increase. It suggested that the former was mainly determined by hydrophilicity of the membrane, while the latter was dominated by the interconnectivity of matrix. According to the value of on, the optimal conformations of these composite membranes were 60, 70, 80 wt.$\%$ of PS-x in membranes, where x were 1.5, 1.0, and 0.5, respectively. These composite membranes were thermal stability up to $200^{\circ}C$.

A New Triterpenoid from Panax ginseng Exhibits Cytotoxicity through p53 and the Caspase Signaling Pathway in the HepG2 Cell Line

Huang, Jian,Tang, Xiao-Hui,Ikejima, Takashi,Sun, Xiu-Jia,Wang, Xiao-Bo,Xi, Rong-Gang,Wu, Li-Jun 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.3

A new triterpenoid, 20(R),22$({\zeta})$,24(S)-dammar-25(26)-ene-$3{\beta},6{\alpha},12{\beta}$,20,22,24-hexanol (1), and three known triterpenoids, ${\beta}$-D-glucopyranoside,($3{\beta},12{\beta}$)-12,20-dihydroxydammar-24-en-3-yl,6-acetate (2), 20(R)-ginsenoside $Rg_3$ (3), and 20(R)-ginsenoside $Rg_2$ (4), were isolated from the leaves of Panax ginseng. Their structures were determined by chemical analysis and spectral methods (IR, 1D and 2D NMR, HR-ESI-MS). Compounds 1-4 were exhibited various degrees of cytotoxicity in the human hepatoma cell line, HepG2. Compound 1 had the highest cytotoxic Potency, with an $IC_{50}$ value of 20.1 ${\mu}M$, by stimulating p53-mediated cell cycle arrest at the G1 to S phase transition, leading to apoptosis via activation of the caspase signaling pathway.