3,4‑Dichloroaniline promotes fatty liver in zebrafish larvae

Ji‑Seon Park,송정아,Jong‑Su Park,이상우,Jieon Lee,Han‑Jin Park,Woo‑Keun Kim,Seokjoo Yoon,Hang‑Suk Chun 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.2

Backgrounds 3,4-Dichloroaniline (3,4-DCA) is a transformation product of herbicides that is commonly used as a reference in developmental toxicity studies (OECD TG 236) (Bonnet et al. in Environ Toxicol 22:78–91, 2007). However, the mechanisms underlying 3,4-DCA-induced hepatotoxicity are not well known. Methods We exposed zebrafish larvae at 72 hpf to 3,4-DCA for 3 days and observed lipid accumulation in liver treated with 10-μM 3,4-DCA using oil red O staining. Subsequently, we performed qRT-PCR analysis to determine the genes involved in the observed lipid accumulation. Results We found that genes related to lipogenesis (srebp1, pparγ, lipin1, and scd1) and ER stress (bip, atf4, ddit3, dnajc3, and edem1) were significantly upregulated. In addition, we found that ROS generation increased in the larvae treated with 10-μM 3,4-DCA. Moreover, glutathione-S-transferase activity in these larvae was increased by 3,4-DCA in a dose-dependent manner, and the expression of the inflammation marker il-1β increased. Conclusion Our results indicated that exposure to 3,4-DCA induced fatty liver in zebrafish larvae and that this, in association with additional factors such as ER stress response, can promote liver damage. We accordingly suggest that 3,4-DCA could be used to induce fatty liver in zebrafish larvae.

지역사회거주 노인에서 한국어판 세계보건기구 장애평가조사표의 개반

윤진상,김재민,신일선,양수진,정태길,이형영 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.1

지역사회거주 노인에서 한국어판 세계보건기구 장애평가조사표의 개발

윤진상,김재민,신일선,양수진,정태길,이형영 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.1

Objectives : This study aimed to develop the Korean version of World Health Organization Disability Assessment Schedule II (WHODAS II-K) with community dwelling elderly population. Methods : The WHODAS II-K was administered to 1204 community residents aged 65 or over in two areas of Kwangju, South Korea, in 2001. For assessing 'health condition', data on physical illness, depression (Korean version of Geriatric Mental State Schedule B3), and cognitive dysfunction (Korean version of Mini-Mental State Examination) were collected. For evaluating 'contextual factors', informations on demographic charactehstics (age, gender, living area, marital state, and religiou), socio-economic status (education, type of accommodation, number of room, previous occupation, and current employment), and social network were obtained. Results : WHODAS II-K showed high levels of internal consistency, split-half reliability, and inter-rater and test-retest reliabilities. In the correlation analyses, scores on the WHODAS II-K were significantly correlated with the unfavorable conditions in the all variables on health condition and contextual factors. Partial correlations of scores on the WHODAS II-K with health condition were significant even after controlling for contextual factors. Conclusion : The WHODAS II-K is a reliable and valid instrument for assessing disability in elderly population since it reflects physical illness, depression, and cognitive impairment, which are common in elderly.

도시 쉼터 노숙자의 정신장애 유병율과 삶의 질

한오수,홍진표,하지혜,이철,김창윤,이동우,박종익 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.5

연구목적 : 외환위기 이후에 급증한 노숙자 문제는 경제적인 상황의 호전에도 불구하고 만성화하는 경향을 보이고 있다. 쉼터의 노숙자를 대상으로 정신질환의 유병율과 삶의 질에 대한 조사를 시행하였다. 방 법 : 2000년 3월에 서울의 대표적인 쉼터에 거주하는 노숙자220명을 대상으로 인구학적 자료에 대한 설문조사를 한 뒤 Structured Clinical for DSM-Ⅳ(SCID-I)을 이용하여 AxisⅠ의 주요 정신질환을 진단하였다. 또 SmithKline Beecham Quality of Life Scale(SBQOL)를 사용하여 삶의 질정도를 평가하였다. 결 과 : DSM-Ⅳ AxisⅠ의 정신질환 중 기분장애, 정신병적 장애, 물질 사용 장애에 대한 진단 평가 결과 상기정신질환의 전체 평생 유병율은 73.6%, 현재 유병율은 59.1%이었다. 알코올 의존과 남용의 평생 유병율은 59.5%로 가장 높았고, 기분장애와 장애가 각각 39.5%, 3.6%이었다. 삶의 질은 노숙의 기간이나 실직 기간과 유의한 관계가 없었고, 자신이 지각한 건강상태, 자살과거력, 우울장애 유무와 통계적으로 유의하게 상관관계가 있었다(p<0.01). 결 론 : 노숙자에서 정신질환 중 알코올 장애의 유병율이 가장 높았으며, 우울장애가 있는 경우에 삶의 질이 저하되는 것으로 나타났다. Objectives : Homeless people surged after financial crisis tend to be chronic despite late economic recovery. So we tried to estimate prevalence of mental illness and quality of life of the homeless population living in shelter. Method : The study subjects were 220 homeless peoples who stayed at a shelter in Seoul around March 2000. Questionnaires on sociodemographic data were administered to the subjects, and then diagnoses of major DSM-Ⅳ Axis I mental disorders were made using Structured Clinical Interview for DSM-Ⅳ Axis I Disorders(SCID). And quality of life(QOL) was evaluated by SmithKline Beecham Quality of Life(SBQOL). Results : The lifetime prevalence of major DSM-Ⅳ mental disorders(mood disorders, psychotic disorder, and substance use disorder) of 220 homeless people was 73.6%, and current prevalence was 59.1%. The lifetime prevalence of alcohol dependence and abuse was highest, 59.5% and those of mood disorder and psychotic disorder were 39.5%, 3.6% respectively. Quality of life was not significantly correlated with duration of homelessness and unemploy-ment but marital status, self perceive health status, suicidal attempt history were significant factors. The quality of life in the people with mood disorders were estimated to be lower than those with any other diseases(p<0.01). Conclusion : Alcoholism was the most prevalent mental disorder in homeless people and mood disorder was negatively related to the quality of life scale.

법랑아세포종에서의 p53 및 MIB-1 발현 : 임상 및 병리학적 요인들간의 연관성 Correlation Between Clincopathologic Parameters

이수운,한지용,권창석,윤혜경,김우형,이희철 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Thirty five patinets' paraffin embedded biops block , which was previously diagnosed ameloblastoma and obtained from oral and maxillofacial surgery dept. of pusan paik hospital and OMS dept of collage dentistry, pusan national university hospital, from January 1994 to march 1999, were performed immunohistochemistry with p-53 and MIB-1. On investigating the expression, correlation between clinical and histologic factor and correlation between prognosis and moleculopathologic property were examined. And we obtained the following results; 1. Age over forty, recurrence rate was 58.5%. 91.4% of the tumor was developed on mandibule, recurrence rate of maxilla area tumor was 66.7% On radiologic and histologic feature, multilocular and follicular type has high recurrence rate but no significance statistical difference was found 2. Expression of p-53 was 25.7%, When underage of forty. location on mandibule, multilocular and plexiform type, the expression of p-53 was somewhat higher and recurred cases of tumor, expression rate was 12.5%. 3. MIB-1 expression rate was 77.1%. When location on mandibule, unilocular, plexiform type tumor and previously recurred case, MIB-1 expression was 87.5% which was somewhat higher than no recurrence case of 74.1%. 4. 33.3.% of positive on MIB-1 have expression of p-53 and all p-53 positive case express MIB-1 positive. All p-53 positive case have high cell proliferation rate. From this result, age and location have closely related with the prognosis of tumor, histologic feature and MIB-1 expression has related with the prognosis of tumor. But p-53 has highly expressed on cell proliferation area, not closely related with the prognosis of tumor.

PH 4.3에서 재광화 용액의 포화도에 따른 인공 탈회된 법랑질의 동력학적 변화

이지숙,노병덕,신수정,이윤,공형규,이찬영 대한치과보존학회 2009 Restorative Dentistry & Endodontics Vol.34 No.1

법랑질의 재광화에 영향을 주는 요인으로는 PH, 불소 농도, 포화도 등이 있다. 본 연구에서는 유산 완충 탈회용액을 이용하여 법랑질 시편을 인공 탈회시킨 후, pH 4.3에서 포화도를 0.22, 0.30, 0.35로 달리한 세가지 재광화 용액에 10일간 처리하여 나타나는 변화를 편광현미경으로 관찰하여 전체 탈회 깊이와 건전 표층 폭의 변화를 조사하였다. 또한 Image program (Scion Image analyzer)을 이용하여 병소 부위의 평균 mineral density를 측정하여 탈회와 재광화 후 무기질 변화량을 통해 인공 탈회된 법랑질의 동력학적 변화를 관찰하였다. 1 재광화 후 모든 군에서 건선 표층의 폭이 증가하였는데, 포화도가 증가할수록 건전 표층 폭이 증가하였다 (P< .05) 2 재광화 후 mineral density 변화를 관찰한 결과 포화도가 낮은 군에서는 이온의 침착이 병소 전반적으로 일어났으나, 포화도가 높은 군에서는 건전 표층 부위와 표층하 병소의 하층부에서 이온의 침착으로 mineral density 가 증가하였고 표층하 병소의 상층부에서는 탈회가 진행되어 mineral density가 감소하였다. 3 재광화 후 모든 군에서 무기질량이 증가하였고 전체 탈회 깊이도 증가하였으나 각 군간에 통계적 유의차는 없었다. 본 실험에서 인공 탈회된 시편을 pH 4.3인 재광화 용액에 처리시 포화도가 높을수록 건전 표층에서 더 많은 재광화 현상이 일어났고 표층하 병소에서는 재광화 현상이 적게 일어났으며 재광화 후 모든 군에서 전체 탈회 깊이는 증가하였다. The purpose of this study is to observe and compare the dynamic change of artificially demineralized enamel by remineralization solutions of different degrees of saturation at pH 4.3 In this study, 30 enamel specimens were demineralized artificially by lactic acid buffered solution. Each of 10 specimens was immersed in pH 4.3 remineralization solution of three different degrees of saturation (0.22, 0.30, 0.35) for 10 days. After demineralization and remineralization, images were taken by a polarizing microscope (× 100). The density of lesion were determined from images taken after demineralization and remineralization. During remineralization process, mineral deposition and mineral loss occurred at the same time. After remineralization total mineral amount and width of surfacc lesion increased in all groups. The higher degree of saturation was, the more mineral deposition occurred in surface lesion and the amount of mineral deposition was not much in subsurfacc lesion. Total demineralized depth increased in all groups.

불안정 지지면을 이용한 운동 프로그램이 뇌졸중 환자의 균형에 미치는 효과

이지연,김용수,김진섭,박진현,최윤희,전덕훈,김경 대구대학교 특수교육재활과학연구소 2010 再活科學硏究 Vol.28 No.1

본 연구의 목적은 뇌졸중 환자의 재활을 위하여 불안정 지지면을 이용한 운동 프로그램이 균형 능력과 운동 기능 능력에 미치는 효과를 알아보고자 실시하였다. 불안정 지지면의 이용한 치료는 임상적으로 뇌졸중으로 인한 편마비 환자뿐만 아니라 스포츠 손상 및 노인들의 질환 등 다양한 병인을 가진 환자들의 치료에 이용되고 있다. 대부분의 뇌졸중 환자들은 재활 훈련을 할 수 있는 병원이나 기관에서 치료를 받을 때에 지지면의 차이를 제공함으로써 보다 더 안정한 지지면에서 적응할 수 있는 방법이 요구되고 있다. 하지만, 아직도 불안정 지지면을 이용한 구체화된 치료적 운동 방법과 객관적 평가에 대한 연구가 미비한 실정이다. 이러한 문제점을 해결하기 위하여 최근 뇌졸중 환자의 불안정 지지면과 이론적 근거를 바탕으로 한 운동 프로그램에 대한 연구가 더욱 필요하다. 본 연구에서는 실험군과 대조군이 같은 운동 프로그램을 적용하였을 때, 두 그룹 간에서 나타나는 균형 향상과 운동 기능 향상에 어떠한 효과를 주는지 살펴보고자 하였다. The purpose of this article was to study the effect of the exercise programme that uses the unstable supporting surface to rehabilitate cerebrovascular patients on balance ability and exercise function ability. The therapy is being clinically used for the patients with diverse pathological factors such as hemiplegia caused by CVA, sports injury, and diseases in the elderly. when most patients with CVA are treated in the hospital or institute that can provide rehabilitation training, they need to be given the way to adjust more stable surpporting surface by being provide with various stages of surface. However, the actual therapeutic exercises is still needed and the study for objective assessments wants conducting. To solve these problems, the study of unstable surface exercise programmes based on the evidences recently need to be made for the patients with CVA. in this study, we intend to look into what effects on the balance improvement and the exercise function are different from the experimental group to the control group with the same programme applied to the two groups.

내시경적 조임근절개술후에 발생한 췌십이지장 가성동맥류 1예

박수영,김윤정,박진한,전현수,박시형,이영진,이은지,김지현 仁濟大學校 白病院 2010 仁濟醫學 Vol.31 No.-

We reported a case of pancreatic pseudoaneurysm, involving pancreaticoduodenal artery, developed after endoscopic retrograde cholangiopancreatography(ERCP) with endoscopic sphinterotomy. A 54 year old woman was transferred to our hospital due to sustained hematochezia and hypovolemia. A week before admission, she underwent ERCP with endoscopic sphinterotomy for acute cholangitis in local clinics. She developed abdominal pain and hematochezia at 12 hours after the procedures. A post procedure contrast enhanced computed tomography of abdomen demonstrated a pseudoaneurysm in inferior portion of pancreatic head. This was not present on the pre procedure contrast enhanced computed tomography of abdomen and was thought to arise from the pancreaticoduodenal artery as a complication of the pre sphincterotomy. She was brought to our emergency room in a state of hypovolemia. A upper endoscopy showed active bleeding from ampula of vater. An celiac angiography demonstrated pancreatic pseudoaneurysm involving inferior pancreaticoduodenal artery and selective coil embolization of was performed.

슬릿형 댐퍼를 이용한 구조시스템의 구조적 거동

이수진,박지영,오상훈,윤영호 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

This study was to investigate the structural behavior of braced-frame systems with slit damper. For this study, push-over analysis was carried out to investigate the difference of structural behavior between the braced frame system without slit-plate dampers and the braced frame system with slit-plate dampers. The braced-frame systems with slit plate dampers were more effective than the braced-systems without slit-plate dampers: (1) The plastic hinges which were happened at damper were decentralized each story so that the plastic deformation of the main frames were decreased. (2) The ductilities of the former structure were increased.

보행 시 노인의 상체 움직임에 대한 3차원적 분석

김희수,윤희중,류지선,김태삼 한국운동역학회 2004 한국운동역학회지 Vol.14 No.3

H. S. KIM, H. J. YOON, J. S. RYU, T. S. KIM. The Three Dimensional Analysis of the Upper Body's Segments of the Elderly during Walking. Korean Journal of Sport Biomechanics, Vol. 14, No. 3, pp. 1-15, 2004. The purpose of this study was to investigate the kinematic variables of the upper part of the body for 8 elderly men during walking. For this study, kinematic data were collected using a six-camera (240Hz) Qualisys ProReflex system. The room coordinate system was right-handed and fixed in space, with righted orthogonal segment coordinate systems defined for the head, trunk, and pelvis. Based on a rigid body model, reflective marker triads were attached on the 3 segments. Three-dimensional Cartesian coordinates for each marker were determined at the time of recording using a nonlinear transformation(NLT) technique with ProReflex software (Qualisys, Inc.). Coordinate data were low-pass filtered using a fourth-order Butterworth with cutoff frequency of 6Hz. Three-dimensional angles of the head, trunk, and pelvis segment were determined using a Cardan method. On the basis of each segment angle, angle-angle plot used to estimated the movement coordinations between segments. The conclusions were as follows; (1) During the support phase of walking, the elderly people generally kept their head the flexional and abductional posture. Particularly, the elderly displayed little internal/external rotation. (2) The elderly people showed extensional and external rotation postures in the trunk movement. Particularly, It showed the change from adduction into abduction at the heel contact event of the stance phase. (3) The elderly people showed almost same pelvis movement from the flexion into extension, from the abduction into adduction, and from internal rotation into external rotation at the mid stance and toe off of the stance phase.