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Man Il Kim,Jee Yeon Baek,Min Jee Kim,Heon Cheon Jeong,Ki Gyoung Kim,Yeon Soo Han,Iksoo Kim 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05
The 15,389-bp long complete mitogenome of the endangered red-spotted apollo butterfly, Parnassius bremeri (Lepidoptera: Papilionidae) was determined. This genome has a gene arrangement identical to those of all other sequenced lepidopteran insects, which have the gene order of tRNAMet, tRNAIle, and tRNAGln at the beginning. Due to the uncertainty the start codon for COI gene in insect has been discussed extensively. We propose the CGA sequence as the start codon for COI gene in lepidopteran insects, based on complete mitogenome sequences of lepidopteran insects including our P. bremerii and additional sequences of the COI start region from a diverse taxonomic range of lepidopteran species (a total of 51 species belonging to 15 families). As has been suggested in other sequenced lepidopteran insects the 18 bp-long poly-T stretch and the downstream conserved motif ATAGA that were previously suggested to serve as a structural signal for minor-strand mtDNA replication also was found at the 3’-end region of the P. bremerii A+T-rich region. In an extensive search to find out tRNA-like structure in the A+T-rich region, each one tRNATrp-like sequence and tRNALeu (UUR)-like sequence were found in the P. bremeri A+T-rich region, and most of other sequenced lepidopteran insects were shown to have tRNA-like structure within the A+T-rich region, thereby indicating that such feature is frequent in the lepidopteran A+T-rich region. Phylogenetic analysis using the concatenated 13 amino acid sequences and nucleotide sequences of PCGs of the four macrolepidopteran suferfamilies together with Tortricoidea and Pyraloidea well recovered a monophyly of Papilionoidea and a monophyly of Bombycoidea. However, Geometroidea and Noctuoidea were unexpectedly clustered as one group and placed this group to the sister group to Bombycoidea, instead of Papilionoidea in most analyses.
Kim Min Jee,정수연,Kim Sung-Soo,Jeong Jun Seong,Kim Jong Seok,Jeong Heon Cheon,Kim Ki-Gyoung,Kim Iksoo 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.2
The dung beetle Copris tripartitus (Coleoptera: Scarabaeidae) has long been considered an endangered insect in South Korea; the detection of recent population increases leaves its endangered status uncertain. Population genetic analysis subsequent to development of molecular markers is essential for establishing proper conserva tion strategies. In this study, we developed ten microsatellite markers specifically for C. tripartitus. Sixty-eight individuals of C. tripartitus collected from six South Korean localities were genotyped to validate these markers and preliminarily assess population genetic characteristics. Per-locus observed number of alleles, observed heterozygosity (H O ), and expected heterozygosity (H E ) ranged from 5–12, 0.499–0.958, and 0.54–0.743, respectively. All populations showed higher H O than H E , negative values of inbreeding coefficient, and, overall, no sign of recent population bottlenecks (excluding one population, Seosan). This suggests that C. tripartitus did not suffer from genetic drift and inbreeding, which are typically severe in small, isolated pop ulations. Nevertheless, detection of only one of the two gene pools in some populations and resultant genetic subdivision into two population groups may suggest that the population size is not enough to cover both gene pools. Thus, a more extended period of protection may be required to ensure higher genetic diversity of wide spread populations and achieve the long-term conservation goal.
Kim, Man-Il,Wan, Xinlong,Kim, Min-Jee,Jeong, Heon-Cheon,Ahn, Neung-Ho,Kim, Ki-Gyoung,Han, Yeon-Soo,Kim, Ik-Soo Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.30 No.5
The molecular phylogenetic relationships among true butterfly families (superfamily Papilionoidea) have been a matter of substantial controversy; this debate has led to several competing hypotheses. Two of the most compelling of those hypotheses involve the relationships of (Nymphalidae + Lycaenidae) + (Pieridae + Papilionidae) and (((Nymphalidae + Lycaenidae) + Pieridae) + Papilionidae). In this study, approximately 3,500 nucleotide sequences from cytochrome oxidase subunit I (COI), 16S ribosomal RNA (16S rRNA), and elongation factor-1 alpha (EF-1${\alpha}$) were sequenced from 83 species belonging to four true butterfly families, along with those of three outgroup species belonging to three lepidopteran superfamilies. These sequences were subjected to phylogenetic reconstruction via Bayesian Inference (BI), Maximum Likelihood (ML), and Maximum Parsimony (MP) algorithms. The monophyletic Pieridae and monophyletic Papilionidae evidenced good recovery in all analyses, but in some analyses, the monophylies of the Lycaenidae and Nymphalidae were hampered by the inclusion of single species of the lycaenid subfamily Miletinae and the nymphalid subfamily Danainae. Excluding those singletons, all phylogenetic analyses among the four true butterfly families clearly identified the Nymphalidae as the sister to the Lycaenidae and identified this group as a sister to the Pieridae, with the Papilionidae identified as the most basal linage to the true butterfly, thus supporting the hypothesis: (Papilionidae + (Pieridae + (Nymphalidae + Lycaenidae))).
최근 30년간 국내 논문에 보고된 납중독증에 대한 고찰
김지연,김종혁,김현우,노지호,이관행,천병철,남상민 대한내과학회 2004 대한내과학회지 Vol.66 No.6
배경 : 납은 우리 주변 환경에 널리 존재하며 오랜 시간 동안 여러 용도로 사용되면서 많은 납중독 사례가 보고 되었다. 특히 우리나라를 비롯한 주변동남아 국가에서는 한약에 의한 비직업성 납중독이 보고 되고 있다. 방법 : 1973년부터 2002년까지 Medric을 통하여 검색한 납중독 관련 논문들과 이들의 참고문헌을 바탕으로 총 45예에 대한 자료를 수집하였고, 이들에 대하여 다음과 같은 특성으로 분류하였다. 1) 보고연도, 2) 연령, 3) 성별, 4) 노출경로, 5) 노출기간, 6) 발현된 중독 증상, 7) 치료 결과 : 전체 환자들의 평균 연령은 41.4세였고, 성별에 따른 분포는 큰 차이가 없었다. 비직업적 납중독은 대부분 한약에 의한 경우들이었다. 한약에 의한 납중독의 경우 한양의 평균 복용기간은 7.3±3.8개월이었고, 복용량은 5 ㎎∼3 g까지 다양하였다. 납중독 환자들의 임상증상은 주로 복통, 두통, 변비 등이었다. 특징적인 실험실 소견으로 빈혈이 관찰되었다. 주 치료 약제는 경구 penicil lamine이 사용되었다. 결론 : 납중독은 임상에서 비슷한 증상을 보이는 유사한 질환이 많아 진단하기에 어려운 면이 있다. 특히 비직업성 납중독의 경우는 더욱 그러하다. 우리나라에서는 서양과는 달리 한약제에 의한 비직업성 납중독이 다수 발견되어 왔다. 본 저자들은 이러한 납중독의 예방과 관리를 위해 건강보조식품이나 한약(특히, 한약류) 등의 과학적이고 합리적인 제조 및 향후 관리가 필요하다고 생각하게 되었다. Background : Because of widespread and long-term use of lead, many lead poisoning cases were reported. Especially, in Korea including Southeast Asia, we observed non-occupational lead poisoning cases by herbal medication. Methods : We identified total 45 cases of lead poisoning through Medric search, from 1973 to 2002. Bibliographies of relevant articles were reviewed. We arranged cases by attributes following 1) reported year, 2) age, 3) sex, 4) exposure sources, 5) exposure duration, 6) clinical manifestation, 7) treatments. Results : The average age of the patients was 41.4±15.7 year-old. The distribution by sex did not have difference each other. The non-occupational lead poisoning cases were mostly caused by Chinese herbal medication. On the cases of the herbal lead poisoning, average exposure duration was 7.3±3.8 months and the various dosages were taken from 5 ㎎ to 3 g. The major clinical symptoms were abdominal pain, headache, and constipation. The typical laboratory finding was anemia. Major treatment agent was oral penicillamine. conclusion : To differential diagnose of the lead poisoning from many diseases with similar symptoms is very difficult solely on the clinical aspect. Especially, in non-occupational cases it is more difficult. In Korea, non-occupational lead poisoning cases by herbal medication have been discovered more than in western countries. We reviewed about the lead poisoning cases since 1973, so we want to make an aware of scientific and rational making and management of lead in the health supplementary food and herbal medicine.
Kim, Jee Wook,Lee, Boung Chul,Lee, Dong Young,Seo, Cheong Hoon,Kim, Suryong,Kang, Tae-Cheon,Choi, Ihn-Geun Pergamon Press ; Oxford University Press 2013 Alcohol and alcoholism Vol.48 No.1
<P>To identify an optimal brief version of the Alcohol Use Disorders Identification Test (AUDIT) and to evaluate its effectiveness as a screening test for problem drinking (PD), alcohol use disorders (AUD) and alcohol dependence (AD).</P>
Cheon-Woong Choi,Jee-Hong Yoo,Sung-Eun Kim,Yun-Hee Sung,Jin-Hee Seo,Mal-Soon Shin,Dong-Hee Kim,Chang-Ju Kim 대한스트레스학회 2011 스트레스硏究 Vol.19 No.1
폐는 질소종류에 다양하게 반응한다. 폐 상피세포는 nitric oxide synthase (NOS)에 의해 L-아르기닌으로부터 합성된 유리기 무기물 가스인 산화질소에 민감하게 반응한다. 산화질소(nitric oxide)는 많은 생물학적 과정의 매개체이지만, 과도한 출현은 다양한 세포 유형의 세포사멸을 일으키기도 한다. 녹차의 주성분이며, 가장 풍부하게 들어있는 (-)-epigallocatechin-3-gallate (ECGC)는 최근에 세포사멸 경로를 조절하는 것으로 알려졌다. EGCG는 항산화, 항염증, 보호기전, 항세포사멸 효과를 보여주고 있다. 이에 본 연구에서는 인간 폐 상피세포에서 EGCG가 sodium nitroprusside (SNP)로 유도된 세포사멸에 대한 보호효과를 연구하였다. 인간의 폐포 유형 II L-132 세포를 가지고, EGCG의 항세포사멸을 확인하기 위해 MTT assay, DAPL staining, TUNEL assay, DNA fragmentation assay, Western blotting으로 분석하였다. 본 실험의 결과 SNP는 인간의 폐 상피 세포인 L-132에서 세포사멸의 형태학적 변화를 유발하였고, 세포사멸 전단계인 Bax 단백질의 발현을 증가시켰으며, caspase-3의 활성을 증가시켰다. 그러나 EGCE를 처치한 세포에서는 Bax 단백질의 발현을 감소하였고, 반 세포사멸 단백질인 Bcl-2의 발현은 현저하게 증가하여 caspase-3의 활성을 억제하는 결과를 확인하였다. 따라서 본 연구 결과 EGCG는 폐 상피 세포인 L-132에서 SNP로 유도된 세포사멸에 대한 보호 효과를 나타냄을 확인할 수 있었다. The lung is exposed to various stimulants, such as reactive nitrogen species. Alveolar epithelial cells are sensitive to nitric oxide (NO), which is a free radical inorganic gas synthesized from L-arginine by nitric oxide synthase (NOS). NO mediates many biological processes, but excessive NO exposure induces apoptosis in various cell types. (-)-Epigallocatechin-3-gallate (EGCG) has recently been shown to modulate apoptotic pathways. EGCG is the most abundant and most active ingredient of green tea. EGCG has been shown to have anti-carcinogenic, anti-oxidant, anti-inflammatory, neuroprotective, and anti-apoptotic effects. In the present study, we investigated whether EGCG exhibits a protective effect against apoptosis induced by the NO donor sodium nitroprusside (SNP) in human lung epithelial cells. To confirm the anti-apoptotic properties of EGCG, a 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, 4,6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay, DNA fragmentation assay, and Western blotting were performed using human alveolar type II L-132 cells. The present results show that SNP induced apoptotic morphological changes, increased the expression levels of the pro-apoptotic protein Bax and enhanced the enzymatic activity of caspase-3 in L-132 human lung epithelial cells. However, EGCG treatment remarkably increased the expression of the anti-apoptotic protein Bcl-2, decreased the expression of Bax, and suppressed the enzymatic activity of caspase-3. The results of the present study revealed that EGCG exerts a protective effect against SNP-induced apoptosis in L-132 human lung epithelial cells. (Korean J Str Res 2011;19:69∼77)