The Blockade of IL6 Counterparts the Osmolar Stress-Induced Apoptosis in Human Conjunctival Epithelial Cells

Ju, Hee-Jung,Byun, Yong-Soo,Mok, Jee-Won,Joo, Choun-Ki Hindawi Publishing Corporation 2016 Journal of ophthalmology Vol.2016 No.-

<P>To determine the effect of hyperosmolarity on cell survival/apoptosis of conjunctival epithelial cells and evaluate the possible role of IL6, Wong-Kilbourne derivative of Chang conjunctival cell line (WKD) was used in this study. Confluent cells were incubated under different osmolarity (290 mOsm and 500 mOsm) with or without neutralizing IL6 antibody (50 ng/mL). The expression of IL6 level was measured in the supernatant of each conditioned medium. Cell viability/apoptosis assay was performed using Annexin V/Propidium Iodide (PI) and Cell Counting Kit-8 (CCK-8). Western blot was conducted to measure the abundance of apoptotic markers and IL6 related downstream signaling pathway. The concentration of IL6 showed time-dependent increase in cells treated with 500 mOsm. Although apoptosis of WKD cell is increased in treated 500 mOsm for 24 h, apoptosis reduced in WKD cell treated 500 mOsm with anti-IL6 for 24 h. Anti-IL6 inhibited the activation of JAK-STAT signaling pathway, which was induced by hyperosmolarity. Hyperosmolar condition induced apoptosis in conjunctival epithelial cells, along with increase of IL6 production. IL6 neutralizing antibody inhibited apoptosis and JAK-STAT signaling in hyperosmolar condition. These findings suggested that IL6 may be involved in apoptotic change and in hyperosmolarity.</P>

Feasibility of CD147 for an Eruption-Related Molecule during Rat Molar Development

Jee-Hae Kang(강지혜),Joon-Yong Jung(정준용),Hyun-Jin Kim(김현진),Hyun-Mi Ko(고현미),Ji-Yeon Jung(정지연),Min-Seok Kim(김민석),Won-Jae Kim(김원재),Eun-Ju Lee(이은주),Sun-Hun Kim(김선헌) 대한체질인류학회 2009 대한체질인류학회지 Vol.22 No.3

발생기 치아 이돋이는 이돋이 관련 물질의 조절 아래 이루어지며 따라서 이돋이 관련 물질의 구명은 이 분야의 연구에서 주된 과제가 되어 왔다. 발생기 이돋이 관련 물질은 치아주머니세포로부터 유리되며 뼈파괴세포의 형성과 관여하는 것으로 알려져 있다. 그러나 이틀뼈를 포함한 주위 조직의 흡수가 이돋이경로 형성을 위하여 필수적이다. 따라서 치아주머니세포로부터 바탕질분해 관련 단백질이 분비되어 이돋이경로 형성에 관여하리라 가정된다. 또한 법랑질모세포와 상아질모세포가 이돋이 관련 물질을 분비하여 인접 조직에 영향을 미침으로써 이돋이를 도울 수 있다. 본 연구는 현재까지 치아에서 보고된 바 없는 바탕질단백질의 일종인 CD147과 바탕질분해효소의 치아 이돋이 과정에서 역할을 구명하고자 시행되었다. 출생 후 흰쥐 3, 6, 9일에서 발생기 위턱 둘째 및 셋째 어금니를 적출하여 면역조직화학염색과 RT-PCR을 이용하여 발현을 평가하였다. 발생중인 치아 상방에 출현한 뼈파괴세포는 이돋이 상태를 평가하기 위하여 조사되었다. CD147 mRNA와 mmp-2 및 mmp-9 mRNA는 발생 9일에 뿌리형성기에 있는 둘째어금니에서 모자시기에 있는 셋째어금니보다 발현양이 훨씬 많았다. 또한 mmp-2 및 mmp-9 발현은 둘째어금니 발생 3 (후기 종시기), 6 (치아머리시기), 9일(뿌리형성기)에 발생시간 의존적으로 증가되었다. 대조적으로 동일 시기에 mmp-13 mRNA 발현 변화는 관찰되지 않았다. 면역조직학적 관찰에서 CD147은 법랑질모세포와 상아질모세포 외에 치아주머니세포와 뼈파괴세포에서 강하게 발현되었다. 둘째어금니 교합면 위 이틀뼈에 출현한 뼈파괴세포 수는 뿌리형성기에서 유의하게 증가하였다. 이상의 결과로 보아 CD147은 mmp-2 및 mmp-9과 함께 치아 이돋이경로 형성에 관여할 것으로 생각된다. Understanding the genetic control of tooth eruption is one of the major issues in tooth development. Thus far, it is known that eruption-related molecules are secreted from follicular cells surrounding the germs and are related mainly to osteoclast formation. This study examined the involvement of CD147 and its downstream molecules in the eruption of rat developing molars using immunohistochemistry, RT-PCR and histomorphometry. CD147 was expressed differentially in the cap (3rd molar germs) and root formation (2nd molar germs) stages in tooth development. CD147 was localized immunohistochemically in the follicular cells and osteoclasts as well as in the ameloblasts and odontoblasts. The expression pattern of CD147 and mmps was investigated because CD147 is an mmp inducer. The expression of both mmp-2 and -9 increased at the root formation stage compared to that at the cap stage and increased in a stage dependent manner. However, the level of mmp-13 was not changed notably. The histomorphometrical study suggested that the number of osteoclasts that appeared occlusal to the molar germs for the resorption of alveolar bone increased significantly during development. These results suggest that CD147 may play an important role in the formation of the eruption pathway along with the mmps.

Various Types of Stress Deteriorate Spatial Memory through Inhibition of Cell Proliferation in the Hippocampus of Rats

Jee-Sang Yoon,Hyung-Hwan Baik,Su-Shin Lee,Yu-Mi Heo,Bo-Kyun Kim,Mal-Soon Shin,Sang-Won Lee,Chang-Ju Kim 대한스트레스학회 2011 스트레스硏究 Vol.19 No.3

스트레스는 항상성과 체내 균형을 교란시키는 물리적, 생리적, 그리고 정신적 변화를 의미한다. 스트레스는 우울증, 뇌졸중, 그리고 알쯔하이머 병 등 여러 뇌 질환을 유발 시키는 인자로 알려져 있다. 해마는 스트레스에 민감한 뇌의 부위이다. 본 연구에서는 구속 스트레스, 소음 스트레스, 그리고 추위 스트레스 등 여러 종류의 스트레스가 공간기억과 해마에서의 세포 생성에 미치는 영향을 실험하였다. 본 실험에서는 8-arm maze test, 면역조직화학법, 그리고 웨스턴 블롯을 사용하였다. 각각의 스트레스는 하루 1시간씩 2회, 10일간 적용하였다. 본 실험결과 구속 스트레스, 소음 스트레스, 그리고 추위 스트레스는 공간기억을 감소시켰다. 또한 구속 스트레스, 소음 스트레스, 그리고 추위 스트레스는 해마에서의 신경세포 생성을 억제하였다. 이러한 스트레스의 작용은 brain-derived neurotrophic factor (BDNF)와 tyrosine kinase receptor B (TrkB)의 발현을 억제함으로써 일어났다. 본 실험을 통하여 구속 스트레스, 소음 스트레스, 그리고 추위 스트레스 모두 신경세포의 생성을 억제하여 기억력 감퇴를 유발시킴을 알 수 있었다. 이 세가지 스트레스 중 신경세포생성을 억제하고 공간기억력을 감퇴시키는 작용은 구속 스트레스>소음 스트레스>추위 스트레스의 순으로 나타났다. Stress is defined as physical, physiological, and psychological modifications that disrupt the homeostasis and internal balance of the body. Stress is closely associated with neuronal changes such as impaired neurogenesis, neuronal atrophy, and neuronal apoptosis in the hippocampus. In the present study, we investigated the effects of various types of stress (restraint stress, noise stress, cold stress) on spatial memory, cell proliferation, and expression of brain-derived neurotrophic factor (BDNF) and its receptor tyrosine kinase B (TrkB) in the hippocampus of rats. The rats in the restraint stress group were restrained in a transparent cylinder. The rats in the noise stress group were exposed to a 120 dB supersonic machine sound. The rats in the cold stress group were exposed to a cold chamber at 4˚C. The rats in the control group were left undisturbed. Application of each stress was conducted for 60 min 2 times a day, and continued for 10 days. In the present results, all three types of stress induced spatial memory impairment. All three types of stress suppressed cell proliferation and inhibited expression of BDNF and TrkB in the hippocampus. The strength of the deteriorating and suppressing effects of stresses had the following rank order: restraint stress>noise stress> cold stress. Thus, impairment of spatial memory by stresses may be due to the inhibition of cell proliferation through suppressing BDNF expression in the hippocampus. (Korean J Str Res 2011;19:243∼251)

The Changes of Appearance Properties of Bamboo Knitted Fabric After Loess Dyeing

Jee, Ju-Won The Korean Home Economics Association 2007 International Journal of Human Ecology Vol.8 No.2

In this study, the mechanical properties related to the fabric appearance of well being functional bamboo knitted fabric before and after loess dyeing were evaluated. The mechanical properties of fabrics were measured by KES-F system, that is, shape retention, draping, wrinkle recovery, compression property, and surface properties, and total hand value of three types of knitted fabric, 100% bamboo, 100% cotton, and bamboo / cotton blend (60 / 40) were evaluated before and after loess dyeing. As a result, it was found that appearance density, shape retention, and drape coefficient of cotton knitted fabric were greater than those of bamboo knitted fabric. After loess dyeing, shape retention and drape coefficient, wrinkle recovery of bamboo knitted fabric improved and WC/C and MMD/SMD decreased. According to THV, the hand of bamboo/cotton blend knit is the best among three samples by compensation the weak properties of the two fiber. Therefore, loess dyeing seemed to be a good method for improving shape retention and hand value of 100% bamboo and bamboo blended fabric.

Case Report : A case of Terminal Deletion of Chromosome 10P

( Jee Hae Cho ),( Ji Eun Park ),( Min Kyung Ko ),( Eun Ju Kim ),( Ji Kwon Park ),( Soon Ae Lee ),( Jong Hak Lee ),( Won Young Paik ) 대한산부인과학회 2011 Obstetrics & Gynecology Science Vol.54 No.7

Chromosome 10p deletion (partial monosomy 10p) is rare chromosomal disorder. It was first reported in 1970. Since then, as far as we know, about 45 patients have been described. The main feature of this syndrome are craniofacial dysmorphism, congenital heart disease, vesicoureteral abnormalities, and developmental delay. We have experienced a prenatal case of Chromosome 10p terminal deletion by doing cytogenetic study due to high Down syndrome risk on quadruple test and cleft lip on prenatal ultrasonography. Conventional cytogenetic result from cord blood was 46,XY,del(10)(p13), molecular cytogenetic techniques using bacterial artifi cial chromosome array comparative genomic hybridization and fl uorescence in situ hybridization analysis result was 46,XY,del(10)(p14)(NEBL-). To our knowledge, this karyotype may be the first report in Korea. We present this case with brief review of literature.

A novel mechanism for the pyruvate protection against zincinduced cytotoxicity: mediation by the chelating effect of citrate and isocitrate

Jee-Won Sul,Tae-Youn Kim,Hyun Ju Yoo,Jean Kim,Young-Ah Suh,Jung Jin Hwang,Jae-Young Koh 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.8

Intracellular accumulation of free zinc contributesto neuronal death in brain injuries such as ischemiaand epilepsy. Pyruvate, a glucose metabolite, has beenshown to block zinc neurotoxicity. However, it is largelyunknown how pyruvate shows such a selective andremarkable protective effect. In this study, we sought tofind a plausible mechanism of pyruvate protection againstzinc toxicity. Pyruvate almost completely blocked corticalneuronal death induced by zinc, yet showed no protectiveeffects against death induced by calcium (ionomycin,NMDA) or ferrous iron. Of the TCA cycle intermediates,citrate, isocitrate, and to a lesser extent oxaloacetate, protectedagainst zinc toxicity. We then noted with LC–MS/MS assay that exposure to pyruvate, and to a lesser degreeoxaloacetate, increased levels of citrate and isocitrate,which are known zinc chelators. While pyruvate addedonly during zinc exposure did not reduce zinc toxicity,citrate and isocitrate added only during zinc exposure, asdid extracellular zinc chelator CaEDTA, completelyblocked it. Furthermore, addition of pyruvate after zincexposure substantially reduced intracellular zinc levels. Our results suggest that the remarkable protective effect ofpyruvate against zinc cytotoxicity may be mediated indirectlyby the accumulation of intracellular citrate andisocitrate, which act as intracellular zinc chelators.

Establishment and characterization of bortezomib-resistant U266 cell line: Constitutive activation of NF-κB-mediated cell signals and/or alterations of ubiquitylation-related genes reduce bortezomib-induced apoptosis?

( Ju Won Park ),( Eun Kyung Bae ),( Chan Su Lee ),( Jee Hye Choi ),( Woo June Jung ),( Kwang Sung Ahn ),( Sung Soo Yoon ) 생화학분자생물학회 2014 BMB Reports Vol.47 No.5

Bortezomib has been known as the most promising anti-cancerdrug for multiple myeloma (MM). However, recent studiesreported that not all MM patients respond to bortezomib. Toovercome such a stumbling-block, studies are needed to clarifythe mechanisms of bortezomib resistance. In this study, weestablished a bortezomib-resistant cell line (U266/velR), andexplored its biological characteristics. The U266/velR showedreduced sensitivity to bortezomib, and also showed crossresistanceto the chemically unrelated drug thalidomide. U266/velR cells had a higher proportion of CD138 negative subpopulation,known as stem-like feature, compared to parentalU266 cells. U266/velR showed relatively less inhibitory effectof prosurvival NF-κB signaling by bortezomib. Further analysisof RNA microarray identified genes related to ubiquitinationthat were differentially regulated in U266/velR. Moreover, theexpression level of CD52 in U266 cells was associated withbortezomib response. Our findings provide the basis for developingtherapeutic strategies in bortezomib-resistant relapsed andrefractory MM patients.[BMB Reports 2014; 47(5): 274-279]

Changes in Dimensional stability and Total Appearance Value (TAV) of Wool-blended Fused Fabrics after Pressing and or Dry Cleaning

Jee, Ju-Won 한국의류학회 2003 한국의류학회지 Vol.27 No.12

본 연구에서는 실제 의복 관리와 생산 면에서 접할 수 있는 모 직물의 접착심과의 접착후, 프레싱 처리후, 드라이클리닝 및 프레스로 처리 후 직물의 형태안정성과 의복형성능의 변화를 살펴보았다. 겉감으로는 신사복 춘하용 모 100% 또는 모 혼반 직물 15종류를 사용하였으며 심지로는 신사복에 많이 쓰이는 3종류 심지를 사용하였다. FAST시스템과 KES시스템을 이용하여 직물의 접착 후, 프레싱 처리후, 드라이크리닝과 프레싱 처리 후의 형태안정성과 의복형성능의 변화를 시험하였으며 다음과 같은 결과를 얻었다. 1) 모직물의 형태안정성에서 주용인자인 습윤팽창(HE)은 접착으로 크게 감소하였고 프레싱 처리와 드라이크리닝과 프레싱 처리 후는 접착 후보다 큰 변화를 보이지 않는다. 모직물의 습윤팽창(HE)의 조절시 접착심이 습윤팽창(HE)의 안정성에서 중요한 역할을 하는 것으로 나타났다. 2) 완화수축(RS)은 접착 후 조금 감소하였으나 큰 변화를 보이지 않고 프레싱 처리 후 현저한 감소를 보인다. 드라이크리닝과 프레싱 처리 후는 직물이 수축하고 볼륨감이 생김으로 완화수축(RS)은 더욱 감소하였다. 모직물의 완화수축(RS)의 조절시 프레싱이 중요한 역할을 하는 것으로 나타났다. 3) 모직물의 의복형성능(TAV)은 접착으로 크게 향상되었다. 드라이크리닝과 프레싱 처리 후 직물이 좀더 부드러워지고 볼륨감이 생기면서 의복형성능(TAV)은 적게 향상됨을 보인다. 이와 같이 3가지 처리는 서로 보완되어 모직물의 형태안정성과 의복형 성능을 향상시키는 것으로 나타났다.

High School Students` Perspectives on the Textbook Based on Storytelling

( Jee Hyun Park ),( Mi Ju Kim ),( Jae Won Cheong ),( Hang Yun Cho ),( Sung Sik Chu ),( Seung Ju Lee ),( Won June Moon ),( Sun Jae Park ) 한국수학교육학회 2013 수학교육 학술지 Vol.2013 No.1

The results of TIMSS 2011 shows that mathematical achievement levels of Korean students are one of the highest in the international society. On the back side of it, academic interest and confidence in mathematics is in the lowest level. As a measure to alleviate mathematical achievement levels adhering to its true value, a model mathematics textbook based on storytelling has been developed for high school (Kwon et al, 2012). Since the model textbook was developed by educational researchers and teachers, it needs to be reviewed in the perspective of high school students. Therefore, the research has investigated how high school students interpret the model textbook based on storytelling compared with conventional textbooks. Contents about ‘Probability’, ‘Complex numbers and Quadratic Equations’ from these two types of textbooks were reviewed by high school students. And their thoughts on each of the textbooks were analyzed through surveys and interviews. Survey questions were designed to ask students to compare two textbooks in three themes: explanation methods on mathematical concepts, inquiry process of mathematical principles, and retention effect. The results show that students understand mathematical concepts more deeply through the storytelling textbook, because the textbook explains mathematical concepts in the specific context of the story and students could be actively involved in characters of the story. Since various activities and discussions contained in the storytelling textbook enable students to approach mathematical principles in the bottom-up way rather than the top-down way, students could interpret and apply mathematical principles more easily. The storytelling textbook may require a longer time to make students understand certain materials, but it is expected that it aids the retention of what they have learned.

Outcome and Safety of Nivolumab in Real Life in the Treatment of Far-Advanced Hepatocellular Carcinoma: A Single Center Experience

( Jee Hee Park ),( Joong-won Park ),( Bo Hyun Kim ),( Ju Hee Lee ),( Young Hwan Koh ),( Chang-min Kim ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: Sorafenib has been the only approved systemic agent for advanced hepatocellular carcinoma (HCC) for the past decade and multiple new drugs were recently approved. Out of these, nivolumab, a programmed cell death protein-1 inhibitor, is the first immune checkpoint inhibitor approved for the second-line therapy for advanced HCC. The aim of this study is to describe our experience with nivolumab in patients who have progressed on or been intolerant of prior sorafenib. Methods: Thirty consecutive patients with advanced HCC receiving nivolumab were enrolled between October 2017 and March 2018. Enrolled patients were given nivolumab 3mg/ kg intravenously every 2 weeks. Assessment of response was based on the Immune-Modified Response Evaluation Criteria In Solid Tumors (iRECIST) and RECIEST version 1.1 every 8 weeks. Results: The median age of patients was 65.5 years (range, 46-81 years) and the predominant etiology was hepatitis B (64%). Most patients had preserved liver function (Child-Pugh class A, 93%) and good performance status (ECOG score of 0 or 1, 100%). Portal vein invasion and extrahepatic spread was present in 54% and 67%, respectively. Sorafenib was given all patients as a first-line treatment. Before nivolumab treatment, 6 patients received 2 systemic agents and another 6 patients received 3 or more. As of the cutoff date of April 5 2018, the median duration of nivolumab treatment was 61 days. Eleven out of 30 patients (36.7%) are on treatment, while 19 patients stopped treatment because of disease progression (n=9, 30%), serious toxicity (n=7, 23.3%), treatment refusal (n=2) and follow up loss (n=1). Of 22 patients available for evaluating tumor response, 2 showed partial response (PR; 9.2%); 8 stable response (SD; 36.3%); 4 immune unconfirmed progression (iUPD; 18.2%) and 8 immune confirmed progression (iCPD; 36.3%). One of the four iUPDs was identified as iCPD and discontinued treatment, while three patients are still on treatment awaiting the next evaluation. Median overall survival (OS) was not reached because of short follow up duration (83 days). Mean OS was 117.7 days (95% confidence interval [CI]; 105.4-129.9) and median progression free survival (PFS) was 97 days (95% CI; 72.9-121.1). Survival rate and PFS rate at 3 months were 90.2% and 64.9%, respectively. Most common adverse reactions included abdominal discomfort (20%), general weakness (13%), constipation (13%), ascites (13%), skin eruption or pruritus (13%), anorexia (10%), nausea (10%), myalgia (7%), diarrhea (7%), peripheral edema (7%), fatigue (3%), dyspnea (3%). Treatment-emergent grade 3 adverse events (n=7) included hepatotoxicity, azotemia, hematochezia, anorexia, hyperbilirubinemia, and hypercalcemia, which necessitated treatment cessation. Conclusions: In real life practice of a single center, nivolumab as a second- or a third-fourth-line therapy appears to have meaningful efficacy and acceptable tolerability in patients with far-advanced HCC. Further follow-up studies are warranted.