DNA minor groove에 결합하는 tallimustine (FCE 24517)의 DNA 염기서열 특이성 결합

제강훈,이종순 嶺南大學校 基礎科學 硏究所 1998 基礎科學硏究 Vol.18 No.-

Tallimustine (FCE 24517) is an alkylating benzoyl mustard derivative of distamycin A. The antibiotic distamycin A is DNA minor groove binding agent that recognizes a stretch of at least four ATs. Tallimustine, like distamycin A, is expected to bind preferentially to AT-rich sequences in the minor grove of B-DNA. In this study we have determined covalent binding sites of tallimustine using heat-induced strand cleavage assay. The result shows that tallimustine alkylates alkylates the N3 adenine in the sequences ??(underline indicates the alkylation sites). In spite of the presence of a benzoyl nitrogen mustard group this compound fails to alkylate the N7 position of guanine, the major site of alkylation by conventional nitrogen mustards. It is clear that the alkylated adenine N3 is found only in the hexamer ?? sequences, suggesting that tallimustine has high base and sequence specificities in the minor groove of DNA.

인조 합성 테니스 바닥재의 지면 반력 분석

조승제,정미라,서국웅,박승범,윤양진,이훈식,강영택 釜山大學校 附設 體育科學硏究所 1998 體育科學硏究所 論文集 Vol.14 No.-

Some authors suggest that certain types of surfaces are the origin of such injuries. A few years after the first medical concerns about surfaces were voiced, publications of biomechanical measurements apperared, describing accleration, force, and impact measurement on different types of surfaces. In many sport activities, surfaces can be under very high dynamic load. This was the reason for the development of various methods for impact simulation, like the development of various methods for impact simulation, like the artificial athlete. Furthermore, it is important to collect information about the hardness of new and already existing surfaces in sport arenas. The idea on which this measuring system is based Is as follows: The stiffness of the material can be computed from the kinematics measurd at touch down of a rigid body onto a material sample. The results show the following The result for the artifical surfaces(Synpave ace) is surprising. It is known that these surfaces are much harder than synpave rebound classic, synpave spring. This finding suggests that it may be possible that the subjective impression is used as one criterion in the selection of landing(or style) strategies. The number of subjects in this experiment is too small to make statistically significant conclusions. It is shown analytically that when an object a deformable surface, the acceleration it experiences is inversely propotional to its mass. In future, it need to stress that the interaction between shoe and surface is important, and this aspect has now become well accepted. Considering biomechanical aspect in player's injury, it request Korean Standards for synthetic playing surfaces in sport like ASTM(America Society for Testing & Materials) standards of America, DIN 18035 standards of Germany, BSI standards of U.K.

발열을 동반한 호중구감소증에서 Cefepime 단독투여와 Ceftazidime 및 Tobramycin 병합투여의 효능비교

정현욱,채제욱,강미라,양정채,문치숙,기현균,장현하,오원섭,김기현,백경란,이남용,송재훈 대한감염학회 2004 감염과 화학요법 Vol.36 No.6

목적 : 국내에서 발열을 동반한 호중구감소증이 있는 악성 종양환자에게 경험적 항생제로 베타락탐계 항생제와 아미노배당체의 병합요법의 사용이 일반적이다. Cefepime은 광범위 항균 작용을 가지고 있어, 그람 음성균 뿐만 아니라 그람 양성균에 대해서도 우수한 효과를 나타낸다. 재료 및 방법: 발열을 동반한 호중구감소증이 있는 악성 종양환자를 대상으로 무작위, 공개, 비교 연구를 시행하였다. 대상 환자를 무작위로 cefepime 단독요법군과 ceftazidime 및 tobramycin 병합요법군으로 나누어 투여하고 각각의 임상적 효능과 안전성을 비교하였다. 구강및 인후 점막염이 있는 환자에서 분리된 녹색 연쇄알 구균에 대한 항생제 내성 정도를 조사하였다. 결과 : 대상환자 89명 중 CA 투여군이 48예(53.9%), CT 투여군이 41예(46.1%)이었다. 발열의 유형별로 MDI는 18예(20.2%), CDI는 9예(10.1%), UF는 62예(69.7%)로 두 군 간에 차이가 없었다. CA 투여군과 CT 투여군의 임상적 호전률은 시험약 투여 후 2-4일째 각각 91.7%, 85.4% (P=0.31), 치료 종료 시 각각 91.7%, 100% (P=0.15)로 두 군간에 유의한 차이가 없었다. 치료 종료 시 CA 투여군과 CT 투여군의 세균학적 소실률은 모두 100%로 두 군간의 유의한 차이가 없었다(P=0.78). 점막염이 있는 환자로부터 녹색 연쇄알 구균이 분리된 경우는 25예(28.1%)이었으며, 분리된 녹색 연쇄알 구균은 penicillin, ceftriaxone, cefepime, vancomycin에 모두 감수성을 보였다. 약제 관련 이상 반응의 발생 빈도도 두 군간에 유의한 차이가 없었다. 결론 : 발열을 동반한 호중구감소증이 있는 악성 종양환자의 경험적 항생제로서 cefepime 단독요법은 ceftazidime 및 아미노배당체의 병합요법만큼 효과적이고 안전하였다. Background : Broad-spectrum antibiotic therapy has been recommended as an empirical regimen in cancer patients with febrile neutropenia. Cefepime is a fourth generation cephalosporin with good activity against both gram-positive cocci and gram-negative bacilli. Materials and Methods : To compare the efficacy and safety of cefepime alone with ceftazidime plus tobramycin as empirical regimen for adult cancer patients with febrile neutropenia, a randomized, open label, comparative trial was performed. If the patient showed clinical improvent 72 hours, antibiotic could be changed to oral ciprofloxacin. Clinical and microbiological responses were determined at 72 hours and at the end of therapy. To investigate the antimicrobial resistance of viridans streptococci, swab cultures were obtained from throat in all enrolled patients and antimicrobial susceptibility tests were performed by using microdilution method according to the NCCLS. Results : A total of 89 patients were enrolled. Forty-eight patients received cefepime alone (CA), and 41 patients received ceftazidime plus tobramycin (CT). Demographic and baseline clinical characteristics were similar in both groups (P>0.05). The initial clinical success rate at day 2-4 in group CA (91.7%) was similar with that in CT group (85.4%) (P=0.31). At the end of therapy, the final clinical success rate in CA group (91.7%) was similar to that in CT group (100%) (P=0.15). In 18 patients, with microbiologically defined infections, the eradication rate was 100% in both groups. Adverse events including liver dysfunction (21.3%) and renal dysfunction (2.2%), were similar in both groups (P=0.87). Viridans streptococci were isolated from the throat cultures in 25 cases, and all of these strains were susceptible to penicillin (MIC_(90) 0.12 ㎍/mL), cefepime (1 ㎍/mL), and vancomycin (0.12 ㎍/mL). Conclusion : Efficacy and safety of cefepime monotherapy was comparable to the combination of ceftazidime and tobramycin. It could be used as an alternative empirical regimen for treating cancer patients with febrile neutropenia.

9-12%Cr강의 첨가원소 및 열처리에 따른 미세조직거동과 경도변화

주성욱,유정훈,신기삼,허성강,이재현,석진익,김병훈,김정태 국립7개대학공동논문집간행위원회 2004 공업기술연구 Vol.4 No.-

9-12%Cr steel are getting increasing attention for expanded application as high temperature materials applicable for gas turbine compressors, steam turbine rotors, blade and casing. For efficient and extensive application of 9-12%Cr steel, the data in addition to the improvement of mechanical properties on the correlation of microstructural evolution and mechanical properties are inevitable and thus the investigation in this study is performed for the determination for the specimens heat-treated at 600, 650 and 700℃ for 1000, 3000 and 5000hrs. The microstructure of as-received specimen was tempered martensite, with a high dislocation density and secondary phases. Upon high temperature heat-treatment, the martensite lath got larger and dislocation density decreased resulting the reduction of the strength. In addition, sharp growth of secondary phases was observed in the specimen heat-treated at 700℃ for 5000hrs. A specimen aged at 600℃ and 650℃ Laves phase was observed. The hardness showed close relationship with the secondary phase particle and dislocation density.

Surgical Reconstruction Using a Flanged Mesh Cage without Plating for Cervical Spondylotic Myelopathy and a Symptomatic Ossified Posterior Longitudinal Ligament

Kang, Jung Hoon,Im, Soo-Bin,Yang, Sang-Mi,Chung, Moonyoung,Jeong, Je Hoon,Kim, Bum-Tae,Hwang, Sun-Chul,Shin, Dong-Seong,Park, Jong-Hyun The Korean Neurosurgical Society 2019 Journal of Korean neurosurgical society Vol.62 No.6

Objective : We introduce innovative method of cervical column reconstruction and performed the reconstruction with a flanged titanium mesh cage (TMC) instead of a plate after anterior corpectomy for cervical spondylotic myelopathy (CSM) and an ossified posterior longitudinal ligament (OPLL). Methods : Fifty patients with CSM or OPLL who underwent anterior cervical reconstruction with a flanged TMC were investigated retrospectively. Odom's criteria were used to assess the clinical outcomes. The radiographic evaluation included TMC subsidence, fusion status, and interbody height. Thirty-eight patients underwent single-level and 12 patients underwent two-level corpectomy with a mean follow-up period of 16.8 months. Results : In all, 19 patients (38%) had excellent outcomes and 25 patients (50%) had good outcomes. Two patients (4%) in whom C5 palsy occurred were categorized as poor. The fusion rate at the last follow-up was 98%, and the severe subsidence rate was 34%. No differences in subsidence were observed among Odom's criteria or between the single-level and two-level corpectomy groups. Conclusion : The satisfactory outcomes in this study indicate that the flanged TMC is an effective graft for cervical reconstruction.

The Inhibitory Principle of Lipopolysaccharide-Induced Nitric Oxide Production from Inula Britannica var. Chinensis

Je, Kang-Hoon,Han, Ah-Reum,Lee, Hyun-Tai,Mar, Woong-Chon,Seo, Eun-Kyoung The Pharmaceutical Society of Korea 2004 Archives of Pharmacal Research Vol.27 No.1

A sesquiterpene lactone, 1-O-acetyl-4R,6S-britannilactone (1) isolated from the flowers of Inula britannica L. var. chinensis (Rupr.) Reg. (Compositae), was found as an iNOS inhibitory constituent for the first time with an $IC_{50}$ value of 22.1 $\mu$ M which is more potent than the positive control, L-$N^6$-(1-iminoethyl)lysine ($IC_{50} =33.7 \mu$ M). Structure of compound 1 was identified by 1D and 2D NMR experiments and by comparison with the reference standard.

Hepsulfam Induced DNA Adduts and Its Excision Repair by Bacterical and Mammalin 3-Methyladenine DNA Glycosylases

Je, Kang-Hoon,Son, Jong-Keun,O'Connor, Timothy R.,Lee, Chong-Soon Korean Society of Molecular Biology 1998 Molecules and cells Vol.8 No.6

TERT mRNA Expression is Up-Regulated in MCF-7 Cells and a Mouse Mammary Organ Culture (MMOC) System by Endosulfan Treatment

Je Kang Hoon,Kim Ki Nam,Nam Kung Woo,Cho Myung Haing,Mar Woong Chon The Pharmaceutical Society of Korea 2005 Archives of Pharmacal Research Vol.28 No.3

Endosulfan is one of the organochlorine pesticides, which are well-known endocrine disruptors (EDs), and it acts as an estrogen agonist. Estrogen is a group of hormones that play an important role in mammary gland function and are implicated in mammary carcinogenesis. In the present study, we studied the effects of endosulfan on nodule like alveolar lesion (NLAL) formation in mouse mammary gland development using a mouse mammary gland organ culture (MMOC) system. Although endosulfan-treated mammary glands did not form NLALs, more alveolar buds were formed in this group than in the negative control (vehicle-treated) group. In addition, telomerase reverse transcriptase (TERT) mRNA expression levels were increased in endosulfan-treated mammary glands in a dose-dependent manner. Telomerase can be activated by estrogen, therefore, we examined the effects of endosulfan on telomerase activity, and found that the telomerase activity in estrogen receptor-positive MCF-7 cells was up-regulated by endosulfan treatment. Moreover, this activation was accompanied by the up­regulation of the TERT mRNA expression. Also, transient expression assays using CAT reporter plasm ids containing various fragments of the TERT promoter showed that this imperfect palindromic estrogen-responsive element is almost certainly responsible for the transcriptional activation by endosulfan. These results may help elucidate the endocrine disrupting mechanism of endosulfan.

A STUDY ON THE ARCHITECTURE OF FUTURE MOBILE NETWORK TOWARD INTEGRATED BROADBAND SERVICES

Kyeong-Hoon Kang,Je-Hoon Yoo,Yoon-Ju Lee 대한전자공학회 1995 International Conference on Electronics, Informati Vol.1995 No.8

Rotational vertebral artery syndrome treated via an anterior approach and selective decompression only

Jung Hoon Kang,임수빈,Je Hoon Jeong,Dong-Seong Shin 대한뇌혈관외과학회 2019 Journal of Cerebrovascular and Endovascular Neuros Vol.21 No.3

We present the case of a 38-year-old male who complained of repeated dizziness and syncope. Rotational vertebral artery syndrome (RVAS) was diagnosed via videonystagmoraphy (VNG), computed tomography angiography (CTA) and three-position digital subtraction angiography (DSA). In the neutral position, CTA and DSA revealed left vertebral artery (VA) stenosis at the C2 transverse foramen and right VA hypoplasia. When the head was turned to the right, the blood flow stopped at the C2 level. The bony structure around the VA at the C2 transverse foramen was decompressed via an anterior surgical approach, and the symptoms resolved. This case present the precise stenotic point evaluation by three-position DSA is crucial for the planning of surgical treatment.